{"title":"Decreased activity of commonly measured serum enzymes: causes and clinical significance.","authors":"A A Nanji","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The clinical significance of decreased enzyme activity in serum is less well understood than is the significance of increased activity. This review updates the causes and possible clinical significance of decreased activity in serum of the commonly measured enzymes: alkaline phosphatase, creatine kinase, aspartate and alanine aminotransferases, amylase, lactic dehydrogenase, and gamma-glutamyltranspeptidase.</p>","PeriodicalId":76595,"journal":{"name":"The American journal of medical technology","volume":"49 4","pages":"241-5"},"PeriodicalIF":0.0,"publicationDate":"1983-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17254329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Hematology problem: plasma cell leukemia in multiple myeloma.","authors":"J Parrish","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76595,"journal":{"name":"The American journal of medical technology","volume":"49 4","pages":"263-4"},"PeriodicalIF":0.0,"publicationDate":"1983-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17898261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Case report: potential error in the estimation of antimicrobic concentrations by the microbiological assay method.","authors":"D L Sewell, A C Boudousquie","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An 83-year-old man was admitted for gastrointestinal bleeding that required a segmental colectomy for correction. The patient was initially treated with gentamicin, clindamycin, and cephapirin for sepsis. Antibiotic coverage was changed to amikacin, clindamycin, and penicillin G for sepsis and anaerobic cellulitis. When renal function began to deteriorate, serum amikacin levels were determined by a radioenzymatic and bioassay method. The serum concentration of amikacin in the presence of penicillin G and clindamycin was overestimated when a multiantimicrobic-resistant Escherichia coli was used as the indicator organism in the microbiological assay.</p>","PeriodicalId":76595,"journal":{"name":"The American journal of medical technology","volume":"49 3","pages":"179-81"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17889182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Uses of immunofluorescence in diagnostic virology.","authors":"D Gallo","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76595,"journal":{"name":"The American journal of medical technology","volume":"49 3","pages":"157-62"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17362755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Serological diagnosis of perinatal infections.","authors":"K L Herrmann, J A Stewart","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76595,"journal":{"name":"The American journal of medical technology","volume":"49 3","pages":"149-54"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17362754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Hematology problem: pertussis.","authors":"J H Saumur","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76595,"journal":{"name":"The American journal of medical technology","volume":"49 3","pages":"169-70"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17400886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Isolation and identification of viruses from clinical specimens.","authors":"S Aarnaes, L M de la Maza","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76595,"journal":{"name":"The American journal of medical technology","volume":"49 3","pages":"141-7"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17400885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Differentiation of gram-positive and gram-negative bacteria and yeasts using a modification of the \"string\" test.","authors":"D E Agbonlahor, T O Odugbemi, P O Udofia","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Solutions of potassium hydroxide and sodium hydroxide with concentrations between 0.5% and 4% were found to produce mucoid and sticky suspensions when added in drops, using a bacteriological loop, to smears of 609 strains of gram-negative bacteria on glass slides. A total of 461 strains of gram-positive bacteria and yeasts did not produce any mucoid or \"string\" formation when treated with the same solutions. It was concluded that this modified test could aid in differentiating gram-positive from gram-negative organisms.</p>","PeriodicalId":76595,"journal":{"name":"The American journal of medical technology","volume":"49 3","pages":"177-8"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17400887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Hemoglobin G trait and S trait in the same patient.","authors":"C N LeCrone, J A Jones, J C Detter","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We describe a subject who had a positive sickle solubility test and a routine alkaline electrophoretic pattern showing three major hemoglobulin bands migrating to the A, S, and A2 positions. In addition, the non-heme protein carbonic anhydrase appeared to be increased, suggesting the presence of a split A2. Two major hemoglobin bands separated on agar gel electrophoresis using a citrate buffer, pH 6.2. A non-S hemoglobin migrating to the position of Hb S was identified as GPhiladelphia. The electrophoretic pattern of GPhil/S trait most closely resembles S/beta+ thalassemia or sickle cell trait. Careful study is essential to avoid misdiagnosis and unfairly stigmatizing patients whose benign abnormality may be confused with more severe disease entities.</p>","PeriodicalId":76595,"journal":{"name":"The American journal of medical technology","volume":"49 3","pages":"165-7"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17889180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of urinary proteins on in vitro immunological tests for pregnancy.","authors":"E Cadoff, A Daniel","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A study of the possible interference of urinary proteins with all the commercial in vitro pregnancy products on the market in the United States today was carried out by our group. Six of these tests were of the tube type and seven were slide tests. Radioimmunoassays and receptor assays for beta human chorionic gonadotropin (beta-HCG) were not tested. The products examined showed interferences by urinary proteins presented at various levels. Interference by human serum albumin (HSA) (in five tube tests) occurred only at a physiologically improbable, high concentration of HSA (5 g/dL). However, in certain cases interferences by serum proteins (false positive type) occurred at concentrations lower than 0.06 g/dL which are physiologically probable. The four tests formulated to work with both serum and urine did not show any urinary protein interference with HCG measurements. There was no clear difference in urinary protein interferences between the tube and the slide tests; the tube tests were slightly more sensitive to the presence of HSA. Those tests employing latex beads, as opposed to erythrocytes, showed greater sensitivity to protein interferences.</p>","PeriodicalId":76595,"journal":{"name":"The American journal of medical technology","volume":"49 3","pages":"173-6"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17889181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}