Acta Universitatis Palackianae Olomucensis Facultatis Medicae最新文献_第9页

Analysis of chemokine gene expression in lung cells by polymerase chain reaction. 聚合酶链反应分析趋化因子基因在肺细胞中的表达。

Acta Universitatis Palackianae Olomucensis Facultatis Medicae Pub Date : 1999-01-01

M Petrek

引用次数: 0

Selected prognostic factors of long-term renal graft function. 移植肾功能长期预后因素的选择。

Acta Universitatis Palackianae Olomucensis Facultatis Medicae Pub Date : 1999-01-01

K Krejcí

引用次数: 0

[Hematologic findings and value of value of endogenous creatinine in patients following kidney transplantation]. [肾移植术后患者的血液学表现及内源性肌酐的价值]。

Acta Universitatis Palackianae Olomucensis Facultatis Medicae Pub Date : 1999-01-01

J Zadrazil, P Bachleda, K Krejcí

{"title":"[Hematologic findings and value of value of endogenous creatinine in patients following kidney transplantation].","authors":"J Zadrazil, P Bachleda, K Krejcí","doi":"","DOIUrl":"","url":null,"abstract":"We have studied serum erythropoietin (EPO) levels, serum creatinine and blood hemoglobin and hematocrit levels in the group of 140 patients after kidney transplantation. All patients received conventional immunosuppressive therapy consisting of cyclosporine (Sandimmune Neoral), azathioprin (Imuran) and steroids (Prednison). The aim of the study was to investigate the relationship between serum EPO, serum creatinine and hematologic parameters. Complete restoration of hemoglobin and hematocrit levels was obtained in 99 patients (70.7%), moderate degree of anaemia with average hemoglobin level 114.7 +/- 12.3 g/l was seen in 37 patients (26.4%) and four patients (2.9%) had posttransplant erythrocytosis. Normal hemoglobin values are generally restored during 3 months after successful renal transplantation. Statistical analysis confirmed significant correlation (p < 0.001) between graft function and hematologic parameters, but none correlation was found neither between serum EPO levels and serum creatinine and not between serum EPO levels and hemoglobin/hematocrit values. Plasma creatinine is not reliable indicator of renal EPO secretory capacity. The degree of correction of anemia is limited by the stage of graft function and correlates with the serum creatinine concentration. The results suggest, that the restoration of normal excretory graft function after transplantation improves bone marrow response to EPO and leads to normalization of erythropoietic activity.","PeriodicalId":75423,"journal":{"name":"Acta Universitatis Palackianae Olomucensis Facultatis Medicae","volume":"142 ","pages":"103-6"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21594073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of BCL-2 in the developing kidney of human embryos and fetuses qualitative and quantitative study. BCL-2在人胚胎和胎儿肾脏发育中的表达的定性和定量研究。

Acta Universitatis Palackianae Olomucensis Facultatis Medicae Pub Date : 1999-01-01

V Lichnovský, B Erdösová, K Punkt, M Zapletal

{"title":"Expression of BCL-2 in the developing kidney of human embryos and fetuses qualitative and quantitative study.","authors":"V Lichnovský, B Erdösová, K Punkt, M Zapletal","doi":"","DOIUrl":"","url":null,"abstract":"Twelve human embryos and fetuses aged of 7-30 weeks of intrauterinal life were examined to determine the expression of bcl-2 gene in the developing kidney. Tissue samples were routinely processed and three-step indirect immunohistochemical method was used for the detection of Bcl-2 protein. End-point cytophotometry was performed with computer-controlled microscope photometer with a scanning table and the mean relative absorbance of the final product of peroxidase reaction was determined and taken as a measure of Bcl-2 expression. The morphometric evaluation was carried out from the TV display using Weibel s universal hexagonal raster and we determined the relative volume of Bcl-2 positive structures in the various zones of the embryonal kidney. The aim of our research was mapping of the Bcl-2 occurrence in the developing kidney of human embryos and fetuses. The Bcl-2 protein is involved in the regulation of apoptosis and its effect is antiapoptotic. The highest Bcl-2 expression was proved in the cells of metanephrogenic blastema. The lower occurrence of Bcl-2 positive cells was demonstrated in proximal tubules analges+ and it was almost on the borderline of detection in branches of ureteral bud. In the fetal period the marked Bcl-2 expression was maintained in the epithelial cells of proximal tubules analges.","PeriodicalId":75423,"journal":{"name":"Acta Universitatis Palackianae Olomucensis Facultatis Medicae","volume":"142 ","pages":"61-4"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21594147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of successful combined renal and pancreatic transplantation on diabetic retinopathy. 肾胰联合移植成功对糖尿病视网膜病变的影响。

Acta Universitatis Palackianae Olomucensis Facultatis Medicae Pub Date : 1998-01-01

T Sosna, F Saudek, Z Domínek

{"title":"Effect of successful combined renal and pancreatic transplantation on diabetic retinopathy.","authors":"T Sosna, F Saudek, Z Domínek","doi":"","DOIUrl":"","url":null,"abstract":"Unlabelled: The treatment of diabetic retinopathy (DR) is one of the most challenging problems in ophthalmology. The possibility of modulating DR by successful combined kidney and pancreas transplantation thus holds an attractive promise for the ophthalmologist. From June 1983 until April 1997, a total of 86 combined kidney and pancreas transplantation procedures were performed at the Prague-based Institute for Clinical and Experimental Medicine. All recipients are on close follow-up in terms of their ophthalmic status. However, evaluation of the effect of transplantation is problematic because of the advanced status of DR prior to the procedure. We divided the transplant recipients into two groups according to type of transplantation. In Group I (segmental transplantation), proliferative DR was present in 100% eyes, 70% had undergone vitrectomy, and there were 21% of blind eyes. All eyes had been treated by laser. In this group, long-term stabilization of the finding was observed in three patients. In Group II (whole pancreas transplantation), proliferative and non-proliferative DR was diagnosed in 86% and 14%, respectively. There were 12% of blind eyes, and 70% had been treated by laser prior to transplantation. After a successful transplantation, stabilization was found in 60%, improvement in 18%, and deterioration in 22% of eyes in this group. The stabilization and improvement can be explained by subsequent normoglycemia (HbAlc 5.6%). By contrast, we were unable to provide a satisfactory explanation for the deterioration and progression of these findings. While the effect of immunosuppression on DR remains unclear, it obviously accelerates the existing cataract.Conclusion: Successful combined transplantation has a beneficial effect on DR and is worthwhile even for patients at the end stage on account of its beneficial psychosocial effect and prevention of dolorous glaucoma. However, many effects of the procedure on the eye of diabetics remain to be identified in future studies.","PeriodicalId":75423,"journal":{"name":"Acta Universitatis Palackianae Olomucensis Facultatis Medicae","volume":"141 ","pages":"75-7"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20601713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Signalling towards cell wall synthesis in budding yeast. 出芽酵母细胞壁合成信号。

Acta Universitatis Palackianae Olomucensis Facultatis Medicae Pub Date : 1998-01-01

V Raclavský

{"title":"Signalling towards cell wall synthesis in budding yeast.","authors":"V Raclavský","doi":"","DOIUrl":"","url":null,"abstract":"The budding yeast Saccharomyces cerevisiae has long proved to be a very useful model in cell biology. Its cell morphology is established and maintained at least in part by the cell wall, a rigid but dynamic structure that affords mechanical protection. Although fungal cell walls represent an unique phenomenon, recent progress in research has shown striking parallels between yeast and mammalian cells in the area of cell morphogenesis and proliferation. Further studies promise to shed common light on the processes of cell morphogenesis including the intersections with proliferation control. This review focuses on the recent progress in this promising area in the yeast Saccharomyces cerevisiae. The process of cell wall synthesis in Saccharomyces cerevisiae was reviewed by several authors recently. Briefly, the cell wall represents a complex structure of cross-linked chitin, beta-(1,6)-d-glucan, beta-(1,3)-D-glucan and mannoproteins. Chitin and beta-(1,3)-D-glucan are synthesized by enzymatic complexes at the cell membrane and extruded into the periplasmic space, mannoproteins are synthesized along the yeast secretory pathway, and the site of beta-(1,6)-D-glucan synthesis is still unknown. The principal motif which interconnects individual cell wall constituents was recently identified by Kollár et al. The mechanisms of cross-linking of the polymers in the wall remain unknown, however. Recently, nevertheless, substantial progress has been achieved in understanding the signalling pathways which target the cell wall construction.","PeriodicalId":75423,"journal":{"name":"Acta Universitatis Palackianae Olomucensis Facultatis Medicae","volume":"141 ","pages":"7-16"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20601267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutants of Trichophyton mentagrophytes with changed optimum cultivation temperature. 最适培养温度变化的毛癣菌突变体。

Acta Universitatis Palackianae Olomucensis Facultatis Medicae Pub Date : 1998-01-01

V Chalupová

引用次数: 0

Utility of short-term heart rate variability for prediction of sudden cardiac death after acute myocardial infarction. 短期心率变异性在预测急性心肌梗死后心源性猝死中的应用。

Acta Universitatis Palackianae Olomucensis Facultatis Medicae Pub Date : 1998-01-01

J Kautzner, P St'ovícek, Z Anger, J Savlíková, M Malik

{"title":"Utility of short-term heart rate variability for prediction of sudden cardiac death after acute myocardial infarction.","authors":"J Kautzner, P St'ovícek, Z Anger, J Savlíková, M Malik","doi":"","DOIUrl":"","url":null,"abstract":"Heart rate variability (HRV) computed from 24-hour ECG recording has been associated with an increased risk of malignant arrhythmias after MI. To make HRV analysis more practical, we evaluated prospectively prognostic role of short-term HRV in comparison with other risk stratifiers. Study population consisted of 48 patients with acute MI (mean age 59.6 +/- 10.6 years, 38 males), who were off betablockers. All patients underwent 30-minute ECG recording at supine rest on day 2 and 5 after admission, between 9 and 11 a.m. One ECG channel from a commercial bedside monitor was A/D converted, and subsequently analysed using a purpose-built interactive software. Short-term HRV was computed as the standard deviation of all normal-to-normal RR intervals (SDNN) as well as the square root of the mean of the sum of the squares of differences between adjacent normal RR intervals (rMSSD). Left ventricular ejection fraction (LVEF, in %) was determined using 2D-echocardiography. During one-year follow up, 5 patients (10.4%) died of sudden cardiac death (SCD) and one of non-cardiac death. Subjects who died of SCD presented with significantly lower SDNN parameter on day 5 (28.8 +/- 4.3 vs 39 +/- 18.4, p < 0.006) and similar trend was revealed for rMSSD (12.22.8 vs 24.321, N.S.). Similarly, LVEF was significantly decreased in these patients (35.4 +/- 5.5 vs 49.7 +/- 11.3, p < 0.007). Positive predictive accuracy for prediction of SCD was 17% for rMSSD, 20% for SDNN, 29% for LVEF, and 40% for combination of depressed SDNN (< or = 33 ms) and LVEF (< or = 40). In conclusion, depressed HRV computed from short-term predischarge ECG recordings obtained under standardised conditions is associated with an increased risk of SCD. Such predictive power is substantially increased in combination with depressed LVEF, and this approach seems to be effective as a simple screening method to identify high risk subjects.","PeriodicalId":75423,"journal":{"name":"Acta Universitatis Palackianae Olomucensis Facultatis Medicae","volume":"141 ","pages":"69-73"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20601712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphology of non-ventilated transplanted rat lungs. 非通气移植大鼠肺形态。

Acta Universitatis Palackianae Olomucensis Facultatis Medicae Pub Date : 1998-01-01

J Dusek, F Kolár, B Korecký

引用次数: 0

A contribution to the types of branching and anastomoses of the splenic artery in human spleen. 对人脾脾动脉分支和吻合类型的贡献。

Acta Universitatis Palackianae Olomucensis Facultatis Medicae Pub Date : 1998-01-01

A Holibková, L Machálek, D Houserková, V Růzicka

引用次数: 0