Dentistry in Japan最新文献

{"title":"Study of the Susceptibility of Antitumor Drug-Resistant Cells to LAK Cells","authors":"K. Hirata, Y. Sasakura, Usyaku Lee, J. Sindo","doi":"10.5794/JJOMS.44.913","DOIUrl":"https://doi.org/10.5794/JJOMS.44.913","url":null,"abstract":"It has been hypothesized that lymphokine-activated killer (LAK) cells may be effective against antitumor drug resistant (ATDR) squamous cell carcinoma. The objective of this study was to determine 1) the susceptibility of ATDR human tumor cell lines to interleukin-2 (IL-2)-induced LAK cell-mediated cytotoxicity, and to assess 2) the characteristics of the population of LAK cells as effector cells on the tumor cell lines tested and 3) the relationship between the susceptibility of ATDR cells and their expression of surface antigens.Parent strains of CEM (derived from leukemia), KB (derived from oral squamous cell carcinoma), and TCM (derived from metastasized lung cell carcinoma from oral squamous cell carcinoma) cells were used as drug-sensitive cells. The ATDR cell lines were established from the parent cells used; the ATDR cell lines were CEM (resistant to vinblastine, VLB; CEM/VLB0.1 and CEM/VLB1.0), KB (resistant to cisplatin, CDDP, and bleomycin, BLM; KB/CDDP1.0 and KB/BLM1.0, respectively) and TCM (resistant to CDDP; TCM/CDDP1.0). Peripheral blood lymphocytes were obtained from nine healthy volunteers and fractionated; the fractions consisted of unfractionated whole cells, CD 4 + cells, CD 8 + cells, and CD16+ cells. Each fraction was cultured with IL-2, and the induced LAK cells were used as effector cells.Whole-LAK cells were the most cytotoxic against all ATDR cell lines tested (as compared with the parent cells). However, the antitumor activity of the fractionated cells was limited (antitumor activity: CD16+-LAK, 60-80% on CEM/VLB0.1 and 1.0; CD 8+-LAK, about 30% on KB/CDDP1.0 and about 50% on TCM/CDDP1.0). The expressions of P-glycoprotein (Pgp), major histocompatibility complex (MHC) class I, intercellular adhesion molecule-1 (ICAM-1), and lymphocyte function-associated antigen-3 (LFA-3) were respectively high in CEM/VLB; KB/CDDP1.0, KB/BLM1.0 and TCM/CDDP1.0; KB/CDDP1.0 and KB/BLM1.0; and TCM/CDDP1.0. In contrast, the susceptibility of the ATDR cells to LAK killing was not affected by anti-P-gp, anti-MHC class I, anti-ICAM-1, or anti-LFA-3 monoclonal antibodies.These results indicate that the effective population of LAK cells varies according to the tumor cell line, and are consistent with the assumption that the susceptibility of drug resistant cells to LAK cells depends on the property of the parent tumor cells and the type of antitumor drug that induces ATDR cells.","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"36 1","pages":"73-76"},"PeriodicalIF":0.0,"publicationDate":"1998-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71043003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bactericidal Effect of Soft Alkalescence Electrolyzed Water on Periodontopathic Bacteria","authors":"T. Ogawa, Hirohiko Nakamura, H. Fujita, H. Kamoi, H. Nakaya, K. Kamoi","doi":"10.11263/JSOTP1982.17.81","DOIUrl":"https://doi.org/10.11263/JSOTP1982.17.81","url":null,"abstract":"An aqueous soft alkaline electrolyte solution is obtained by diaphramless electrolysis of water with a small amount of sodium chloride. Soft alkaline solution water included about 60 ppm effective chlorine that exhibits a bactericidal effect. In order to evaluate the possibility of using a soft alkaline solution water for periodontal treatment, the bactericidal effect of the solution on periodontopathic bacteria was investigated. Bactericidal effect of soft alkaline solution water was compared with hard oxidized water. The effect of saliva and serum and soft alkaline solution were compared to determine the bactericidal effect. The results revealed that soft alkaline solution water bactericidal effect is the same as hard oxidized water on all bacteria. Moreover, the bactericidal effect of soft alkaline solution water plus 10% saliva was stronger than hard oxidized water. The results suggested the usefulness of soft alkaline solution water in the periodontal treatment.","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"36 1","pages":"136-139"},"PeriodicalIF":0.0,"publicationDate":"1998-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63814639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum lgG Subclasses in Patients with Chronic Osteomyelitis of Mandible","authors":"H. Sakamoto, H. Naito, T. Aoki, K. Shiiki, J. Sasaki","doi":"10.11263/JSOTP1982.17.23","DOIUrl":"https://doi.org/10.11263/JSOTP1982.17.23","url":null,"abstract":"The relation between recurrent chronic osteomyelitis and IgG subclass deficiency has been suggested. In the present study, IgG subclasses concentrations in 7 patients with chronic mandibular osteomyelitis were investigated. Two patients with chronic therapy resistant osteomyelitis had a low level of the IgG3 subclass. Impaired host defense response could be a possible factor inducing chronic osteomyelitis in the mandihle.","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"36 1","pages":"133-135"},"PeriodicalIF":0.0,"publicationDate":"1998-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63814325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental Study on the Effects of Progesterone on the Fusion of the Fetal Mouse Palate in vitro","authors":"K. Ishii","doi":"10.5794/JJOMS.44.22","DOIUrl":"https://doi.org/10.5794/JJOMS.44.22","url":null,"abstract":"In the present study, the effects of progesterone on fusion of the secondary palate were examined in vitro. Methods: The maxillary region of A/J mouse fetuses (13.5 days) was dissected and cultured in a chemically-defined serum-free medium for 72 hours. Progesterone was added to the culture medium in a concentration of 0.1, 1.0, or 10ƒÊg/ml. Results: 1) Among 22 explants in the vehicle-control group, the palatal shelves completely fused in 10 (46%). The palatal shelves fused partially in 6 explants, contacted but did not fuse in 4 , and failed to contact in 2. 2) In the group treated with 0.1ƒÊg/ml progesterone (n=21), complete fusion occurred in 13 explants (62%) and partial fusion in 7 (33%). The palatal shelves contacted but did not fuse in 1 explant, and failed to contact in no explant. 3) When treated with 1.0ƒÊg/ml progesterone (n=22), the palatal shelves fused completely in 21 explants (95%) and partially in 1 (5%). None of the cultured palates failed to","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"35 1","pages":"68-72"},"PeriodicalIF":0.0,"publicationDate":"1998-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71043376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Tetracyclines on Chemotaxis of Human Periodontium Derived Fibroblasts","authors":"S. Deguchi, T. Hori","doi":"10.11263/JSOTP1982.16.1","DOIUrl":"https://doi.org/10.11263/JSOTP1982.16.1","url":null,"abstract":"","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"35 1","pages":"134-136"},"PeriodicalIF":0.0,"publicationDate":"1997-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63813176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Studies of Free Tissue Transfer in Oral and Maxillofacial Functional Reconstrucion - Comparative studies of vascularized and non-vascularized innervated forearm flaps for oral sensory restoration -","authors":"J. Sato, S. Yasumoto, K. Kawaguchi, Y. Hamada, H. Kitamura, K. Hayashi, I. Nakao, M. Matsuura, K. Seto","doi":"10.5794/JJOMS.41.690","DOIUrl":"https://doi.org/10.5794/JJOMS.41.690","url":null,"abstract":"Althogh a considerable number of flaps for reconstruction of the oral mucosa have been reported, each of these methods is associated with disadvantages such as loss of the sensations of touch. temperature, pain, and taste, the accumulation of food, a sensation of wearing dentures, loss of the sensations of mastication, and a strange feeling in the mouth. To overcome these problems, we developed a vascularized, innervated foream flap, which is elevated without removing the ramus communicans between the radial artery and vein and the vessels that nourish the lateral cutaneous nerves. We performed reconstruction of the tongue, gingiva, and mouth floor using vascularized innervated forearm flaps (three cases) and nonvascularized innervated forearm flaps (three cases). At 6 months postoperatively, sensation recovered slightly in one of three nonvascularized innervated forearm flaps, whereas the sensations of touch, pain and temperature recovered in all three vascularized innervated forearm flaps at 2 months postoperatively.","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"33 1","pages":"71-74"},"PeriodicalIF":0.0,"publicationDate":"1997-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71043462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mandibular Displacement during Clenching in the Intercuspal Position","authors":"M. Yanagida, Masahiro Tanaka, J. Tosa, T. Kawazoe","doi":"10.2186/JJPS.38.198","DOIUrl":"https://doi.org/10.2186/JJPS.38.198","url":null,"abstract":"Intercuspal position (ICP) is the most important position in all functional mandibular positions for the stomatognathic system. While the clenching level was gradually increased, dynamic stability of the ICP in 10normal subjects and 10patients with temporomandibular disorders (TMD) was evaluated from occlusal contacts, deviation of the incisal point of the mandible and bilateral anterior temporalis and masseter muscle activity.The obtained results were as follows:1. Increases in the clenching level produced changes in the location of occlusal contacts of ICP in TMD patients.2. During clenching of ICP, displacement of the mandible in TMD patients was greater than in normal subjects.3. During clenching of ICP, activity of the masseter muscle had a greater effect on the occlusal contact stability than the anterior temporalis muscle.","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"32 1","pages":"52-54"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68578902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infectious Lesion of Oral and Maxillofacial Region in Patient with Diabetes Mellitus","authors":"T. Nemoto, Y. Matsui, R. Yamamoto, R. Tsuchiya, K. Ohno, Michi Ken-ichi","doi":"10.11263/JSOTP1982.14.25","DOIUrl":"https://doi.org/10.11263/JSOTP1982.14.25","url":null,"abstract":"","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"33 1","pages":"134-138"},"PeriodicalIF":0.0,"publicationDate":"1995-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63811580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transient Bacteremia after Tooth Extraction","authors":"T. Morishima, J. Sasaki","doi":"10.11263/JSOTP1982.13.48","DOIUrl":"https://doi.org/10.11263/JSOTP1982.13.48","url":null,"abstract":"","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"32 1","pages":"131-132"},"PeriodicalIF":0.0,"publicationDate":"1994-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63810918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends in the Use of Antimicrobial Agents in Dental Practice Survey Among 29 Dental Colleges and University Hospitals in Japan","authors":"N. Yohkoh","doi":"10.11263/JSOTP1982.13.39","DOIUrl":"https://doi.org/10.11263/JSOTP1982.13.39","url":null,"abstract":"In an effort to survey antimicrobial agents actually used in dental practice, a multiinstitutional study was made of the consumption and share of antimicrobial agents as classified by chemi cal structure and dosage form. The study involved 29 dental colleges or university hospitals in Japan, and consisted of two parts: one, investigation of the share of antimicrobial agents used in a 10-day period from 28th September through 9th November, 1992, and the other, categorization of antimicrobial agents used over a 6-month period from April through September 1992. Antimicrobial agents accounted for 22% of all oral medicines, and ranked second just behind antiinflammatory and analgesic drugs which accounted for 27%. The order of share in descending frequency was: cephem series including Cefaclor and Cephalexin ; penicillin series such as Lenampicillin, Amoxycillin, and Bacampicillin. Injectable antimicrobial agents were used in as many as 62% of patients who were given injectable forms. The breakdown is as follows: cephem series, 50%; penicillin series, 27%; and others comprised of fosfomycin, aminoglyco sides and tetracycline series, only 14%.","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"32 1","pages":"128-130"},"PeriodicalIF":0.0,"publicationDate":"1994-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63811209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}