African Journal of Microbiology Research最新文献_第10页

Virulence and multi-resistance of gram-negative bacilli strains isolated from some artisanal fermented dairy products sold in secondary schools in Benin 从贝宁中学出售的一些手工发酵乳制品中分离的革兰氏阴性杆菌菌株的毒力和多重耐药性

African Journal of Microbiology Research Pub Date : 2021-04-30 DOI: 10.5897/AJMR2021.9484

Majoie G eroxie Tohoyessou, W. Moussé, Chimène Nanoukon, H. Sina, Akim Socohou, E. Aka, Rodrigue Tollo, F. Baba-Moussa, A. Adjanohoun, L. Baba-Moussa

{"title":"Virulence and multi-resistance of gram-negative bacilli strains isolated from some artisanal fermented dairy products sold in secondary schools in Benin","authors":"Majoie G eroxie Tohoyessou, W. Moussé, Chimène Nanoukon, H. Sina, Akim Socohou, E. Aka, Rodrigue Tollo, F. Baba-Moussa, A. Adjanohoun, L. Baba-Moussa","doi":"10.5897/AJMR2021.9484","DOIUrl":"https://doi.org/10.5897/AJMR2021.9484","url":null,"abstract":"This work aims at assessing toxin production capability and antibiotic resistance profiles of thermo-tolerant Gram-negative bacilli strains isolated from three types of fermented dairy products (yoghurt, degue millet and degue couscous). Samples collected in Abomey-Calavi and Cotonou were analyzed for microbial, biochemical and molecular parameters. Samples were contaminated with thermo-tolerant Gram-negative bacilli strains at 13.88%. The high contamination rate was recorded with the samples of degue couscous and the lowest contaminated samples were degue millet. Morning samples were more contaminated. Klebsiella pneumoniae, Enterobacter aerogenes, Escherichia coli and Enterobacter cloacae were the most identified bacteria. The most isolated species in the dry season was E. coli. In the rainy season, K. pneumoniae and E. cloacae were the most isolated species. K. pneumoniae was the most formative of biofilm (38.9%). About 12% of the isolated strains were extended-spectrum beta-lactamases (ESBL) producers. The higher resistance rate was observed with amoxicillin and doxycylin (92.3%). Imipenem was the most efficient molecule on the isolated strains with 00% of resistance. The blaTEM gene was detected in 51.43% of the isolated strains followed by blaSHV (37.14%), blaCTX-M (8.57%) and blaOXA-1 (2.86%). It is necessary to train sales people on good hygiene practices for food during their production and their distribution. \u0000 \u0000 \u0000 \u0000 Key words: Fermented milk products, thermo-tolerant gram-negative bacilli, toxins, antibiotics resistance, Benin.","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":"15 1","pages":"191-202"},"PeriodicalIF":0.0,"publicationDate":"2021-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46036845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacteriological characteristics and resistance profiles of Vibrio cholerae O1 strains isolated in the Republic of Benin in 2020 2020年在贝宁共和国分离的0型霍乱弧菌菌株的细菌学特征和耐药性概况

African Journal of Microbiology Research Pub Date : 2021-04-30 DOI: 10.5897/AJMR2020.9474

Yves Eric Denon, D. Metodakou, M. Odoun, Alidéhou Jerrold Agbankpé, Victorien Dougnon, Rolande Assogba, Pascaline Mevodjo, Germaine Whannou, Honor e Bankol e

{"title":"Bacteriological characteristics and resistance profiles of Vibrio cholerae O1 strains isolated in the Republic of Benin in 2020","authors":"Yves Eric Denon, D. Metodakou, M. Odoun, Alidéhou Jerrold Agbankpé, Victorien Dougnon, Rolande Assogba, Pascaline Mevodjo, Germaine Whannou, Honor e Bankol e","doi":"10.5897/AJMR2020.9474","DOIUrl":"https://doi.org/10.5897/AJMR2020.9474","url":null,"abstract":"This study aims to carry out a bacteriological characterization and determine the resistance profile of Vibrio cholerae O1 strains isolated during the epidemiological season of 2020 in Benin. To achieve this goal, 43 diarrheal stool samples were analyzed. The samples were taken during the epidemic period of 2020. Bacteriological analyses consisted of enrichment of the samples in buffered peptone water followed by culture on SBCT agar. Then the characteristic colonies were subjected to microscopy, biochemical identification (oxidase, seeding and reading of TSI agar and API 20 E gallery), serotyping, and antibiotic sensitivity tests using the diffusion technique in agar medium according Kirby-Bauer method. The median age of the patients included in this study was 25 years (IQR: 15-40) with predominantly female patients. Individuals aged 11 to 25 were the most represented. Of the 43 stool samples analyzed, 22 were culture positive for V. cholerae and belonged to serogroup O1. The clinical manifestations observed in patients with cholera were watery diarrhea, vomiting and severe dehydration before admission to hospital. It should be noted that all of V. cholerae O1 strains isolated were multidrug resistant with a strong resistance to erythromycin (81.13%), ampicillin (79.96%), chloramphenicol (79.06%), and cotrimoxazole (78.12%). \u0000 \u0000 \u0000 \u0000 Key words: Bacteriological analyses, Vibrio cholerae O1, antimicrobial resistance, Benin.","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":"15 1","pages":"171-177"},"PeriodicalIF":0.0,"publicationDate":"2021-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46080450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of factors fuelling global antimicrobial resistance and its economic and clinical burden 评估助长全球抗菌素耐药性的因素及其经济和临床负担

African Journal of Microbiology Research Pub Date : 2021-03-31 DOI: 10.5897/AJMR2020.9319

Larry L Mweetwa, P. C. Adiukwu, K. Tshiamo, Thatoyaone Kenaope, Tiroyaone Nthusang, E. Oluwabusola

{"title":"Evaluation of factors fuelling global antimicrobial resistance and its economic and clinical burden","authors":"Larry L Mweetwa, P. C. Adiukwu, K. Tshiamo, Thatoyaone Kenaope, Tiroyaone Nthusang, E. Oluwabusola","doi":"10.5897/AJMR2020.9319","DOIUrl":"https://doi.org/10.5897/AJMR2020.9319","url":null,"abstract":"The aim of the research is to assess the factors fuelling Antimicrobial Resistance (AMR) and its economic and clinical burden. For this purpose, a systematic review was conducted, which included an analysis of factors and economic and clinical burden of AMR. It was found that poorly treated sewage; discharges from antibiotic manufacturing plants, empirical prescribing, lack of antimicrobial stewardship, poor AMR monitoring; hospital-acquired infections and human-to-human contacts with environmental pathogens through food; and increase in global trade and travel are the factors behind the spread of AMR. Further, based on previous research, the study found a significant economic and clinical burden caused by AMR infections. It is recommended that well-organised antimicrobial stewardship be in place, AMR monitoring, limit access to antibiotics via over the counter dispensing without prescriptions, mandatory sensitivity tests for antimicrobial prescription be considered, proper hygiene in hospitals and medicine manufacturing sites must be ensured to reduce global AMR. \u0000 \u0000 \u0000 \u0000 Key words: Antimicrobial Resistance, antimicrobial resistance (AMR), antibiotic resistance, economic burden, clinical burden.","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":"15 1","pages":"125-131"},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71092504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-quorum sensing and anti-biofilm activities of Securidaca longepedunculata Fresen, an endangered species from Burkina Faso 布吉纳法索濒危物种长足棘的反群体感应及抗生物膜活性研究

African Journal of Microbiology Research Pub Date : 2021-03-31 DOI: 10.5897/AJMR2020.9440

Issa Karama, Vincent Ouédraogo, AblassÃ© Rouamba, Moussa CompaorÃ, M. Kiendrebeogo

引用次数: 1

Evaluation of yellow fever surveillance in Chad, 2015-2020 2015-2020年乍得黄热病监测评估

African Journal of Microbiology Research Pub Date : 2021-03-31 DOI: 10.5897/AJMR2021.9488

N. Bessimbaye, D. Mbanga, A. Moussa, Djerandouba Yotobumbeti Ferdinand, N. Maxime, N. Barro, A. Tidjani, Choua Ouchemi

{"title":"Evaluation of yellow fever surveillance in Chad, 2015-2020","authors":"N. Bessimbaye, D. Mbanga, A. Moussa, Djerandouba Yotobumbeti Ferdinand, N. Maxime, N. Barro, A. Tidjani, Choua Ouchemi","doi":"10.5897/AJMR2021.9488","DOIUrl":"https://doi.org/10.5897/AJMR2021.9488","url":null,"abstract":"The present study was conducted to determine the seroprevalence of yellow fever virus in patients with clinical signs of febrile jaundice giving suspicion of yellow fever and to evaluate diagnostic techniques for surveillance of yellow fever in the Republic from Chad. From January 2015 to July 2020, an observational study of virological markers was conducted in jaundice patients in Chad. Of the 1730 patients included in this study, a seroprevalence of 0.28% of yellow fever virus was determined. The distribution of pathogens responsible for diseases associated with yellow fever was: 49.47% (Plasmodium falciparum), 2% (hepatitis E virus), 4.62% (hepatitis C virus), and 29.00% (hepatitis virus B), respectively. Discrepancies in the results between the regional reference laboratories and the national laboratory of the Republic of Chad were observed. All genders and age groups were affected. Of the 1730 samples taken, 55.49% were female and 44.51% male (p = 0.01, a significant difference in favor of the female sex). The male/female sex ratio was 1.25. This study, the first, made it possible to determine the rate of the yellow fever virus in the absence of an outbreak in forest areas and with heavy rainfall and to evaluate the MAC-ELISA-CDC techniques used between the national laboratory of the Republic of Chad and those regional references. It was recommended that real-time polymerization chain reaction techniques be made available to national laboratories and reagents from the same manufacturing company in order to carry out effective monitoring of yellow fever. \u0000 \u0000 \u0000 \u0000 Key words: Evaluation, surveillance, seroprevalence, techniques, yellow fever.","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":"15 1","pages":"152-160"},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43445946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Antimicrobial activities of microbially-synthesized silver nanoparticles against selected clinical pathogens in Akure, Nigeria 尼日利亚阿库尔市微生物合成的银纳米颗粒对选定临床病原体的抗菌活性

African Journal of Microbiology Research Pub Date : 2021-03-31 DOI: 10.5897/AJMR2020.9401

E. Ekundayo, A. Adegbenro, F. Ekundayo, H. Onipede, O. Bello, I. A. Anuoluwa

{"title":"Antimicrobial activities of microbially-synthesized silver nanoparticles against selected clinical pathogens in Akure, Nigeria","authors":"E. Ekundayo, A. Adegbenro, F. Ekundayo, H. Onipede, O. Bello, I. A. Anuoluwa","doi":"10.5897/AJMR2020.9401","DOIUrl":"https://doi.org/10.5897/AJMR2020.9401","url":null,"abstract":"Silver nanoparticles (AgNPs) have stimulated interest of scientists due to their wide range of applications, including their potential antimicrobial activity. This study investigated the antimicrobial activities of AgNPs synthesized by rhizospheric soil and fish pond sediment microorganisms against selected clinical pathogens. The samples were cultured and organisms identified in accordance with standard procedures. The synthesis of AgNPs colloidal solution was monitored by UV-vis analysis. Presence of bands was determined by the Fourier Transform Infrared spectroscopy (FTIR). The antimicrobial activity of the synthesized AgNPs against selected clinical isolates was determined using agar well diffusion method. Ten species each of bacteria and fungi were isolated from the samples. Formation of AgNPs was indicated by colour transformation from yellow to brown. All synthesized AgNPs showed intense peak with wavelengths ranging of 410-440 nm in UV-vis. The FTIR revealed band at 3395 cm-1 and a strong peak at 3300-3500 cm-1. The AgNPs synthesized by some of the isolates exerted remarkable and varying degrees of antimicrobial activities against the susceptible test organisms. This study revealed that the microbially-synthesized AgNPs obtained from this study possess a high antimicrobial potency against most potential pathogens investigated, and, thus, can be exploited in the development of novel antimicrobial agents. \u0000 \u0000 \u0000 \u0000 Key words: Antimicrobial agents, microbial resistance, microorganisms, pathogens, silver nanoparticles, zones of inhibition.","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":"15 1","pages":"132-145"},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43602617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Degradation of lignocellulosic content of rice straw using aerobic cellulolytic bacteria isolated from forest soil of Bangladesh 用从孟加拉国森林土壤中分离的好氧纤维素分解菌降解稻草中的木质纤维素含量

African Journal of Microbiology Research Pub Date : 2021-03-31 DOI: 10.5897/AJMR2021.9498

M. Islam, M. Rahman, S. Islam, Shabbir Ahmed, M. Jahan, Sulav Indra Paul, A. Selim

{"title":"Degradation of lignocellulosic content of rice straw using aerobic cellulolytic bacteria isolated from forest soil of Bangladesh","authors":"M. Islam, M. Rahman, S. Islam, Shabbir Ahmed, M. Jahan, Sulav Indra Paul, A. Selim","doi":"10.5897/AJMR2021.9498","DOIUrl":"https://doi.org/10.5897/AJMR2021.9498","url":null,"abstract":"The present research work was conducted to enumerate and isolate aerobic cellulolytic bacteria from forest soil using carboxymethyl cellulose (CMC) as substrate. The identified isolate was further tested as potent candidate to improve the nutritional value of rice straw. The forest soil was loaded with substantial amount (2.45×107) of cellulolytic bacteria. A total of 10 cellulose degrading bacteria (CDB) were isolated, identified and monitored for their cellulolytic activity. The isolate having the highest cellulolytic index (2.5) was identified as Bacillus subtilis targeting the 16S rRNA gene which was labeled as B. subtilis strain CDB7 and investigated for nutritional improvement of rice straw. Solid state fermentation of each group was carried out at 37°C for a period of 0, 3 and 6 days in 0 (no bacterial inoculum), 1, 5 and 10% inoculum group. There were a tendency of reduction in Acid Detergent Fiber (ADF), Neutral Detergent Fiber (NDF) and lignin at concentrations of 1, 5 and 10% inoculums at every interval of fermentation. Highest reduction of ADF (4.8%), NDF (10.78%) and lignin (37.6%) were observed after 6 days of fermentation at 10, 10 and 5% inoculum group, respectively. The crude protein content was increased (5.3 ± 0.4 to 6.4± 0.3) in 10% inoculum group. Taken together, the identified isolate could be a potent candidate to degrade lignocellulosic content through breaking of lignin-cellulose bondage and to improve the nutritional value of rice straw. \u0000 \u0000 \u0000 \u0000 Key words: Rice straw, Bacillus subtilis strain CDB7, solid state fermentation, acid detergent fiber (ADF), neutral detergent fiber (NDF), lignin.","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":"15 1","pages":"161-170"},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49396196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapidly growing mycobacterial infections associated with plastic surgery: An epidemiological description 与整形手术相关的快速增长的分枝杆菌感染:流行病学描述

African Journal of Microbiology Research Pub Date : 2021-03-31 DOI: 10.5897/AJMR2020.9473

J. Braga, M. Prado, H. C. C. Neves, A. Tipple, D. M. Costa, S. L. A. Alves, HÃ©lio Galdino-JÃºnior, Katiane Martins MendonÃ§a, Silvana de Lima Vieira dos Santos

{"title":"Rapidly growing mycobacterial infections associated with plastic surgery: An epidemiological description","authors":"J. Braga, M. Prado, H. C. C. Neves, A. Tipple, D. M. Costa, S. L. A. Alves, HÃ©lio Galdino-JÃºnior, Katiane Martins MendonÃ§a, Silvana de Lima Vieira dos Santos","doi":"10.5897/AJMR2020.9473","DOIUrl":"https://doi.org/10.5897/AJMR2020.9473","url":null,"abstract":"Surgical site infections (SSI) caused by rapidly growing mycobacterial (RGM) have become increasingly frequent. The aim of this study is to describe the epidemiological, clinical aspects and factors associated with RGM infections related to plastic surgery. Notifications of SSI from 86 health care facilities of the capital of a Brazilian state within nine years, approximately, were assessed. RGM, predominantly M. fortuitum (39.0%), was isolated from 66 cases of infection folowing plastic surgery, mainly mammoplasty. All the cases were woman, with an average of 32.9 years. Amikacin/clarithomycin was the prevalent therapeutic regimen, and most of the isolates showed resistance to ciprofloxacin. There was an association of infection by M. abscessus and use of surgical instruments that were not exclusive to the institution (P=0.048). Thus, these findings emphasize the importance of SSI notifications and strict monitoring of surgical instruments reprocessing. \u0000 \u0000 \u0000 \u0000 Key words: Surgical site infection, plastic surgery, non-tuberculous mycobacteria.","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":"15 1","pages":"120-124"},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49020878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Identification of recombinants of hepatitis B virus genotypes C from Hong Kong China 中国香港地区乙型肝炎病毒基因型C重组体的鉴定

African Journal of Microbiology Research Pub Date : 2021-02-28 DOI: 10.5897/AJMR2020.9466

Qianqian Liu, Z. Tu

引用次数: 0

A study of bacterial pathogens associated with diarrhoea in children under 2 years in Ile-Ife, Nigeria 尼日利亚伊菲岛2岁以下儿童腹泻相关细菌病原体的研究

African Journal of Microbiology Research Pub Date : 2021-02-28 DOI: 10.5897/AJMR2020.9462

F. O. Akinwumi, O. Igbeneghu, O. Oyelami, A. Lamikanra

{"title":"A study of bacterial pathogens associated with diarrhoea in children under 2 years in Ile-Ife, Nigeria","authors":"F. O. Akinwumi, O. Igbeneghu, O. Oyelami, A. Lamikanra","doi":"10.5897/AJMR2020.9462","DOIUrl":"https://doi.org/10.5897/AJMR2020.9462","url":null,"abstract":"Diarrhoea, a major contributor to high mortality in children may be caused by an array of bacterial, viral or parasitic pathogens acting individually or synergistically. This study identified the bacterial pathogens associated with diarrhoea in children aged 0-24 months, established the frequency of diarrhoea caused by each pathogen encountered in diarrhoea and determined the antibiotic resistance pattern of the children's faecal isolates obtained to some commonly used antibiotics in the study environment. A total of 107 children within the age range 0-24 months presenting with diarrhoea and 115 apparently healthy children were recruited into this study. Stool samples or rectal swabs were collected and cultured using standard microbiological procedures. Recovered isolates were tested against commonly used antibiotics using disc diffusion method. The isolation of enteric pathogens was found to be significantly (p 0.05) difference in the isolates recovered from both diarrhoeal and control subjects. The isolates were found to be multiply resistant to all the antibiotics tested. However, the incidence of resistance to commonly-used antibiotics was found to be less than 50%. \u0000 \u0000 \u0000 \u0000 Key words: Diarrhoea, children, bacterial pathogens, antibiotic resistance, Ile-Ife.","PeriodicalId":7617,"journal":{"name":"African Journal of Microbiology Research","volume":"15 1","pages":"82-88"},"PeriodicalIF":0.0,"publicationDate":"2021-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46458478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1