Current topics in molecular endocrinology最新文献_第8页

Androgen binding in the testis: in vitro production of androgen binding protein (ABP) by Sertoli cell cultures and measurement of nuclear bound androgen by a nuclear exchange assay. 睾丸中的雄激素结合:通过支持细胞培养体外产生雄激素结合蛋白(ABP)和通过核交换试验测量核结合雄激素。

Current topics in molecular endocrinology Pub Date : 1975-01-01 DOI: 10.1007/978-1-4613-4440-7_21

B M Sanborn, J S Elkington, A Steinberger, E Steinberger

{"title":"Androgen binding in the testis: in vitro production of androgen binding protein (ABP) by Sertoli cell cultures and measurement of nuclear bound androgen by a nuclear exchange assay.","authors":"B M Sanborn, J S Elkington, A Steinberger, E Steinberger","doi":"10.1007/978-1-4613-4440-7_21","DOIUrl":"https://doi.org/10.1007/978-1-4613-4440-7_21","url":null,"abstract":"Androgen binding activity in the testis has two components. One component, ABP, has been shown to be produced by Sertoli cell cultures for at least 9 days in the absence of exogenously added hormones. FSH (10-100 microgram/ml) markedly enhances the secretion of ABP. MIX has a potentiating effect after long treatment intervals (7 days). In order to study the second component, intracellular androgen receptor, a nuclear exchange assay was developed. Competition for exchange activity using 3H-dihydrotestosterone was significant for a 500 fold excess of testosterone, dihydrotestosteron, progesterone, and cyproterone acetate. The exchange activity was increased 2-10 fold by prior treatment in vitro or in vivo with testosterone. Significant exchange activity was found in long-term hypophysectomized adult and immature animals and in tubule and germ cell fractions. In isolated germ cell fractions, the highest concentration of exchange activity was associated with the most mature elements. These data suggest that androgen exchange activity may exist in both Sertoli cell and germ cell fractions and suggest that the mechanism of action of androgens in the testis is quite complex.","PeriodicalId":75766,"journal":{"name":"Current topics in molecular endocrinology","volume":"2 ","pages":"293-309"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-4613-4440-7_21","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12412692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 39

Transcription during mammalian spermatogenesis with special reference to Sertoli cells. 哺乳动物精子发生过程中的转录，特别涉及支持细胞。

Current topics in molecular endocrinology Pub Date : 1975-01-01 DOI: 10.1007/978-1-4613-4440-7_32

L L Tres, A L Kierszenbaum

引用次数: 4

Gonadotropic regulation of 5 alpha-reductase activity in the interstitial cells and whole testis homogenate of the immature rat. 促性腺激素对未成熟大鼠间质细胞和全睾丸匀浆中5 - α还原酶活性的调节。

Current topics in molecular endocrinology Pub Date : 1975-01-01 DOI: 10.1007/978-1-4613-4440-7_4

S N Nayfeh, J C Coffey, N J Kotite, F S French

引用次数: 10

Synthesis of estradiol-17 beta by Sertoli cells in culture: stimulation by FSH and dibutyryl cyclic AMP. 培养的支持细胞合成雌二醇-17 β: FSH和二丁基环AMP的刺激。

Current topics in molecular endocrinology Pub Date : 1975-01-01 DOI: 10.1007/978-1-4613-4440-7_6

D T Armstrong, Y S Moon, I B Fritz, J H Dorrington

{"title":"Synthesis of estradiol-17 beta by Sertoli cells in culture: stimulation by FSH and dibutyryl cyclic AMP.","authors":"D T Armstrong, Y S Moon, I B Fritz, J H Dorrington","doi":"10.1007/978-1-4613-4440-7_6","DOIUrl":"https://doi.org/10.1007/978-1-4613-4440-7_6","url":null,"abstract":"Sertoli cells isolated from the testes of 18 to 20-day old rats synthesize estradiol-17 beta when grown in primary culture in the presence of testosterone (5 X 10(-7) M). After an initial lag phase of about 2 hours, follicle-stimulating hormone (FSH) stimulates synthesis of estradiol up to 50-fold, synthesis being approximately linear for 24 hours when medium is changed every 2-6 hours. Luteinizing hormone (LH) causes only a marginal stimulation, at concentrations consistent with contamination with FSH. Dibutyryl cyclic 3',5'-adenosine monophosphate (dbcAMP) (10(-4)M) mimicks the effect of FSH. No significant synthesis occurs in the absence of steroid substrate or in the presence of pregnenolone (5 X 10(-7) M). When cells are pre-incubated for 24 hours with FSH in the absence of testosterone, addition of testosterone results in an immediated increase in estradiol synthesis without a lag phase, suggesting that FSH or dbcAMP are capable of inducing the conditions necessary for stimulation in the absence of testosterone. These observations provide the first direct evidence of estradiol-17 beta synthesis by Sertoli cells from normal animals, and offer evidence that synthesis of this steroid is regulated at the level of the aromatizing enzyme system by FSH and cyclic AMP.","PeriodicalId":75766,"journal":{"name":"Current topics in molecular endocrinology","volume":"2 ","pages":"85-96"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11412464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Physicochemical and biological properties of androgen receptors. 雄激素受体的物理化学和生物学特性。

Current topics in molecular endocrinology Pub Date : 1975-01-01 DOI: 10.1007/978-1-4613-4440-7_17

C W Bardin, O Jänne, L P Bullock, S T Jacob

引用次数: 8

Androgen transport and receptor mechnisms in testis and epididymis. 睾丸和附睾雄激素转运和受体机制。

Current topics in molecular endocrinology Pub Date : 1974-01-01 DOI: 10.1007/978-1-4684-2595-6_16

F S French, W S McLean, A A Smith, D J Tindall, S C Weddington, P Petrusz, M Sar, W E Stumpf, S N Nayfeh

引用次数: 4

Conformational and metabolic aspects of gonadotropins. 促性腺激素的构象和代谢方面。

Current topics in molecular endocrinology Pub Date : 1974-01-01 DOI: 10.1007/978-1-4684-2595-6_6

D Puett, M Ascoli, L A Holladay

引用次数: 11

Testicular estradiol receptors in the rat. 大鼠睾丸雌二醇受体。

Current topics in molecular endocrinology Pub Date : 1974-01-01 DOI: 10.1007/978-1-4684-2595-6_21

E Mulder, W M van Beurden-Lamers, W De Boer, A O Brinkman, H J van der Molen

引用次数: 21

Effects of FSH on levels of androgen binding protein in the testis. 卵泡刺激素对睾丸雄激素结合蛋白水平的影响。

Current topics in molecular endocrinology Pub Date : 1974-01-01 DOI: 10.1007/978-1-4684-2595-6_19

I B Fritz, B Kopec, K Lam, R G Vernon

引用次数: 42

FSH binding in rat testes during maturation and following hypophysectomy. Cellular localization of FSH receptors. 成熟和垂体切除术后大鼠睾丸中FSH的结合。FSH受体的细胞定位。

Current topics in molecular endocrinology Pub Date : 1974-01-01 DOI: 10.1007/978-1-4684-2595-6_10

A Steinberger, K H Thanki, B Siegal

引用次数: 52