American journal of cancer research最新文献

{"title":"Erratum: Baicalein suppress EMT of breast cancer by mediating tumor-associated macrophages polarization.","authors":"Xixi Zhao, Jingkun Qu, Xu Liu, Jizhao Wang, Xingcong Ma, Xiaoyao Zhao, Qian Yang, Wanjun Yan, Zitong Zhao, Yuxin Hui, Haocheng Bai, Shuqun Zhang","doi":"10.62347/RFQT4858","DOIUrl":"https://doi.org/10.62347/RFQT4858","url":null,"abstract":"<p><p>[This corrects the article on p. 1528 in vol. 8, PMID: 30210921.].</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 6","pages":"2905-2907"},"PeriodicalIF":3.6,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12256409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors for postoperative vascular crisis in squamous cell carcinoma reconstruction using supraclavicular artery flaps.","authors":"Peipei Sun, Mingzhe Zhao, Hanying Tang","doi":"10.62347/ZHMS1734","DOIUrl":"10.62347/ZHMS1734","url":null,"abstract":"<p><strong>Objectives: </strong>To identify risk factors associated with vascular crisis in patients undergoing reconstruction with supraclavicular artery flaps for squamous cell carcinoma (SCC) of the tongue and buccal mucosa.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on 777 patients with tongue or buccal SCC who underwent supraclavicular artery flap reconstruction between January 2019 and December 2023. Patients were divided into two groups based on the occurrence of postoperative vascular crisis: Occurred Group (n = 101) and No Occurred Group (n = 676). Demographic data, clinical history, hematologic and biochemical parameters were collected. Pearson and Spearman correlation analyses, univariate analysis, and multivariate logistic regression were performed to identify independent risk factors. An external validation cohort was used to verify the findings, and a predictive model was developed using ROC curve and nomogram analysis.</p><p><strong>Results: </strong>Independent risk factors for vascular crisis included higher BMI, long-term smoking, long-term alcohol consumption, elevated fasting blood glucose, increased C-reactive protein, higher white blood cell count, and elevated SCC antigen (all P < 0.05). Platelet count was inversely associated with risk. Flap survival rate was significantly lower in the vascular crisis group. The predictive model demonstrated strong discriminatory power (AUC = 0.975).</p><p><strong>Conclusions: </strong>Several modifiable clinical and biochemical factors are significantly associated with postoperative vascular crisis. Preoperative optimization of these variables may improve flap survival and surgical outcomes.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 6","pages":"2855-2871"},"PeriodicalIF":3.6,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12256421/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic and prognostic value of coagulation markers and platelet-derived growth factor-BB in evaluating intensity-modulated radiotherapy efficacy in nasopharyngeal carcinoma.","authors":"Haizhong Zhang, Deli Ye","doi":"10.62347/MQBC5709","DOIUrl":"10.62347/MQBC5709","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the diagnostic and prognostic value of coagulation markers - including activated partial thromboplastin time (APTT), prothrombin time (PT), fibrinogen (FIB), platelet count (PLT), and D-dimer (DD) - and platelet-derived growth factor-BB (PDGF-BB) in patients with nasopharyngeal carcinoma (NPC) undergoing intensity-modulated radiotherapy (IMRT).</p><p><strong>Methods: </strong>A total of 210 NPC patients receiving IMRT and 160 healthy controls were enrolled. Baseline levels of PDGF-BB and coagulation markers were compared between groups. The association of PDGF-BB with clinical staging was analyzed, and receiver operating characteristic (ROC) curve analysis was used to assess its diagnostic performance. Cox regression analyses were performed to identify independent predictors of five-year survival. A dynamic nomogram was developed to provide individualized survival predictions.</p><p><strong>Results: </strong>NPC patients exhibited significantly higher levels of PDGF-BB, APTT, PT, FIB, PLT, and DD compared to healthy controls (all P < 0.001). PDGF-BB was positively correlated with TNM stage (stage III/IV vs. I/II, P < 0.001), T stage (P = 0.005), and N stage (P = 0.020). Multivariate Cox regression identified low PDGF-BB (< 628.18) (HR = 0.492, P = 0.009), low DD (< 746.1) (HR = 0.456, P = 0.002), age 51-64 years (HR = 2.057, P = 0.032) and ≥ 65 years (HR = 4.138, P < 0.001), EBV DNA negativity (HR = 0.273, P = 0.012), and TNM stage III/IV (HR = 3.042, P = 0.023) as independent prognostic factors.</p><p><strong>Conclusions: </strong>PDGF-BB and DD, alongside age, EBV DNA status, and TNM stage, are promising biomarkers for NPC prognosis. A dynamic nomogram integrating these factors offers accurate survival prediction and supports personalized treatment strategies in NPC management.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 6","pages":"2451-2468"},"PeriodicalIF":3.6,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12256396/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early detection of biliary adenocarcinoma using probe-based confocal laser endomicroscopy: a case report.","authors":"Jiangshan Wu, Jingnan Lin, Chenglong Fan, Chengli Liu","doi":"10.62347/LEUO1423","DOIUrl":"10.62347/LEUO1423","url":null,"abstract":"<p><strong>Background: </strong>Adenocarcinoma is the most common malignant tumor of the bile duct, originating from the mucosal epithelium. Surgical resection is typically recommended for biliary adenocarcinoma to achieve the best therapeutic outcome and prognosis. However, early detection and diagnosis remain significant global challenges.</p><p><strong>Case presentation: </strong>This paper presents a case of a 55-year-old female patient who was admitted to the Second People's Hospital of Nanning with complaints of dull pain in the right upper abdomen for 4 months and yellowing of the skin and sclera for the past half month. The patient was diagnosed as early well-differentiated biliary adenocarcinoma by probe-based confocal laser endomicroscopy (pCLE), biliary ultrasonography, choledochoscopy and biopsy. After pancreaticoduodenectomy, exploratory laparotomy, partial bowel resection, afferent loop and efferent loop anastomosis, the patient underwent surgery successfully. After 1 year of surgical treatment, no significant postoperative complications were found in the follow-up. There was no recurrence after operation and the patient made a full recovery.</p><p><strong>Conclusion: </strong>Bile duct adenocarcinoma is a malignant tumor, and early screening, diagnosis and treatment are crucial for improving patient prognosis. In this case, early cholangiocarcinoma was detected by a combination of choledochoscopy, biliary ultrasound, and biliary confocal endoscopy. The tumor and surrounding tissues were completely removed through pancreaticoduodenectomy. The operation was successful and the patient recovered well. Confocal microendoscopy shows great promise in the early diagnosis of digestive tract diseases, owing to its high-resolution imaging capabilities and potential to detect early lesions.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 6","pages":"2642-2649"},"PeriodicalIF":3.6,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12256425/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thyroid hormone suppresses cell growth by regulating CDK2 and cyclin E1 expression via Hepsin.","authors":"Yang-Hsiang Lin, Meng-Han Wu, Chia-Jung Liao, Yu-Tung Lin, Chau-Ting Yeh, Kwang-Huei Lin","doi":"10.62347/AKVV2832","DOIUrl":"10.62347/AKVV2832","url":null,"abstract":"<p><p>Thyroid hormone (T3) and its receptor (TR) play crucial roles in regulating cell proliferation and cancer progression, including hepatocellular carcinoma (HCC). However, the specific mechanisms underlying HCC development mediated by T3/TR remain unclear. This study aimed to identify differentially expressed target genes influenced by T3/TR in HCC progression. Microarray profiling analysis revealed hepsin (HPN) as a potential target gene regulated by T3/TR. Quantitative reverse transcription-PCR (qRT-PCR) confirmed that T3/TR upregulates HPN expression. Promoter assays and chromatin immunoprecipitation (ChIP) analysis further demonstrated that TR directly binds to the HPN promoter region (+506/+523), activating its transcription. Functional studies showed that ectopic expression of HPN significantly inhibited cell proliferation. Furthermore, HPN was found to be involved in T3/TR-mediated suppression of cell growth by modulating the expression of CDK2 and cyclin E1. Clinically, HPN expression levels were inversely correlated with CDK2 and cyclin E1 in HCC tissues. These findings establish a novel regulatory relationship among T3/TR, HPN, CDK2, and cyclin E1, highlighting their potential role in controlling liver cancer cell proliferation.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 6","pages":"2595-2603"},"PeriodicalIF":3.6,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12256398/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic profiling of PBMCs from mammary tumor dogs reveals two distinct immune states.","authors":"Kang-Hoon Lee, Dabin Lee, Jeong-Woon Lee, Hyeon-Ji Hwang, Je-Yoel Cho","doi":"10.62347/LOJB9067","DOIUrl":"10.62347/LOJB9067","url":null,"abstract":"<p><p>This study analyzed cancer-specific systemic immune responses in peripheral blood mononuclear cells (PBMCs) from dogs with benign tumors, malignant tumors, and normal conditions. By examining gene expression patterns - particularly immune checkpoint and TNFRSF genes - the study aimed to assess the immune state of cancer PBMCs. Surprisingly, half of the tumor PBMCs exhibited downregulation of both immunosuppressive genes (Pdcd1, Ctla4, Tigit) and immune activation molecules (CD27, CD357), suggesting immune inactivity rather than suppression. Additionally, cytokine expression varied significantly, with upregulation of IL-18 and IL-7, despite their controversial roles in tumor progression. Analysis of T-cell exhaustion markers did not reflect established exhaustion signatures, implying a naive-like immune state. Instead, a distinct immune signature emerged, characterized by the broad downregulation of TNFRSF genes (TNFRSF18, TNFRSF14, TNFRSF6, and CD27). We designated this group as PI (PBMC-impaired). Deconvolution of bulk RNA-seq data further revealed a significant reduction in CD4+ T cells and a lower CD4+/CD8+ ratio in the PI group. Gene Ontology (GO) and pathway analyses linked CD4+ cell differentially expressed genes (DEGs) to regulatory T-cell differentiation, inflammatory responses, and key immune pathways (IL-2/STAT5, NF-kappa B). Notably, CD7, CXCL6, FASN, FLT3LG, LTB, and TNFRSF18 were significantly downregulated, marking a potential transcriptomic signature of systemic immune impairment. These findings suggest that immune dysfunction in the PI group is not solely attributable to conventional immune suppression but rather to a diminished immune activation state driven by reduced TNFRSF gene expression.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 6","pages":"2564-2578"},"PeriodicalIF":3.6,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12256404/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Imaging-guided SMART improves survival in locally advanced and borderline resectable pancreatic cancer: a comparative study.","authors":"Nianhui Jiao, Huiqin Qi, Xuejun Li, Yongjie Qi, Yanjie Sun","doi":"10.62347/XKSS2851","DOIUrl":"10.62347/XKSS2851","url":null,"abstract":"<p><p>Pancreatic cancer remains notoriously challenging to treat due to its aggressive nature and complex anatomic location. Late-stage diagnoses often result in high mortality rates. This study assesses the effectiveness of combining ablative stereotactic MRI-guided intensity-modulated radiation therapy (SMART) with chemotherapy for treating locally advanced and borderline resectable pancreatic cancer. We retrospectively analyzed 235 pancreatic cancer patients treated between 2020 and 2023. Patients were divided into chemoradiation (SMART + chemotherapy, n = 106) and chemotherapy-only (n = 129) groups. Key outcomes included progression-free survival, overall survival, margin-negative resection rates, lymphovascular invasion, and toxicities. The chemoradiation group demonstrated improved PFS (8.30 ± 1.20 vs. 7.90 ± 1.30 months, P = 0.015) and OS (14.30 ± 2.60 vs. 13.50 ± 2.40 months, P = 0.015), with higher rates of margin-negative resections (92.45% vs. 80.62%, P = 0.009) and reduced LVI (37.74% vs. 52.71%, P = 0.022) compared to chemotherapy alone. However, acute toxicities, including fatigue and abdominal pain, were more frequent in the chemoradiation group. Locoregional control and distant metastasis-free survival showed no significant group differences (P > 0.05). Overall, SMART enhances local tumor control and survival outcomes in severe pancreatic cancer, albeit with increased acute toxicity.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 6","pages":"2604-2617"},"PeriodicalIF":3.6,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12256400/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The therapeutic potential of repurposed mebendazole, alone and in synergistic combination with ONC201, in the treatment of diffuse midline glioma.","authors":"Serena Gentile, Federica Toma, Donatella Lucchetti, Lucia Lisi, Pierluigi Navarra, Alessandro Sgambato, Tiziana Servidei, Antonio Ruggiero","doi":"10.62347/MXZH5646","DOIUrl":"10.62347/MXZH5646","url":null,"abstract":"<p><p>H3K27-altered diffuse midline glioma (DMG) is a universally fatal disease with no available therapeutic strategies apart from palliative radiotherapy. Repurposing marketed non-cancer drugs in oncology is emerging as a fast-tracking approach to speed up the development of new treatment options, urgently needed for DMG. Repurposed anthelmintic mebendazole (MBZ) is in the spotlight against brain tumors, because it joins promising anticancer properties with high neuropenetrance, favorable pharmacokinetic and safety profile. Although MBZ is undergoing Phase I/II trials against brain tumors, including DMG, MBZ anticancer properties and the underlying mechanisms of actions have poorly been characterized in DMG preclinical models. We found that MBZ robustly reduced cell viability in six out of seven DMG cell lines with either K27M-mutated or wild-type H3. All IC₅₀ values (range 102 to 958 nM) fell in a clinically attainable range. The antiproliferative MBZ properties were mediated by an arrest of DMG cells in the G₂/M phase with a concomitant upregulation of the key cell cycle regulators p21 and p27, whereas p53 upregulation and activation were cell context-dependent. At the same growth-inhibitory concentrations, MBZ triggered apoptotic cell death, as evidenced by higher levels of the apoptotic markers caspase-3 and PARP cleavage. Consistently, Annexin V-Propidium iodide (PI) double staining showed MBZ dose-dependent increase in both stages of apoptosis. Of interest, the combination of MBZ with the first-in-class imipridone ONC201 sinergistically increased the antiproliferative effects in two DMG cell lines as assessed by combination scores with different algorithms, showing additive effects in two others cell lines. Mechanistically, the combination potentiated the proapoptotic activity of either MBZ or ONC201, while not changing the cytokinetic perturbations induced by the single drugs. Finally, one pair of ONC201-sensitive and ONC201-resistant DMG cell lines with acquired resistance showed same responsiveness to MBZ with similar values of IC₅₀ and E_max. In conclusion, MBZ demonstrates high growth-inhibitory/proapoptotic activity, chemosensitization property to ONC201 and the ability to overcome ONC201 resistance in DMG cell cultures, proposing as a new low-toxicity therapeutic for DMG, with a potential to be used in second-line treatment and/or in combination protocols.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 6","pages":"2701-2718"},"PeriodicalIF":3.6,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12256405/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intraosseous versus intravenous fluid resuscitation in gastrointestinal tumor-related acute hemorrhage: impact on 30-day mortality and lactate clearance.","authors":"Juan Chen, Chunmei Zhang, Xiaowei He, Xiaoqin Han, Yingzhe Su, Chunyan Chen, Wenxia Xu, Wei Yang","doi":"10.62347/EAMR9595","DOIUrl":"10.62347/EAMR9595","url":null,"abstract":"<p><p>This retrospective study evaluated the impact of intraosseous infusion (IO) versus traditional intravenous infusion (IV) on 30-day mortality and clinical outcomes in 518 patients with acute gastrointestinal bleeding (AGIB) secondary to gastrointestinal tumors from January 2022 to July 2024. Patients were divided into IO (n=217) and IV (n=301) groups based on initial resuscitation strategy. Compared to IV group, the IO group demonstrated higher first-attempt catheterization success rate, shorter vascular access time, and faster blood pressure recovery (all P<0.001), alongside higher 6-hour lactate (LA) clearance (34% vs. 22%, P<0.001) and lower 30-day mortality (11.98% vs. 18.6%, P=0.016). Multivariate analysis identified IO infusion as protective factor for lactate metabolism (HR=0.289, 95% CI: 0.092-0.864), while advanced age (HR=1.125), diabetes (HR=3.23), and low LA clearance (HR=0.016) were independent risk factor for mortality. Causal mediation analysis revealed that 6-hour LA clearance mediated 68% of the IO-associated mortality reduction (P<0.001), whereas diabetes history was not a significant mediator (P=0.156). Complication rates were comparable between groups (P>0.05). These findings indicate that IO infusion improves survival in AGIB due to gastrointestinal tumors by rapidly restoring hemodynamics and enhancing lactate metabolism. The mortality benefit is primarily driven by accelerated LA clearance rather than comorbidities like diabetes. Given its safety profile comparable to IV, IO infusion should be prioritized in critical care settings.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 6","pages":"2682-2700"},"PeriodicalIF":3.6,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12256426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PINX1 inhibits proliferation and cisplatin resistance in nasopharyngeal carcinoma by promoting ILF3 ubiquitination.","authors":"Ni Zhou, Pinggui Gong, Shuilian Wang, Cui He, Liya Hu, Hong Peng","doi":"10.62347/WFER2605","DOIUrl":"10.62347/WFER2605","url":null,"abstract":"<p><p>PIN2/TRF1-interacting telomerase inhibitor 1 (PINX1) acts as a tumor suppressor in various cancers, yet its molecular role in nasopharyngeal carcinoma (NPC) remains poorly defined. This study investigates the therapeutic potential of PINX1 in NPC. Expression levels of PINX1 and interleukin enhancer-binding factor 3 (ILF3) were assessed in NPC cells and tissues via western blotting and immunohistochemistry, and their correlation with patient prognosis was analyzed. The effects of ILF3 on NPC cell proliferation and cisplatin resistance were evaluated using cell cycle analysis, EdU incorporation, CCK-8, and IC₅₀ assays. Co-immunoprecipitation and immunofluorescence confirmed the interaction between PINX1 and ILF3, while qPCR and western blotting assessed their regulatory relationship. Bioinformatics analysis, chromatin immunoprecipitation, and dual-luciferase assays were performed to identify transcription factors regulating PINX1. Additional in vitro experiments explored the antagonistic relationship between PINX1 and ILF3. Results showed that PINX1 expression was downregulated in NPC and associated with favorable prognosis, whereas ILF3 was upregulated and linked to poor outcomes. PINX1 physically interacted with ILF3 and promoted its ubiquitination through Speckle-type BTB/POZ protein (SPOP). Furthermore, signal transducer and activator of transcription 3 suppressed PINX1 transcription, while PINX1 antagonized the oncogenic effects of ILF3. Mechanistically, PINX1 facilitated ILF3 degradation via SPOP, suppressed the PI3K-AKT-mTOR pathway, inhibited tumor proliferation, and enhanced cisplatin sensitivity in NPC cells. These findings highlight the tumor-suppressive role of PINX1 and underscore its potential as a therapeutic target in NPC.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 6","pages":"2518-2534"},"PeriodicalIF":3.6,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12256408/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}