Acta pathologica et microbiologica Scandinavica. Section B, Microbiology最新文献

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Bovine protothecosis. A brief report of ten cases. 牛protothecosis。十个案例的简短报告。
J Bodenhoff, P S Madsen
{"title":"Bovine protothecosis. A brief report of ten cases.","authors":"J Bodenhoff,&nbsp;P S Madsen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Prototheca zopfii was isolated repeatedly from milk samples from ten cows (of a herd of 192 dairy cows) with reduced milk yield and indurated mammary glands. The strain was moderately sensitive to streptomycin, polymyxin and gentamycin, but resistant or relatively resistant to other antibiotics and antimycotics commonly used in clinical practice. An attempt to treat the infection with Ethidium bromide, which was found effective in vitro, did not succeed. The number of Prototheca excreted decreased, but a complete cure was not obtained. In histological sections of the udder, Prototheca cells were demonstrated both intracellularly and interstitially.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"86 1","pages":"51-2"},"PeriodicalIF":0.0,"publicationDate":"1978-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11867194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Light and electron microscopy on the sporulation of the oocysts of Eimeria brunetti. II. Development into the sporocyst and formation of the sporozoite. 布鲁氏艾美耳球虫卵囊产孢的光镜和电镜观察。2孢子囊的发育和孢子子的形成。
D J Ferguson, A Birch-Andersen, W M Hutchinson, J C Siim
{"title":"Light and electron microscopy on the sporulation of the oocysts of Eimeria brunetti. II. Development into the sporocyst and formation of the sporozoite.","authors":"D J Ferguson,&nbsp;A Birch-Andersen,&nbsp;W M Hutchinson,&nbsp;J C Siim","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The later stages of sporulation in oocysts of Eimeria brunetti were examined in samples which had been allowed to sporulate at 27 degrees C for 24, 36 and 48 hours. It was observed that the sporoblasts became ellipsoidal and the nucleus underwent the final division. A nucleus with associated Golgi bodies was not observed at either end of the organism. The cytoplasm was limited by two unit membranes and contained rough endoplasmic reticulum, dense bodies, electron translucent vacuoles and mitochondria. The first evidence of sporozoite formation was the appearance of a dense plaque at either end of the organism. This appeared in the vicinity of the nuclei, and adjacent to the limiting membrane of the soroblast. At this stage the sporocyst wall was still unformed. Then the two sporozoites were formed from opposite ends of the organism by growth of the dense plaques and invaginations of the plasmalemma which thus formed the pellicles of the developing sporozoites. A conoid and subpellicular microtubules were observed at this stage as development continued, a number of vacuoles were found between the nucleus and the conoid. These vacuoles constituted the precursors of the rhoptries and micronemes. At the same stage a large dense body had appeared within the forming sporozoite. As the sporozoite developed, this body, anterior refractile body, is followed by the nucleus and another dense body which formed the posterior refractile body. During this period, the thin sporocyst wall was formed and Stieda and sub-Stieda bodies were now present at one end of the sporocyst. Each mature sporocyst contained two sporozoites.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"86 1","pages":"13-24"},"PeriodicalIF":0.0,"publicationDate":"1978-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11867188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Light and electron microscopy on the sporulation of the oocysts of Eimeria brunetti. I. Development of the zygote and formation of the sporoblasts. 布鲁氏艾美耳球虫卵囊产孢的光镜和电镜观察。合子的发育和孢子母细胞的形成。
D J Ferguson, A Birch-Andersen, W M Hutchinson, J C Siim
{"title":"Light and electron microscopy on the sporulation of the oocysts of Eimeria brunetti. I. Development of the zygote and formation of the sporoblasts.","authors":"D J Ferguson,&nbsp;A Birch-Andersen,&nbsp;W M Hutchinson,&nbsp;J C Siim","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The initial stages of sporulation in oocysts of Eimeria brunetti were examined in samples sporulated at 27 degrees C for 0, 12 and 24 hours. The initial zygote was found to be roughly spherical and to contain a number of polysaccharide granules which were congregated in one region of the organism. The cytoplasm also contained some strands of rough endoplasmic reticulum together with a number of mitochondria, some Golgi bodies, and some electron translucent vacuoles. The nucleus was large, with amorphous nucleoplasm and a nucleous. The cytoplasmic mass of the zygote was limited by a single unit membrane which possessed some micropores. After initiation of the sporulation, the metabolic activity of the organism appeared to increase as evidenced by the augmentation in the cytoplasm of the amounts of rough endoplasmic reticulum, number of Golgi bodies, and the appearance of polyribosomes. However, at this stage, the presence of large spherical bodies (anlagen of the refractile bodies of the sporozoites) constituted the most obvious change in the cytoplasm of the organism. After nuclear division the daughter nuclei were situated well separated in the cytoplasm and the polysaccharide granules were evenly distributed throughout the cytoplasm of the zygote. Eventually four sporoblasts were formed by invaginations of the limiting membrane. Each sporoblast was limited by a unit membrane and contained a nucleus, and the same cytoplasmic organelles as found in the zygote. The development of the sporoblast was initially accompanied by the appearance of a second limiting membrane.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"86 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"1978-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11867186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Human interferon and cell growth inhibition. II. Biological and physico-chemical properties of the growth inhibitory component. 人干扰素与细胞生长抑制。2生长抑制成分的生物和物理化学性质。
H Dahl
{"title":"Human interferon and cell growth inhibition. II. Biological and physico-chemical properties of the growth inhibitory component.","authors":"H Dahl","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Human leukocyte interferon (IF) inhibits the growth rate of homologous cells in culture. The growth inhibitory component, formerly separated from the antiviral component of human leukocyte IF by adsorption chromatography on albumin-agarose, is investigated. The properties of the component are compared with the accepted characteristics of IF. The growth inhibition is caused by a small molecule, unstable to pH 2 treatment with HCl, but stable to trichloro-acetic acid (TCA) and to proteolytic enzymes. The siolated growth inhibitor seems to be a dimer of molecular weight (mol wt.) approximately 2300. It is activated by heat treatment and lacks species specificity. The discrepancies in the properties of the growth inhibitor before and after separation from IF are discussed.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"85B 1","pages":"54-60"},"PeriodicalIF":0.0,"publicationDate":"1977-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11238070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Oxidase positive rods from cases of suspected gonorrhoea. A comparison of conventional, gas chromatographic and genetic methods of identification. 疑似淋病病例的氧化酶阳性棒。常规、气相色谱和遗传鉴别方法的比较。
Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 1977-02-01 DOI: 10.1111/J.1699-0463.1977.TB01671.X
Kjell Bøvre, Norman Hagen, BJØRN PETER Berdal, E. Jantzen
{"title":"Oxidase positive rods from cases of suspected gonorrhoea. A comparison of conventional, gas chromatographic and genetic methods of identification.","authors":"Kjell Bøvre, Norman Hagen, BJØRN PETER Berdal, E. Jantzen","doi":"10.1111/J.1699-0463.1977.TB01671.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1977.TB01671.X","url":null,"abstract":"Genito-urethral specimens from 3260 women and 1170 men, with ailments suggestive of gonorrhoea, were examined for growth of oxidase positive rodshaped bacteria, as well as of gonococci. Moraxella osloensis was identified in 26 cases (0.64 per cent of women and 0.43 per cent of men). Three patients harboured phenylalanine negative (or weakly reacting) and tryptophan deaminase negative M. phenylpyrouvica and, in three cases, a Flavobacterium species was detected. Among six oropharyngeal specimens from patients suspected of gonorrhoea, two yielded growth of oxidase positive rods, Kingella kingae and Neisseria elongata, respectively, N. gonorrhoeae was isolated from 537 patients, i.e., 12.1 per cent of all cases. The isolates of oxidase positive rods were in most cases completely identified by streptomycin resistance transformation. On this basis, the diagnostic reliability of some morphological and cultural-biochemical tests and gas chromatography was examined. Gas chromatographic analysis of fatty acid and alcohol composition of whole cells proved distinctive of species defined genetically, irrespective of confusing behaviour of some strains in other tests.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"29 1","pages":"27-37"},"PeriodicalIF":0.0,"publicationDate":"1977-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89588915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
Experimental toxoplasmosis in mice and rabbits. Virulence and cyst formation of Toxoplasma gondii. 小鼠和家兔的实验性弓形虫病。刚地弓形虫的毒力和囊肿形成。
E K Pettersen
{"title":"Experimental toxoplasmosis in mice and rabbits. Virulence and cyst formation of Toxoplasma gondii.","authors":"E K Pettersen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Avirulent Toxoplasma gondii were made virulent by passages in mice, and then attenuated by storage. In contrast to the avirulent parasites, attenuated organisms did not appear to form cysts in mice and rabbits, although parasites could be isolated from such animals as long as three months after their inoculation. Rabbits infected with parasites of attenuated virulence had high antibody levels, and survived challenge with virulent Toxoplasma gondii. Cysts were not detected in the brains of such rabbits following this second infection. By following the generation of Toxoplasma gondii in mouse peritoneal cavities, it was found that attenuated parasites resembled avirulent more than virulent organisms. Although the effect of attenuation was mainly on the parental generation of parasites, some genetic effect seemed to be involved as well.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"85B 1","pages":"95-102"},"PeriodicalIF":0.0,"publicationDate":"1977-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12028638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Oxidase positive rods from cases of suspected gonorrhoea. A comparison of conventional, gas chromatographic and genetic methods of identification. 疑似淋病病例的氧化酶阳性棒。常规、气相色谱和遗传鉴别方法的比较。
K Bovre, N Hagen, B P Berdal, E Jantzen
{"title":"Oxidase positive rods from cases of suspected gonorrhoea. A comparison of conventional, gas chromatographic and genetic methods of identification.","authors":"K Bovre,&nbsp;N Hagen,&nbsp;B P Berdal,&nbsp;E Jantzen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Genito-urethral specimens from 3260 women and 1170 men, with ailments suggestive of gonorrhoea, were examined for growth of oxidase positive rodshaped bacteria, as well as of gonococci. Moraxella osloensis was identified in 26 cases (0.64 per cent of women and 0.43 per cent of men). Three patients harboured phenylalanine negative (or weakly reacting) and tryptophan deaminase negative M. phenylpyrouvica and, in three cases, a Flavobacterium species was detected. Among six oropharyngeal specimens from patients suspected of gonorrhoea, two yielded growth of oxidase positive rods, Kingella kingae and Neisseria elongata, respectively, N. gonorrhoeae was isolated from 537 patients, i.e., 12.1 per cent of all cases. The isolates of oxidase positive rods were in most cases completely identified by streptomycin resistance transformation. On this basis, the diagnostic reliability of some morphological and cultural-biochemical tests and gas chromatography was examined. Gas chromatographic analysis of fatty acid and alcohol composition of whole cells proved distinctive of species defined genetically, irrespective of confusing behaviour of some strains in other tests.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"85B 1","pages":"27-37"},"PeriodicalIF":0.0,"publicationDate":"1977-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11609217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sorption of Streptococcus faecium to glass. 粪链球菌在玻璃上的吸附。
D Orstavik
{"title":"Sorption of Streptococcus faecium to glass.","authors":"D Orstavik","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A method has been developed by which to study the sorption of Streptococcus faecium to soda-lime cover glasses. Conditions were chosen to minimize the influence on sorption of bacterial polymer production, passive sorption being studied rather than attachment mediated by metabolic activities. Sorption of S. faecium increased with increasing temperature (to 50 degrees C), time, and cell concentration, but equilibrium apparently was not reached even after incubation for 8 hours or at a cell concentration of 3 X 10(10) per ml. Sorption increased with solute molarity up to 0.1 M concentration of NaCl and KCl, indicating an effect of the electrical double layers on the apposition of cells to the glass surface. Desorption of bacteria could be obtained after multiple washings of the glasses in buffer or by the action of Tween 80, but not if sorbed bacteria were left in distilled water, various salt solutions, urea, or in suspensions of unlabelled bacteria. It was concluded that sorption occurred as a result of chemical interactions between the glass and the cell surface. Tween 80 at a concentration of 1 per cent inhibited sorption to 26 per cent of buffer controls, 2 M urea was less effective, and 1 M NaCl was without effect. It is suggested that hydrophobic interactions may be of importance in the binding of S. faecium to glass.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"85B 1","pages":"38-46"},"PeriodicalIF":0.0,"publicationDate":"1977-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11609218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sorption of Streptococci to glass: Effects of macromolecular solutes. 玻璃对链球菌的吸附:大分子溶质的影响。
D Orstavik
{"title":"Sorption of Streptococci to glass: Effects of macromolecular solutes.","authors":"D Orstavik","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Macromolecular solutes (albumin, concanavalin A, whole saliva, serum) caused impaired sorption of Streptococcus faecium and Streptococcus sanguis to glass. The inhibitory effect resided chiefly with interactions of the solutes with the glass surface. In the case of sorption of S. sanguis to glass in the presence of parotid fluid, the inhibitory effect was counteracted by a specific attachment of S. sanguis cells mediated by some component(s) of the parotid fluid. Agglutination of the test organisms was in general accompanied by inhibition of sorption. However, when small or unstable aggregates were formed, the number of cells adhering on the glass surface was increased. The findings are discussed with reference to the colonization of teeth by oral bacteria.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"85B 1","pages":"47-53"},"PeriodicalIF":0.0,"publicationDate":"1977-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11609219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of paramyxovirus-specific haemolysis-inhibiting antibodies separate from haemagglutinating-inhibiting and neuraminidase-inhibiting antibodies. 2. NDV and mumps virus haemolysis-inhibiting antibodies. 副粘病毒特异性溶血抑制抗体与血凝抑制抗体和神经氨酸酶抑制抗体的分离鉴定。2. 新城疫和流行性腮腺炎病毒溶血抑制抗体。
C Orvell
{"title":"Identification of paramyxovirus-specific haemolysis-inhibiting antibodies separate from haemagglutinating-inhibiting and neuraminidase-inhibiting antibodies. 2. NDV and mumps virus haemolysis-inhibiting antibodies.","authors":"C Orvell","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Egg-grown Newcastle disease (NDV) and mumps virus were used for preparation of rabbit hyperimmune sera against purified whole virus and projectionless virus particles. These sera and convalescent sera after natural NDV and mumps infections in chickens and human subjects, respectively, were studied in haemolysis-inhibition (HLI), haemagglutination-inhibition (HI) and neuraminidase-inhibition (NI) tests both before and after absorption with Tween 80-ether (TE) treated virus preparations. In addition, neutralization tests using the different sera were carried out. HI and NI antibodies and the major population of neutralizing antibodies in convalescent sera were removed by absorption with TE treated virus material without changing the titre of non-HI HLI antibodies. Rabbit hyperimmune sera directed against projectionless virus particles exhibited HLI antibody titres in marked excess of HI and NI antibody titres, whereas this was not found in sera against whole virus. Absorption with TE treated virus material resulted in removal of all demonstrable antibody activities in sera against whole virus. The corresponding absorption of sera against projectionless particles eliminated HI antibodies without changing the titre of non-Hi HLI antibodies. In rabbit hyperimmune sera, HI antibodies were of primary importance in neutralization tests. After addition of anti-gamma globulin to the test, an efficient neutralization was observed if mumps non-HI HLI antibodies were used whereas this was not found if NDV non-HI HLI antibodies were used.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"84B 6","pages":"451-7"},"PeriodicalIF":0.0,"publicationDate":"1976-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12179184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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