{"title":"Contents of free histidine and histamine in fish meals and in the model compounds and their toxicities to induce gizzard erosion.","authors":"K Kuba, S Miyazaki, Y Umemura","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Whole meal produced from fresh sardine had a high content of histidine, while that from rancid fish had a high content of histamine. The toxicity to induce gizzard erosion, which appeared after heating, was stronger in the former whole meal than in the latter. Thus, the content of histidine, not that of histamine, was well correlate to the degree of toxicity of the heated fish meal. Changes in the contents of histidine and histamine were also studied in model compounds during heating.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"23 2","pages":"69-70"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17735574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Chronological observations on hemato-pathological changes in chicks inoculated with chicken anemia agent.","authors":"T Taniguchi, N Yuasa, M Maeda, T Horiuchi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Chicks were inoculated with the Gifu-1 strain of the chicken anemia agent (CAA) on the day of hatching. They manifested distinct anemia accompanied with pancytopenia 8--20 days after inoculation. Discoloration of the bone marrow and atrophy of the thymus began to be seen 6 days after inoculation. Histologically, hematopoietic cells began to decrease and large blastic cells to appear in the bone marrow 4--6 days after inoculation. Hypoplasia and subsequently aplasia occurred to all over the bone marrow 8 days after inoculation. In the bone marrow erythrocytopoiesis was noticed first 16--18 days after inoculation, granulocytopoiesis later, and transient hyperplasia finally. At last, the bone marrow returned to a normal condition 32 days after inoculation or later. In the thymus the depletion of cortical lymphocytes became distinct 4--6 days after inoculation, and lobular atrophy 8 days after inoculation or later. The depletion of lymphocytes in the other lymphatic tissues and hemorrhage in the lamina propria of the proventriculus were observed only in the anemic phase. The results mentioned above indicated that the anemia induced by CAA was caused by the disorder of hematopoietic cell formation in the bone marrow. It was also noteworthy that cortical lymphocytes in the thymus began to decrease remarkably soon after inoculation.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"23 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17735652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of dietary antibiotics on intestinal microflora in broiler chickens.","authors":"T Ohya, S Sato","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Changes were examined in the intestinal microflora in broiler chickens fed a diet containing antibiotics to obtain fundamental information on the mechanisms of beneficial effect of the antibiotics upon livestock production. Three antibiotics (colistin, bacitracin, and enramycin) were employed as feed additives. Experiments were conducted with broiler chickens in two ways. In one way dietary antibiotics were fed continually at levels approved for use as feed additives for a long term. In the other they were fed the same antibiotics for a short term. Significant changes in microflora were observed mainly in such bacterial groups as aerobic bacteria and Lactobacillus. In the long term administration, three possible modes of variance in the bacterial flora were postulated: Changes directly related to the antibacterial spectrum of antibiotics. Antagonistic changes related to an ecological balance in the bacterial flora. Changes in quantitative balance of bacteria constituting each bacterial group. The change in the intestinal microflora during administration of the antibiotic diet was expressed as a complex form of these transition modes. In the short term administration, it was demonstrated that the effect of the antibiotic diet lingered even 7 days after administration. This suggests that antibiotics used as feed additives may possibly affect the stability of the intestinal microflora.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"23 2","pages":"49-60"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17735657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Reverse plaque formation method for titration of non-cytopathogenic bovine viral diarrhea-mucosal disease virus.","authors":"O Itoh, H Sasaki, T Hanaki","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The reverse plaque formation (RPF) method with a semi-micro plate was applied to the titration of a non-cytopathogenic (non-CP) strain of bovine viral diarrhea-mucosal disease (BVD-MD) virus. All the five non-CP strains used in this experiment formed reverse plaques (RP) on bovine testicle cell culture under methyl cellulose overlay. The RPF was inhibited by the pretreatment of a non-CP virus strain with immune rabbit serum to a reference strain. The specificity of the RPF method was demonstrated by the linear test and Poisson distribution test. Comparative titration of commercial BVD-MD vaccines was carried out by the semi-micro RPF method and the tube method based on the exaltation on Newcastle disease virus. The virus titer obtained by the former was slightly higher than that obtained by the latter. The former was proved to be a method of high sensitivity for determining non-CP virus.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"23 1","pages":"27-31"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17164276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Changes in leukocyte count, blood glucose and coagulation system in calves injected with Escherichia coli endotoxin.","authors":"Y Yagi, Y Nakajima","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Responses of leukocyte, blood glucose and coagulation system in calves were investigated to injection with Escherichia coli endotoxin. Severe leukopenia and hyperglycemia following transient hypoglycemia were noted within 24 hours after injection. In the coagulation system, a definite decrease in platelet count, prolongation of prothrombin time and activated partial thromboplastin time were observed. Fibrinogen, soluble fibrin monomer complex and clotting time, however, varied.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"23 4","pages":"158-60"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17455417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Replication of hog cholera virus in porcine alveolar macrophage cultures.","authors":"S Nakamura, J Sasahara, M Shimizu, Y Shimizu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Eight hog cholera viral strains were tested for virulence for pigs and ability to replicate in porcine alveolar macrophage cultures. Of them, five were virulent and replicated well in the macrophage culture. The other three were avirulent and grew less remarkably in this culture than them. It was suggested that the ability of the hog cholera viral strains to replicate in porcine macrophages might be correlated with their virulence.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"23 3","pages":"101-2"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17731657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Treatment of ovarian cyst in swine by intramuscular injection with luteinizing hormone-releasing hormone analogue.","authors":"A Ogasa, I Domeki, Y Yokoki, S Ito","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The diagnosis of ovarian cyst was made in 53 anestrous sows, which were treated experimentally with luteinizing hormone-releasing hormone analogue (LH-RH-A). Each sow was injected intramuscularly with 200, 400, or 500 micrograms one to five times. She was examined by rectal palpation in the course of treatment. The blood levels of estrogen and progesterone were estimated in three of the treated sows. From changes in them was followed the process of healing of ovarian cyst. As a result, (1) the interval between the beginning of treatment and the appearance of estrus was 26.9 days on the average, the rate of appearance of estrus 60.4%, that of healing of ovarian cyst 71.6%, that of conception 70.2%, and the number of young of a littler 9.0 on the average. (2) In the three sows subjected to estimation, the blood progesterone concentration increased after injection with LH-RH-A to reach almost the same level as that estimated in the corpus-luteum stage. The time of increase after injection was almost identical with the time when the luteinization of ovarian cyst was found by palpation. In a sow which manifested normal estrus, no ovulation took place, but a cyst was formed in the ovary and the blood estrogen concentration maintained at a high level for 10 days after the beginning of estrus.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"23 4","pages":"150-7"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17742124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An electron microscopic study of solar ganglia in piglets infected with Aujeszky's disease virus.","authors":"M Narita, M Osada, A Fukusho","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Ten 2-day-old piglets were inoculated intranasally with Aujeszky's disease virus and their solar ganglia were examined by light and electron microscopy. The cytopathic change noticed was neuronal and glial cell degeneration. It was invariably associated with the virus replicated within the cells. All the main stages of virus development were observed in the degenerated ganglionic cells. Characteristically, plexiform vermicellar array and electron-dense tubular filaments were detected in a part of the nuclei of the infected neuron.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"23 2","pages":"61-6"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17164279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Demonstration of Aujeszky's disease virus antigen in trypsin-digested paraffin-embedded tissue sections by immunofluorescence.","authors":"M Narita, S Inui, M Kobayashi, A Fukusho","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>After trypsin digestion of buffered formalin-fixed, paraffin-embedded brain section, immunofluorescent indication of Aujeszky's disease virus antigen was successful in experimentally infected cow and goat. This result was comparable to those obtained with light and electron microscopic examinations. This method will be diagnostically useful where fresh-frozen tissue is not available.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"23 3","pages":"105-7"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17391044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A study on heifer mastitis in Japan and its causative microorganisms.","authors":"N Seno, R Azuma","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Survey and bacteriological examination were conducted on heifer mastitis in Hokkaido, the northern island of Japan over a period from 1976 to 1981. The disease occurred in heifers, mostly 13 to 18 months old, less than a half of which was pregnant in late summer or in August and September. Infection occurred predominantly in a front quarter than a rear one. Some microorganisms were isolated by the CO2 gas jet method from all the 74 secretions from the affected quarters of 67 heifers. Actinomyces pyogenes ( Corynebacterium pyogenes) was isolated from 49 secretions (66.2%), forty-six of which were involved in mixed infection with other microorganisms, including Peptococcus indolicus that was found in 41 secretions. Streptococcus dysgalactiae was isolated from 13 secretions, from nine of which were obtained pure cultures. The microorganisms harbored in secretion were much more closely associated simultaneously with A. pyogenes and P. indolicus than with A. pyogenes alone or a combination of A. pyogenes and any other microorganism than P. indolicus . This is the first report that P. indolicus was isolated from heifer mastitis in Japan. The \"score\" used for the expression of quality of milk secretion seemed to be useful for the clinical diagnosis of heifer mastitis. The disease in Hokkaido may be etiologically identical with summer mastitis found in the northern European countries.</p>","PeriodicalId":76197,"journal":{"name":"National Institute of Animal Health quarterly","volume":"23 3","pages":"82-91"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17731662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}