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High quality genome assembly and annotation (v1) of the eukaryotic terrestrial microalga Coccomyxa viridis SAG 216-4 真核陆生微型藻类 Coccomyxa viridis SAG 216-4 的高质量基因组组装和注释 (v1)
Peer community journal Pub Date : 2024-08-08 DOI: 10.24072/pcjournal.447
Anton Kraege, Edgar A. Chavarro-Carrero, Nadège Guiglielmoni, Eva Schnell, Joseph Kirangwa, Stefanie Heilmann-Heimbach, Kerstin Becker, Karl Köhrer, Philipp H. Schiffer, Bart P. H. J. Thomma, Hanna Rovenich
{"title":"High quality genome assembly and annotation (v1) of the eukaryotic terrestrial microalga Coccomyxa viridis SAG 216-4","authors":"Anton Kraege, Edgar A. Chavarro-Carrero, Nadège Guiglielmoni, Eva Schnell, Joseph Kirangwa, Stefanie Heilmann-Heimbach, Kerstin Becker, Karl Köhrer, Philipp H. Schiffer, Bart P. H. J. Thomma, Hanna Rovenich","doi":"10.24072/pcjournal.447","DOIUrl":"https://doi.org/10.24072/pcjournal.447","url":null,"abstract":"Unicellular green algae of the genus Coccomyxa are recognized for their worldwide distribution and","PeriodicalId":74413,"journal":{"name":"Peer community journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141929246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ecotoxicity of lanthanides to Daphnia magna: insights from elemental behavior and speciation in a standardized test medium 镧系元素对大型蚤的生态毒性:从标准化测试介质中的元素行为和种类中获得的启示
Peer community journal Pub Date : 2024-07-19 DOI: 10.24072/pcjournal.440
D. Vignati, Loïc A Martin, L. Poirier, A. Zalouk‐Vergnoux, C. Fouque, Clément Bojic, Christophe Hissler, Carole Cossu-Leguille
{"title":"Ecotoxicity of lanthanides to Daphnia magna: insights from elemental behavior and speciation in a standardized test medium","authors":"D. Vignati, Loïc A Martin, L. Poirier, A. Zalouk‐Vergnoux, C. Fouque, Clément Bojic, Christophe Hissler, Carole Cossu-Leguille","doi":"10.24072/pcjournal.440","DOIUrl":"https://doi.org/10.24072/pcjournal.440","url":null,"abstract":"Lanthanides (LNs) are a group of 15 elements with steadily increasing economical importance due to their multiple uses in technologies essential for sustainable ecological, digital and energetic transitions. Although knowledge on LN ecotoxicology has greatly improved over the last decade, uncertainty persists with regard to their actual hazard and risk in freshwater environments. In particular, only limited information is available on i) the actual relationships between LN speciation vs . ecotoxicological responses in standardized laboratory tests and ii) the existence of regular and predictable patterns in LN ecotoxicity (expressed as e.g. , EC 50 ) along the LN series. The present paper provides the first report on the ecotoxicity of all lanthanides (except Pm) for the freshwater crustacean Daphnia magna along with an unprecedented level of detail on LN speciation in the exposure medium. Experimental data show that exposure concentrations can decrease by up to 95 % over the test duration, with the percentage decrease being inversely related with LN atomic mass. Thermodynamic speciation calculations confirm the possible formation of insoluble species, mainly LN carbonates. However, the corresponding theoretical solubility limits do not fully agree with measured concentrations at the end of the tests. Experimental verification of exposure concentrations (as a minimum at the beginning and end of laboratory tests) remains therefore mandatory to reach proper conclusions as to the ecotoxicity of each LN. A decreasing trend in ecotoxicity can actually be observed along the LN series when temporal changes in the exposure concentrations are properly accounted for. However, this trend remains dependent on exposure time and selected exposure metrics. This and other caveats must be considered in future research to reach a community-based consensus for the proper hazard and risk assessment of LN towards daphnids and other aquatic organisms.","PeriodicalId":74413,"journal":{"name":"Peer community journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141821080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
T7 DNA polymerase treatment improves quantitative sequencing of both double-stranded and single-stranded DNA viruses T7 DNA 聚合酶处理可提高双链和单链 DNA 病毒的定量测序能力
Peer community journal Pub Date : 2024-07-11 DOI: 10.24072/pcjournal.437
Maud Billaud, Ilias Theodorou, Quentin Lamy-Besnier, Shiraz A. Shah, François Lecointe, Luisa De Sordi, Marianne De Paepe, Marie-Agnès Petit
{"title":"T7 DNA polymerase treatment improves quantitative sequencing of both double-stranded and single-stranded DNA viruses","authors":"Maud Billaud, Ilias Theodorou, Quentin Lamy-Besnier, Shiraz A. Shah, François Lecointe, Luisa De Sordi, Marianne De Paepe, Marie-Agnès Petit","doi":"10.24072/pcjournal.437","DOIUrl":"https://doi.org/10.24072/pcjournal.437","url":null,"abstract":"Bulk microbiome, as well as virome-enriched shotgun sequencing only reveals the double-stranded DNA (dsDNA) content of a given sample, unless specific treatments are applied. However, genomes of viruses often consist of a circular single-stranded DNA (ssDNA) molecule. Pre-treatment and amplification of DNA using the multiple displacement amplifica-tion (MDA) method enables conversion of ssDNA to dsDNA, but this process can lead to over-representation of these circular ssDNA genomes. A more recent alternative permits to bypass the amplification","PeriodicalId":74413,"journal":{"name":"Peer community journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141656268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differences in specificity, development time and virulence between two acanthocephalan parasites, infecting two cryptic species of Gammarus fossarum 两种棘头蚴寄生虫在特异性、发育时间和毒力方面的差异,它们感染了两种隐蔽的福寿螺
Peer community journal Pub Date : 2024-07-08 DOI: 10.24072/pcjournal.434
Alexandre Bauer, Lucie Develay Nguyen, S. Motreuil, Maria Teixeira, Nelly Debrosse, Thierry Rigaud
{"title":"Differences in specificity, development time and virulence between two acanthocephalan parasites, infecting two cryptic species of Gammarus fossarum","authors":"Alexandre Bauer, Lucie Develay Nguyen, S. Motreuil, Maria Teixeira, Nelly Debrosse, Thierry Rigaud","doi":"10.24072/pcjournal.434","DOIUrl":"https://doi.org/10.24072/pcjournal.434","url":null,"abstract":"","PeriodicalId":74413,"journal":{"name":"Peer community journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141668995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multiproxy analysis exploring patterns of diet and disease in dental calculus and skeletal remains from a 19th century Dutch population 从 19 世纪荷兰人的牙结石和骨骼遗骸中探索饮食和疾病模式的多代理分析
Peer community journal Pub Date : 2024-04-23 DOI: 10.24072/pcjournal.414
B. P. Bartholdy, Jørgen B. Hasselstrøm, Lambert K. Sørensen, Maia Casna, Historisch Menno Hoogland, Genootschap Beemster, Amanda G. Henry, Historisch Genootschap Beemster
{"title":"Multiproxy analysis exploring patterns of diet and disease in dental calculus and skeletal remains from a 19th century Dutch population","authors":"B. P. Bartholdy, Jørgen B. Hasselstrøm, Lambert K. Sørensen, Maia Casna, Historisch Menno Hoogland, Genootschap Beemster, Amanda G. Henry, Historisch Genootschap Beemster","doi":"10.24072/pcjournal.414","DOIUrl":"https://doi.org/10.24072/pcjournal.414","url":null,"abstract":"Dental calculus is an excellent source of information on the dietary patterns of past populations, including consumption of plant-based items. The detection of plant-derived residues such as alkaloids and their metabolites in dental calculus provides direct evidence of consumption by individuals within a population. We conducted a study on 41 individuals from Middenbeemster, a 19th century rural Dutch archaeological site. Skeletal and dental analysis was performed to explore potential relationships between pathological lesions and presence of alkaloids. Dental calculus was analysed using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-ESI-MS/MS). We were able to detect nicotine, cotinine, caffeine, theophylline, and salicylic acid, suggesting the consumption of tea and coffee and smoking of tobacco on an individual scale, which is also confirmed by historic documentation and identification of pipe notches in the dentition. Nicotine and/or cotinine was present in 56% of individuals with at least one visible pipe notch. There is some influence of skeletal preservation on the detection of alkaloids, with higher quantities of compounds extracted from well-preserved individuals, and we observe a positive relationship between weight of the calculus sample and quantity of detected compounds, as well as between chronic maxillary sinusitis and the presence of multiple alkaloids. There are many limitations that will need to be addressed going forward with this type of analysis; we stress the need for more systematic research on the consumption of alkaloid-containing items and their subsequent concentration and preservation in dental calculus, in addition to how mode of consumption may affect concentrations in the dentition. Despite the limitations, this preliminary study illustrates many benefits of using calculus to target a variety of compounds that could have been consumed as medicine or diet. This method allows us to directly address specific individuals, which can be especially useful in individuals that are not always well-documented in historic documentation, such as rural populations, and especially children and women.","PeriodicalId":74413,"journal":{"name":"Peer community journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140669171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Transcriptomic responses of sponge holobionts to in situ, seasonal anoxia and hypoxia 海绵全缘体对原位、季节性缺氧和缺氧的转录组反应
Peer community journal Pub Date : 2024-04-15 DOI: 10.24072/pcjournal.410
Brian W. Strehlow, Astrid Schuster, Warren R. Francis, Lisa K Eckford-Soper, Beate Kraft, Rob McAllen, R. Nielsen, S. Mandrup, Donald E. Canfield
{"title":"Transcriptomic responses of sponge holobionts to in situ, seasonal anoxia and hypoxia","authors":"Brian W. Strehlow, Astrid Schuster, Warren R. Francis, Lisa K Eckford-Soper, Beate Kraft, Rob McAllen, R. Nielsen, S. Mandrup, Donald E. Canfield","doi":"10.24072/pcjournal.410","DOIUrl":"https://doi.org/10.24072/pcjournal.410","url":null,"abstract":"Deoxygenation can be fatal for many marine animals; however, some sponge species are tolerant of hypoxia and anoxia. Indeed, two sponge species, Eurypon sp. 2 and Hymeraphia stellif-era, survive seasonal anoxia for months at a time. To understand their tolerance mechanisms, we performed differential gene expression analyses on the sponges, their mitochondria and their microbial symbionts under in situ conditions of normoxia, hypoxia and anoxia. Each species","PeriodicalId":74413,"journal":{"name":"Peer community journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140703084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Social Network Analysis of Ancient Japanese Obsidian Artifacts Reflecting Sampling Bias Reduction 反映取样偏差减少情况的日本古代黑曜石文物社交网络分析
Peer community journal Pub Date : 2024-04-08 DOI: 10.24072/pcjournal.409
Fumihiro Sakahira, Hiro'omi Tsumura
{"title":"Social Network Analysis of Ancient Japanese Obsidian Artifacts Reflecting Sampling Bias Reduction","authors":"Fumihiro Sakahira, Hiro'omi Tsumura","doi":"10.24072/pcjournal.409","DOIUrl":"https://doi.org/10.24072/pcjournal.409","url":null,"abstract":"This study aims to investigate the dynamics of obsidian trade networks during the Jomon period (15,000–2,400 years cal BP), the hunting and gathering era in Japan. To improve regional representation and reduce the distortions caused by small sample sizes, we performed clustering based on a large-scale dataset and conducted social network analysis. The research results revealed that the trade networks during the Jomon period were not constant; they expanded throughout the southern Kanto region during the Middle Jomon period (5,500–4,500 years cal BP) and ceased to function during the Late Jomon period (4,500–3,200 years cal BP). Furthermore, to enhance the readability and inter-pretability of the dataset, we implemented clustering using the density-based spatial clustering of applications with noise (DBSCAN) method. The results showed that in every time division of the Jomon period, the mean intra-cluster cosine similarity of each cluster was higher than the similarity between sites outside the clusters","PeriodicalId":74413,"journal":{"name":"Peer community journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140730473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic background of body reserves in laying hens through backfat thickness phenotyping 通过背膘厚度表型分析蛋鸡体储的遗传背景
Peer community journal Pub Date : 2024-04-04 DOI: 10.24072/pcjournal.412
N. Bedere, Joëlle Dupont, Y. Baumard, Christophe Staub, D. Gourichon, Elleboudt Frédéric, Pascale Le Roy, Tatiana Zerjal
{"title":"Genetic background of body reserves in laying hens through backfat thickness phenotyping","authors":"N. Bedere, Joëlle Dupont, Y. Baumard, Christophe Staub, D. Gourichon, Elleboudt Frédéric, Pascale Le Roy, Tatiana Zerjal","doi":"10.24072/pcjournal.412","DOIUrl":"https://doi.org/10.24072/pcjournal.412","url":null,"abstract":"In this study, we pursued three primary objectives: firstly to test and validate the pheno-typing of backfat thickness as an indicator of the overall fatness of laying hens; secondly, to estimate genetic parameters for this trait; thirdly, to study the phenotypic and genetic relationships between this trait and other traits related to production and body composition. To address these questions, hens from two lines under divergent selection for residual feed intake, were phenotyped for body weight, body composition traits (backfat, total fat volume, and blood adipokines levels), and egg number. Linear mixed models enabled to estimate variance components and calculate","PeriodicalId":74413,"journal":{"name":"Peer community journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140744060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dealing with post-excavation data: the Omeka S TiMMA web-database 处理发掘后数据:Omeka S TiMMA 网络数据库
Peer community journal Pub Date : 2024-01-24 DOI: 10.24072/pcjournal.366
Bastien Rueff
{"title":"Dealing with post-excavation data: the Omeka S TiMMA web-database","authors":"Bastien Rueff","doi":"10.24072/pcjournal.366","DOIUrl":"https://doi.org/10.24072/pcjournal.366","url":null,"abstract":"This paper reports on the creation and use of a web database designed as part of the TiMMA project with the Content Management System Omeka S. Rather than resulting in a technical manual, its goal is to analyze the relevance of using Omeka S in the frame of a post-excavation project, gathering a wide team of researchers from several countries, working in their own language and having their own specialty. Designed to assist organizations and institutions in creating and managing digital collections, Omeka S offers a number of commodities from the perspective of both the administrator(s) and the users, the most significant being its efficiency in capitalizing on linked data standards for items description. Additionally, one of the successful achievements of this platform is to benefit the open-science tools more broadly, permitting for instance, as was done in this project, to import images with the iiiF API and bibliographical references with the Zotero module, thus avoiding the creation of new digital files and metadata. Designed with simplicity in mind, the graphic interface of Omeka S makes it a particularly appropriate tool for","PeriodicalId":74413,"journal":{"name":"Peer community journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139602552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mark loss can strongly bias estimates of demographic rates in multi-state models: a case study with simulated and empirical datasets 标记丢失会对多州模型中的人口统计率估算造成严重偏差:利用模拟数据集和经验数据集进行的案例研究
Peer community journal Pub Date : 2023-12-21 DOI: 10.24072/pcjournal.348
F. Touzalin, Eric J. Petit, Emmanuelle Cam, Claire Stagier, E. Teeling, S. Puechmaille
{"title":"Mark loss can strongly bias estimates of demographic rates in multi-state models: a case study with simulated and empirical datasets","authors":"F. Touzalin, Eric J. Petit, Emmanuelle Cam, Claire Stagier, E. Teeling, S. Puechmaille","doi":"10.24072/pcjournal.348","DOIUrl":"https://doi.org/10.24072/pcjournal.348","url":null,"abstract":"1. The development of methods for individual identification in wild species and the refinement of Capture-Mark-Recapture (CMR) models over the past few decades have greatly improved the assessment of population demographic rates to address ecological and conservation questions. In particular, multi-state models, which offer flexibility in analysing complex study systems, have gained popularity within the ecological community. In this study, we focus on the issue of mark loss and the associated recycling of remarked individuals, which requires further exploration given the increasing use of these models. 2. To fill this knowledge gap, we employed a wide range of simulation scenarios that reflect commonly encountered real case studies, drawing inspiration from the","PeriodicalId":74413,"journal":{"name":"Peer community journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138950442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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