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Acta microbiologica Polonica最新文献

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Primary resistance of Helicobacter pylori to antimicrobial agents in Polish children. 波兰儿童幽门螺杆菌对抗菌药物的原发性耐药性。
Acta microbiologica Polonica Pub Date : 2002-01-01
Elzbieta Rozynek, Katarzyna Dzierzanowska-Fangrat, Danuta Celińska-Cedro, Paulina Jóźwiak, Kazimierz Madaliński, Danuta Dzierzanowska
{"title":"Primary resistance of Helicobacter pylori to antimicrobial agents in Polish children.","authors":"Elzbieta Rozynek,&nbsp;Katarzyna Dzierzanowska-Fangrat,&nbsp;Danuta Celińska-Cedro,&nbsp;Paulina Jóźwiak,&nbsp;Kazimierz Madaliński,&nbsp;Danuta Dzierzanowska","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Helicobacter pylori resistance to antimicrobial agents is an important factor compromising the efficacy of treatment. Therefore the aims of our study were: to determine the prevalence of H. pylori resistance to clarithromycin, metronidazole, amoxycillin and tetracycline in children prior to eradication therapy, to compare different methods of susceptibility testing and to detect mutations responsible for clarithromycin resistance. During 1996-2000, 259 H. pylori strains were isolated from antral gastric biopsies. Susceptibility to antimicrobials was determined by the agar dilution method and the Etest. Mutations in the 23S rRNA gene associated with clarithromycin resistance were analysed by PCR-RFLP and direct sequencing. Overall, ninety-six strains (37%) were resistant to metronidazole, 50 strains (19.3%) were resistant to clarithromycin, and 20 strains (7.7%) were simultaneously resistant to both drugs. All cultured isolates were sensitive to amoxycillin and only one isolate (0.4%) was resistant to tetracycline. The agar dilution method and the Etest showed a perfect category correlation for clarithromycin and 4% discrepancies for metronidazole. Primary resistance to clarithromycin was mainly associated with an A2143G mutation in the 23S rRNA gene of H. pylori. The study highlights the high prevalence of H. pylori primary resistance to clarithromycin in Polish children, which implies a need for pretreatment susceptibility testing.</p>","PeriodicalId":75388,"journal":{"name":"Acta microbiologica Polonica","volume":"51 3","pages":"255-63"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22247499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antimicrobial activity of substituted 2-trifluoromethyl- and 2-pentafluoroethylbenzimidazoles. 取代的2-三氟甲基和2-五氟乙基苯并咪唑的抗菌活性。
Acta microbiologica Polonica Pub Date : 2002-01-01
Renata Wolinowska, Jolanta Zajdel-Dabrowska, Bohdan J Starościak, Zygmunt Kazimierczu
{"title":"Antimicrobial activity of substituted 2-trifluoromethyl- and 2-pentafluoroethylbenzimidazoles.","authors":"Renata Wolinowska,&nbsp;Jolanta Zajdel-Dabrowska,&nbsp;Bohdan J Starościak,&nbsp;Zygmunt Kazimierczu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Antibacterial and antifungal activity of 2-trifluoromethyl- and 2-pentafluoroethylbenzimidazoles, including a number of newly obtained derivatives, were examined by diffusion method (inhibition area diameter in solid agar medium) and minimum inhibitory concentration (MIC, in liquid and agar medium). Some of the derivatives tested affected fungal colony morphology and exerted genotoxic effects in bacteria. Of the tested compounds, 5,6-dichlorosubstituted derivatives appeared the most active against the majority of microorganisms used.</p>","PeriodicalId":75388,"journal":{"name":"Acta microbiologica Polonica","volume":"51 3","pages":"265-73"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22247500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fatty acid composition from an epiphytic strain of Fusarium oxysporum associated with algal crusts. 与藻壳相关的尖孢镰刀菌附生菌株的脂肪酸组成。
Acta microbiologica Polonica Pub Date : 2002-01-01
Richard J Ellis, Jan M C Geuns, Robert Zarnowski
{"title":"Fatty acid composition from an epiphytic strain of Fusarium oxysporum associated with algal crusts.","authors":"Richard J Ellis,&nbsp;Jan M C Geuns,&nbsp;Robert Zarnowski","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The fatty acid composition from Fusarium oxysporum B1, a fungal epiphyte from algal crusts, was determined. The most prominent fatty acids found in the mycelium were 18:1 omega9c, 16:0 and 18:0. These compounds amounted 47% of total fatty acids, whereas remaining fatty acids identified, including beta-hydroxylated, iso-branched, diunsaturated and odd-numbered derivatives were present in minor amounts.</p>","PeriodicalId":75388,"journal":{"name":"Acta microbiologica Polonica","volume":"51 4","pages":"391-4"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22352438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Green fluorescent protein as a molecular marker in microbiology. 绿色荧光蛋白在微生物学中的分子标记作用。
Acta microbiologica Polonica Pub Date : 2002-01-01
Stanisław Józef Rosochacki, Marzena Matejczyk
{"title":"Green fluorescent protein as a molecular marker in microbiology.","authors":"Stanisław Józef Rosochacki,&nbsp;Marzena Matejczyk","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Molecular markers such as: lacZ (b-galactosidase), xylE (catechol 2,3-dioxygenase), lux (bacterial luciferase), luc (insect luciferase), phoA (alkaline phosphatase), gusA and gurA (beta-glucuronidase), gfp (green fluorescent protein), bla (beta-lactamase) and other antibiotic resistance markers, heavy metals resistance genes are commonly used in environmental microorganisms research (Errampaii et al., 1998; Kohler et al., 1999). Most of these markers require one or more substrates, complex media and/or expensive equipment for detection. The gfp gene is widely used as a marker because of its very useful properties such as high stability, minimal toxicity, non-invasive detection and the ability to generate the green light without addition of external cofactors and without application of expensive equipment. Various applications of that reporter gene were showed starting from monitoring of microorganism's survival in complex biological systems such as activated sludge to biodegradation of chemical compounds in soil. GFP allowed the detection, determination of spatial location and enumeration of bacterial cells from diverse environmental samples such as biofilm and water. The gfp as a biomarker was very useful in monitoring of gene expression and protein localisation in bacterial cells, too. The techniques with using gfp marker promise to supply a better understanding of environmental processes. It can make possible to use that knowledge in designing more effective and more efficient methods of biodegradation of toxic compounds from different environments.</p>","PeriodicalId":75388,"journal":{"name":"Acta microbiologica Polonica","volume":"51 3","pages":"205-16"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22247915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Intraperitoneal fluid accumulation induced by Clostridium perfringens alpha-toxin (phospholipase C). 产气荚膜梭菌α毒素(磷脂酶C)引起的腹腔积液。
Acta microbiologica Polonica Pub Date : 2002-01-01
Tsuneo Maegawa, Xingmin Wang, Tadahiro Karasawa, Masahiko Zuka, Hiroyuki Kita, Shinichi Nakamura
{"title":"Intraperitoneal fluid accumulation induced by Clostridium perfringens alpha-toxin (phospholipase C).","authors":"Tsuneo Maegawa,&nbsp;Xingmin Wang,&nbsp;Tadahiro Karasawa,&nbsp;Masahiko Zuka,&nbsp;Hiroyuki Kita,&nbsp;Shinichi Nakamura","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We report that the intraperitoneal injection of Clostridium perfringens alpha-toxin into mice induces ascites. This phenomenon was monitored by measuring fluid volume and analyzing hematologic data. The mouse toxicity test provides a simple and useful model for examining C. perfringens alpha-toxin-induced vascular permeability.</p>","PeriodicalId":75388,"journal":{"name":"Acta microbiologica Polonica","volume":"51 4","pages":"387-9"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22352437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Numerical modeling of ferrous-ion oxidation rate in Acidithiobacillus ferrooxidans ATCC 23270: optimization of culture conditions through statistically designed experiments. 酸性氧化亚铁硫杆菌ATCC 23270中铁离子氧化速率的数值模拟:通过统计设计实验优化培养条件。
Acta microbiologica Polonica Pub Date : 2002-01-01
Yasser R Abdel-Fattah, Wael R Abdel-Fattah, Rogelio Zamilpa, James R Pierce
{"title":"Numerical modeling of ferrous-ion oxidation rate in Acidithiobacillus ferrooxidans ATCC 23270: optimization of culture conditions through statistically designed experiments.","authors":"Yasser R Abdel-Fattah,&nbsp;Wael R Abdel-Fattah,&nbsp;Rogelio Zamilpa,&nbsp;James R Pierce","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Statistically designed experimental strategy has been performed in order to evaluate and optimize nutritional and environmental parameters that affect ferrous ion oxidation rate in Acidithiobacillus ferrooxidans ATCC 23270. Plackett-Burman design was carried out to evaluate efficiently the biological significance of 10 culture conditions influencing ferrous-ion oxidation rate of A. ferrooxidans grown for 5 days in shake-flask batch mode on the newly modified 9-K media. Among ten fermentation factors examined, the most significant variables influencing ferrous-ion oxidation rate were statistically elucidated to be pH and calcium nitrate as positive contributors, whereas trace metals solution and potassium chloride were the most significant negative contributors. The optimal levels of the most significant three nutritional factors were further predicted from a polynomial model created from the data obtained from three level factorial design, a Box-Behnken design. Predicted optimal ferrous-ion oxidation rate Q(Fe2+) was recorded to be 0.148 (g Fe2+/l/hr). On verifying the predicted value, an experiment was performed under optimal predicted conditions and showed an actual experimental Q(Fe2+) of 0.152 g/l/hr, which was 2.7% over the predicted value. Our optimized medium formula gave overall five folds increase in ferrous-ion oxidation rates over the previously published data of standard 9-K medium on batch culture of A. ferrooxidans ATCC 23270 with higher mu(max) (hr(-1)) of 0.177 which was achieved within 75 h incubation in shake-flask culture.</p>","PeriodicalId":75388,"journal":{"name":"Acta microbiologica Polonica","volume":"51 3","pages":"225-35"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22247496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phenylalanine ammonia lyase production by gamma irradiated and analog-resistant mutants of Rhodotorula glutinis. γ辐照和抗类似物的粘红曲菌突变体产生苯丙氨酸解氨酶。
Acta microbiologica Polonica Pub Date : 2000-01-01
A I El-Batal, M A Abo-State, A Shihab
{"title":"Phenylalanine ammonia lyase production by gamma irradiated and analog-resistant mutants of Rhodotorula glutinis.","authors":"A I El-Batal,&nbsp;M A Abo-State,&nbsp;A Shihab","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Mutants resistant to phenylalanine analogs (L-tyrosine, p-fluoro-D, L-phenylalanine (PFP) and trans-cinnamic acid) were isolated from a wild type strain of Rhodotorula glutinis A-97 by mutagenic treatment with gamma radiation and screened for phenylalanine ammonia lyase (PAL) production. One such mutant, gammaT11 (resistant to L-tyrosine), exhibited four times the PAL activity of the parent wild strain A-97. Mutant isolate gammaTFP5.6 which was selected as L-tyrosine and PFP resistant isolate, produced inducible PAL activity at levels 5.94-fold higher than the wild-type A-97 and 2.66-fold higher than its parent mutant isolate gammaT5 which was resistant to L-tyrosine. The mutant isolate gammaTC5d which was resistant to L-tyrosine and trans-cinnamic acid, exhibited 3.48 and 1.56-fold increase in PAL activity compared to the parent wild strain A-97 and its parent mutant isolate gammaT5, respectively. Different media have been examined for the induction of PAL.</p>","PeriodicalId":75388,"journal":{"name":"Acta microbiologica Polonica","volume":"49 1","pages":"51-61"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21831066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antimicrobial effect of crude extracts of Nigella sativa on multiple antibiotics-resistant bacteria. 黑草粗提物对多种耐药菌的抑菌作用。
Acta microbiologica Polonica Pub Date : 2000-01-01
N M Morsi
{"title":"Antimicrobial effect of crude extracts of Nigella sativa on multiple antibiotics-resistant bacteria.","authors":"N M Morsi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Different crude extracts of Nigella sativa were tested for antimicrobial effectiveness against different bacterial isolates. These isolates comprised 16 gramnegative and 6 grampositive representatives. They showed multiple resistance against antibiotics, specially the gramnegative ones. Crude extracts of Nigella saliva showed a promising effect against some of the test organisms. The most effective extracts were the crude alkaloid and water extracts. Gramnegative isolates were affected more than the grampositive ones.</p>","PeriodicalId":75388,"journal":{"name":"Acta microbiologica Polonica","volume":"49 1","pages":"63-74"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21831068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immunogenic activity of HSV-1 temperature sensitive mutant's proteins in mono- and polyvalent systems of immunization. 单纯疱疹病毒1型温度敏感突变体蛋白在单价和多价免疫系统中的免疫原活性。
Acta microbiologica Polonica Pub Date : 2000-01-01
A Trzcińska, B Litwińska, M Kańtoch
{"title":"Immunogenic activity of HSV-1 temperature sensitive mutant's proteins in mono- and polyvalent systems of immunization.","authors":"A Trzcińska,&nbsp;B Litwińska,&nbsp;M Kańtoch","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Immunogenic activity of herpes simplex type 1 temperature sensitive mutant's (ts HSV-1 mutant) proteins was tested in two systems: monovalent and polyvalent with other attenuated virus strains (measles and mumps). The guinea pigs were used as animal model. In monovalent system the humoral response in animals infected with ts HSV-1 mutant (1 or 2 doses) was studied and compared to results received for HSV-1 native strain. In polyvalent system the immunological response induced by ts HSV-1 mutant in the presence of RNA virus strains was tested.</p>","PeriodicalId":75388,"journal":{"name":"Acta microbiologica Polonica","volume":"49 2","pages":"101-11"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21917811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Oxidation of elemental sulfur by bacteria and fungi in soil. 土壤中单质硫被细菌和真菌氧化的过程。
Acta microbiologica Polonica Pub Date : 2000-01-01
J Czaban, J Kobus
{"title":"Oxidation of elemental sulfur by bacteria and fungi in soil.","authors":"J Czaban,&nbsp;J Kobus","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Laboratory experiments were used to determine the effects of antibiotics, organic C and CaCO3 amendments of sterile reinoculated soil on S0 oxidation by bacteria and fungi. The rate of S0 oxidation in soil with nystatin added was higher than in soil amended with penicillin + streptomycin. This tells us that bacteria were more efficient than fungi in the S0 oxidation process. It was demonstrated that neutrophilic chemolithotrophs were more efficient in this process than heterotrophs. Glucose introduced to the soil had a negative effect and CaCO3 had a positive effect on S0 oxidation. In soil enriched with glucose the number of chemolithotrophs was very low in comparison with extremely numerous heterotrophic bacteria and fungi. It suggests that the role of heterotrophs in S0 oxidation could be important in habitats rich in organic C, e.g. rhizosphere. In soil containing S0, qualitative changes of fungal communities to genera with higher S0 oxidation ability was also noted. In the presented paper, after comparison of the own results with the data of others concerning the natural soils, the role of various microbial groups in S0 oxidation process in soils is discussed.</p>","PeriodicalId":75388,"journal":{"name":"Acta microbiologica Polonica","volume":"49 2","pages":"135-47"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21917814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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