Zoonoses (Burlington, Mass.)最新文献

OROPOUCHE VIRUS: MORE QUESTIONS THAN ANSWERS. 奥罗普切病毒：问题多于答案。

Zoonoses (Burlington, Mass.) Pub Date : 2024-01-01 Epub Date: 2024-07-30 DOI: 10.15212/zoonoses-2024-0006

Eduardo Jurado-Cobena

引用次数: 0

Global Infectious Diseases in December 2022: Monthly Analysis 2022年12月全球传染病:月度分析

Zoonoses (Burlington, Mass.) Pub Date : 2023-01-28 DOI: 10.15212/zoonoses-2022-1005

Qi Xiang, Taihan Li, Minjing He, Shuqiong Zhang, Yi Luo, Jiazhen Zou, Guodan Li, Qun Su, Shiping He, Wenjin Yu, Dayong Gu

引用次数: 0

Corrigendum to: Pattern Recognition Receptors in Innate Immunity to Obligate Intracellular Bacteria. 对专性细胞内细菌先天免疫中的模式识别受体的更正。

Zoonoses (Burlington, Mass.) Pub Date : 2023-01-03 DOI: 10.15212/zoonoses-2023-1003

James R Fisher, Zachary D Chroust, Florence Onyoni, Lynn Soong

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The Coordinating Research on Emerging Arboviral Threats Encompassing the Neotropics (CREATE-NEO). 新热带地区新出现的虫媒病毒威胁协调研究（CREATE-NEO）。

Zoonoses (Burlington, Mass.) Pub Date : 2023-01-01 Epub Date: 2023-04-18 DOI: 10.15212/zoonoses-2022-0047

Nikos Vasilakis, Kathryn A Hanley

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NAN342K Mutation Enhances the Pathogenicity of Influenza B Virus in Mice NAN342K突变增强乙型流感病毒对小鼠的致病性

Zoonoses (Burlington, Mass.) Pub Date : 2023-01-01 DOI: 10.15212/zoonoses-2023-0013

Qi Chen, Xiaohao Xu, Min Tan, Lei Yang, Dayan Wang, Yuelong Shu, Wenfei Zhu

{"title":"NAN342K Mutation Enhances the Pathogenicity of Influenza B Virus in Mice","authors":"Qi Chen, Xiaohao Xu, Min Tan, Lei Yang, Dayan Wang, Yuelong Shu, Wenfei Zhu","doi":"10.15212/zoonoses-2023-0013","DOIUrl":"https://doi.org/10.15212/zoonoses-2023-0013","url":null,"abstract":"Objective: Influenza B virus is a significant respiratory pathogen responsible for seasonal influenza. In recent years the B/Yamagata lineage has demonstrated a rapid increase, predominantly featuring the neuraminidase (NA) N342K mutation. This study determined the impact of the NA N342K mutation on the pathogenicity of influenza B virus and elucidate the underlying mechanisms. Methods: Gene fragments with specific mutations were generated using site-directed mutagenesis PCR, resulting in recombinant viruses (rAH127 and rAH127/NA N342K ). C57BL/6 mice were infected to evaluate the impact of amino acid mutations on virus pathogenicity. Body weight, survival rate, virus replication, and lung pathology were compared among the groups. NA enzyme activity was assessed to determine the mechanisms underlying the effects of amino acid mutations on the pathogenicity of influenza B virus. Results: The NA N342K mutant virus exhibited significantly increased NA enzyme activity (3.19-fold) and viral replication capacity in MDCK cells (6.76-fold) compared to wild-type virus. These changes led to enhanced pathogenicity in mice, characterized by severe weight loss, increased mortality, and heightened lung tissue inflammation. Conclusions: The NA N342K mutation likely enhances virus replication and pathogenicity by increasing NA enzyme activity. These findings contribute to understanding the molecular mechanisms underlying influenza B virus pathogenicity and have implications for targeted therapeutic strategies.","PeriodicalId":75363,"journal":{"name":"Zoonoses (Burlington, Mass.)","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135611498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global Infectious Diseases in August 2023: A Monthly Analysis 2023年8月全球传染病:月度分析

Zoonoses (Burlington, Mass.) Pub Date : 2023-01-01 DOI: 10.15212/zoonoses-2023-1009

Jiawen Huang, Yi Luo, Jiazhen Zou, Yufan Wu, Yinfu Sun, Qi Xiang, Minjing He, Shuqiong Zhang, Wenjin Yu, Qun Su, Taihan Li, Dayong Gu

引用次数: 0

Rapid and Visual Detection of Monkey B Virus Based on Recombinase Polymerase Amplification 基于重组酶聚合酶扩增的猴B型病毒快速视觉检测

Zoonoses (Burlington, Mass.) Pub Date : 2023-01-01 DOI: 10.15212/zoonoses-2023-0031

Xinlan Chen, Chenchen Liu, Fangxu Li, Junhui Zhou, Zanheng Huang, Haili Zhang, Hualei Wang, Pei Huang, Zengguo Cao, Sandra Chiu

{"title":"Rapid and Visual Detection of Monkey B Virus Based on Recombinase Polymerase Amplification","authors":"Xinlan Chen, Chenchen Liu, Fangxu Li, Junhui Zhou, Zanheng Huang, Haili Zhang, Hualei Wang, Pei Huang, Zengguo Cao, Sandra Chiu","doi":"10.15212/zoonoses-2023-0031","DOIUrl":"https://doi.org/10.15212/zoonoses-2023-0031","url":null,"abstract":"Objective: Monkey B virus (BV) infection in humans and other macaque species has a mortality rate of approximately 80%. Because BV infects humans through bites, scratches, and other injuries inflicted by macaques, the simple and rapid diagnosis of BV in field laboratories is of great importance to protect veterinarians, laboratory researchers, and support personnels from the threat of infection. Methods: Two recombinase polymerase amplification (RPA) assays with a closed vertical flow (VF) visualization strip (RPA-VF-UL27 and RPA-VF-US6) were developed that target two conserved genes combined with a one-off, closed visualization strip device. We compared the sensitivities and specificities of the two assays after optimization of the reaction conditions. The performance of RPA-VF-US6 at room temperature was determined to evaluate its potential in point-of-care (POC) testing. Result: RPA-VF-US6 specifically detected the positive plasmid control (rather than nucleic acids of herpesviruses) with a detection limit of 28 copies, while RPA-VF-UL27 had cross-reactivity with HSV-1, but even 3.4 copies of plasmid standards were readout by this assay. Moreover, RPA-VF-US6 had excellent performance at room temperature (the detection limit was 2,800 plasmid copies), indicating the potential of RPA-VF-US6 in POC testing. Conclusion: We developed two RPA assays for BV visualization diagnosis. RPA-VF-US6 is a simple, rapid, and specific detection method for BV. The entire reaction can be performed at a constant temperature within 30 min, suggesting the potential of RPA-VF-US6 for POC testing in field laboratories without sophisticated instruments.","PeriodicalId":75363,"journal":{"name":"Zoonoses (Burlington, Mass.)","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135650555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Candida haemulonii Species Complex: Emerging Fungal Pathogens of the Metschnikowiaceae Clade 假丝酵母haemulonii种复合体:假丝酵母科新出现的真菌病原体

Zoonoses (Burlington, Mass.) Pub Date : 2023-01-01 DOI: 10.15212/zoonoses-2023-0021

Chengjun Cao, Jian Bing, Guojian Liao, Clarissa J Nobile, Guanghua Huang

引用次数: 0

The Threat of Cholera in Africa 非洲霍乱的威胁

Zoonoses (Burlington, Mass.) Pub Date : 2023-01-01 DOI: 10.15212/zoonoses-2023-0027

Gashaw Adane Erkyihun, Negga Asamene, Ashagrie Zewdu Woldegiorgis

{"title":"The Threat of Cholera in Africa","authors":"Gashaw Adane Erkyihun, Negga Asamene, Ashagrie Zewdu Woldegiorgis","doi":"10.15212/zoonoses-2023-0027","DOIUrl":"https://doi.org/10.15212/zoonoses-2023-0027","url":null,"abstract":"A multi-country cholera outbreak has occurred in several developing countries in Africa, Asia and Oceania since mid-2021. The current multi-country cholera outbreak has spread primarily in Africa. As of February 2023, cholera cases have been reported in approximately 25 countries, 15 (60%) of which are in sub-Saharan Africa. According to the World Health Organization, 547,626 confirmed cases and 4,927 total deaths were recorded until August 2023. Of these, approximately 33.68% cases (184,474) and 64.2% deaths (3,165) occurred in African countries, and the number may increase in the coming months. Although various organizations and local administrations have made response efforts, the response capacity for multiple outbreaks is insufficient, and the outbreak has been exacerbated by a global lack of resources, cholera vaccine shortages, climatic effects, a lack of supplies and the occurrence of other health emergencies. Herein, the rapid review method was used, with emphasis on available and recent global information found in Google Scholar, PubMed and data publicly available from websites. This review article provides current alert information regarding the broad spread and upsurge characteristics of the ongoing cholera outbreak.","PeriodicalId":75363,"journal":{"name":"Zoonoses (Burlington, Mass.)","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134884231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing the Use of Solid-Phase Reversible Immobilization Beads for High-Throughput Full-Length 16S rDNA Sequencing Library Construction 高通量16S rDNA全长测序文库构建中固相可逆固定珠的优化使用

Zoonoses (Burlington, Mass.) Pub Date : 2023-01-01 DOI: 10.15212/zoonoses-2023-0007

Yinmei Li, Ziqiang He, Mimi Kong, Dong Jin

{"title":"Optimizing the Use of Solid-Phase Reversible Immobilization Beads for High-Throughput Full-Length 16S rDNA Sequencing Library Construction","authors":"Yinmei Li, Ziqiang He, Mimi Kong, Dong Jin","doi":"10.15212/zoonoses-2023-0007","DOIUrl":"https://doi.org/10.15212/zoonoses-2023-0007","url":null,"abstract":"Objective: Solid-phase reversible immobilization (SPRI) beads are widely used for high-throughput sequencing library construction to purify and recover nucleic acids. This research was aimed at investigating the effects of SPRI bead ratio, incubation time, and elution time on nucleic acid recovery during full-length 16S rDNA high-throughput sequencing library construction. Methods: The effects of different SPRI bead ratios, incubation times, and elution times were compared for three different initial sample amounts. An L9(3 3 ) orthogonal experiment was designed to determine the optimal combination of these factors. Results: The incubation time of three factors including SPRI beads ratio, incubation time, and elution time had a statistically significant effect on the recovery rate for the initial sample amount of 1500 ng and 3000 ng. The orthogonal experiment results indicated that incubation time had the greatest impact among the three factors. Conclusion: Incubation time significantly influences recovery rate in full-length 16S rDNA high-throughput sequencing library construction. The use of 0.8× SPRI beads, 15 minutes of incubation, and 10 minutes of elution resulted in the highest recovery rate. SPRI beads offer a viable method for recovering full-length 16S rDNA amplicons.","PeriodicalId":75363,"journal":{"name":"Zoonoses (Burlington, Mass.)","volume":"2012 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136306087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0