One health advancesPub Date : 2023-01-01Epub Date: 2023-04-30DOI: 10.1186/s44280-023-00012-0
Mengqi Yuan, Yanzhi Zhu, Guanlan Liu, Yujie Wang, Guanxi Wang, Guozhong Zhang, Lilin Ye, Zhaohui Qian, Pinghuang Liu
{"title":"An RBD bispecific antibody effectively neutralizes a SARS-CoV-2 Omicron variant.","authors":"Mengqi Yuan, Yanzhi Zhu, Guanlan Liu, Yujie Wang, Guanxi Wang, Guozhong Zhang, Lilin Ye, Zhaohui Qian, Pinghuang Liu","doi":"10.1186/s44280-023-00012-0","DOIUrl":"10.1186/s44280-023-00012-0","url":null,"abstract":"<p><p>Potent neutralizing antibodies (nAbs) against SARS-CoV-2 are a promising therapeutic against the ongoing COVID-19 pandemic. However, the continuous emergence of neutralizing antibody escape variants makes it challenging for antibody therapeutics based on monospecific nAbs. Here, we generated an IgG-like bispecific antibody (bsAb), Bi-Nab, based on a pair of human neutralizing antibodies targeting multiple and invariant sites of the spike receptor binding domain (RBD): 35B5 and 32C7. We demonstrated that Bi-Nab exhibited higher binding affinity to the Delta spike protein than its parental antibodies and presented an extended inhibition breadth of preventing RBD binding to angiotensin-converting enzyme 2 (ACE2), the cellular receptor of SARS-CoV-2. In addition, pseudovirus neutralization results showed that Bi-Nab improved the neutralization potency and breadth with a lower half maximum inhibitory concentration (IC<sub>50</sub>) against wild-type SARS-CoV-2, variants being monitored (VBMs) and variants of concern (VOCs). Notably, the IgG-like Bi-Nab enhanced the neutralizing activity against Omicron variants with potent capabilities for transmission and immune evasion in comparison with its parental monoclonal antibody (mAb) 32C7 and a cocktail (with the lowest IC<sub>50</sub> values of 31.6 ng/mL against the Omicron BA.1 and 399.2 ng/mL against the Omicron BA.2), showing evidence of synergistic neutralization potency of Bi-Nab against the Omicron variants. Thus, Bi-Nab represents a feasible and effective strategy against SARS-CoV-2 variants of concern.</p>","PeriodicalId":74344,"journal":{"name":"One health advances","volume":"1 1","pages":"12"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10173222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9901491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
One health advancesPub Date : 2023-01-01Epub Date: 2023-03-29DOI: 10.1186/s44280-023-00011-1
Jianzhong Shen, Stefan Schwarz
{"title":"Introducing One Health Advances: a new journal connecting the dots for global health.","authors":"Jianzhong Shen, Stefan Schwarz","doi":"10.1186/s44280-023-00011-1","DOIUrl":"10.1186/s44280-023-00011-1","url":null,"abstract":"","PeriodicalId":74344,"journal":{"name":"One health advances","volume":"1 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10049891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9898021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}