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Microscopica acta最新文献

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Utilization of the histochemical techniques for the detection of various radicals of proteins and of 5-hydroxytryptamine in semi-thin sections. 利用组织化学技术在半薄切片中检测蛋白质和5-羟色胺的各种自由基。
Microscopica acta Pub Date : 1983-01-01
C Michel, D Damas, M Rusaouën
{"title":"Utilization of the histochemical techniques for the detection of various radicals of proteins and of 5-hydroxytryptamine in semi-thin sections.","authors":"C Michel, D Damas, M Rusaouën","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"87 1","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17462735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cytochemical mechanisms of chromatin fluorescence and staining reactions by thionin solutions. 染色质荧光和亚硫蛋白染色反应的细胞化学机制。
Microscopica acta Pub Date : 1983-01-01
M Cañete, R Armas-Portela, J C Stockert
{"title":"Cytochemical mechanisms of chromatin fluorescence and staining reactions by thionin solutions.","authors":"M Cañete,&nbsp;R Armas-Portela,&nbsp;J C Stockert","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>After staining with thionin at low concentration (less than 10(-5) M), the masses of condensed chromatin in chicken erythrocyte nuclei show a red fluorescence under green excitation, which is abolished when they become stained by using concentrations higher than 10(-4) M. The possibility that intercalative binding of a hydrophobic component from the dye solution into DNA accounts for this fluorescence reaction in chromatin is briefly discussed.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"87 1","pages":"35-8"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17252467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A microscope perfusion respirometer for continuous respiration measurement of cultured cells during microscopic observation. 显微镜灌注呼吸计，用于显微镜观察中培养细胞的连续呼吸测量。
Microscopica acta Pub Date : 1983-01-01
W K Schlage, J Bereiter-Hahn
{"title":"A microscope perfusion respirometer for continuous respiration measurement of cultured cells during microscopic observation.","authors":"W K Schlage,&nbsp;J Bereiter-Hahn","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The construction of a microscope perfusion respirometer (MPR) for simultaneous recording of cellular respiration and microscopic morphology is described. All light microscope techniques for living cells (e.g. phase contrast, differential interference contrast (DIC), fluorimetry) can be applied to the monolayer cells grown on a coverslip. The main constituents of the MPR are a) a precision operating perfusion pump (constant volume output), b) a modified Dvorak-Stotler perfusion chamber, c) a special holder for the Clark-type oxygen probe, d) gas-tight connections of stainless steel tubing with dead volume-free fittings, and e) a temperature control unit. The cell material, established XTH (Xenopus laevis tadpole heart) cell is characterized. Examples of operation are presented, concerning a) normal respiration, b) respiration during uncoupling of oxidative phosphorylation by carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP), and c) lactate production under anoxia. The corresponding mitochondrial in situ-morphology is demonstrated on photomicrographs. Details of construction and application are discussed. This new technique is supposed to extend the use of cell cultures instead of animal experiments in pharmaceutical routine tests.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"87 1","pages":"19-34"},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17880518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Carbodiimide and formalin as fixatives for the TP-levanol fast cyanine 5RN-method for staining myosin-like proteins in rat tissues. 碳二亚胺和福尔马林作为固定剂，用tp -左炔醇快速花青素5rn法对大鼠组织肌球蛋白样蛋白进行染色。
Microscopica acta Pub Date : 1982-11-01
E van Pelt-Verkuil
{"title":"Carbodiimide and formalin as fixatives for the TP-levanol fast cyanine 5RN-method for staining myosin-like proteins in rat tissues.","authors":"E van Pelt-Verkuil","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Apart from the applicability of the tannic acid-phosphomolybdic acid-Levanol-Fast Cyanine 5RN method for the identification of muscle myosins in Carnoy-fixed tissues, this technique can also be applied on tissue sections fixed with formalin or carbodiimide. The use of carbodiimide as a fixative offers, in general, a superior staining of the tissues with safranine and Levanol. Using a slightly modified procedure, reduction of staining time in Levanol, moreover, allows a differentiation between cells containing different amounts of myosin. This is illustrated by means of a number of rat tissues.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"86 4","pages":"323-38"},"PeriodicalIF":0.0,"publicationDate":"1982-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17250626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The behavior of antidiarrheal drugs in experiments]. [止泻药物在实验中的行为]。
Microscopica acta Pub Date : 1982-11-01
H H Janssen, W Queisser
{"title":"[The behavior of antidiarrheal drugs in experiments].","authors":"H H Janssen,&nbsp;W Queisser","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Four antidiarrheal drugs (China clay, bentonite, pectin, Kaoprompt H) are fed to mice by a gastric probe. Eight hours later, samples are taken from small intestine and colon to study the behaviour of the drugs in the gut. Any interactions with the brushborder of the villi at best are possible in the case of pectin. The viscosities of the suspended drugs depending on selected steps of pH are tested. The results are supporting the histological findings. The mechanical effects which may be put on the gut by the drugs are discussed.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"86 4","pages":"295-309"},"PeriodicalIF":0.0,"publicationDate":"1982-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18170814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A rapid method for the preparation of some Schiff-type dye-reagents for the demonstration of DNA: effect of UV rays. 一种快速制备用于演示DNA的希夫型染料试剂的方法:紫外线效应。
Microscopica acta Pub Date : 1982-11-01
M K Dutt
{"title":"A rapid method for the preparation of some Schiff-type dye-reagents for the demonstration of DNA: effect of UV rays.","authors":"M K Dutt","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"86 4","pages":"281-8"},"PeriodicalIF":0.0,"publicationDate":"1982-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17250624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rapid detection of pathognomonic blood cells in patients with infectious mononucleosis. 传染性单核细胞增多症患者病原性血细胞的快速检测。
Microscopica acta Pub Date : 1982-11-01
K Grossgebauer, H D Pohle
{"title":"Rapid detection of pathognomonic blood cells in patients with infectious mononucleosis.","authors":"K Grossgebauer,&nbsp;H D Pohle","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Veneous blood-citrate samples of patients with infectious mononucleosis were stained with DAPI, a newer fluorochrome (4',6-diamidino-2-phenylindole) to demonstrate the \"large atypical lymphocytes\" associated with this disease. As a result of the capacity of DAPI to stain DNA and certain acid mucopolysaccharides, a rapid detection of the pathognomonic white blood cells could be achieved. Most of them revealed the well known indented or lobulated nuclei and vacuolated (foamy) cytoplasm. Others exhibited kidney-shaped nuclei, often filled with yellow-fluorescent tiny granula. In our view, the rapid, specific and sensitive DAPI-technique for detecting pathognomonic blood cells in patients with infectious mononucleosis can be considered an improved microscopic method.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"86 4","pages":"289-93"},"PeriodicalIF":0.0,"publicationDate":"1982-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17250625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Morphometry of cortical neurons. The best estimation of perikaryon volume from the projection area. 皮质神经元形态学。从投影区域对核周体积的最佳估计。
Microscopica acta Pub Date : 1982-11-01
S Kühl, H Haug, W Schliesser
{"title":"Morphometry of cortical neurons. The best estimation of perikaryon volume from the projection area.","authors":"S Kühl,&nbsp;H Haug,&nbsp;W Schliesser","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The aim of this investigation is to develop the best approximation procedure for calculating the volumes of the perikarya of oriented cortical neurons. About 1119 nerve cells (area 6 after Brodmann) were identified. The medial cross section (projection area) and the maximal diameter of each cell was measured. The calculation is based on stereometrical models (sphere/cone/ellipsoid). The volume of a sphere of equal projection area has to be corrected for the actual shape of the cell type concerned (conical for pyramidal cells and ellipsoidal for granule and spindle cells). The correction factors are obtained by two different types of graphs. The procedure suggested depends on the available equipment. When working with systems which store only the projection area, the factor obtained for granule cells (0.9) should be used for all cells up to a projection area of 120 microns 2. Larger cells should be corrected by the factor derived for pyramidal cells (0.85). In connection with systems which provide the maximal diameter as well, the correction factors can be worked out by estimating the axial ratio of the neurons. Advanced systems like the Videoplan are able to calculate factors for individual cells.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"86 4","pages":"315-22"},"PeriodicalIF":0.0,"publicationDate":"1982-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18170815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
How many sections must be measured in order to reconstruct the volume of a structure using serial sections? 为了使用连续切片重建结构的体积，必须测量多少部分?
Microscopica acta Pub Date : 1982-11-01
K Zilles, A Schleicher, F W Pehlemann
{"title":"How many sections must be measured in order to reconstruct the volume of a structure using serial sections?","authors":"K Zilles,&nbsp;A Schleicher,&nbsp;F W Pehlemann","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>When reconstructing the volumes of organs, or other individual bodies, the areas of these structures are usually determined with the help of serial sections and the volume thus estimated. The actual number of serial sections that have to be taken into account, whilst arranging such a study, is of great importance. This present paper shows that about 20 equidistant sections are sufficient in order to determinate the volume, even that of extremely irregular formed bodies, whereby the error will not exceed 5%.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"86 4","pages":"339-46"},"PeriodicalIF":0.0,"publicationDate":"1982-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17812579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Immuno and enzyme histochemical demonstration of granulocytes--assay of a morphometric evaluation]. [粒细胞的免疫和酶组织化学证明——形态计量学评价的测定]。
Microscopica acta Pub Date : 1982-09-01
H Redl, H P Dinges
{"title":"[Immuno and enzyme histochemical demonstration of granulocytes--assay of a morphometric evaluation].","authors":"H Redl,&nbsp;H P Dinges","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Using the naphthol AS-D-chloroacetate esterase method and the indirect immunoperoxidase technique we tried to stain selectively granulocytes in the lung and in the liver. For the immuno technique we used an antibody raised in rabbits against dog granulocytes, which were prepared by a new separation technique. Comparing the two staining methods we found the immunoperoxidase to be superior at least for quantitative evaluation. In a pilot study with polytraumatized dogs versus controls we could detect a more pronounced leucostasis within the trauma group. This was similar to a parallel study which used radioactive labeled granulocytes where a significant increase could be shown. Granulocytes, at least in part, seem to be important for cellular changes within the \"lung in shock\".</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"86 3","pages":"207-14"},"PeriodicalIF":0.0,"publicationDate":"1982-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17808187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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