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Acta microbiologica Polonica最新文献

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Study of solar photosensitization processes on dermatophytic fungi. 皮肤真菌太阳光敏过程的研究。
Acta microbiologica Polonica Pub Date : 2003-01-01
Salama A Ouf, Mahmoud H Abdel-Kader, Hisham A Shokeir, Amira A El-Adly
{"title":"Study of solar photosensitization processes on dermatophytic fungi.","authors":"Salama A Ouf,&nbsp;Mahmoud H Abdel-Kader,&nbsp;Hisham A Shokeir,&nbsp;Amira A El-Adly","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The antifungal activity of solar simulator was evaluated in presence of haematoporphyrin derivative (HPD), methylene blue (MB) and toluidine blue O (TBO) as photosensitizers. Seven dermatophytes were used as test fungi. The solar simulator at fluence rate 400 W/m2 for 30 minutes induced marked inhibition for spore germination of the photosensitized fungi. The rate of inhibition varied according to the fungal species and concentration of the photosensitizer. There was an increase in percentage inhibition of spore germination as the concentration of HPD or MB increased. Complete inhibition for spore germination of Trichophyton. verrucosum, T. mentagrophytes, and Miccrosporum canis was induced when these species were pretreated with 10(-3) M of HPD or MB before irradiation. Epidermophyton floccosum, T. rubrum, M. gypseum and T. violaceum were less sensitive to irradiation when pretreated with HPD or MB. On contrary, the maximum reduction in percentage spore germination was induced at the lowest concentration (10(-7) M) of TBO. The tested dermatophytes were mostly capable of producing different enzymes (keratinase, phosphatases, amylase, lipase). The separate application of radiation or photosensitizer was ineffective or exerted slight inhibition on enzyme production. However, the activity of the enzymes was drastically inhibited when the fungi were irradiated after their treatment with photosensitizer. T. verrucosum and T. mentagrophytes were the most sensitive. In a trail to apply a control measure against dermatomycosis using solar simulator radiation, the results revealed that the radiation was successful in curing the MB-photosensitized guinea pigs, artificially infected with T. verrucosum, T. mentagrophytes or M. canis. The percentage of recovery reached 100% in some treatments.</p>","PeriodicalId":75388,"journal":{"name":"Acta microbiologica Polonica","volume":"52 1","pages":"65-79"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22529167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Siderophore-mediated strategies of iron acquisition by extraintestinal isolates of Enterobacter spp. 肠外分离菌铁载体介导的铁获取策略。
Acta microbiologica Polonica Pub Date : 2003-01-01
Joanna Mokracka, Adam Kaznowski, Małgorzata Szarata, Ewa Kaczmarek
{"title":"Siderophore-mediated strategies of iron acquisition by extraintestinal isolates of Enterobacter spp.","authors":"Joanna Mokracka,&nbsp;Adam Kaznowski,&nbsp;Małgorzata Szarata,&nbsp;Ewa Kaczmarek","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A total of 89 examined Enterobacter isolates belonging to three species: E. cloaceae, E. aerogenes and E. sakazakii, produced iron chelators detected in universal CAS assay. In chemical assays the strains were shown to excrete mostly catecholate (88 strains) and hydroxamate (42 strains) type of siderophores. Forty-one strains produced both catecholate and hydroxamate siderophores whereas one isolate produced only hydroxamate. Besides, the isolates were screened for genes coding for another siderophore: yersiniabactin. The genes for biosynthesis and uptake of yersiniabactin are located on the high-pathogenicity island (HPI) of Yersinia spp. The presence of three marker genes irp1, irp2 and fyuA was estimated by polymerase chain reaction. Two strains: E. aerogenes and E. cloaceae possessed irp1, irp2 and fyuA genes. PCR products of irp1, irp2 and fyuA were of 240, 280 and 780 bp, respectively.</p>","PeriodicalId":75388,"journal":{"name":"Acta microbiologica Polonica","volume":"52 1","pages":"81-6"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22529168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Horizontal DNA transfer between bacteria in the environment. 环境中细菌之间的水平DNA转移。
Acta microbiologica Polonica Pub Date : 2003-01-01
Krystyna I Wolska
{"title":"Horizontal DNA transfer between bacteria in the environment.","authors":"Krystyna I Wolska","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In the environment horizontal DNA transfer between various bacterial species and genera takes place by transformation, transduction, but mainly by conjugation. Conjugation is responsible for the spread of genes coding for antibiotic resistance and xenobiotic degradation. Transfer events are reported in animal, rhizosphere and phylloplane ecosystems and in non polluted and polluted water and soil. Genetic exchange between Bacteria and Archaea is also observed. Evaluation of the extent of interspecies gene transfer is crucial in view of the deliberate release of a variety of unmodified and genetically modified microorganisms into the natural environments.</p>","PeriodicalId":75388,"journal":{"name":"Acta microbiologica Polonica","volume":"52 3","pages":"233-43"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24181509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antimicrobial resistance of Listeria monocytogenes. 单核增生李斯特菌的耐药性。
Acta microbiologica Polonica Pub Date : 2003-01-01
Joanna Poroś-Głuchowska, Zdzisław Markiewicz
{"title":"Antimicrobial resistance of Listeria monocytogenes.","authors":"Joanna Poroś-Głuchowska,&nbsp;Zdzisław Markiewicz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Listeria monocytogenes is an opportunistic pathogen that causes rare but frequently fatal infections, termed listerioses. In general, strains of L. monocytogenes are susceptible to a wide range of antibiotics, except for the cephalosporins, fluorochinolones and fosfomycin (Hof, 1991). The current therapy of choice is a combination of ampicillin and aminoglycoside, usually gentamicin (Lorber, 1997). In cases when it is not possible to use a beta-lactam antibiotic, second-choice therapy involves the use of an association of trimethoprim with a sulfonamide, such as in co-trimoxazole, in which the more active in the combination seems trimethoprim, synergized by the sulfa compound. Other second line agents for listeriosis include erythromycin and vancomycin (Temple and Nahata, 2000). The first strains of L. monocytogenes resistant to antibiotics were reported in 1988 (Poyart-Salmeron et al. 1990) The present paper reviews the current state of affairs with regard to the resistance of L. monocytogenes isolated from food products and clinical material to different antibiotics, with particular emphasis on those used in the therapy of listeriosis.</p>","PeriodicalId":75388,"journal":{"name":"Acta microbiologica Polonica","volume":"52 2","pages":"113-29"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24055556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Purification and characterization of xylanase from a thermophilic Streptomyces sp. K37. 嗜热链霉菌(Streptomyces sp. K37)木聚糖酶的纯化及特性研究。
Acta microbiologica Polonica Pub Date : 2003-01-01
F A Mansour, A A Shereif, M M Nour el-Dein, M I Abou-Dobara, A S Ball
{"title":"Purification and characterization of xylanase from a thermophilic Streptomyces sp. K37.","authors":"F A Mansour,&nbsp;A A Shereif,&nbsp;M M Nour el-Dein,&nbsp;M I Abou-Dobara,&nbsp;A S Ball","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Extracellular xylanase (EC 3.2.1.8) from Streptomyces sp. K37 was purified 33.53 by ultrafiltration and cation exchange chromatography followed by gel filtration chromatography. The optimum pH and temperature for purified xylanase were found to be pH 6.0 and 60 degrees C. The Km and V(max) values of the purified xylanase were 15.4 mg ml(-1) and 0.67 micromole reducing sugar min(-1) ml(-1). High performance liquid chromatography (HPLC) gel filtration of the purified xylanase eluted xylanase activity as a peak corresponding to the molecular weight of about 24.3 kDa while the molecular weight determined by SDS-PAGE was found to be 26.4 kDa. The purified xylanase of Streptomyces sp. K37 was found to be endoxylanase and non arabinose liberating enzyme and was highly glycosylated (73.97%).</p>","PeriodicalId":75388,"journal":{"name":"Acta microbiologica Polonica","volume":"52 2","pages":"159-72"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24055560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Relationship between soil microbial diversity and bioremediation process at an oil refinery. 炼油厂土壤微生物多样性与生物修复过程的关系
Acta microbiologica Polonica Pub Date : 2003-01-01
Grazyna Płaza, Krzysztof Ulfig, Robin L Brigmon
{"title":"Relationship between soil microbial diversity and bioremediation process at an oil refinery.","authors":"Grazyna Płaza,&nbsp;Krzysztof Ulfig,&nbsp;Robin L Brigmon","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Microbial diversity in hydrocarbon-contaminated soil was characterized during a bioremediation project at an oil refinery. The project consisted of isolation and cultivation of microbes on laboratory media and the subsequent characterization of pure isolates. In a lagoon at the Czechowice Oil Refinery, Poland, a biopile with actively and passively aerated sections was constructed and has been operated since 1997. The bioremediation process has been continuously monitored by physical, chemical, and microbiological methods. One hundred and forty nine bacterial and fungal strains were isolated from site soils by standard procedures. Analysis of cultivable microorganisms revealed a diverse microbial population within the cultured isolates. Among isolated strains, Pseudomonas and Chryseomonas genera predominated in the bacterial population while Candida, Fusarium, and Trichophyton dominated the fungal population. This paper describes the application of traditional microbiological methods (plating and microscopic methods) to evaluate cultivable microbial diversity in bioremediated soil.</p>","PeriodicalId":75388,"journal":{"name":"Acta microbiologica Polonica","volume":"52 2","pages":"173-82"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24055561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of health value of fats and oils. 油脂健康价值的评价。
Acta microbiologica Polonica Pub Date : 2003-01-01
Karol Bal, Jarosław Balas, Imarek Daniewski, Agnieszka Filipek, Bohdan Jacórzyński, Eugenia Mielniczuk, Małgorzata Pawlicka, Katarzyna Swiderska
{"title":"Evaluation of health value of fats and oils.","authors":"Karol Bal,&nbsp;Jarosław Balas,&nbsp;Imarek Daniewski,&nbsp;Agnieszka Filipek,&nbsp;Bohdan Jacórzyński,&nbsp;Eugenia Mielniczuk,&nbsp;Małgorzata Pawlicka,&nbsp;Katarzyna Swiderska","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The fat content and fatty acid composition (saturated, mono- and polyunsaturated in cis and trans configuration) in selected food products was determined. From over 2000 products investigated in recent years, results are given for some soft and hard margarines, oils and butter as well as butter-like products. It has been found that food products are distinguished by very high diversification with regard to fat content, in terms of both quantity and quality. This differentiation was particularly evident in the groups of margarines, mixed fats and confectioner's products.</p>","PeriodicalId":75388,"journal":{"name":"Acta microbiologica Polonica","volume":"52 Suppl ","pages":"7-15"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24447120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Trans-unsaturated fatty acids and acrylamide in food as potential atherosclerosis progression factors. Based on own studies. 食物中的反式不饱和脂肪酸和丙烯酰胺是潜在的动脉粥样硬化进展因素。基于自己的研究。
Acta microbiologica Polonica Pub Date : 2003-01-01
Marek Naruszewicz, Marek Daniewski, Grazyna Nowicka, Małgorzata Kozłowska-Wojciechowska
{"title":"Trans-unsaturated fatty acids and acrylamide in food as potential atherosclerosis progression factors. Based on own studies.","authors":"Marek Naruszewicz,&nbsp;Marek Daniewski,&nbsp;Grazyna Nowicka,&nbsp;Małgorzata Kozłowska-Wojciechowska","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Atherosclerosis is a chronic pathological process and it is generelly accepted that lipids, coagulation and inflammatory factors play an important role in its development. Environmental factors such as bed diet and cigarette smoking strongly stimulate initation and progression of atherosclerotic changes in the artery wall. It has been recognized that deeply processed food may be a source of various factors potentiating processes related to atherosclerosis development among which inflammatory processes are of great importance. The aim of our studies was to find out if the trans-unsaturated fatty acids as well as acrylamide present in foods have the potential to provoke pro-inflammatory states in the body and enhance atherosclerosis risk. The results of our in vitro studies have shown that trans fatty acids cause a significant increase in secretion of reactive oxygen species, interleukin-6, tumor necrosis factor a and metalloproteinase-9, and enhance apoptosis. It indicates that in vivo trans-fatty acids may distroy the endothelium integrity and cause plaque rupture. Our in vivo studies in the group of healthy volunteers have shown that the consumption of potato chips rich in acrylamide cause the significant increase in plasma C-reactive protein and homocysteine concentrations. Enhanced CRP and HCY levels are accepted markers of enhanced atherosclerosis risk.</p>","PeriodicalId":75388,"journal":{"name":"Acta microbiologica Polonica","volume":"52 Suppl ","pages":"75-81"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24447609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Synergistic action of photosensitizers and normal human serum in a bactericidal process. I. Effect of chlorophylls. 光敏剂与正常人血清在杀菌过程中的协同作用。1 .叶绿素的作用。
Acta microbiologica Polonica Pub Date : 2003-01-01
Andrzej Jankowski, Stanisław Jankowski, Agnieszka Mirończyk
{"title":"Synergistic action of photosensitizers and normal human serum in a bactericidal process. I. Effect of chlorophylls.","authors":"Andrzej Jankowski,&nbsp;Stanisław Jankowski,&nbsp;Agnieszka Mirończyk","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Susceptibility of some Gram-negative strains against the bactericidal action of normal human serum (NHS) and of chlorophyll, which induces production of reactive oxygen species by light, was studied. A synergistic bactericidal activity of NHS and chlorophyll against E. coli K1 and Shigella flexneri strains was observed.</p>","PeriodicalId":75388,"journal":{"name":"Acta microbiologica Polonica","volume":"52 4","pages":"373-8"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24478371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The molecular characterisation of the extended spectrum beta-lactamase (ESBL) producing strain of Salmonella enterica serovar Mbandaka isolated in Poland. 波兰分离的Mbandaka肠炎沙门氏菌血清型扩展谱β -内酰胺酶(ESBL)产生菌株的分子特征。
Acta microbiologica Polonica Pub Date : 2003-01-01
Rafał Gierczyński, Jolanta Szych, Waldemar Rastawicki, Marek Jagielski
{"title":"The molecular characterisation of the extended spectrum beta-lactamase (ESBL) producing strain of Salmonella enterica serovar Mbandaka isolated in Poland.","authors":"Rafał Gierczyński,&nbsp;Jolanta Szych,&nbsp;Waldemar Rastawicki,&nbsp;Marek Jagielski","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The molecular attributes of the first ESBL producing isolate 267/99 of Salmonella enterica serovar Mbandaka isolated in 1999 in Poland were characterised. Tested strain produced CTX-M-3 and TEM-1 beta-lactamases, encoded by genes localised on the about 80 kb self-transferable plasmid, harbouring the 2 kb class 1 integron carrying aadA2 gene.</p>","PeriodicalId":75388,"journal":{"name":"Acta microbiologica Polonica","volume":"52 2","pages":"183-90"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24056018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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