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Bollettino dell'Istituto sieroterapico milanese最新文献

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[Seroepidemiologic study of human leptospirosis in Lombardy]. 伦巴第地区人类钩端螺旋体病的血清流行病学研究。
Bollettino dell'Istituto sieroterapico milanese Pub Date : 1989-01-01
L Gelosa, A Perone
{"title":"[Seroepidemiologic study of human leptospirosis in Lombardy].","authors":"L Gelosa,&nbsp;A Perone","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In 1987 a seroepidemiological research was performed to verify the prevalence of leptospira agglutinins on the urban and rural population of Milan, Bergamo and Mantova Provinces in Lombardia Region. Were controlled 150 subjects (75 males and 15 females) in Milan town, 58 (49 males and 9 females) in municipalities of Bergamo Province, and 68 (55 males and 13 females) of Mantova Province. La prevalence of leptospira agglutinins in exposed subjects was 14.3% and in unexposed subjects 8.6%. In the females was noted a significative higher prevalence of agglutinins (14.4%) than in males (9.5%), especially in the females exposed (36.3%) than in females unexposed (9.6%). The leptospira serovars most interested were L. sejroe (15.9%), L. poi (13.6%), L. bratislava, L. canicola and L. Zanoni (9.1%). The sera tested proved reactivity of 56.8% also against two or three different serovars of pathogenic leptospira and prevalence of 13.6% of coagglutinins against nonpathogenic L. patoc serovar.</p>","PeriodicalId":75613,"journal":{"name":"Bollettino dell'Istituto sieroterapico milanese","volume":"68 2","pages":"127-41"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13637828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
HIV infection in children born to seropositive mothers: a laboratory and clinical follow-up study. 血清阳性母亲所生儿童的HIV感染:一项实验室和临床随访研究。
Bollettino dell'Istituto sieroterapico milanese Pub Date : 1989-01-01
B De Rienzo, V Borghi, I Cocchi, G Frigeri, G B Cavazzuti, M Pecorari, P Pietrosemoli, G Lami, F Squadrini
{"title":"HIV infection in children born to seropositive mothers: a laboratory and clinical follow-up study.","authors":"B De Rienzo,&nbsp;V Borghi,&nbsp;I Cocchi,&nbsp;G Frigeri,&nbsp;G B Cavazzuti,&nbsp;M Pecorari,&nbsp;P Pietrosemoli,&nbsp;G Lami,&nbsp;F Squadrini","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Several serological techniques may be used for the detection of HIV infection, however, in the case of infants, there are no methods that are effective prior to the loss of maternal antibodies. Thus, in such cases, a completed series of serological and virological assay of HIV antibodies should be performed in follow-up studies. Twenty children living in Modena (Italy) were studied since 1985. Seven of these children were of ages ranging from 3 to 10 years. Only one of them was found to be seropositive for HIV. The remaining 13 children were studied from birth. Six had lost maternal antibodies to HIV at a median age of 15.1 months. Four showed decreasing levels of the anti-body by Western blotting. HIV antigen was detected in serum samples from 2 children. The data reported stress the need for longitudinal studies that could lead to reliable methods of diagnosing HIV infections in newborns.</p>","PeriodicalId":75613,"journal":{"name":"Bollettino dell'Istituto sieroterapico milanese","volume":"68 2","pages":"180-4"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13637833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Viral hepatitis b. Immunology and pathogenesis]. 病毒性乙型肝炎。免疫学和发病机制。
Bollettino dell'Istituto sieroterapico milanese Pub Date : 1989-01-01
G Cristina, M U Mondelli
{"title":"[Viral hepatitis b. Immunology and pathogenesis].","authors":"G Cristina,&nbsp;M U Mondelli","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75613,"journal":{"name":"Bollettino dell'Istituto sieroterapico milanese","volume":"68 3","pages":"289-95"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13638221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Carriers of enterotoxic Staphylococcus aureus during a 2 month period in Sienese workers]. [锡耶纳工人2个月期间肠毒性金黄色葡萄球菌携带者]。
Bollettino dell'Istituto sieroterapico milanese Pub Date : 1989-01-01
M Zanda, A D'Errico, E Quartesan, T Pozzi, M Cozzani
{"title":"[Carriers of enterotoxic Staphylococcus aureus during a 2 month period in Sienese workers].","authors":"M Zanda,&nbsp;A D'Errico,&nbsp;E Quartesan,&nbsp;T Pozzi,&nbsp;M Cozzani","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>From the period of October 1987 to January 1988, 9 samples were taken from 16 workers in company canteens situated in the Sienna area. The study of enterotoxin staphylococci strains was carried out of the pharynx, nose, skin of the face and the hands. The investigation required the use of the Staphylo-Zyme P.B.I. kit and RPLA Oxoid set. The most frequently found enterotoxins were A and D, either alone or together.</p>","PeriodicalId":75613,"journal":{"name":"Bollettino dell'Istituto sieroterapico milanese","volume":"68 3","pages":"296-9"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13638222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Post-transfusional non-A non-B hepatitis. Evaluation of SGPT screening in blood donors]. [输血后非a非b型肝炎。献血者SGPT筛查的评价[j]。
Bollettino dell'Istituto sieroterapico milanese Pub Date : 1989-01-01
M Muglia, U Angeloni, P Angeloni
{"title":"[Post-transfusional non-A non-B hepatitis. Evaluation of SGPT screening in blood donors].","authors":"M Muglia,&nbsp;U Angeloni,&nbsp;P Angeloni","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The incidence of non-A, non-B hepatitis is 10% of post-transfusion hepatitis (PTH), and more than 30% of this occurrence could be prevented with SGPT screening. We have been studying from 1984 this surrogate test in order to prevent non-A, non-B post-transfusion hepatitis. Reported data suggest this screening in all blood donors.</p>","PeriodicalId":75613,"journal":{"name":"Bollettino dell'Istituto sieroterapico milanese","volume":"68 1","pages":"28-33"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13664202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cellular and humoral modifications during response to HBsAg vaccine in healthy subjects: comparison between French and American vaccines. 健康受试者对乙肝表面抗原疫苗反应期间的细胞和体液修饰:法国和美国疫苗的比较
Bollettino dell'Istituto sieroterapico milanese Pub Date : 1989-01-01
R D'Amelio, P M Matricardi, A Di Addario, S Le Moli, E Paganelli, R Nisini, M C Sirianni, A Fattorossi, M Cherchi, C Gaetano
{"title":"Cellular and humoral modifications during response to HBsAg vaccine in healthy subjects: comparison between French and American vaccines.","authors":"R D'Amelio,&nbsp;P M Matricardi,&nbsp;A Di Addario,&nbsp;S Le Moli,&nbsp;E Paganelli,&nbsp;R Nisini,&nbsp;M C Sirianni,&nbsp;A Fattorossi,&nbsp;M Cherchi,&nbsp;C Gaetano","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Two groups of 18 healthy adults, chosen from a risk population, were vaccinated against hepatitis B virus with French (Hevac B, Pasteur) or American (HB-VAX, MSD) vaccine; immunological and biochemical parameters (HBsAb titers, Ig, IgM-rheumatoid factor (IgM-RF) levels, autologous mixed lymphocyte reaction (AMLR), T4/T8 ratio, lymphocyte migration inhibition test (LMIT) with specific antigen and transaminase levels) were compared at preselected time intervals. The vaccines were found to be clinically safe, as documented by the absence of demonstrable side effects and changes in serum transaminase levels. The positivity of the LMIT test demonstrated an early immunization on day 45 in all subjects tested, along with a marked reduction in AMLR, independent of the vaccine used. A slightly higher, though not statistically significant, percentage of seroconversion was observed for the Pasteur vaccine. This same preparation induced a significant increase in the main Ig classes and in Ig;-RF levels on day 45. This data indicates that aspecific modification of the immune system occur to a greater extent with Hevac B, presumably for its lower degree of purification. Thus, despite the slightly lower efficacy, the HB-VAX might be the preparation of choice in subjects with immunological disturbances.</p>","PeriodicalId":75613,"journal":{"name":"Bollettino dell'Istituto sieroterapico milanese","volume":"68 1","pages":"57-61"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13679345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Technical factors influencing the bactericidal activity of teicoplanin against staphylococci. 影响替柯planin对葡萄球菌杀菌活性的工艺因素。
Bollettino dell'Istituto sieroterapico milanese Pub Date : 1989-01-01
M B Pasticci, A Moretti, R F Frongillo, F Baldelli, M V Moretti, S Pauluzzi
{"title":"Technical factors influencing the bactericidal activity of teicoplanin against staphylococci.","authors":"M B Pasticci,&nbsp;A Moretti,&nbsp;R F Frongillo,&nbsp;F Baldelli,&nbsp;M V Moretti,&nbsp;S Pauluzzi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Considering 99.9% Killing after 24h of incubation, mean bactericidal activity of teicoplanin against 48 clinical isolates of Staphylococci resulted to be 67.7 micrograms/ml. Fortyfour out of 48 strains (91.7%) resulted tolerant. The high percentage of tolerant was dependent on survivors above the 0.1% allowed in several tube dilutions. Prolonged incubation consistently reduced this number. Only 37.5% of our strains remained tolerant after 48h of incubation. Bactericidal activity of teicoplanin was also significantly influenced by other technical factors. Human serum enhanced the killing of teicoplanin. For 9 strains of Staphylococcus aureus geometric mean MBC decreased from 49.9 muug/ml to 3.9 micrograms/ml (p less than 0.05); also actively growing bacteria demonstrated a higher killing rate, resulting in lower MBCs (mean 3.8 micrograms/ml). Micromethod technique was greatly influenced by the carryover phenomenon. The spot subculture technique is not accurate for bactericidal studies. A standardized technique for determining the minimal bactericidal activity is needed.</p>","PeriodicalId":75613,"journal":{"name":"Bollettino dell'Istituto sieroterapico milanese","volume":"68 2","pages":"101-7"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13679346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The use of single locus and multilocus minisatellite probes to diagnose specific individuality. 使用单位点和多位点小卫星探针诊断特异性个体化。
Bollettino dell'Istituto sieroterapico milanese Pub Date : 1989-01-01
M Esposito, G Ciliberto
{"title":"The use of single locus and multilocus minisatellite probes to diagnose specific individuality.","authors":"M Esposito,&nbsp;G Ciliberto","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75613,"journal":{"name":"Bollettino dell'Istituto sieroterapico milanese","volume":"68 3","pages":"214-23"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13719606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[In vitro sensitivity of 3 strains of Leptospira interrogans to 3 different antibiotics]. [3株疑问钩端螺旋体对3种不同抗生素的体外敏感性]。
Bollettino dell'Istituto sieroterapico milanese Pub Date : 1989-01-01
R Benzi Cipelli, G Gorini, S Caligaris, P Lanzarini, F Milano, P Orsolini, G Filice
{"title":"[In vitro sensitivity of 3 strains of Leptospira interrogans to 3 different antibiotics].","authors":"R Benzi Cipelli,&nbsp;G Gorini,&nbsp;S Caligaris,&nbsp;P Lanzarini,&nbsp;F Milano,&nbsp;P Orsolini,&nbsp;G Filice","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Leptospirosis, anthropo-zoonosis ubiquitously widespread, is a social and economic problem still to be solved. The experimental and therapeutic employment of many antibiotics has largely been tested \"in vitro\" and \"in vivo\". In the following research we tried to evaluate, by experimental \"in vitro\" method, the sensitivity difference of three serovar strains of Leptospira interrogans to two macrolides, Erythromycin and Josamycin, compared with Penicillin. From standard cultures, previously treated with serial dilution of these antibiotics, the MIC and MSC, as quantitative parameters, have been stated. For the qualitative evaluation of the damages induced at ultrastructural level by the drug activity. Electron Microscopy investigations were performed on specimens from cultures treated for 6 hr with twice and tenfold the MSC. The present research confirms the good sterilizing efficaciousness \"in vitro\" of the tested macrolides (MSC less than 1 mcg/ml) and their different activity pathway.</p>","PeriodicalId":75613,"journal":{"name":"Bollettino dell'Istituto sieroterapico milanese","volume":"68 1","pages":"17-23"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13635714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Modulation of macrophage functions by corynebacterium parvum preparations. 小棒状杆菌制剂对巨噬细胞功能的调节。
Bollettino dell'Istituto sieroterapico milanese Pub Date : 1989-01-01
L Bonina, D Delfino, G B Costa, A Arena
{"title":"Modulation of macrophage functions by corynebacterium parvum preparations.","authors":"L Bonina,&nbsp;D Delfino,&nbsp;G B Costa,&nbsp;A Arena","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Corynebacterium parvum has been used as an anti-tumor agent in both animal experiments and clinical trials; nevertheless it is not well established the mechanism of action, since both T-dependent and T-independent ways of action have been proposed. On the other hand, mononuclear phagocytic system activation cannot be ruled out in the immunomodulation carried out by C. parvum. In the attempt to better understand the effects of C. parvum on macrophage functions, experiments were carried out on tumor-bearing rats with a decline of peritoneal macrophage activities as a result of the tumor development. For this purpose three different preparations of C. parvum were investigated: a) the first preparation was obtained by heat-inactivation of bacteria at 60 degrees C for 1h; b) the second by formalin-treatment of bacteria; c) the third obtained from Burrough Wellcome as a formaline-killed suspension in 0.01% w/v thiomersal saline. Each preparation was used at 7 mg/ml dry weight and administered subcutaneously, three days after tumor grafting. The results showed that all three preparations were capable to hinder the tumor mass growth. Macrophage functions as phagocytosis, intracellular killing, intrinsic and extrinsic anti-Herpes Simplex Virus activities, were restored to normal values by each of the preparations used. Nevertheless, concerning the chemotactic activity a discrepancy was noted among the three preparations, since the heat-inactivated one was able to carried out a complete restoration.</p>","PeriodicalId":75613,"journal":{"name":"Bollettino dell'Istituto sieroterapico milanese","volume":"68 1","pages":"62-6"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13635719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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