{"title":"[Tyrosine-phosphorylated proteins in human breast carcinoma].","authors":"S Bretti, A P Cappa, P M Comoglio, M F Di Renzo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Proteins phosphorylated on tyrosine are detectable by antibodies against phosphotyrosine (P-Tyr antibodies) in cells transformed by oncogene-encoded tyrosine kinases. We used P-Tyr antibodies to investigate the existence of abnormal levels of phosphoproteins in human breast cancer. Three human breast cancer cell lines (SK-BR-3, MCF-7 and CG-5) and 37 human breast cancer specimens were examined by Western blot analysis and \"in vitro\" kinase assay. In the SK-BR-3 cell line three major phosphoproteins of the approximate Mr of 185,000 (p185), 135,000 (p135) and 110,000 (p110) were detected. The former was identified as the HER-2 gene product by specific antibodies against HER-2 encoded protein. In the other cell lines, a product of the approximate Mr of 170,000 (p170), together with a p135 and a p110, were phosphorylated on tyrosine. P185 and p170 were shown to have an associated tyrosine kinase activity. Two proteins, comigrating with p135 and p110, were found to be highly phosphorylated on tyrosine in 50% of the breast cancer samples, but not in samples harvested from 12 human tumors of the gastro-intestinal tract. These data show that 50% of human breast cancer samples display an abnormal level of tyrosine phosphorylated proteins.</p>","PeriodicalId":76654,"journal":{"name":"The Journal of nuclear medicine and allied sciences","volume":"34 4 Suppl","pages":"205-10"},"PeriodicalIF":0.0,"publicationDate":"1990-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12873319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Diagnostic value of prostatic acid phosphatase and prostate-specific antigen in patients with prostatic cancer.","authors":"F Pagani, T Zambolin, R Bonora, M Panteghini","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>To compare the clinical usefulness of the measurement of prostatic acid phosphatase (PAP) and prostate-specific antigen (PSA) in serum of patients with prostatic carcinoma, we studied 128 patients with prostatic pathology, sixty (46.9%) of whom had prostatic cancer. Receiver-operating characteristics (ROC) curves were constructed and the underlying areas were calculated and compared to study clinical efficiency of the two markers regardless of the cutoff level selected. The area for PSA (0.90 +/- 0.30) was significantly higher (p less than 0.001) than that of PAP (0.71 +/- 0.05) showing that PSA was a better discriminator of the patients with or without prostatic cancer. The maximal clinical efficiency of the two tests at selected cutoff levels (0.8 U/L for PAP and 10 micrograms/L for PSA) was 0.787 and 0.883, respectively, confirming the superiority of PSA. However, the associated determination of the two markers improved the clinical specificity with no false-positive cases.</p>","PeriodicalId":76654,"journal":{"name":"The Journal of nuclear medicine and allied sciences","volume":"34 4 Suppl","pages":"85-7"},"PeriodicalIF":0.0,"publicationDate":"1990-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12873324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Tumor markers and cervical carcinoma.","authors":"W Taccone, M Belli, M Riccio","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76654,"journal":{"name":"The Journal of nuclear medicine and allied sciences","volume":"34 4 Suppl","pages":"63-6"},"PeriodicalIF":0.0,"publicationDate":"1990-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13124461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M Gion, G Fila, R Biasioli, G Vignati, R Mione, S Saracchini, E Rinaldi, E Testa, G Bruscagnin
{"title":"Kinetic use of tumor markers in the follow-up of patients operated for primary breast cancer.","authors":"M Gion, G Fila, R Biasioli, G Vignati, R Mione, S Saracchini, E Rinaldi, E Testa, G Bruscagnin","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76654,"journal":{"name":"The Journal of nuclear medicine and allied sciences","volume":"34 4 Suppl","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"1990-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13246889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M Quaranta, G Micelli, M Coviello, A Donadeo, A Lozupone, F Schittulli
{"title":"[Clinical usefulness of CA M26 and CA M29 in breast carcinoma].","authors":"M Quaranta, G Micelli, M Coviello, A Donadeo, A Lozupone, F Schittulli","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76654,"journal":{"name":"The Journal of nuclear medicine and allied sciences","volume":"34 4 Suppl","pages":"35-8"},"PeriodicalIF":0.0,"publicationDate":"1990-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13247432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M Di Seri, A Manna, M Sciò, G G Marchei, A De Benedetto, M G Reale
{"title":"[Evaluation of the new marker CA 549 versus CA 15-3 in neoplastic and non-neoplastic disease of the breast].","authors":"M Di Seri, A Manna, M Sciò, G G Marchei, A De Benedetto, M G Reale","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76654,"journal":{"name":"The Journal of nuclear medicine and allied sciences","volume":"34 4 Suppl","pages":"49"},"PeriodicalIF":0.0,"publicationDate":"1990-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13247437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M Foglia, P G Verri, G M Micca, M Toppino, A Tarani, D Tagliati, A De Pascale, F Corongiu
{"title":"CA 125 serum levels and postpartum.","authors":"M Foglia, P G Verri, G M Micca, M Toppino, A Tarani, D Tagliati, A De Pascale, F Corongiu","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76654,"journal":{"name":"The Journal of nuclear medicine and allied sciences","volume":"34 4 Suppl","pages":"71-4"},"PeriodicalIF":0.0,"publicationDate":"1990-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13247439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Application of lectin histochemistry for the study of uterine and ovarian neoplasms.","authors":"V Bychkov","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76654,"journal":{"name":"The Journal of nuclear medicine and allied sciences","volume":"34 4 Suppl","pages":"191-4"},"PeriodicalIF":0.0,"publicationDate":"1990-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13247557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A Spinazzi, E Soresi, U Borghini, R Boffi, R Vigorelli, S Scoccia
{"title":"Clinical value of neuron specific enolase and tissue polipeptidic antigen for the management of patients with lung cancer.","authors":"A Spinazzi, E Soresi, U Borghini, R Boffi, R Vigorelli, S Scoccia","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76654,"journal":{"name":"The Journal of nuclear medicine and allied sciences","volume":"34 4 Suppl","pages":"141-5"},"PeriodicalIF":0.0,"publicationDate":"1990-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13123825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M A Bassetto, T Franceschi, M Lenotti, F Pancheri, M Zaninelli, A Corgnati, G L Cetto
{"title":"[Value of tumor marker positivity in germinal non-seminomatous tumors of the testis].","authors":"M A Bassetto, T Franceschi, M Lenotti, F Pancheri, M Zaninelli, A Corgnati, G L Cetto","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76654,"journal":{"name":"The Journal of nuclear medicine and allied sciences","volume":"34 4 Suppl","pages":"89-90"},"PeriodicalIF":0.0,"publicationDate":"1990-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12873272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}