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Archives de l'Institut Pasteur d'Algerie. Institut Pasteur d'Algerie最新文献

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[Detection of human papillomavirus (H.P.V.) DNA in genital lesions using molecular hybridization]. [人乳头瘤病毒(hpv)的检测]利用分子杂交技术研究生殖器病变中的DNA。
Archives de l'Institut Pasteur d'Algerie. Institut Pasteur d'Algerie Pub Date : 1992-01-01
S Meguenni, S el-Mehdaoui, D Bandoui, A Bouguermouh, A Allouache, A Bendib, A Chouiter, T Djenaoui, N Lalliam, A Bouhadef
{"title":"[Detection of human papillomavirus (H.P.V.) DNA in genital lesions using molecular hybridization].","authors":"S Meguenni,&nbsp;S el-Mehdaoui,&nbsp;D Bandoui,&nbsp;A Bouguermouh,&nbsp;A Allouache,&nbsp;A Bendib,&nbsp;A Chouiter,&nbsp;T Djenaoui,&nbsp;N Lalliam,&nbsp;A Bouhadef","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Detection of human papilloma virus in genitals lesions by molecular hybridization. Some H.P.V. types are sexually transmitted and infect genital organs. We have used molecular hybridization to examine the distribution of H.P.V. 6 or II and H.P.V. 16 in benign, premalignant and malignant genital lesions from 344 patients. The frequency of H.P.V. 16 positive cases increases as the cervical lesions progress to malignancy: 57/78 are positive (73%) in the carcinomas, 29/83 are positive (35%) in mild or moderate dysplasia. The majority of benign condylomata acuminata harbors DNA of other types, namely H.P.V. 6 and II.</p>","PeriodicalId":75535,"journal":{"name":"Archives de l'Institut Pasteur d'Algerie. Institut Pasteur d'Algerie","volume":"58 ","pages":"291-7"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12512773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Production of a vaccine against enterotoxemia from Clostridium perfringens strains isolated in the field]. [利用田间分离的产气荚膜梭菌菌株生产抗肠毒血症疫苗]。
Archives de l'Institut Pasteur d'Algerie. Institut Pasteur d'Algerie Pub Date : 1992-01-01
S Cherfaoui, B Kadra
{"title":"[Production of a vaccine against enterotoxemia from Clostridium perfringens strains isolated in the field].","authors":"S Cherfaoui,&nbsp;B Kadra","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We have isolated eight strains of C. perfringens from cases of enterotoxaemia. Five of these strains have revealed themselves toxic with respective types (type \"A\":2, type \"C\":2, type \"D\":1). In order to produce anti-enterotoxaemia vaccine, we have proceeded at the cultivation in fermenter of isolated strains and reference strains CWA 35, CWC and CWD AF. At the end of fermentation, we have evaluated the two following parameters: obtained biomass, and toxin titers. With the two classes of strains we reached an important biomass but toxins titers relatively weak comparatively to that which is usually required. It will be necessary then, to demonstrate the immunogen value of the produced vaccines by testing their efficacity.</p>","PeriodicalId":75535,"journal":{"name":"Archives de l'Institut Pasteur d'Algerie. Institut Pasteur d'Algerie","volume":"58 ","pages":"205-24"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12483136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Various isolation and enrichment media for isolating Yersinia enterocolitica]. 【分离小肠结肠炎耶尔森菌的各种分离和富集培养基】。
Archives de l'Institut Pasteur d'Algerie. Institut Pasteur d'Algerie Pub Date : 1992-01-01
Z Guechi, Z Nehari
{"title":"[Various isolation and enrichment media for isolating Yersinia enterocolitica].","authors":"Z Guechi,&nbsp;Z Nehari","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Study of different isolation and enrichment media for recovering Yersinia enterocolitica has been carried out. The best data have been obtained after enrichment in phosphate buffer with sorbitol and bile salts, followed by a decontamination with Aulisio's KOH and streaking into Hectoen medium.</p>","PeriodicalId":75535,"journal":{"name":"Archives de l'Institut Pasteur d'Algerie. Institut Pasteur d'Algerie","volume":"58 ","pages":"27-39"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12483141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Informatics: applications in bacteriology]. [信息学:细菌学中的应用]。
Archives de l'Institut Pasteur d'Algerie. Institut Pasteur d'Algerie Pub Date : 1989-01-01
M Neggazi, D Kezerli, A Chachou, D Hettal, L Demou, F Bouikni, K Rahal
{"title":"[Informatics: applications in bacteriology].","authors":"M Neggazi,&nbsp;D Kezerli,&nbsp;A Chachou,&nbsp;D Hettal,&nbsp;L Demou,&nbsp;F Bouikni,&nbsp;K Rahal","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The subject of this work is to describe one of the possible applications of the informatics system in medical bacteriology, its exploitation in edition of the analysis results and in the management of produced stocks. The informatics is presently a very productive tool giving a precise information, a very important time gain and a better control of the costs. This work admit two chapters. In the first one, we give general notions of informatics and in the second one we describe the applications.</p>","PeriodicalId":75535,"journal":{"name":"Archives de l'Institut Pasteur d'Algerie. Institut Pasteur d'Algerie","volume":"57 ","pages":"201-11"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13636332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Anaerobic bacteria in chronic renal insufficiency: apropos of a case]. 厌氧菌在慢性肾功能不全中的应用[附一例]。
Archives de l'Institut Pasteur d'Algerie. Institut Pasteur d'Algerie Pub Date : 1989-01-01
S Merad
{"title":"[Anaerobic bacteria in chronic renal insufficiency: apropos of a case].","authors":"S Merad","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We relate a case of chronic renal insufficiency complicated by urinary anaerobic infection caused by Bacteroides vulgatus and Peptococcus asaccharolyticus.</p>","PeriodicalId":75535,"journal":{"name":"Archives de l'Institut Pasteur d'Algerie. Institut Pasteur d'Algerie","volume":"57 ","pages":"249-53"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13636334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Activity of cefotaxime and ceftriaxone and of imipenem, pefloxacin and norfloxacin on 13 species of bacteria]. [头孢噻肟、头孢曲松及亚胺培南、培氟沙星、诺氟沙星对13种细菌的活性]。
Archives de l'Institut Pasteur d'Algerie. Institut Pasteur d'Algerie Pub Date : 1989-01-01
K Rahal
{"title":"[Activity of cefotaxime and ceftriaxone and of imipenem, pefloxacin and norfloxacin on 13 species of bacteria].","authors":"K Rahal","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A first comparative in vitro study of Cefotaxime and Ceftriaxone activities on thirteen species of Algerian bacteria is done. These two third generation Cephalosporins have the same activity. A second comparative study of two Quinolones (Pefloxacin, Norfloxacin ) and a Penicillin (Imipenem) is done. Imipenem give satisfying results and is very stable against beta lactamases. In vitro Norfloxacin has a better activity than Pefloxacin.</p>","PeriodicalId":75535,"journal":{"name":"Archives de l'Institut Pasteur d'Algerie. Institut Pasteur d'Algerie","volume":"57 ","pages":"213-48"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13636333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Detection of oligoclonal immunoglobulins in the cerebrospinal fluid using agar gel immunoelectrophoresis with silver salt staining]. 【银盐染色琼脂凝胶免疫电泳检测脑脊液中寡克隆免疫球蛋白】。
Archives de l'Institut Pasteur d'Algerie. Institut Pasteur d'Algerie Pub Date : 1989-01-01
H Merahba, C Allal, M Ghaffor, H Rabhi, M C Abbadi
{"title":"[Detection of oligoclonal immunoglobulins in the cerebrospinal fluid using agar gel immunoelectrophoresis with silver salt staining].","authors":"H Merahba,&nbsp;C Allal,&nbsp;M Ghaffor,&nbsp;H Rabhi,&nbsp;M C Abbadi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Detection of oligoclonal immunoglobulins in multiple sclerosis cerebrospinal fluid (MS-CSF) seems to be an important test in the biological diagnosis of the disease. In our laboratory, the classical agarose gel electrophoretic technic allowed the detection of CSF oligoclonal bands in only 40% of the MS patients. This relatively low percentage in comparison with those obtained by other investigators led us to develop a much more resolving electrophoretic technic: an agarose gel isoelectric focusing (IEF) with silver staining. By this method, we detected oligoclonal immunoglobulins in 43 (43%) of 100 MS-CSF exhibiting only polyclonal patterns on classical electrophoresis. Oligoclonal banding appeared particularly in patients with inflammatory type profile (36/60) according to Schuller's classification, but also in those with normal (5/26) or inflammatory transudative (2/8) type profiles. MS-CSF with oligoclonal immunoglobulins on IEF had a higher IgG/Albumin ratio (p = 0.01) than those with polyclonal immunoglobulins, while the mean IgG levels were not significantly different (p = 0.07). These results support the diagnostic usefulness of the IgG/Albumin ratio. Agarose IEF appears to be a useful technic in the detection of oligoclonal immunoglobulins and may be applied at least to CSF of patients with clinical signs of multiple sclerosis.</p>","PeriodicalId":75535,"journal":{"name":"Archives de l'Institut Pasteur d'Algerie. Institut Pasteur d'Algerie","volume":"57 ","pages":"163-72"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13632394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Diagnosis of rabies infection in animals using monoclonal antibodies]. [应用单克隆抗体诊断动物狂犬病]。
Archives de l'Institut Pasteur d'Algerie. Institut Pasteur d'Algerie Pub Date : 1989-01-01
O Akacem, A Taril, A Benelmouffok, A Bemansour, P Couillin, M Brahimi, M Benhassine
{"title":"[Diagnosis of rabies infection in animals using monoclonal antibodies].","authors":"O Akacem,&nbsp;A Taril,&nbsp;A Benelmouffok,&nbsp;A Bemansour,&nbsp;P Couillin,&nbsp;M Brahimi,&nbsp;M Benhassine","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Two monoclonal antibodies (M.A.), specific for viral nucleocapsid, the M.A. D-20 and the M.A. D-43 raised against a fixed strain of rabies virus (C.V.S. 11), have been tested in parallel with a standard antirabies serum (S.A.R.) in diagnosis of animal rabies virus infection. 44 brain imprints from animals which died from rabies were tested by indirect immunofluorescent technique with monoclonal antibodies. Constant correlation has been found between the M.A. D-43 and the S.A.R. in the diagnosis of animal rabies virus infection in all cases studied. For M.A. D-20, concordance of results with S.A.R. was found only in limited number of cases.</p>","PeriodicalId":75535,"journal":{"name":"Archives de l'Institut Pasteur d'Algerie. Institut Pasteur d'Algerie","volume":"57 ","pages":"9-23"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13636339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Evaluation of molecular hybridization tests and DNA restriction enzyme analysis for the detection and typing of herpes simplex virus (HSV)]. [分子杂交试验和DNA限制性内切酶分析在单纯疱疹病毒(HSV)检测和分型中的评价]。
Archives de l'Institut Pasteur d'Algerie. Institut Pasteur d'Algerie Pub Date : 1989-01-01
S Meguenni, D Bandoui, Y Lahlou, J el Kolli, A Bouguermouh
{"title":"[Evaluation of molecular hybridization tests and DNA restriction enzyme analysis for the detection and typing of herpes simplex virus (HSV)].","authors":"S Meguenni,&nbsp;D Bandoui,&nbsp;Y Lahlou,&nbsp;J el Kolli,&nbsp;A Bouguermouh","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Restriction enzyme analysis of DNA and blot hybridization tests were applied for the identification of 90 herpes simplex viruses isolated. The results obtained by those methods correlated completely with typing by monoclonal antibodies in an indirect immunofluorescent assay.</p>","PeriodicalId":75535,"journal":{"name":"Archives de l'Institut Pasteur d'Algerie. Institut Pasteur d'Algerie","volume":"57 ","pages":"49-60"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13706236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cells expressing intracytoplasmic immunoglobulins in secreting and hyposecreting cases of alpha chain disease. 表达胞浆内免疫球蛋白的细胞在α链病的分泌和分泌不足病例中的作用。
Archives de l'Institut Pasteur d'Algerie. Institut Pasteur d'Algerie Pub Date : 1989-01-01
H Rabhi, M Ghaffor, M Benhalima-Bouali, M C Abbadi
{"title":"Cells expressing intracytoplasmic immunoglobulins in secreting and hyposecreting cases of alpha chain disease.","authors":"H Rabhi,&nbsp;M Ghaffor,&nbsp;M Benhalima-Bouali,&nbsp;M C Abbadi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Alpha chain disease proteins (ACDP) originated probably from secreting plasma-cells predominantly present in diffuse and massive enteromesenteric lymphoid infiltration. Very decreased levels of abnormal alpha chain molecules were detected in sera of patients with immunoblastic lymphoma occurring in the late course of the disease. A direct correlation might exist between the proportion of cells bearing intracytoplasmic IgA determinants and the serum amounts of alpha chain disease protein. Relevant evidence raised from study of proliferating lymphoid cells using the unlabeled peroxidase anti-peroxidase method of immunocytochemistry. The percentage of cells expressing intracytoplasmic alpha chains was found to be greater readily secreting case than in hyposecreting case of alpha chain disease. Furthermore, the cells from secreting situation exhibited much more pronounced specific staining, indicative of probably more active synthesis state. Taken together with histological data, these results suggested a possible late evolutionary pathway without detectable intracytoplasmic and serum alpha chain disease protein. They might also support the hypothesis that alpha chain disease and mediterranean lymphoma were different evolutionary phases of the same entity.</p>","PeriodicalId":75535,"journal":{"name":"Archives de l'Institut Pasteur d'Algerie. Institut Pasteur d'Algerie","volume":"57 ","pages":"125-34"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13661730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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