American journal of cancer research最新文献

{"title":"Preoperative red blood cell transfusion improves treatment response and survival in patients with locally advanced esophageal cancer and iron deficiency anemia.","authors":"Jia Dai, Yuzhi Kang, Rong Fu, Li Yan, Yiyu Wang, Lin Li","doi":"10.62347/ZKZM8986","DOIUrl":"10.62347/ZKZM8986","url":null,"abstract":"<p><strong>Background: </strong>Locally advanced esophageal cancer presents significant treatment challenges, with iron deficiency anemia (IDA) potentially hindering the efficacy of neoadjuvant chemotherapy. This study aimed to evaluate the effect of preoperative red blood cell transfusion on tumor progression and chemotherapy response in patients with IDA.</p><p><strong>Methods: </strong>A retrospective cohort study was conducted at Shaanxi Provincial Cancer Hospital, involving 228 patients with locally advanced esophageal cancer and IDA, from August 2015 to August 2021. Patients were divided into two groups: 106 patients received preoperative red blood cell transfusions, and 122 did not. All patients underwent a standardized chemotherapy regimen followed by surgery. Hematological, immunological, and biochemical parameters, as well as chemotherapy-related adverse events, and survival outcomes were analyzed.</p><p><strong>Results: </strong>Preoperative transfusion significantly increased hemoglobin (Hb) levels (P < 0.05), enhanced immunological profiles (e.g., NK/T cell activity; P < 0.05), and reduced systemic inflammation (e.g., CRP; P < 0.05) after chemotherapy and operation. Overall survival and progression-free survival were notably better in the transfusion group (P = 0.002 and P = 0.012, respectively).</p><p><strong>Conclusion: </strong>Preoperative red blood cell transfusion improves hematological and immunological parameters and enhances survival outcomes in patients with locally advanced esophageal cancer and IDA.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 8","pages":"3588-3602"},"PeriodicalIF":2.9,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432581/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LncRNA MIR31HG facilitates immune escape in head and neck squamous cell carcinoma by promoting heterotypic cell-in-cell formation.","authors":"Qigen Fang, Junhui Yuan, Xu Zhang, Lisong Lin","doi":"10.62347/MMZP1913","DOIUrl":"10.62347/MMZP1913","url":null,"abstract":"<p><p>The aggressive nature of head and neck squamous cell carcinoma (HNSCC) is profoundly shaped by a complex interplay between malignant cells and the host immune system. A key feature of this interplay is the formation of cell-in-cell (CIC) structures, which facilitate immune evasion and contribute to shaping the tumor microenvironment. Long non-coding RNAs (lncRNAs), including MIR31HG, have emerged as crucial modulators of tumor immunity and metastasis. In this study, we investigated the role of MIR31HG in orchestrating heterotypic CIC formation and assessed its impact on tumor growth, immune surveillance, and chemoresistance in HNSCC. Analysis of TCGA and GTEx datasets confirmed the differential expression of CIC-related genes between HNSCC and normal tissues. Functional assays demonstrated that CIC structure formation substantially augmented the malignant properties of Cal27 cells, including increased resistance to both cisplatin and gemcitabine. Critically, genetic depletion of MIR31HG in CIC-positive cells (CIC⁺ sh-MIR31HG) potently suppressed tumor progression, as evidenced by reduced cell proliferation and increased apoptosis. Furthermore, silencing MIR31HG also remodeled the immunosuppressive landscape; it downregulated the expression of immune checkpoint protein PD-L1 and decreased the secretion of immunosuppressive cytokines TGF-β and IL-10. This intervention also reversed therapeutic resistance, rendering CIC⁺ sh-MIR31HG cells more susceptible to cisplatin- and gemcitabine-induced apoptosis. These findings were validated in a murine xenograft model, where histological analyses confirmed that tumors originating from CIC⁺ sh-MIR31HG cells displayed reduced volume and elevated apoptotic activity. Collectively, MIR31HG is a pivotal regulator of heterotypic CIC formation in HNSCC. This mechanism promotes HNSCC cell survival, fosters an immunosuppressive microenvironment, and drives chemoresistance. Therefore, targeting MIR31HG represents a viable therapeutic avenue to disrupt immune resistance and enhance chemosensitivity in HNSCC.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 8","pages":"3395-3416"},"PeriodicalIF":2.9,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432567/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors for pulmonary embolism in malignant tumors patients with lower limb deep vein thrombosis: a retrospective study.","authors":"Junfeng Li, Zhen Li, Hui Zheng, Yuan Gao","doi":"10.62347/UPPR8456","DOIUrl":"10.62347/UPPR8456","url":null,"abstract":"<p><strong>Objective: </strong>To identify the risk factors associated with pulmonary embolism (PE) in malignant tumor patients with lower limb deep vein thrombosis (LLDVT).</p><p><strong>Methods: </strong>We retrospectively analyzed the clinical data of 45 patients with PE (observation group) and 255 patients without PE (control group) admitted to The Second Affiliated Hospital of Shandong First Medical University between June 2020 and January 2025. Various clinical parameters, including LLDVT density ratio, D-dimer, homocysteine (Hcy) and cardiac troponin I (cTNI), were compared between the two groups. Logistic regression analysis was performed to identify independent risk factors for PE.</p><p><strong>Results: </strong>The observation group had significantly higher values for LLDVT density ratio (P<0.001), D-dimer (P=0.004), Hcy (P<0.001), cTNI (P<0.001), and Wells scores (P<0.001) compared to the control group. Logistic regression revealed that LLDVT density ratio, Hcy, cTNI, and Wells scores were independent risk factors for PE in these patients. Pearson correlation analysis showed significant positive associations between the LLDVT density ratio (r=0.822, P<0.001), Hcy (r=0.899, P<0.001), cTNI (r=0.890, P<0.001), and Wells scores. ROC curve analysis indicated that the combined model (LLDVT density ratio, Hcy, and cTNI) had a higher AUC (0.852) than individual markers.</p><p><strong>Conclusion: </strong>LLDVT density ratio, Hcy, and cTNI are independent predictors of PE in malignant tumor patients with LLDVT. These markers are closely associated with PE severity, which could assist in optimizing clinical management for these patients.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 8","pages":"3510-3523"},"PeriodicalIF":2.9,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432582/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic validation and clinical translation of CCDC78 as a prognostic biomarker in colorectal cancer.","authors":"Jiang Gong, Binsong Xia, Lei Qian, Yingchang Cai","doi":"10.62347/THYT9980","DOIUrl":"10.62347/THYT9980","url":null,"abstract":"<p><p>This study investigates CCDC78 as a potential prognostic biomarker in colorectal cancer (CRC), incorporating both clinical correlation and functional validation. Analysis of 135 paired tumor and adjacent tissues revealed significantly elevated CCDC78 expression in tumor tissues (P<0.001), and higher expression levels were associated with markedly lower 5-year survival rates (P=0.001). Time-dependent ROC curves demonstrated robust prognostic performance at 12, 36, and 60 months (AUCs of 0.85, 0.84, and 0.82, respectively). In vitro assays showed that CCDC78 overexpression significantly enhanced cell proliferation, migration, and invasion (P<0.05), whereas siRNA-mediated knockdown suppressed these phenotypes and increased apoptosis (P<0.01). Cox regression analyses identified CCDC78 as an independent prognostic factor (P=0.02). Notably, despite similar baseline expression across CRC cell lines, SW480 cells were more sensitive to knockdown, while HCT116 cells more strongly recapitulated the overexpression phenotype. TCGA pan-cancer analysis showed upregulated CCDC78 in various tumors, including CRC, adrenocortical carcinoma (ACC), bladder cancer (BLCA), and kidney renal clear cell carcinoma (KIRC), reinforcing its broad oncogenic relevance. Correlation analyses linked high CCDC78 expression to older age, poor tumor differentiation, advanced TNM stage, lymph node metastasis, and distant metastasis. Immune profiling revealed negative associations with 11 immune cell types but a positive correlation with NK CD56 bright cells. Gene set enrichment analysis (GSEA) implicated CCDC78 in interferon-JAK-STAT, RIG-I/NFκB, and WNT signaling pathways. Altogether, these findings suggest that CCDC78 promotes CRC progression through enhancing tumor cell aggressiveness and modulating the immune microenvironment, underscoring its potential as a prognostic biomarker and therapeutic target.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 7","pages":"3245-3266"},"PeriodicalIF":2.9,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344161/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics profiling reveals PLEKHA6 as a modulator of β-catenin signaling and therapeutic vulnerability in lung adenocarcinoma.","authors":"Bing-Hua Su, Sachin Kumar, Li-Hsin Cheng, Wan-Jung Chang, Dahlak Daniel Solomon, Ching-Chung Ko, Chung-Chieh Chiao, Do Thi Minh Xuan, Juan Lorell Ngadio, Christophorus Manuel Heryanto, Bianca Tobias William, Fitria Sari Wulandari, Hao-Chien Yang, Hung-Yun Lin, Chih-Yang Wang, Ming-Cheng Tsai, Ming-Derg Lai","doi":"10.62347/NVVF8441","DOIUrl":"10.62347/NVVF8441","url":null,"abstract":"<p><p>Lung adenocarcinoma (LUAD) remains the most prevalent and lethal subtype of lung cancer, largely due to late diagnosis and therapeutic resistance. In this study, we conducted a comprehensive multi-omics analysis to characterize the pleckstrin homology domain-containing (PLEKHA) family gene in LUAD. Among the eight members, PLEKHA6 was uniquely overexpressed in LUAD tissues and significantly associated with poor prognosis. Integrated bulk RNA-Seq, single-cell RNA-Seq, DNA methylation, and pharmacogenomic analyses identified PLEKHA6 as a key modulator of oncogenic processes, including Wnt/β-catenin signaling, cadherin-mediated adhesion, and cytoskeletal remodeling. Functional assays in A549 LUAD cells revealed that PLEKHA6 knockdown suppressed β-catenin and VE-cadherin expression, leading to impaired proliferation, migration, and colony formation, along with enhanced apoptosis and cell cycle arrest. Single-cell RNA sequencing demonstrated a correlation between PLEKHA6 expression and tumor-associated macrophage (TAM) infiltration, implicating PLEKHA6 in immune remodeling within the tumor microenvironment (TME). Drug sensitivity analysis and molecular docking further identified potential therapeutic drugs targeting PLEKHA6-expressing LUAD cells. Collectively, our findings establish PLEKHA6 as a novel oncogenic driver and immune modulator in LUAD, supporting its potential as both a prognostic biomarker and a therapeutic target for precision oncology.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 7","pages":"3106-3127"},"PeriodicalIF":2.9,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum: Diffusion MRI biomarkers predict the outcome of irreversible electroporation in a pancreatic tumor mouse model.","authors":"Matteo Figini, Xifu Wang, Tianchu Lyu, Zhanliang Su, Bin Wang, Chong Sun, Junjie Shangguan, Liang Pan, Kang Zhou, Quanhong Ma, Vahid Yaghmai, Daniele Procissi, Andrew C Larson, Zhuoli Zhang","doi":"10.62347/TVVQ2064","DOIUrl":"10.62347/TVVQ2064","url":null,"abstract":"<p><p>[This corrects the article on p. 1615 in vol. 8, PMID: 30210929.].</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 7","pages":"3330-3331"},"PeriodicalIF":2.9,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344178/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical impact and safety of continuous renal replacement therapy in critically ill patients with solid tumors and acute kidney injury: a retrospective cohort analysis.","authors":"Tiangui Luo, Fei Lin, Hengcan Huang","doi":"10.62347/VOTK4097","DOIUrl":"10.62347/VOTK4097","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the efficacy and safety of continuous renal replacement therapy (CRRT) in critically ill patients with solid tumors complicated by acute kidney injury (AKI) in the intensive care unit (ICU).</p><p><strong>Methods: </strong>In this retrospective cohort study, 580 ICU patients with solid tumors and AKI were enrolled and stratified into a CRRT group (n = 300) and a non-CRRT group (n = 280). CRRT was initiated within 12 hours of ICU admission in patients meeting Kidney Disease Improving Global Outcomes stage 3 criteria. Propensity score matching (PSM) was performed to balance baseline characteristics. Primary outcomes included AKI remission, metabolic stabilization, and 90-day survival. Multivariable logistic regression and XGBoost machine learning were used to identify prognostic factors and build predictive models.</p><p><strong>Results: </strong>After PSM, baseline characteristics were well balanced. CRRT significantly improved renal and metabolic parameters within 72 hours, including reductions in serum creatinine, blood urea nitrogen, and lactate (all P < 0.001), with a trend toward increased urine output. The CRRT group had higher AKI remission (64.54% vs. 49.65%, P < 0.001) and 90-day survival rates (47.52% vs. 39.01%, P = 0.012), albeit with greater dialysis dependence (19.15% vs. 0.00%, P < 0.001). Adverse events were comparable. XGBoost models achieved AUCs of 0.78 and 0.75 for mortality and AKI remission, respectively.</p><p><strong>Conclusions: </strong>CRRT improves renal recovery, metabolic status, and survival in critically ill cancer patients with AKI, with an acceptable safety profile. Machine learning offers promising tools for individualized outcome prediction.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 7","pages":"3267-3285"},"PeriodicalIF":2.9,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344166/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural Orifice Specimen Extraction Surgery yields superior long-term oncological outcomes compared to traditional laparoscopic surgery in stage II-III rectal cancer.","authors":"Yanle Fang, Lin Lin, Hongxun Ruan, Xiaoning Qin","doi":"10.62347/UNAS2378","DOIUrl":"10.62347/UNAS2378","url":null,"abstract":"<p><strong>Objectives: </strong>To compare the long-term oncological and clinical outcomes of natural orifice specimen extraction surgery (NOSES) versus traditional laparoscopic surgery (TLS) in patients with Stage II-III rectal cancer.</p><p><strong>Methods: </strong>This retrospective cohort study analyzed data from 320 patients who underwent curative resection for Stage II-III rectal cancer between January 2020 and January 2025. Of these, 162 received NOSES and 158 underwent TLS. Perioperative outcomes, postoperative recovery, complications, disease-free survival (DFS), overall survival (OS), recurrence and metastasis rates, and quality of life were evaluated. Multivariate analyses were performed to identify independent prognostic factors for long-term outcomes.</p><p><strong>Results: </strong>Compared with TLS, NOSES resulted in shorter operative times and less intraoperative blood loss (both P < 0.001). Patients in the NOSES group experienced faster return of bowel function and shorter hospital stays (both P < 0.001). At five-year follow-up, NOSES was associated with significantly improved DFS (P = 0.014), OS (P = 0.009), and higher quality of life scores (P < 0.001). The NOSES group also exhibited fewer postoperative complications and a lower incidence of distant metastasis (P = 0.034). Multivariate analysis identified NOSES as an independent predictor of improved long-term survival and quality of life.</p><p><strong>Conclusions: </strong>NOSES offers significant advantages over TLS in the management of Stage II-III rectal cancer, including enhanced operative efficiency, accelerated recovery, reduced complications, and superior long-term oncological outcomes and quality of life. These findings support the wider clinical adoption of NOSES as a preferred minimally invasive surgical approach in eligible rectal cancer patients.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 7","pages":"3286-3298"},"PeriodicalIF":2.9,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on sedentary behavior and its influencing factors in elderly ovarian cancer patients during home confinement.","authors":"Saie Zhu, Limeng Cai, Shanshan Chen, Dandan Zhao, Yi Chen, Yulin Zhou, Ning Zhou, Jiaojiao Jin","doi":"10.62347/ZTPJ8215","DOIUrl":"10.62347/ZTPJ8215","url":null,"abstract":"<p><p>Sedentary behavior is prevalent among the elderly and has become an increasingly serious public health issue. Although extensive research has been conducted on the status and risk factors of sedentary behavior in the general elderly population, the current situation and related influencing factors of sedentary behavior in elderly patients with ovarian cancer, a disease-specific group, have not been fully explored. In this study, a total of 206 elderly ovarian cancer (EOC) patients who received treatment at the First Affiliated Hospital of Zhejiang University School of Medicine from September 1, 2022, to February 28, 2025, were selected as the research subjects by convenience sampling. A cross-sectional survey was conducted using the General Information Questionnaire, Chinese Adult Sedentary Behavior Questionnaire, Social Support Rating Scale, Nutritional Risk Screening 2002, and Edmonton Symptom Assessment Scale. The influencing factors of sedentary behavior in EOC patients were analyzed by the logistic regression model and CHAID decision tree model. Among the 206 EOC patients, the average sedentary time was 7.4±3.0 h/d, and 161 patients (78.2%) had sedentary behavior (sedentary time ≥5 h/d). Logistic regression analysis and CHAID decision tree algorithm both demonstrated that social support and cancer symptom burden were the influencing factors of sedentary behavior in EOC patients (P<0.05). Moreover, the Chi-square Automatic Interaction Detection (CHAID) algorithm further revealed an interaction between the two factors, indicating that the social support level was the most crucial determinant. Our study reveals that sedentary behavior among EOC patients is alarmingly prevalent, necessitating urgent attention from medical professionals. Given the significant impact of social support and cancer symptom burden on this sedentary behavior, healthcare providers should proactively assess and intervene to address these influential factors, thereby mitigating the adverse consequences of excessive sedentary time for EOC patients. Decision tree and logistic regression models effectively identify sedentary behavior determinants with good predictive power. A combined approach is recommended to leverage their complementary strengths, providing a robust basis for reducing sedentary behavior in EOC patients.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 7","pages":"3310-3322"},"PeriodicalIF":2.9,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Peptostreptococcus anaerobius</i> promotes cervical cancer angiogenesis by upregulating SCD to activate ERK pathway.","authors":"Yingxin Gong, Guannan Zhou, Yuanyuan Gu, Menglei Zhang, Ganrong Zhang, Zheng Gu, Junhao Chen, Hang Zhou, Jingxin Ding","doi":"10.62347/DYUN7645","DOIUrl":"10.62347/DYUN7645","url":null,"abstract":"<p><p>Accumulating evidence indicates that virginal microbiota dysbiosis is a distinct feature of cervical cancer. As cervical lesions progress towards malignancy, the dominance of <i>Lactobacillus</i> species within the vaginal microbiota is progressively replaced by anaerobic bacteria, with <i>Peptostreptococcus anaerobius</i> (<i>P. anaerobius</i>) being a noticeable one. Despite this well-documented microbial shift, the precise functional role of <i>P. anaerobius</i> in cervical cancer development and progression has remained unclear. Our study demonstrated that <i>P. anaerobius</i> promoted cervical cancer cells proliferation and enhanced tube formation of human umbilical vein endothelial cells (HUVECs). Furthermore, we identified a significant upregulation of stearoyl-CoA desaturase 1 (SCD) following the introduction of <i>P. anaerobius</i>, leading to subsequent activation of the extracellular signal-regulated kinase (ERK) signaling pathway. Moreover, supplement with <i>P. anaerobius</i> failed to reverse the ERK1/2 inhibitor-induced suppression of the tube-formation. <i>In vivo</i> validation revealed that <i>P. anaerobius</i> exerted its influence on angiogenesis by regulating SCD expression and ERK pathway acvivation. Collectively, these findings reveal an oncogenic role of <i>P. anaerobius</i> in cervical cancer, mediated by the SCD-ERK signaling axis to drive angiogenesis. This work provides novel mechanistic insights into the contribution of vaginal microbiota to gynecologic malignancies.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 7","pages":"3236-3244"},"PeriodicalIF":2.9,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}