Acta cientifica venezolana最新文献_第8页

Heritability and correlation among some selected morphological traits and their relationship with fall armyworm damage in sweet corn. 甜玉米若干形态性状的遗传力、相关性及其与秋粘虫危害的关系。

Acta cientifica venezolana Pub Date : 2002-01-01

Ramón A Rea, Clarence E Watson, William P Williams, Frank M Davis

{"title":"Heritability and correlation among some selected morphological traits and their relationship with fall armyworm damage in sweet corn.","authors":"Ramón A Rea, Clarence E Watson, William P Williams, Frank M Davis","doi":"","DOIUrl":"","url":null,"abstract":"Fall armyworm [Spodoptera frugiperda J. E. Smith] (FAW) is a serious pest in field corn and sweet corn [Zea mays L.] in many parts of the world. Sweet corn germplasm with effective levels of resistance to damage by the fall armyworm is needed to transfer resistance to commercial hybrids. The objectives of this study were to estimate heritability and to estimate the correlation among some selected morphological traits and their relationship with FAW damage. Seven shrunken-2 (sh2) inbred lines and four commercial sh2 hybrids of sweet corn were crossed to Mp708, a FAW-resistant field corn line. The F2 populations were subdivided with one half being selected for the sh2 trait and the other half was left unselected. Parent, F2, and F3 populations were artificially infested with FAW and evaluated for leaf damage caused by FAW. Heritability estimates for FAW resistance ranged from 0.22 to 0.61 depending on method of estimation used. The highest correlations occurred between silk color and anther color (0.70) and silk color and glume color (0.49). There were no consistent correlations of most morphological traits with FAW damage. A linkage between white silk and shrunken-2 was observed.","PeriodicalId":75378,"journal":{"name":"Acta cientifica venezolana","volume":"53 1","pages":"66-9"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21978028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The glands of the dorso-distal mucosa of the toad Bufo marinus (Amphibia: Anura) tongue: histology and ultrastructure ]. [蟾蜍舌背远端粘膜腺体(两栖类:无尾目):组织学和超微结构]。

Acta cientifica venezolana Pub Date : 2002-01-01

Miren González-Elorriaga

引用次数: 0

[Diatoms from Unare and Píritu Lagoons, Anzoátegui state, Venezuela. Subclass Bacillariophycidae D. G. Mann ]. [来自委内瑞拉Anzoátegui州的Unare和Píritu泻湖的硅藻。硅藻亚纲[D. G. Mann]。

Acta cientifica venezolana Pub Date : 2002-01-01

Sonia S Subero-Pino, José R Diaz-Ramos, Isaac G Sanchez-Suarez, Elvira Ferraz-Reyes

引用次数: 0

Utilization of bee (Apis mellifera) honey for vinegar production at laboratory scale. 在实验室规模上利用蜜蜂蜂蜜生产醋。

Acta cientifica venezolana Pub Date : 2000-06-30 DOI: 10.5380/CEP.V18I1.1123

E. C. Ilha, E. Sant’Anna, R. C. Torres, A. C. Porto, E. M. Meinert

{"title":"Utilization of bee (Apis mellifera) honey for vinegar production at laboratory scale.","authors":"E. C. Ilha, E. Sant’Anna, R. C. Torres, A. C. Porto, E. M. Meinert","doi":"10.5380/CEP.V18I1.1123","DOIUrl":"https://doi.org/10.5380/CEP.V18I1.1123","url":null,"abstract":"Vinegar was obtained from bee (Apis mellifera) honey. The wort was prepared by diluting honey in distilled water to 21% total solids and by adding ammonium sulfate and ammonium phosphate. Saccharomyces cerevisiae was inoculated to the wort (4 g/L). Ethanol production was carried out at room temperature during 84 hours. In this study, 1 Kg of honey yielded about 5 L of wine, containing 8% alcohol (v/v), from a wort with 17.11% total sugars (w/v). The efficiency of the alcoholic fermentation was 81.34%. The acetic fermentation with an inoculum of mixed acetic microorganisms was performed by quick process in a 15 L vertical fermenter. This resulted in a vinegar containing up to 9% of acetic acid (w/v) and about 1% of alcohol (v/v). The acetic fermentation yielded between 91.24 and 97.21%. Approximately 5 L of honey vinegar with 9% acetic acid (w/v) were obtained from 1 Kg of bee honey. All attributes of honey vinegar showed acceptability index over 70%: 95.37% for appearance, 94.81% for color, 79.07% for odor and 75.56% for flavor, indicating it would show good consumer acceptability.","PeriodicalId":75378,"journal":{"name":"Acta cientifica venezolana","volume":"51 4 1","pages":"231-5"},"PeriodicalIF":0.0,"publicationDate":"2000-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5380/CEP.V18I1.1123","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70974011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

[Multidrug or pleiotropic resistance]. [多药或多效耐药]。

Acta cientifica venezolana Pub Date : 2000-01-01

F Arvelo, E Merentes, C Cotte

引用次数: 0

Disruption of the intestinal barrier and bacterial translocation in an experimental model of intestinal obstruction. 肠梗阻实验模型中肠屏障的破坏和细菌移位。

Acta cientifica venezolana Pub Date : 2000-01-01

R Antequera, A Bretaña, A Cirac, A Brito, M A Romera, R Zapata

{"title":"Disruption of the intestinal barrier and bacterial translocation in an experimental model of intestinal obstruction.","authors":"R Antequera, A Bretaña, A Cirac, A Brito, M A Romera, R Zapata","doi":"","DOIUrl":"","url":null,"abstract":"Clinical evidence and the use of experimental models in laboratory animals indicate that the intestine is a reservoir of microorganisms that can cause systemic infection in the human. The purpose of this work was to study the possible effect of intestinal obstruction (IO) on the mechanical and chemical barriers that bring protection against microorganisms crossing from the intestinal lumen towards the systemic tissues. We demonstrated that 24 hours after IO, histological and ultrastructural alterations do occur, seriously compromising the structure of the intestinal barrier in 100% of the studied animals. Likewise, it was observed that during the same period, microorganisms translocation from intestine to the peritoneal cavity and liver (100 and 80% respectively) occurred. The lungs were spared. Changes observed in the intestinal epithelium are related to a process similar to that produced by intestinal ischemia: mitochondrial destruction, with subsequent decrease of its capacity to supply energy and to preserve the equilibrium and structure of the intestinal epithelium. We propose that translocation of enteric bacteria may be the cause of the infection that brings about the death a significant group of animals at 48 hours (27%) and 72 hours (33%) post-IO.","PeriodicalId":75378,"journal":{"name":"Acta cientifica venezolana","volume":"51 1","pages":"18-26"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21810240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Occupational exposure and health effects of metallic mercury among dentists and dental assistants: a preliminary study. Valencia, Venezuela; 1998]. [牙医和牙科助理对金属汞的职业接触和健康影响:初步研究。瓦伦西亚、委内瑞拉;1998]。

Acta cientifica venezolana Pub Date : 2000-01-01

M Rojas, H Guevara, R Rincón, M Rodríguez, C Olivet

{"title":"[Occupational exposure and health effects of metallic mercury among dentists and dental assistants: a preliminary study. Valencia, Venezuela; 1998].","authors":"M Rojas, H Guevara, R Rincón, M Rodríguez, C Olivet","doi":"","DOIUrl":"","url":null,"abstract":"The aim of this investigation was to establish mercury (Hg) health effects on dentists and dental assistants, its relationship with exposure conditions and the potential renal damage Hg-related. The total population was 66 people, with a sample of 37 (56%), 22 dentists (59.5%, 19 male, 3 female) and 15 dental assistants (40.5%, all female). This was accomplished by an interview, Hg in urine (Hg-U) and N-acetyl-B-D-glucosaminidase activity in urine (NAG-U). Average values of Hg-U for dentists were 22.4 +/- 6.4 micrograms/g creatinine and 22.2 +/- 6.1 micrograms/g creatinine for dental assistants NAG-U average values were 2.9 +/- 3 U/L and 5.2 +/- 8.1 U/L respectively. There were no statistically significant differences between these averages (p > 0.05). There was no correlation between the quantity of amalgam prepared and working hours with Hg-U and NAG-U. Most frequent symptoms referred by dentists were: irritability (54.5%), cephalalgia (45.4%), arthralgias (40.9%), and the ones more referred by assistants were arthralgias (53.3%), irritability (46.7%) and cephalalgia (46.7%). It was not found a significative risk of having them among these groups. There is a need for further investigations including environmental monitoring of Hg, clinical evaluation and neurobehavioural tests to detect early effects. It is important to enforce personal safety measures to control the exposure.","PeriodicalId":75378,"journal":{"name":"Acta cientifica venezolana","volume":"51 1","pages":"32-8"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21810242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Biodegradation of Venezuelan woods in field stake tests in the western lowlands]. [在西部低地实地木桩试验中委内瑞拉木材的生物降解]。

Acta cientifica venezolana Pub Date : 2000-01-01

O Encinas

引用次数: 0

Construction of a cassette for cloning and analysis of replicons. 用于复制子克隆和分析的盒式结构。

Acta cientifica venezolana Pub Date : 2000-01-01

G Alonso, J Campos, I Bruzual, V Rodríquez Lemoine

{"title":"Construction of a cassette for cloning and analysis of replicons.","authors":"G Alonso, J Campos, I Bruzual, V Rodríquez Lemoine","doi":"","DOIUrl":"","url":null,"abstract":"The aim of this work was the construction of a cassette, i.e., a non-replicative molecule formed by linkage of an antibiotic resistance gene and a multiple cloning site. This cassette would allow the cloning and analysis of a wide range of replicons. The aac(6')-lc amikacin gene was isolated and ligated to the multiple cloning site of the pUC18 vector. This construction was HindIII digested and cloned in the HindIII site of the vector. The resulting pHJ13 clone conferred to the recipient cells the ability to grow in presence of amikacin (cassette marker) and ampicillin (vector gene). By restriction analysis, the cassette orientation was established. Cassette versatility is provided by the presence of the unaltered multiple cloning site segment, and also because it allows sequencing of any replication origin inserted. Cassette functionality was demonstrated by ligation to a replicative region of H plasmid pHH1457. Presence of the ori region from pHH1457 and the aac(6')-lc gene was confirmed in E. coli transformed clones. The incompatibility properties of the pHH1457 and its capability to replicate in a Poll defective strain were preserved in the pHJII14 construct. Currently, the amikacin cassette is being used in the characterization of H Complex plasmids.","PeriodicalId":75378,"journal":{"name":"Acta cientifica venezolana","volume":"51 1","pages":"4-9"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21810239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Measurement of serum amiodarone by protein precipitation followed by high performance liquid chromatography with ultraviolet detection]. [蛋白质沉淀-高效液相色谱-紫外检测法测定血清胺碘酮]。

Acta cientifica venezolana Pub Date : 2000-01-01

M Valencia, V Zapata, A Bider, M Pérez-González, V Lamanna

{"title":"[Measurement of serum amiodarone by protein precipitation followed by high performance liquid chromatography with ultraviolet detection].","authors":"M Valencia, V Zapata, A Bider, M Pérez-González, V Lamanna","doi":"","DOIUrl":"","url":null,"abstract":"Our aim was to develop a quantitative method for serum amiodarone measurement using high performance liquid chromatography with ultraviolet photometric detection. We studied previous reports in the literature in order to obtain a simpler method to be used routinely in our TDM unit. Sample preparation was based on protein precipitation adding 2 parts of acetonitrile to 1 part of serum, followed by vortex-agitation for 45 s, incubation at 24 degrees C for 5 min, and centrifugation at 6000 x g for 2 min. Twenty microliters of the supernatant was directly injected into the chromatographic system. A microBondapak CN RP column (3.9 x 150 mm) at 45 degrees C, with a mobile phase consisting of KH2PO4 10 mM/methanol/acetonitrile (40:37:23 v/v/v), pH 3.5, were used. Eluting with a flow rate of 0.6 mL/mm the retention time of amiodarone was approximately 4.9 min. Detection was performed at 242 nm and the quantification was made by peak height comparison with external standards. The mass/response ratio is linear (r2 > 0.99) within a mass range of 2.96 to 18,930 ng of injected amiodarone, which exceeds the requirements for the monitoring of serum levels (0.3 to 6.0 micrograms/mL). Sample storage should be done with acetonitrile-extracted sera at -16 degrees C to avoid degradation. The method is very efficient, linear, sensitive and specific but it also simpler and cheaper than others reported in the literature.","PeriodicalId":75378,"journal":{"name":"Acta cientifica venezolana","volume":"51 1","pages":"53-60"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21810245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0