American Journal of Microbiological Research最新文献

{"title":"Diversity of the Bacterial Community of Three Soils Revealed by Illumina-Miseq Sequencing of 16S rRNA Gene in the South of Brazzaville, Congo","authors":"Shaloom Teresa Mabiala, J. Goma-Tchimbakala, Emerance Jessica Claire D’Assise Goma-Tchimbakala, A. A. Lebonguy, Alvychelle Benith Banga","doi":"10.12691/ajmr-8-4-4","DOIUrl":"https://doi.org/10.12691/ajmr-8-4-4","url":null,"abstract":"The study was conducted on three soils (MFILOU, SNR and ORSTOM) at the south of Brazzaville in Congo. The aim of the work was to study the composition and diversity of the soil microbial community. Microbial diversity was assessed using the Miseq-Sequencing. The results showed that microbial diversity was represented by 1289 OTUs assigned to the Bacteria (1282 OTUs), Fungi (2 OTUs), Viridiplantae (3 OTUs) and metazoa (2OTUs) domain with 97% of similarity. The 1282 bacterial OTUs were affiliated with 12 phyla, 31 classes, 59 orders, 122 families, 288 genera et 521 species for all sites. The Proteobacteria (45.59%-29.92%), Firmicutes (27.27%-7.35%), Acidobacteria (16.74%-10.15%), Actinobacteria (12.35%-5.07%) and Nitrospirae are the most abundant common phyla for the three sites. The most dominant common classes were Alphaproteobacteria (38.87%-24.77%), Bacilli (27.20%-6.95%), Acidobacteria (16.47%-7.59%), Actinobacteria (12.35%-5.07%) and Nitrospira (9.81%-1.78%). The most abundant common orders for the 3 sites are represented by Rhizobiales (33.96%-20.38%), Bacillales (27.20%-6.95%), Acidobacteriales (16.49%-7.61%), Actinomycetales (10.20%-3.6%) and Nitrospirales (9.81%-1.78%). Bacillaceae (25.37%-5.60%), Acidobacteriaceae (16.49%-7.61%), Bradyrhizobiaceae (10.81%-4.61%), Nitrospiraceae (9.81%-1.78%) and Chitinophagaceae (4.24%-1.59%) were the best distributed common families in the microbial community of the three sites. Bacillus (25.27%-5.56%), yhe most abundant and common genera were Rhodoplanes (15.48%-5.30%), Bradyrhizobium (10.74%-4.39%), Nitrospira (9.81%-1.78%) and Acidobacterium (8.49%-6.51%). At species level, Rhodoplanes spp. (15.26%-5.10%), Bradyrhizobium spp. (10.59%-4.35%) and Acidobacterium spp. (8.49%-6.51%) were the most common and abundant in the three soils.","PeriodicalId":7580,"journal":{"name":"American Journal of Microbiological Research","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84207749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, Molecular Detection and Antibiogram of Multi-drug Resistant Salmonella Typhimurium DT104 from Selected Dairy Farms in Mymensingh, Bangladesh","authors":"Shayka Tasnim Pritha, S. Rahman, Sadia Afrin Punom, Md. Mizanur Rahman, K. Nazir, M. Islam","doi":"10.12691/AJMR-8-4-3","DOIUrl":"https://doi.org/10.12691/AJMR-8-4-3","url":null,"abstract":"The Salmonella typhimurium DT104, an emerging cause of human illness has received an increasing attention due to its multidrug resistant properties. Since it has been isolated from human and other sources including food-producing animals around the world; it has become a worldwide public health concern. Therefore, the present study was designed to isolate, identify and study the antibiogram profile of multidrug resistant S. typhimurium DT104 from several dairy farms in Mymensingh district, Bangladesh. A total of 135 fecal samples from diarrhoeic cattle were collected aseptically and subjected for bacterial isolation, molecular detection using PCR followed by antibiogram study. Salmonella spp. could be isolated from a total of 39 (28.88%) samples based on cultural and staining methods which were further confirmed by PCR using invA gene specific primers. However, out of 39 Salmonella spp., 6 isolates were confirmed as S. typhimurium DT104 strain. Results of the antibiotic resistance patterns demonstrated that 100% (39/39) isolates were resistant to erythromycin followed by tetracycline (73.68%), colistin (89.47%), ampicillin (47.36%), gentamicin (21.05%), ciprofloxacin (31.57%), streptomycin (42.10%), enrofloxacin (10.52%) and chlormphenicol (31.57%). Moreover, about 23.07% isolates were resistant to more than 5 antibiotics. However, all the isolates were found to be sensitive to amikacin. These results suggest that antibiotic resistant S. typhimurium DT104 strain has been circulating in dairy cattle in Bangladesh which is alarming and may impose threat to livestock and public health due to lack of proper hygienic management. This study will be helpful for the selection of proper antibiotics against salmonellosis in cattle.","PeriodicalId":7580,"journal":{"name":"American Journal of Microbiological Research","volume":"22 1","pages":"136-140"},"PeriodicalIF":0.0,"publicationDate":"2020-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80632073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comprehensive Prospective Study to Understand Chikungunya Infection in Delhi Region during 2010-2011","authors":"Singh Pradeep Kumar, D. Lalit, Broor Shobha","doi":"10.12691/ajmr-8-4-2","DOIUrl":"https://doi.org/10.12691/ajmr-8-4-2","url":null,"abstract":"INTRODUCTION: Chikungunya is an arbovirus causing febrile illness with high strike rate and is known for outbreaks in more than 60 countries globally. During 2010-2011 unusual high number of suspected Chikungunya infection patients attended AIIMS OPD, Delhi, India. The NCR region with its semiarid geography restricts arbovirus outbreaks to monsoon months and is previously known for Dengue outbreaks although Chikungunya outbreaks were previously unknown to the region. The surge in suspected Chikungunya infection cases indicates a possible outbreak in the NCR region. This comprehensive prospective and follow-up study was designed to understand the Chikungunya infection among patients attending AIIMS OPD during 2010-11. METHOD: During June 2010 to Dec 2011, 2346 blood samples were collected from outpatients and inpatients attending AIIMS OPD, New Delhi. Samples were grouped in acute and chronic cases. Some patients were also enrolled for year-long follow-up study. Serum samples were tested for CHIKV using PCR and for IgM antibodies to chikungunya virus by IgM-capture ELISA. Real-time PCR was performed targeting the E1 gene for viral load determination in patient sera. RESULT: CHIKV positivity of 35% (746/2112) in acute and 67% (156/234) in chronic suspected cases were found by ELISA. most affected patients belonged to the age group >30-45 yrs. and above in both genders. Acute confirmed cases included 383 (51.3%) females and 363 (48.7%) males and chronic includes 92 (58.9%) females and 64 (41.1%) males. Clinical symptoms include polyarthralgia, fever, and rashes. Maximum positivity was seen in Oct-Nov of both years. During follow-up study, 118 patients enrolled, persisting polyarthralgia and anti-CHIKV IgM was detected up to 2 years while circulating CHIKV was detected by PCR up to 3 months in few patients. CONCLUSION: Chikungunya virus has emerged in Delhi during 2010 and contributed to about 30-40% of fever and arthralgia. CHIKV prevalence is highest in post monsoon month of October. The virus can remain in blood circulation for weeks, while anti-CHIKV IgM can persist for more than a year with complaints of periodic polyarthralgia.","PeriodicalId":7580,"journal":{"name":"American Journal of Microbiological Research","volume":"3 1","pages":"117-135"},"PeriodicalIF":0.0,"publicationDate":"2020-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73488296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Culture Conditions for Enhanced Bacteriocin Production by Lactococcus lactis MT186647 Using Response Surface Methodology","authors":"Onwuakor C.E, Ogbulie J.N., B. W., Ogbulie T.E., Nwokafor C.V., Uchendu C.E.","doi":"10.12691/AJMR-8-4-1","DOIUrl":"https://doi.org/10.12691/AJMR-8-4-1","url":null,"abstract":"Bacteriocins produced by various lactic acid bacteria (LAB) have found enormous use in the food industries as biopreservatives. This study evaluated the effect of varied culture conditions (temperature, pH, and NaCl concentration) on bacteriocin production by Lactococcus lactis MT186647 isolated from fermenting African oil bean seeds (Pentaclethra macrophylla Benth) using response surface methodology. A three-factor central composite design (CCD) was adopted with the interest of estimating the optimal conditions for its production using the response surface regression model, which evaluated the linear, squared, and interactive relationship between the response variables. The analysis of variance (ANOVA) using Minitab statistical software version 14.13 showed an R2 = 0.869 variations in the response variable for culture conditions. It was accounted for by the predictors suggesting that the model was adequate. The optimal culture condition for bacteriocin production by L. lactis was estimated at 30.5C, pH 5.9, 1.94% NaCl concentration at Y = 12.31 mm where Y represents the response (zone of inhibition) against Staphylococcus aureus ATCC 19095 using the agar well diffusion assay method. Validation of optimal values according to the regression model, produced an inhibition zone at Y = 13.33 ± 0.29 mm. There was a 19.33% increase in bacteriocin activity compared to an OFAT optimized medium from a previous study.","PeriodicalId":7580,"journal":{"name":"American Journal of Microbiological Research","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73228256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Secondary Metabolites Produced by Streptomyces on Uro-pathogens","authors":"S. A. Musa","doi":"10.12691/AJMR-8-3-4","DOIUrl":"https://doi.org/10.12691/AJMR-8-3-4","url":null,"abstract":"Soil streptomyces are known as a rich source to produce secondary metabolites. This study was conducted to examine the effect of the Streptomyces sp. extracts against the uro-pathogen isolates from urinary tract infections in pregnancy from Kassala state. Cultural, morphological and physiological characterization of 15 microorganisms isolates from four soil samples indicated that all isolates belonged to the Streptomyces genus. All Streptomyces isolates produced chitinase enzyme except isolate SU11. After 72 hours all of the isolates were produced L-asparaginase enzyme. Ethyl acetate extracts of Antimicrobial were used for secondary screening against uro-pathogen. The high activity has been observed against Gram-positive bacteria with a percentage of 73.3% when compared to gram-negative bacteria. Whilst 26.7% has a high activity against Gram-negative comparing with Gram-positive bacteria, and different activity was shown against candida spp.","PeriodicalId":7580,"journal":{"name":"American Journal of Microbiological Research","volume":"1996 1","pages":"103-109"},"PeriodicalIF":0.0,"publicationDate":"2020-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82743154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational Insights into the Drug Repurposing and Synergism of FDA-approved Influenza Drugs Binding with SARS-CoV-2 Protease against COVID-19","authors":"S. Parveen, R. Alnoman, A. Bayazeed, Alaa M. Alqahtani","doi":"10.12691/AJMR-8-3-3","DOIUrl":"https://doi.org/10.12691/AJMR-8-3-3","url":null,"abstract":"The concept of drug repurposing is extensively used currently to identify already approved/under investigation/discarded potential drugs for various other diseases, owing to the fact that many drugs could have multiple protein targets and many diseases share overlapping molecular pathways. The geographical spread of COVID-19 infections originating from Wuhan, China, has provided an opportunity to study the natural history of the recently emerged virus. The source of the SARS-CoV-2 is yet not known, even though the initial cases have been connected with the Huanan South China Seafood Market. In this study, the bioactivity of FDA-approved influenza drugs (Baloxavir, Oseltamivir, Peramivir and Zanamivir) were evaluated as inhibitors for COVID-19 using computational modeling approaches. The nominated drugs were docked on SARS-CoV-2 main protease (PDB ID: 6LU7) and also with SARS HCoV (PDB ID: 6NUR) for comparison, to evaluate the binding affinity of these drugs. ADMET and DFT analyses were also further carried out to analyze the potential of these influenza drugs as an effective inhibitor against COVID-19. The DFT calculations were performed to estimate the thermal parameters, dipole moment of the investigated drugs; additionally, chemical reactivity descriptors were investigated. The results of molecular docking with respect to binding energies in Kcal/mol suggested that binding affinity of influenza drugs with SARS-CoV-2 was in the order Zanamivir > Baloxavir > Oseltamivir > Peramivir. The findings of this study can facilitate rational drug design targeting the SARS-Cov-2 main protease.","PeriodicalId":7580,"journal":{"name":"American Journal of Microbiological Research","volume":"9 1","pages":"93-102"},"PeriodicalIF":0.0,"publicationDate":"2020-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81306237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Diversity of Plasmodium falciparum Isolated from Symptomatic and Asymptomatic Individuals in Parts of Kaduna Metropolis, Kaduna State, Nigeria","authors":"K. B. Dikwa, U. Yahaya, D. Maikaje, A. B. Suleiman","doi":"10.12691/AJMR-8-3-2","DOIUrl":"https://doi.org/10.12691/AJMR-8-3-2","url":null,"abstract":"Malaria due to Plasmodium falciparum is still a major public health problem and cause of high morbidity and mortality in Nigeria despite many efforts and interventions programmes put in place to control malaria, with transmission occurring throughout the year. Characterization of P. falciparum isolates from different geographical locations of Nigeria could provide the much needed information on the genetic composition of P. falciparum natural populations with regard to three polymorphic genes: Merozoite Surface Protein 1 (MSP 1), Merozoite Surface Protein (MSP 2) and Glutamate-Rich Protein (GLURP) genes, the findings of this study will assist in adopting more strategies and intervention programmes aim at controlling P. falciparum infection in Nigeria. This study was conducted to determine the genetic diversity and elucidate possible genomic variation between isolates of P. falciparum from symptomatic and asymptomatic malaria cases in Kaduna metropolis. Plasmodium falciparum genomic DNA was extracted from 42 positive blood samples collected onto whatmani¯s filter paper using the phenol-chloroform DNA extraction method. Genotyping of P. falciparum was based on the amplification of MSP 1, MSP 2 and glurp genes. Analysis of PCR products of P. falciparum isolates shows the presence of 33 alleles spread across MSP 1, MSP 2 and glurp genes at a frequency of 5, 24 and 4 respectively. Detection of the three genes; (MSP1); MAD20 and KI, (MSP2); FC27 and 3D7/ICI and (glurp) gene and the occurrence of single and multiple genotypic infection recorded showed the diverse genetic composition of P. falciparum population, an indication of the endemicity of malaria. This information is essential for effective malaria control programme.","PeriodicalId":7580,"journal":{"name":"American Journal of Microbiological Research","volume":"113 1","pages":"83-92"},"PeriodicalIF":0.0,"publicationDate":"2020-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74527541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Candida spp. Isolated from Urine Samples of Pregnant Women from Kassala State, Sudan","authors":"S. A. Musa, M. Magzoub, A. Alhassan, N. Hammad","doi":"10.12691/AJMR-8-3-1","DOIUrl":"https://doi.org/10.12691/AJMR-8-3-1","url":null,"abstract":"A urinary tract infection (UTI) is a major disease that affects people of all age groups. Women are more susceptible to UTI than men especially pregnancy, mainly due to the different structural features make easy contamination of the UTI with fecal flora. Candida is one of pathogenic which causing this problem. A total of 196 Clean-Catch midstream urine samples were collected from pregnancy referred to Kassala new Hospital and Abdurrahman Elmobark Medical center, their ages between 15 to 40 year old. Grams’ staining, chlamydospores formation and Chromogenic Agar medium used to differentiate between Candida spp. 17 (8.67%) were positive for Candida species, these were C. glabrata (41.18%), C. albicans (23.52%), C. krusei (17.66%), C. dubliniensis (11.76%) and C. tropicalis (5.88%). No co-existence of Candida species was found. In the present study C .glabrata and C. albicans scored the highest frequency of occurrence.","PeriodicalId":7580,"journal":{"name":"American Journal of Microbiological Research","volume":"96 1","pages":"79-82"},"PeriodicalIF":0.0,"publicationDate":"2020-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85676967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Identification of Fungal Pathogens Associated with Post-harvest Yam Tubers Rot in Mbam et Kim Division (Cameroon) with Emphasis on Penicillium monomenatosum (Frisvad, Filt. & Wicklow) as a First Report","authors":"Lile Christere Nguemnang Mabou, M. Sameza, S. Tchameni, P. Eke, R. Toghueo, A. Albertini, Pierre Gilbert Tamghe Gomsi, F. Boyom","doi":"10.12691/AJMR-8-2-5","DOIUrl":"https://doi.org/10.12691/AJMR-8-2-5","url":null,"abstract":"Nowadays, increasingly complains mention tremendous decay of stored yam tubers in the Mbam et Kim Division, the main producing zone of Cameroon. Knowledge of the specific fungi responsible for yam spoilage is paramount for proper preservation by implementing plausible control measures. This study was therefore undertaken to identify the causative fungal agents associated with yam tubers rot in the survey zone. The isolation of suspicious pathogens was performed as per the Koch’s postulates encompassing artificial inoculation, symptoms recording, re-isolation and comparisons-based re-identification. The identity of isolates was later on ascertained by the sequencing of the 5.8S ribosomal DNA gene (ITS1 and ITS2). Overall, seven fungal species: Fusarium solani, Fusarium oxysporum, Aspergillus niger, Penicillium monomenatosum, Rhizopus oryzea, Talaromyces flavovirens and Bionectria cf. ochroleuca were recovered. P. monomenatosum is reported here for the first time as causative agent of yam rot. The obtained results unveil the identity of the devastating fungus threatening yam availability to consumers throughout the year in Cameroon and represent an initial and crucial step toward planning efficient and reliable control measure to counter the yam rot epidemic.","PeriodicalId":7580,"journal":{"name":"American Journal of Microbiological Research","volume":"12 1","pages":"73-78"},"PeriodicalIF":0.0,"publicationDate":"2020-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80025526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactic Acid Bacteria and Yeasts in Spontaneously Fermented Sorghum Sourdough","authors":"Motunrayo O. Ewuoso, Oluwatoyin Habibat Animashaun, Adedapo A. Adejumo","doi":"10.12691/AJMR-8-2-4","DOIUrl":"https://doi.org/10.12691/AJMR-8-2-4","url":null,"abstract":"Introduction: Generally, natural fermentations are carried out by yeast and lactic acid bacteria forming a complex microbiota that acts in cooperation. Yeast have a prominent role in the production of beverages, due to the ability to accumulate high levels of ethanol and to produce highly desirable aroma compounds, but lactic acid bacteria are particularly important in fermentation because they produce desirable acids, flavor compounds, and peptides that inhibit the growth of undesirable organisms [1]. Studies on the ecology of sourdough microflora may help in the understanding of the microbial dynamics and differences between groups of closely related microbial population in cereal (sourdough) fermentations. In most natural fermentation, starters used are poorly known [2]. Methodology: Lactic acid bacteria and yeasts were isolated from Sourdoughsusing pour plating methods as described by Harrigan and McCance [3]. Mann Rogosa Sharpe (MRS) and potato dextrose medium were used for culturing lactic acid bacteria and yeasts respectively. Cultured MRS plates were incubated anaerobically at 30°C for 48h while that of PDA plates were incubated in an incubator at 25°C for 72h. The microbial populations of the sourdoughs were enumerated on each day of fermentation. Isolation and sub-culturing was done until pure cultures were obtained. Result: The spontaneously fermentative lactic acid bacteria and yeast populating the fermenting sorghum sourdough observed in this study increased rapidly as the fermentation time increased. The mutual or synergistic relationship between the duo confirmed and justified Wood (2004) report in fermenting food matrix. Wood and Hodge (1985), describe the co-existence of lactic acid bacteria and yeast in food processing as very crucial. Conclusion: The microbiological and physicochemical analysis of sorghum sourdough fermented spontaneously showed a synergistic relationship of lactic acid bacterial and yeast growing in it. The titratable acidity, pH, and temperature of the fermenting sorghum sourdough increased with increase days of fermentation with higher values observed in sorghum vulgare.","PeriodicalId":7580,"journal":{"name":"American Journal of Microbiological Research","volume":"7 1","pages":"63-72"},"PeriodicalIF":0.0,"publicationDate":"2020-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80964104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}