Dentistry in Japan最新文献

Effects of Platelet-released Supernatant on Mesenchymal Stem Cell Proliferation 血小板释放上清对间充质干细胞增殖的影响

Dentistry in Japan Pub Date : 2005-12-01 DOI: 10.11263/JSOTP1982.24.81

T. Matsuno, Takahiro Miyai, Gaku Tamazawa, Kazuki Kitahara, T. Miyasaka, Kazuhiko Omata, Chiaki Arai, T. Satoh

{"title":"Effects of Platelet-released Supernatant on Mesenchymal Stem Cell Proliferation","authors":"T. Matsuno, Takahiro Miyai, Gaku Tamazawa, Kazuki Kitahara, T. Miyasaka, Kazuhiko Omata, Chiaki Arai, T. Satoh","doi":"10.11263/JSOTP1982.24.81","DOIUrl":"https://doi.org/10.11263/JSOTP1982.24.81","url":null,"abstract":"Platelet-rich plasma (PRP), which is obtained by centrifuging autologous blood, contains a large number of platelets. When platelets are activated using substances such as thrombin, ƒ¿ granules release platelet released growth factor (PRGF) such as platelet-derived growth factor (PDGF) and transforming growth factor-ƒÀ (TGF-ƒÀ). Since these growth factors promote proliferation and differentiation of cells that are required for tissue regeneration, PRP has been used for bone and periodontal tissue regeneration in clinical dentistry. In the present study, we investigated the changes in PDGF-AB levels before and after platelet activation using ELISA. In addition, cellular proliferation was investigated by adding platelet released supernatant (PRS) produced by activating platelets of PRP to mesenchymal stem cell (MSC) culture media using MTS assay. The results showed that the level of PDGF-AB in PRS was 5.31 times higher than that before platelet activation. Furthermore, adding PRP or PRS significantly promoted MSC proliferation compared to the control, and adding PRS enhanced cellular proliferation in a dose-dependent manner from days 1 to 4 of culture. This study suggested that activation of platelets in PRP releases and concentrates PRGF, and that adding PRS to MSC promotes early cellular proliferation of MSC.","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"43 1","pages":"154-157"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63819284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of Heterozygosity and Microsatellite Instability on the Long Arm of Chromosome 2 in Human Oral Squamous Cell Carcinoma 人口腔鳞状细胞癌2号染色体长臂杂合性缺失和微卫星不稳定性

Dentistry in Japan Pub Date : 2005-08-20 DOI: 10.5794/JJOMS.51.374

Y. Kakimoto, H. Numasawa, N. Yamamoto, E. Takeda, T. Yamauchi, T. Shibahara

{"title":"Loss of Heterozygosity and Microsatellite Instability on the Long Arm of Chromosome 2 in Human Oral Squamous Cell Carcinoma","authors":"Y. Kakimoto, H. Numasawa, N. Yamamoto, E. Takeda, T. Yamauchi, T. Shibahara","doi":"10.5794/JJOMS.51.374","DOIUrl":"https://doi.org/10.5794/JJOMS.51.374","url":null,"abstract":"Frequent allelic imbalances, including loss of heterozygosity (LOH) and microsatellite instability (MSI), have been found on the long arm of chromosome 2 (2q) in several types of human cancer. This study was designed to identify the tumor suppressor locus (or loci) associated with oral squamous cell carcinoma (SCC) on 2q. To better understand the details of genetic alterations on 2q, we performed polymerase chainreaction analysis of microsatellite polymorphisms corresponding to 10 loci on this chromosome. We identified a novel tumor suppressor locus in this region in primary oral SCCs. To further determine the role of 2q deletions in oral carcinogenesis, 19 oral SCCs (19 sets of primary and corresponding normal tissues) were examined for allelic imbalances (LOH or MSI) on 2q, using ten microsatellite markers. Among these 19 patients, 11 (57.9%) showed LOH at one or more loci. Deletion mapping of these tumors revealed four discrete, commonly deleted regions on the chromosome arm. Furthermore, we detected MSI in 4 of the patients (21.1 %).We compared our results with clinicopathologic features. A number of sites with LOH on 2q were detected in early stage lesions, and the frequency of LOH was slightly but not significantly higher in later clinical stages. Our results suggest that allelic imbalances on 2q are involved in the development of oral SCC and that one or more putative tumor suppressor genes contributing to the pathogenesis of this disease are present on 2q.","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"43 1","pages":"70-73"},"PeriodicalIF":0.0,"publicationDate":"2005-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71043579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Enhanced Susceptibility to Fas-mediated Apoptosis by Interferon-γ in an Oral Squamous Cell Carcinoma Cell Line 干扰素-γ增强口腔鳞状细胞癌细胞对fas介导的凋亡的易感性

Dentistry in Japan Pub Date : 2004-11-20 DOI: 10.5794/JJOMS.50.629

T. Takemi, K. Takizawa, M. Iwase, M. Nagumo

{"title":"Enhanced Susceptibility to Fas-mediated Apoptosis by Interferon-γ in an Oral Squamous Cell Carcinoma Cell Line","authors":"T. Takemi, K. Takizawa, M. Iwase, M. Nagumo","doi":"10.5794/JJOMS.50.629","DOIUrl":"https://doi.org/10.5794/JJOMS.50.629","url":null,"abstract":"Fas is widely expressed on the cell surface of many normal and neoplastic cells and induces apoptotic cell death in the presence of Fas ligand (FasL) or Fas-agonistic antibody CH11 (Fas-mediated apoptosis) Cancer cells constitutively expressing Fas are generally resistant to Fas-mediatedapoptosis. Recently, it has been reported that interferon-ƒÁ (IFN-ƒÁ) enhances Fas-mediated apoptosis. An oral squamous cell carcinoma cell line, NA, also constitutively expresses Fas on the plasma membrane and shows low susceptibility to Fas-agonistic antibody-induced apoptosis. This study was conducted to examine the effects of IFN-ƒÁy on Fas-mediated apoptosis, the expression of Fas and FasL, and the production of soluble Fas (sFas) in NA cells. The results revealed that a Fas-agonistic antibody, CH11, induced apoptosis of NA cells and IFN-ƒÁ enhanced susceptibility of NA cells to CH11-induced apoptosis. Further more, IFN-ƒÁ up-regulated Fas expression on NA cells without affecting the expression of FasL. A slight decrease in sFas expression was induced by treatment with IFN-ƒÁ. These results suggest that IFN-ƒÁ may enhance the susceptibility of NA cells to Fas-mediated apoptosis through the up-regulation of Fas.","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"42 1","pages":"69-72"},"PeriodicalIF":0.0,"publicationDate":"2004-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71043556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Biological Investigation in Candida Albicans Isolated from Recurrent Candidiasis of the Tongue 舌念珠菌复发性念珠菌分离的白色念珠菌分子生物学研究

Dentistry in Japan Pub Date : 2004-08-01 DOI: 10.11263/JSOTP1982.23.54

Isao Hasegawa, T. Satoh, I. Kobayashi

引用次数: 0

Effect of Calcium Channel Blockers on Proliferation of Human Gingival Fibroblast (Gin-1) 钙通道阻滞剂对人牙龈成纤维细胞Gin-1增殖的影响

Dentistry in Japan Pub Date : 2004-04-01 DOI: 10.11263/JSOTP1982.23.1

Hidetoshi Ito, K. Tsuchikawa, H. Katsuragi, Kazuko Saito

引用次数: 1

Maxillofacial Bone Regeneration Using Tissue Engineering Concepts 利用组织工程概念进行颌面骨再生

Dentistry in Japan Pub Date : 2003-03-01 DOI: 10.1201/9781498713535-22

M. Ueda

引用次数: 5

Characteristics of Osteogenesis Around Dental Implants Inserted into Vascularized Bone Grafts and Free Bone Grafts in Mandible of Dogs 犬下颌骨血管化骨移植和游离骨移植牙种植体周围成骨的特点

Dentistry in Japan Pub Date : 2002-11-20 DOI: 10.5794/JJOMS.48.557

Yoshitaka Furuya, Y. Yajima, H. Noma

{"title":"Characteristics of Osteogenesis Around Dental Implants Inserted into Vascularized Bone Grafts and Free Bone Grafts in Mandible of Dogs","authors":"Yoshitaka Furuya, Y. Yajima, H. Noma","doi":"10.5794/JJOMS.48.557","DOIUrl":"https://doi.org/10.5794/JJOMS.48.557","url":null,"abstract":"The objective of this study was to investigate the characteristics of osteogenesis around dental implants in both vascularized and nonvascularized bone grafts. Vascularized bone grafts were nourished by inferior alveolar vessels providing medullary blood flow to the mandibles of adult dogs. Titanium implants were placed into the bone grafts and normal mandibles. The time course of osteogenesis around the implants was observed histologically from 2 to 24 weeks after operation. Specimers stained with Stevenel's blue and Van Gieson-picrofuchsin stains and fluorescently labeled specimens were examined with a confocal laser scanning microscope. Implant-bone contact rates were determined.In vascularized bone grafts, new bone formation was evident all around the implants at 2 weeks postoperatively. New bone became compact with the passage of time. These processes of new bone formation were similar to those of normal mandibles.In cortical bone of vascularized bone grafts, bone resorption was recognized. However, bone remodeling was found at an early stage, suggesting that implants could be placed into vascularized bone grafts immediately after the primary operation.In nonvascularized bone grafts, bone resorption and remodeling occurred from the outside of cortical bone. New bone formation around implants was found at 8 weeks postoperatively. The implant apex was encapsulated by fibrous tissue at 24 weeks postoperatively. The results suggest that simultaneous implantation with free bone grafting requires a long time for bony linkage and that encapsulation of implants by fibrous tissue may occur in nonvascularized bone grafts.","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"40 1","pages":"63-66"},"PeriodicalIF":0.0,"publicationDate":"2002-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71043164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Prognostic Factors for Survival According to Treatment Period in Oral Squamous Cell Carcinomas 口腔鳞状细胞癌治疗期的预后因素分析

Dentistry in Japan Pub Date : 2001-06-20 DOI: 10.5794/JJOMS.47.331

Y. Kido, M. Noguchi, H. Kinjyo, Tatsuru Suyama, H. Kubota, A. Miyazaki, G. Kohama

{"title":"Prognostic Factors for Survival According to Treatment Period in Oral Squamous Cell Carcinomas","authors":"Y. Kido, M. Noguchi, H. Kinjyo, Tatsuru Suyama, H. Kubota, A. Miyazaki, G. Kohama","doi":"10.5794/JJOMS.47.331","DOIUrl":"https://doi.org/10.5794/JJOMS.47.331","url":null,"abstract":"The purpose of this study was to assess changes in prognostic factors related to modification of surgical treatment on the basis of grade of tumor malignancy and to contribute to improved surgical treatment of patients with oral squamous cell carcinoma. Fourhundred three patients with oral squamous cell carcinoma treated by surgery were classified according to treatment period as follows: f irst period, 1976-1986; second period, 1987-1991; and third period, 1992-1997.Three and 5-year cumulative disease-specific survival rates according to treatment period were as follows: first period (n=187), 78.6, 76.3%; second period (n=113), 76.6, 73.7%; and third period (n=103), 88.9, 87.2%. On univariate analysis of survival according to T, N, stage, growth pattern, and mode of invasion, significant differences (P<0.05) were found for all characteristics in the first and second periods, while in the third period no significant difference was found for growth pattern or mode of invasion.Cox regression analysis of disease-specific survival identified the following as independent prognostic factors: mode of invasion (1+2, 3, 4C+4D), growth pattern (exophytic, endophytic), and N (0, 1, 2) in the first period, mode of invasion (1-3, 4C+4D) and N (0, 1, 2+3) in the second period, and only N (0, 1, 2) in the third period.Owing to the improved outcome for highly invasive carcinoma, the prognostic values during the third period differed from those during the first and second periods.","PeriodicalId":75798,"journal":{"name":"Dentistry in Japan","volume":"39 1","pages":"76-79"},"PeriodicalIF":0.0,"publicationDate":"2001-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71043098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Loss of Heterozygosity on the Short Arm of Chromosome 3 in Oral Squamous Cell Carcinomas : Relationship between loss of heterozygosity on 3p25-ter region and clinical and histological features 口腔鳞状细胞癌3号染色体短臂杂合性缺失:3p25-ter区域杂合性缺失与临床和组织学特征的关系

Dentistry in Japan Pub Date : 2000-08-20 DOI: 10.5794/JJOMS.46.455

N. Uzawa, D. Akanuma, A. Negishi, T. Amagasa, Mitsuaki Yoshida

引用次数: 5

Effects of Antibiotics on Phagocytosis of Polymorphonuclear Leukocytes in Experimental Rabbit Infection Models 抗生素对兔感染模型中多形核白细胞吞噬作用的影响

Dentistry in Japan Pub Date : 1999-04-01 DOI: 10.11263/JSOTP1982.19.28

Kazuki Kitahara, T. Satoh

引用次数: 1