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Acta pathologica et microbiologica Scandinavica. Section B, Microbiology最新文献

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Purine metabolism in Neisseria meningitidis. 3. Utilization of exogenous hypoxanthine, guanine and xanthine. 脑膜炎奈瑟菌嘌呤代谢。3.外源次黄嘌呤、鸟嘌呤和黄嘌呤的利用。
Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 1975-10-01
S Jyssum
{"title":"Purine metabolism in Neisseria meningitidis. 3. Utilization of exogenous hypoxanthine, guanine and xanthine.","authors":"S Jyssum","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Whole cells of Neisseria meningitidis have been found to utilize exogenous radioactive hypoxanthine, guanine and xanthine. When hypoxanthine was the precursor, the pools of both the adenine and the guanine 5'-ribonucleotides were labelled. Guanine and xanthine were utilized with labelling of the pool of the guanine 5'-ribonucleotides only. Crude extracts from N. meningitidis were found to have activities corresponding to hypoxanthine phosphoribosyltransferase (EC 2.4.2.8) and another phosphoribosyltransferase which seems to exhibit specificity for guanine and xanthine. Crude extracts phosphorylated guanosine 5'-monophosphate to guanosine 5'-triphosphate in the presence of adenosine 5'-triphosphate (ATP) and MgCl2.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"83 5","pages":"397-406"},"PeriodicalIF":0.0,"publicationDate":"1975-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11997472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The serology of Pseudomonas aeruginosa analysed by means of quantitative immunoelectrophoretic methods. III. Reproducibility of a polyvalent P. aeruginosa reference standard-antigen. 采用定量免疫电泳方法对铜绿假单胞菌进行血清学分析。3多价铜绿假单胞菌参比标准抗原的可重复性。
Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 1975-10-01
N Hoiby
{"title":"The serology of Pseudomonas aeruginosa analysed by means of quantitative immunoelectrophoretic methods. III. Reproducibility of a polyvalent P. aeruginosa reference standard-antigen.","authors":"N Hoiby","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The reproducibility of a polyvalent Pseudomonas aeruginosa antigen (St-Ag) composed of a mixture of antigens from 4 O groups of this bacterium has been studied. Ten batches of St-Ag were produced, and each of these and each of the 10 batches of antigens from the 4 strains of P. aeruginosa were compared with St-Ag batch 1 by means of quantitative immunoelectrophoretic methods and a polyvalent antiserum (St-Ab) raised against St-Ag. Fifty-three of the 55 antigens of St-Ag were stable and could be reproduced with reasonable precision in all 10 batches, and 3 of the 4 strains of P. aeruginosa were stable in antigen composition in all batches. One of the strains (0-5A) had lost 2 antigens in the last 5 batches, and the concentrations of 7 other antigens were simultaneously changed, reflecting a smooth-rough dissociation. The disappearance of the 2 antigens in the latest 5 batches of 0-5A was also reflected in similar changes in the antigen composition of the latest 5 batches of St-Ag.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"83 5","pages":"433-42"},"PeriodicalIF":0.0,"publicationDate":"1975-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11997475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The serology of Pseudomonas aeruginosa analysed by means of quantitative immunoelectrophoretic methods. II. Comparison of the antibody response in man against thirteen O groups of Ps. aeruginosa. 采用定量免疫电泳方法对铜绿假单胞菌进行血清学分析。2人对铜绿假单胞菌13组O型抗体反应的比较。
Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 1975-08-01
N Hoiby
{"title":"The serology of Pseudomonas aeruginosa analysed by means of quantitative immunoelectrophoretic methods. II. Comparison of the antibody response in man against thirteen O groups of Ps. aeruginosa.","authors":"N Hoiby","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The occurrence of antibodies against antigens prepared from strains representing 13 O groups of Pseudomonas aeruginosa and against a polyvalent Ps. aeruginosa antigen (St-Ag) has been investigated in sera from 100 patients. By means of fused rocket immunoelectrophoresis with intermediate gel it was found that the humoral immune response against Ps. aeruginosa resulting in precipitating antibodies will be detected by St-Ag as well as by any other of the antigen samples investigated. Six of the sera contained group-specific antibodies which were revealed by only one of the antigen samples used and not by St-Ag. These six sera were further studied by means of various quantitative immunoelectrophoretic methods using St-Ag as well as antigens prepared from the infecting Ps. aeruginosa strain in the patient concerned. In all six sera, only one extra precipitin could be detected using antigens prepared from the homologous strain instead of St-Ag. This extra precipitin corresponded presumably to group-specific O-antigens not included in St-Ag. In sera from patients, these group-specific antibodies were always accompanied by antibodies against antigens common to all strains of Ps. aeruginosa.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"83 4","pages":"328-34"},"PeriodicalIF":0.0,"publicationDate":"1975-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11995793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of neutralizing and immunoprecipitating activity in guinea pig antisera against Herpes simplex virus types 1 and 2. 豚鼠抗单纯疱疹病毒1型和2型血清中和和免疫沉淀活性的比较。
Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 1975-08-01 DOI: 10.1111/j.1699-0463.1975.tb00111.x
S Jeansson, B F Vestergaard
{"title":"Comparison of neutralizing and immunoprecipitating activity in guinea pig antisera against Herpes simplex virus types 1 and 2.","authors":"S Jeansson,&nbsp;B F Vestergaard","doi":"10.1111/j.1699-0463.1975.tb00111.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1975.tb00111.x","url":null,"abstract":"<p><p>Guinea pig antisera against Herpes simplex virus (HSV) types 1 and 2 were compared in neutralization tests and crossed immunoelectrophoresis. It was found that the HSV type specific neutralization of guinea pig antisera could be associated with the precipitating activity to HSV type 1 and 2 glycoproteins.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"83 4","pages":"343-6"},"PeriodicalIF":0.0,"publicationDate":"1975-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1699-0463.1975.tb00111.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11387037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Application of the passive haemolysis test for the determination of rubella virus antibodies. 被动溶血试验测定风疹病毒抗体的应用。
Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 1975-08-01 DOI: 10.1111/j.1699-0463.1975.tb00114.x
K Skaug, I Orstavik, J C Ulstrup
{"title":"Application of the passive haemolysis test for the determination of rubella virus antibodies.","authors":"K Skaug,&nbsp;I Orstavik,&nbsp;J C Ulstrup","doi":"10.1111/j.1699-0463.1975.tb00114.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1975.tb00114.x","url":null,"abstract":"<p><p>A passive haemolysis test for the determination of antibodies against rubella virus haemagglutinin is presented. According to this method, the principle of radial immunodiffusion techinque is applied. Rubella haemagglutinin-coated chick erythrocytes in the agarose gel were lysed by the diffusing positive sera of complement at 37 degrees C. The passive haemolysis test was compared with the conventional haemagglutination inhibition method, and the diameter of the haemolysis zones was shown to be a direct measure of the quantity of antibody added to the well. A plot of the log (HI titre) against the zone diameter gives a straight line. There is no need to remove nonspecific haemagglutination inhibitors. However, all serum samples must be inactivated at 56 degrees C for 30 minutes before testing. Tests of 200 serum samples from healthy women showed a good correlation between the haemagglutination inhibition titre and the antibody titre determined by the passive haemolysis technique. Twenty-one samples with a haemagglutination inhibition titre less than 10 were also negative in the passive haemolysis test. With the exception of one, all sera with a positive haemagglutination inhibition titre showed a positive haemolysis reaction. The method was found to be as specific and as sensitive as the haemagglutination inhibition test. In addition, the technique is rapid and simple for quantitative studies of antibodies against rubella virus.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"83 4","pages":"367-72"},"PeriodicalIF":0.0,"publicationDate":"1975-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1699-0463.1975.tb00114.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12278476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 39
Radiorespirometric studies in genus Neisserai. I. The catabolism of glucose. 奈瑟菌属的放射肺量测定研究。葡萄糖的分解代谢。
Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 1975-08-01
E Holten
{"title":"Radiorespirometric studies in genus Neisserai. I. The catabolism of glucose.","authors":"E Holten","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The catabolism of glucose in selected Neisseria species was studied by radiorespirometry. The Entner-Doudoroff and pentose phosphate pathways were operating in all species, the greater part of the substrate being routed through the former, somewhat dependent on the medium used. Acetate was oxidized via the tricarboxylic acid cycle. In all species, a fraction of the triose was recycled through fructose phosphates to glucose 6-phosphate. Phosphate inhibited the oxidation of acetate. In media devoid of phosphate and sodium (N. meningitidis and N. gonorrhoeae) or of phosphate and potassium (other saccharolytic Neisseria), the conversion of triose to pyruvate was inhibited. In these media the catabolism of glucose proceeded slowly, and no substrate was used for biosynthetic purposes. The results point to a difference in the regulation of glucose metabolism in pathogenic and non-pathogenic Neisseria.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"83 4","pages":"353-66"},"PeriodicalIF":0.0,"publicationDate":"1975-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12333446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The serology of Pseudomonas aeruginosa analysed by means of quantitative immunoelectrophoretic methods. I. Comparison of thirteen O groups of Ps. aeruginosa, with a polyvalent Ps. aeruginosa antigen-antibody reference system. 采用定量免疫电泳方法对铜绿假单胞菌进行血清学分析。1 .铜绿假单胞菌13个O组的比较，采用多价铜绿假单胞菌抗原抗体参比系统。
Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 1975-08-01
N Hoiby
{"title":"The serology of Pseudomonas aeruginosa analysed by means of quantitative immunoelectrophoretic methods. I. Comparison of thirteen O groups of Ps. aeruginosa, with a polyvalent Ps. aeruginosa antigen-antibody reference system.","authors":"N Hoiby","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Serologic cross-reactions between 26 strains of Pseudomonas aeruginosa representing 13 O groups were studied by various quantitative immunoelectrophoretic techniques. As reference system was used a polyvalent Ps. aeruginosa antigen and a corresponding rabbit antiserum. Fifty-one (93 per cent) of the 55 Ps. aeruginosa antigens in the reference system were present in all the strains and corresponding antibodies in the reference system could be completely absorbed by all the strains. Complete cross-reactivity was also found between antigens of the reference system and 3 of the 4 antigens present only in some of the strains. The last of the 4 antigens not present in all the strains could only absorb part of the corresponding antibodies in the reference system. Absorption experiments with whole heat-killed bacteria indicate that this antigen is related to the O group antigens of Ps. aeruginosa. None of the antigens of the reference system were related to the mucoid substance produced by some strains of this bacterium.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"83 4","pages":"321-7"},"PeriodicalIF":0.0,"publicationDate":"1975-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11995792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Yersinia enterocolitica in small rodents from Norway, Sweden and Finland. 挪威、瑞典和芬兰小型啮齿动物的小肠结肠炎耶尔森菌。
Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 1975-08-01 DOI: 10.1111/j.1699-0463.1975.tb00110.x
G Kapperud
{"title":"Yersinia enterocolitica in small rodents from Norway, Sweden and Finland.","authors":"G Kapperud","doi":"10.1111/j.1699-0463.1975.tb00110.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1975.tb00110.x","url":null,"abstract":"<p><p>The purpose of the present investigation was to study the occurrence of Yersinia enterocolitica (Y.e.) among small rodents and shrews (Soricidae) from Norway, Sweden and Finland. During a period of one year beginning in the autumn of 1973, animals from seven localities were examined. Twenty-four strains of Y.e. were isolated from 551 small rodents. Isolations were made from six of the seven localities and from six of the nine small rodent species examined. Pooled faeces samples from 397 animals yielded 12 strains. Faeces from 154 small rodents were separately investigated: Twelve (8 per cent) of these animals harboured Y.e. No isolations were made from 52 shrews. Most of the strains showed antigenic relationship to O-serotypes previously reported from untreated drinking water in Norway. Two strains were antigenically related to O-serotype 3 but differed biochemically from the strains found in man and swine. The results indicate that Y.e. has a widespread distribution among small rodents in Norway, Sweden and Finland.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"83 4","pages":"335-42"},"PeriodicalIF":0.0,"publicationDate":"1975-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1699-0463.1975.tb00110.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12333445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 47
Common enterobacterial antigen in Yersinia enterocolitica. 小肠结肠炎耶尔森菌常见肠杆菌抗原。
Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 1975-08-01 DOI: 10.1111/j.1699-0463.1975.tb00116.x
J A Maeland, A Digranes
{"title":"Common enterobacterial antigen in Yersinia enterocolitica.","authors":"J A Maeland,&nbsp;A Digranes","doi":"10.1111/j.1699-0463.1975.tb00116.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1975.tb00116.x","url":null,"abstract":"<p><p>Production of the common enterobacterial antigen (CA) by strains of Yersinia enterocolitica (Y.e.) serotypes 3 and 9 (Winblad), by strains of 5 different Y.e. serogroups (Wauters) and various other bacteria was examined. Antibody against CA was raised by immunization of rabbits with E. coli O 14. Extract prepared from S. typhimurium was used for the sensitization of sheep erythrocytes with CA. Absorption and haemagglutination inhibition experiments showed that CA could be detected in heat extracts from all Y.e. strains examined, and in that from Yersinia pseudotuberculosis. CA was not detected in extracts from Pasteurella multocida, Francisella tularensis, Brucella abortus, Acinetobacter calcoaceticus or Pseudomonas aeruginosa. Anti-CA antibodies could not be demonstrated in serum from rabbits immunized with Y.e. bacteria, but were demonstrated in serum from rabbits immunized with a CA-containing fraction of the Y.e. extract. The possibility of participation of anti-CA antibody in indirect haemagglutination tests for detection of antibody to Y.e. O antigens is emphasized.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"83 4","pages":"382-6"},"PeriodicalIF":0.0,"publicationDate":"1975-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1699-0463.1975.tb00116.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12278477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 21
The use of protein A-containing staphylococci sensitized with anti-meningococcal antibodies for grouping Neisseria meningitidis and demonstration of meningococcal antigen in cerebrospinal fluid. 用抗脑膜炎球菌抗体致敏的含蛋白a葡萄球菌对脑膜炎奈瑟菌进行分组和脑脊液中脑膜炎球菌抗原的检测。
Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 1975-08-01 DOI: 10.1111/j.1699-0463.1975.tb00117.x
P Olcén, D Danielsson, J Kjellander
{"title":"The use of protein A-containing staphylococci sensitized with anti-meningococcal antibodies for grouping Neisseria meningitidis and demonstration of meningococcal antigen in cerebrospinal fluid.","authors":"P Olcén,&nbsp;D Danielsson,&nbsp;J Kjellander","doi":"10.1111/j.1699-0463.1975.tb00117.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1975.tb00117.x","url":null,"abstract":"<p><p>The co-agglutination technique, utilizing antibody coated protein A-containing staphylococci, was successfully adapted to grouping N. meningitidis strains. It was found to give more clear-cut results than the standard slide test, especially in the case of strains isolated from throat specimens. The co-agglutination technique has also other advantages over the standard slide test in the grouping of meningococci: minor influence by auto-agglutination, higher specificity, easy performance and low consumption of specific antisera. Preliminary results also showed that the co-agglutination technique could be applied for the rapid detection of meningococcal antigen in cerebrospinal fluid.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"83 4","pages":"387-96"},"PeriodicalIF":0.0,"publicationDate":"1975-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1699-0463.1975.tb00117.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11995746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 69
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