{"title":"[Surface ultrastructural cyclic change of the human uterine glandular and lining epithelia. A scanning electron microscopic study (author's transl)].","authors":"K Adachi, E Akashi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The purpose of this study is to take advantage of scanning electron microscopy on the topographic alterations of the human uterine glandular epithelium which occur during the various stages of the menstrual cycle. 1) Both glandular and lining epithelia were mainly covered with nonciliated cells which reflected the cyclical changes during the menstrual cycle. In the early proliferative phase, microvilli were short and the apical cellular membrane slightly protruded, however microvilli and the apical cellular membrane gradually developed toward the end of proliferative phase. Compared with the surface epithelium, the glandular epithelium showed a monotonous change because of the uniform surface structure. 2) Numerous ciliogenesis can be observed on the lining epithelium in the late proliferative phase. In contrast, ciliogenesis could not be recognized in the glandular epithelium. 3) In the glandular epithelium, an apocrine type of secretion was recognized for several days after ovulation and they were seen to cluster occasionally. It was suggested that the secretion activity differed among various parts of the endometrial gland. 4) The menopausal phase was divided into two groups: the early stage of the postmenopausal phase resembled the early proliferative phase and had a fusiform and flattened cells with slender microvilli. Ciliated cells were seen in clusters. On the other hand, the advanced postmenopausal phase had uniform flattened cells with sparse microvilli and ciliated cells were extremely rare. 5) The lining epithelium of a female fetus was recognized with a slight protrusion of the apical cellular membrane with long microvilli.</p>","PeriodicalId":75398,"journal":{"name":"Acta obstetrica et gynaecologica Japonica","volume":"33 3","pages":"321-30"},"PeriodicalIF":0.0,"publicationDate":"1981-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18226947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S Akahori, K Eguchi, T Hasegawa, T T Lin, K Sekiba
{"title":"Estimation of gestosis of pregnancy (EPH-gestosis), relationship between fetal development and gestosis index.","authors":"S Akahori, K Eguchi, T Hasegawa, T T Lin, K Sekiba","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Gestosis index score at delivery was compared with factors reflecting the fetal development in a series of 95 patients with EPH-gestosis collected in Okayama University Medical School in 1975-1979. The prediction of small for date (SFD) was performed by means of multivariate analysis of 10 variables, i.e. gestosis index, maternal body weight, height, uterine fundal length, abdominal circumference, maternal age at delivery and urinary estriol within one week prior to delivery. The result was that gestosis index was as useful as uterine fundal length in predicting SFD. The more gestosis index score increased, the more markedly the fetal development ws disturbed. Especially in the cases with scoring above 4 and in those with hypertension and proteinuria, the incidence of SFD increased obviously. No correlation between gestosis index and neonatal asphyxia was noticed. From the growth pattern of uterine fundal length and BPD, intrauterine growth retardation (IUGR) in pregnancy with EPH-gestosis occurred mainly within the third trimester of pregnancy. The functional development of the fetus with EPH-gestosis was evaluated with the use of urinary estriol level and fetal heart rate (FHR) monitoring. In the cases with EPH-gestosis scoring above 4, extreme disturbance of functional development of the fetus was observed.</p>","PeriodicalId":75398,"journal":{"name":"Acta obstetrica et gynaecologica Japonica","volume":"33 3","pages":"395-402"},"PeriodicalIF":0.0,"publicationDate":"1981-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18209206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Studies on specific markers of hormone-dependence in endometrial carcinoma : estradiol receptors and uterine peroxidase (author's transl)].","authors":"S Morikawa, S Sekiya, H Takamizawa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Both cytosol estradiol-17 beta (E2) receptors and uterine peroxidase were detected in normal uterine tissues of rat and hamster, however, they were not detected when the tissues were cultivated in vitro. Uterine peroxidase was specifically induced by the administration of E2 in these normal uterine tissues in vivo. On the other hand, malignant tissues formed by the transplantation of uterine and mammary adenocarcinoma cells showed hormone-independence. Neither cytosol E2 receptors nor peroxidase was detected in these malignant tissues. These results indicate that, in addition of E2 receptors, peroxidase induction by E2 is a specific marker for hormone-dependence in both normal and abnormal uterine tissues.</p>","PeriodicalId":75398,"journal":{"name":"Acta obstetrica et gynaecologica Japonica","volume":"33 3","pages":"339-43"},"PeriodicalIF":0.0,"publicationDate":"1981-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18226948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of second look operation for malignant ovarian tumors.","authors":"M Yakushiji, M Abe, A Tsunawaki, J Umezu, T Kato","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75398,"journal":{"name":"Acta obstetrica et gynaecologica Japonica","volume":"33 3","pages":"411-6"},"PeriodicalIF":0.0,"publicationDate":"1981-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17322122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Relationship between the onset of labor and prostaglandins in rabbit tissues before and after delivery (author's transl)].","authors":"N Makimura, K Kato, I Nagata, K Seki","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>1) The studies were performed to elucidate the mechanism of onset of labor. It was investigated in relation to the movement of prostaglandins in individual tissues of pregnant rabbits before and after labor with the onset of labor. 2) The rabbit uterus duplex used is convenient material to investigate the movement of PGs in the same rabbit at the different term. 3) The concentrations of PGs were measured by RIA. 4) The concentrations of PGs in blood and amniotic fluid were not essential to explain account for the mechanism of onset labor. 5) From the results of the PGs values before and after labor, we have been considered that the relative PGs at the onset of labor were PG E1 and PG E2 in the placenta. We infer that PG F2 alpha plays a leading role in uterine contraction, because the large amount of PG F2 alpha presented in decidual parietalis during delivery, and PG E1 and PG E2 play a role in uterine contraction, too.</p>","PeriodicalId":75398,"journal":{"name":"Acta obstetrica et gynaecologica Japonica","volume":"33 3","pages":"377-83"},"PeriodicalIF":0.0,"publicationDate":"1981-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18226014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Initiation of labor and the fetal oxytocin contribution (author's transl)].","authors":"S Kawai","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This study was designed to evaluate the fetal oxytocin (OT) contribution to the instigation and maintenance of human labor. Plasma samples together with placental tissues and myometria were collected in an effort to determine immunoreactive OT concentrations in plasma, oxytocinase activities in placenta and 3H-labeled OT uptake in myometria respectively and the following results were obtained. OT concentrations in the umbilical arterial plasma (UA) at 20 to 24 weeks were doubled at 37 to 42 weeks suggesting the increase of this hormone to occur in the latter half of pregnancy. The UA values at term were significantly higher than that of maternal peripheral and umbilical venous plasma (MPV and UV). OT concentrations in UA and UV showed a linear correlation but not with MPV. The study of labor (+) and labor (-) subjects were performed in cesarean sections as well as in vaginal deliveries. The OT concentrations in UA was higher in cesarean sections with labor pains than without labor pains. In vaginal deliveries, the OT concentrations were much higher. The uptake of biologically active OT estimated by 3H-labeled OT uptake of 20,000 xg precipitate fraction of nonpregnant, first trimester and term myometria were 10.5 plus or minus 3.1, 18.3 plus or minus 6.6 and 35.4 plus or minus 6.6% respectively. While no significant changes of placental oxytocinase activities were found between these specimens. The activities seemed to be lower in cases without labor pain than those with labor pains. These findings suggest that there is OT release from the fetus during labor to participate in the instigation and maintenance of labor with increase of the uterine sensitivity to OT, however it appears to be more active in maintaining labor than in initiating it.</p>","PeriodicalId":75398,"journal":{"name":"Acta obstetrica et gynaecologica Japonica","volume":"33 3","pages":"385-94"},"PeriodicalIF":0.0,"publicationDate":"1981-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18226015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ultrastructure of the nidatory site in tubal pregnancy.","authors":"K Kawagoe, T Kawana, S Sakamoto","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Nidatory site of pre-abortive tubal pregnancy, obtained from 41 years old women was studied in both scanning and transmission electron microścopes. The nidatory site was basically composed of anchoring villi, trophoblastic column and shell, fibrinoid layer and marked round cell infiltration. In spite of the histologically normal appearance of the chorionic villi, villous syncytiotrophoblast ultrastructurally showed slight tendency of degeneration, being concurrent with blighted ovum. The trophoblast at the trophoblastic column and shell contained a great amount of glycogen which decreased at the distal part near and in the fibrinoid layer. The degenerating trophoblast were surrounded by massive fibrin bundles and fibrin bundles and fibrin-like deposits. The more the degeneration of the trophoblast proceeded, the more the fibrin deposits accumulated around the trophoblast. The trophoblast which ran over the fibrinoid layer was scarcely observed and if there, it showed marked degenerative appearance. These findings suggest the possible role of fibrinoid layer: protection of maternal tissue from trophoblastic invasion, not the protection of fetus from maternal immunological attack. No direct contact such as tight junction, desmosome and so on between trophoblast and maternal cell was observed in the present study, suggesting the formation of special connection with the allogeneic cell, one of the unique characters of the trophoblast, is expressed only when it is primitive in the early stage of development.</p>","PeriodicalId":75398,"journal":{"name":"Acta obstetrica et gynaecologica Japonica","volume":"33 3","pages":"403-10"},"PeriodicalIF":0.0,"publicationDate":"1981-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18226016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Effect of anticancer drugs on in vitro survival of cell lines derived from various gynecologic tumors (author's transl)].","authors":"S Sekiya, H Takamizawa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Effect of various anticancer drugs on in vitro survival of gynecologic tumor cells were investigated by using established cell lines derived from human ovarian adenocarcinoma (HOC-21), endometrial adenocarcinoma (HEC-1B), and cervical squamous cell carcinoma (SKG-1). As compared with other established cell lines, 1) HOC-21 cells were more sensitive to Cis-platinum, Adriamycin, and actinomycin-D. 2) HEC-1B cells were more sensitive to progesterone. 3) SKG-1 cells were more sensitive to bleomycin and mitomycin-C. These results are consistent with the clinical effects of various anticancer drugs, and established cell lines derived from various human gynecologic tumors are useful to select effective anticancer drugs in vitro.</p>","PeriodicalId":75398,"journal":{"name":"Acta obstetrica et gynaecologica Japonica","volume":"33 3","pages":"373-6"},"PeriodicalIF":0.0,"publicationDate":"1981-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17228118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Study of oxytocin receptor activities in human myometrial preparation (author's transl)].","authors":"H Sakamoto, H Fukai, Y Oyama, K Den, S Takagi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This study was designed to investigate the oxytocin (OT) receptor activities in the human myometrial specimen throughout the course of pregnancy and in labor. The 3H-labeled OT with adequate specific activity (24 Ci/mM) and biologic activity (350 IU/mg) was employed for the OT radioreceptor assay and the apparent dissociation constant (Kds) and the number of binding sites (NBS) were analyzed by Scatchard plot. These activities were predominantly found in 20,000 xg pellets of the myometrial specimens and its binding activities were 2.2 +/- 0.1 nmol/mg protein that were assessed to be composed of two binding fractions: High-affinity--low capacity receptor fraction and low affinity--high capacity fraction. The gestational increase of the binding affinity was significant and so was the increase of the capacity, and the Kd was 1.87 +/- 0.30 nM and the NBS was 1.50 +/- 0.50 pmol/mg protein in the full term specimens and both were significantly higher than those of the nonpregnant specimens (p less than 0.01). When active labor was initiated, a significant decrease of the binding capacity (0.74 +/- 0.21 pmol/mg protein) was found, regardless of the mode of onset of labor; i.e. spontaneous or induced labor, while the binding affinity remained unchanged. These findings suggest that the gestational increase of the uterine sensitivity to OT is due to the increase of both binding affinity and capacity of the OT receptors. And there are OT and OT receptor interaction actively involved during the active stage of labor and this interaction seems to be coupled with the contractile process but is apparently modified when active labor had been already initiated.</p>","PeriodicalId":75398,"journal":{"name":"Acta obstetrica et gynaecologica Japonica","volume":"33 3","pages":"344-52"},"PeriodicalIF":0.0,"publicationDate":"1981-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17322121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}