One health advances最新文献

{"title":"Resveratrol mitigates triclosan-induced pyroptosis in chicken spleen by improving immune imbalance and alleviating oxidative stress","authors":"Haoran Zhang, Chunye He, Shuoyan Cui, Rui Chen, Shengzi Jin, Runxiang Zhang","doi":"10.1186/s44280-026-00112-7","DOIUrl":"https://doi.org/10.1186/s44280-026-00112-7","url":null,"abstract":"Abstract Triclosan (TCS) is a broad-spectrum antibacterial agent widely incorporated into daily chemical products. It enters the environment primarily through domestic wastewater, posing risks to animals, plants, and human health. Resveratrol (RES) is a plant-derived phenolic antioxidant with well-documented antioxidant and anti-inflammatory properties. This study aimed to elucidate the molecular mechanisms underlying TCS-induced spleen injury and the protective effects of RES. In this study, sixty 10-week-old Hy-Line Pink chickens were randomly divided into three groups: control group (corn oil), TCS exposure group (150 mg/kg/day body weight, oral gavage), and TCS + RES alleviation group (TCS at 150 mg/kg/day body weight + RES at 400 mg/kg in diet) for four weeks. The impacts of TCS on the chicken spleen as well as the ameliorative effects of RES were investigated using network toxicology, hematoxylin and eosin staining, immunofluorescence, biochemical assays, and gene expression analyses. The results demonstrated that RES effectively alleviated TCS-induced structural abnormalities in splenic tissue, including blurred red-white pulp boundaries, reduced white pulp, red pulp congestion, and expanded interstitial spaces. The protective effects of RES were potentially associated with the expression of macrophage migration inhibitory factor, aryl hydrocarbon receptor, and ATP-binding cassette transporter G2. Furthermore, RES mitigated TCS-induced immune imbalance—including dysregulation of M1/M2 polarization, type 1 helper T cell/type 2 helper T cell imbalance, and cluster of differentiation 4 positive T cells infiltration—as well as oxidative stress and pyroptosis. This study provides novel insights into the toxicological mechanisms of TCS on the spleen, underscores the therapeutic potential of RES against bactericide-induced splenic toxicity, and offers a theoretical foundation for the safer use of antibacterial agents in daily life and poultry production.","PeriodicalId":74344,"journal":{"name":"One health advances","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Animals as overlooked victims and vectors of tuberculosis: review of <i>Mycobacterium tuberculosis</i> and <i>Mycobacterium bovis</i>.","authors":"Rachel C Sadoff, Kunchok Dorjee","doi":"10.1186/s44280-026-00136-z","DOIUrl":"10.1186/s44280-026-00136-z","url":null,"abstract":"<p><p>Tuberculosis (TB) is the world's leading infectious disease in humans usually caused by <i>Mycobacterium tuberculosis</i> (Mtb). However, <i>Mycobacterium bovis</i> can also lead to TB in humans and is the most common TB-causing bacteria in animals. Roughly 140,000 people fall sick from <i>M. bovis</i> every year, and tens of millions of animals are estimated to have TB infection, but there are major barriers to diagnosis and treatment in both humans and animals. For example, most standard TB tests do not detect <i>M. bovis</i>. <i>M. bovis</i> is also naturally resistant to pyrazinamide, a first-line TB drug, and livestock, wildlife, and zoo animals can function as TB reservoirs, infecting new people in otherwise TB-free settings. Effective control measures and integrated strategies targeting Mtb, <i>M. bovis</i>, and other TB-causing bacteria across human, animal, and environmental domains are essential to significantly reduce transmission and achieve global objectives such as the World Health Organization's END TB Strategy. This review therefore takes a \"One Health\" approach, which focuses on three pillars and the connections between them: human health, animal health, and the health of their shared environment. One Health is a helpful framework for understanding complex threats like TB because it reflects the importance and interconnectedness of all three priorities, and can inspire solutions that are adaptive, interdisciplinary, and sustainable.</p>","PeriodicalId":74344,"journal":{"name":"One health advances","volume":"4 1","pages":"25"},"PeriodicalIF":5.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13350205/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148427188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Size-dependent tuning of magnetic metal‒organic frameworks enables high-performance immunochromatographic assays","authors":"C. Y. Zhang, Yanting Li, Yanru Wang, Fei Yang, Xing Shen, Zhenlin Xu, Hongtao Lei, Xiangmei Li","doi":"10.1186/s44280-025-00104-z","DOIUrl":"https://doi.org/10.1186/s44280-025-00104-z","url":null,"abstract":"Abstract To investigate the effect of carrier particle size on immunoassay detection performance, three magnetic metal‒organic frameworks (Fe₃O₄@MOF-545) with different particle sizes (300 nm, 400 nm, and 500 nm) were designed and prepared as antibody carriers, which were successfully conjugated with salbutamol antibodies to construct a series of detection probes. On this basis, a highly sensitive immunochromatographic assay (ICA) platform was established, and the influence of carrier particle size on detection performance was systematically explored. The results revealed that as the particle size of Fe₃O₄@MOF-545 increased, the probe affinity constant significantly increased (5.23 × 10⁷ to 7.39 × 10⁷), and the dissociation constant effectively decreased (1.27 × 10⁻⁸ to 0.83 × 10⁻⁸), indicating a significant increase in the antigen‒antibody binding capacity; the limit of detection was greatly reduced (0.33 μg/kg to 0.15 μg/kg), with sensitivity improved by more than 50%; the equilibrium time of immune kinetic reactions was shortened by 2 min, significantly accelerating the detection speed. These key data clearly reveal that carrier particle size is a core factor regulating ICA analytical performance. This work not only provides a new perspective on the interaction mechanism between nanocarriers and antibodies but also lays an important foundation for the design of next-generation high-performance immunoassay platforms with broad application prospects.","PeriodicalId":74344,"journal":{"name":"One health advances","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147914250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xanthohumol restores the susceptibility of Escherichia coli to colistin","authors":"Ning Han, Feifei Zhao, Yangyang Li, Wenguang Xiong, Peng Wan, Zhenling Zeng","doi":"10.1186/s44280-025-00096-w","DOIUrl":"https://doi.org/10.1186/s44280-025-00096-w","url":null,"abstract":"Abstract The spread of colistin (CL) resistance poses a considerable threat to antibiotic stewardship. Antibiotic adjuvants in combination with antibiotics have become one of the strategies used to mitigate antibiotic resistance. We analyzed the synergistic effect of xanthohumol (XN) with CL via checkerboard tests, temporal bactericidal curve assays and resistance evolution. The synergistic mechanism was explored by testing the outer and inner cell membranes and physiological pathways. The results showed that XN had synergistic activity with CL against Gram-negative bacteria such as mucin-resistant Escherichia coli and delayed the development of CL resistance. Mechanistic studies revealed that XN enhanced the destructive effects of CL on the outer and inner membranes, inhibited intracellular adenosine triphosphate synthesis, and promoted the accumulation of reactive oxygen species. In in vivo studies, the combination of XN and CL also enhanced the therapeutic effect. These results suggest that XN is a potential adjuvant of CL against CL-resistant pathogens.","PeriodicalId":74344,"journal":{"name":"One health advances","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s44280-025-00096-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of fosS, a novel fosfomycin-modifying enzyme gene identified in Staphylococcus haemolyticus","authors":"Honghao Huang, Huan Chen, Ruonan Zhao, Yiyi Chen, Jianxin Hu, Peng Wan, Lingxuan Zhang, Wengaung Xiong, Yan Chen, Yafei Li, Zhenling Zeng","doi":"10.1186/s44280-025-00095-x","DOIUrl":"https://doi.org/10.1186/s44280-025-00095-x","url":null,"abstract":"Abstract The efficacy of fosfomycin is increasingly challenged by emerging resistance mechanisms, primarily involving fosfomycin-modifying enzymes and target (MurA) alterations. This study aimed to elucidate the novel mechanism underlying fosfomycin resistance in fosfomycin-resistant Staphylococcus haemolyticus (FRSH) strains. Whole-genome sequencing and bioinformatic analysis identified a novel fosfomycin-modifying enzyme, designated FosS , and new mutations (D290H/G359S) on MurA. Cloning experiments further demonstrated that the expression of fosS increased the minimum inhibitory concentration of fosfomycin 32-fold in S. aureus RN4220, whereas MurA mutations induced a 4-fold increase in minimum inhibitory concentration. Phylogenomic analysis revealed that fosS -positive S. haemolyticus (FPSH) is globally distributed, forming a distinct lineage across swine-derived samples from South Korea, South Africa, and China. Moreover, mobile genetic element IS Enfa4 , which facilitated horizontal transfer of cfr, was detected downstream of fosS . Notably, various antimicrobial resistance genes (ARGs) were also detected in FPSH. Although fosfomycin was forbidden in food animals in China, the fitness advantages can be conferred by the other ARGs under antimicrobial selective pressure and may facilitate the transmission of FPSH. Overall, this study is the first to report a novel fosfomycin resistance gene and new MurA variants in FRSH. The IS Enfa4 was downstream of fosS and underscored potential mobilization risks. These findings provided a new perspective on One Health to control fosfomycin resistance transmission.","PeriodicalId":74344,"journal":{"name":"One health advances","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onehealthadv.biomedcentral.com/counter/pdf/10.1186/s44280-025-00095-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetics of colistin sulfate and berberine sulfate following intramuscular injection as single agents or in combination in swine","authors":"Jinyan Meng, Ruili Yu, Zeyu Wen, Sumeng Chen, Xiaorong Xie, Lu Zhang, Pan Sun, Xingyuan Cao","doi":"10.1186/s44280-025-00082-2","DOIUrl":"https://doi.org/10.1186/s44280-025-00082-2","url":null,"abstract":"Abstract With the increasing prevalence of drug resistance from antibiotic abuse, bacterial diseases pose a serious threat to the livestock farming industry and public health. This study aimed to investigate and compare the pharmacokinetic profiles of the combined injection comprising colistin sulfate–berberine sulfate and its individual components (colistin sulfate and berberine sulfate) in swine. The combined injection was administered either intramuscularly at doses of 0.5, 1.0, 2.0, and 4.0 mg/kg or intravenously at a dose of 1.0 mg/kg, while single-agent injections were administered intramuscularly at a dose of 2.0 mg/kg. Colistin sulfate and berberine sulfate concentrations in plasma were quantified via ultra-performance liquid chromatography‒tandem Mass spectrometry, and the pharmacokinetic parameters were analyzed via a noncompartmental analysis model. A Linear mixed-effect model was used for the combined injection within the 0.5–4.0 mg/kg range. At the 2.0 mg/kg intramuscular dose, the combination injection produced higher maximum concentration for colistin sulfate (7.00 ± 2.18 μg/mL) and berberine sulfate (3.36 ± 1.04 μg/mL) than the single-agent injections (4.37 ± 0.72 and 1.44 ± 0.32 μg/mL, respectively). Following the combined injection of 0.5, 1.0, 2.0, or 4.0 mg/kg and the single-agent injections of 2.0 mg/kg, the corresponding absolute bioavailabilities were 48.00%, 52.05%, 79.04%, 82.40%, and 77.52%, respectively, for colistin sulfate and 2.98%, 6.81%, 10.49%, 11.35%, and 8.00%, respectively, for berberine sulfate. Furthermore, the combined injection showed dose proportionality following intramuscular administration across the tested dose range for both colistin sulfate and berberine sulfate. Our research findings provide a theoretical basis for the precise clinical use of this drug combination in veterinary medicine and ensure the safety of animal-derived food and public health. Further research is warranted to explore its efficacy and safety in clinical settings.","PeriodicalId":74344,"journal":{"name":"One health advances","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onehealthadv.biomedcentral.com/counter/pdf/10.1186/s44280-025-00082-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of ribosomal protein L16 and development of a tri-mode detection method for amphenicols based on quantum dots and carbon dots","authors":"Yi Cai, Sikun Yuan, Jian Ping Wang, Cheng Feng","doi":"10.1186/s44280-025-00089-9","DOIUrl":"https://doi.org/10.1186/s44280-025-00089-9","url":null,"abstract":"Abstract Amphenicol residues in foods of animal origin can pose severe risks to human health; however, the determination of amphenicols is usually based on a single-mode immunoassays. So a multi-mode method capable of determining amphenicols is desirable. In this study, a tri-mode fluorescence detection method for amphenicols was developed on the basis of the ribosomal protein L16 (rpL16) of Thermus thermophilus . In particular, rpL16 was expressed, and its recognition mechanism for three amphenicols was investigated using a molecular docking technique. The protein was conjugated to red quantum dots to prepare a recognition element, which was combined with a blue carbon dot-labeled fluorescent tracer to develop a direct competitive microplate-based assay for the detection of amphenicols. As the analyte concentration increased, there was a color change from purple to red, the RGB value increased, and the fluorescence intensity from the tracer decreased. The results could be read with the naked eye, a smartphone, and a multimode microplate reader. The limits of detection for determination of the three amphenicols in eggs were 1.0 ng/g via visual detection, 10 pg/g via smartphone detection, and 0.05–0.17 pg/g via instrumental detection. This study reports the first tri-mode detection method for the determination of small molecule compounds based on quantum dots and carbon dots, providing a versatile tool for amphenicol detection in foods of animal origin under different usage scenarios.","PeriodicalId":74344,"journal":{"name":"One health advances","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onehealthadv.biomedcentral.com/counter/pdf/10.1186/s44280-025-00089-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147884031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arginine as host directed therapy in tuberculosis: insights from modulating arginine metabolism by supplementation and arginase inhibition.","authors":"Qingkui Jiang, Ranjeet Kumar, Yi Zhao, Selvakumar Subbian, Lanbo Shi","doi":"10.1186/s44280-025-00070-6","DOIUrl":"10.1186/s44280-025-00070-6","url":null,"abstract":"<p><p>Tuberculosis (TB), caused by <i>Mycobacterium tuberculosis</i> (<i>Mtb</i>), remains a global health challenge. Arginine metabolism is central to immune responses, regulating nitric oxide (NO) production via inducible NO synthase (Nos2) and competing pathways mediated by arginases (Arg1 and Arg2). This study examines the impact of arginine supplementation and arginase inhibition during the acute phase of <i>Mtb</i> infection in mouse lungs, focusing on immune function, lung pathology, and mitochondrial function. Arginine supplementation enhanced Nos2 expression, promoted mitophagy, and supported angiogenesis and/or tissue repair by upregulating <i>Vegfa</i>. These mechanisms synergized to balance pro-inflammatory responses with tissue repair, improving immune defense while mitigating lung damage. In contrast, arginase inhibition disrupted <i>Vegfa</i>-mediated immune homeostasis, and impaired mitophagy, leading to exacerbated lung pathology. These findings underscore the complementary roles of Nos2 and arginase-mediated pathways in maintaining immune equilibrium during <i>Mtb</i> infection. Our results highlight arginine supplementation as a promising host-directed therapy for TB, capable of enhancing protective immunity and facilitating tissue repair. Conversely, caution is warranted for strategies targeting arginase due to potential adverse effects on inflammation resolution and mitochondrial quality control. Future studies should explore the long-term efficacy of arginine-based therapies and their integration with existing antibiotic regimens for optimal TB management.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1186/s44280-025-00070-6.</p>","PeriodicalId":74344,"journal":{"name":"One health advances","volume":"3 1","pages":"5"},"PeriodicalIF":5.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11928424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143694780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selection and characterization of a DNA aptamer for Patulin and its application in a label-free fluorescence assay for fruit juices.","authors":"Sihan Wang, Jiayi Liang, Haiyang Jiang, Jianzhong Shen, Zhanhui Wang, Juewen Liu","doi":"10.1186/s44280-025-00101-2","DOIUrl":"10.1186/s44280-025-00101-2","url":null,"abstract":"<p><p>Patulin (PTL, 154 Da) is a small molecule toxin produced as a mold metabolite. PTL is unstable in animals and is quickly converted to its metabolites. Thus, few successful antibodies were reported even after extensive efforts in hapten design. In this work, a library immobilization strategy was used to obtain DNA aptamers for PTL, where exposure of PTL to live animals or cells is no longer needed. After 20 rounds of selection, the most abundant aptamer named PTL-1 has a dissociation constant (<i>K</i> _d) of 12.5 μM based on isothermal titration calorimetry and 18.4 μM based on thioflavin T (ThT) fluorescence spectroscopy. PTL-1 has excellent selectivity against nine other antibiotics. Compared to previously reported aptamers for PTL, PTL-1 is either shorter or has higher affinity. A label-free ThT fluorescence assay based on PTL-1 was developed, which has a detection limit of 0.2 μM (detection range 0.2-20 μM) for PTL in fruit juices. The PTL-1 aptamer shows resistance to matrix interference and can be applied to the rapid detection of PTL in a blended juice. This study is a nice demonstration of selecting aptamers for a target molecule difficult for the production of antibodies.</p><p><strong>Graphical abstract: </strong></p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1186/s44280-025-00101-2.</p>","PeriodicalId":74344,"journal":{"name":"One health advances","volume":"3 1","pages":"37"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12745320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145866808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid analysis of eight Gelsemium elegans Benth alkaloids in human plasma and urine","authors":"Xi Zeng, Yu Wang, Lin Luo, Yina Lu, Zhenlin Xu","doi":"10.1186/s44280-024-00061-z","DOIUrl":"https://doi.org/10.1186/s44280-024-00061-z","url":null,"abstract":"Abstract Gelsemium elegans Benth alkaloids are the main components of G. elegans and can cause acute toxicosis or even death. Although several studies have reported methods for detecting G. elegans alkaloids, a high-throughput and environmental-friendly strategy for detection of multiple G. elegans alkaloids has not been realized. In this work, a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry method was developed for rapid detection of G. elegans alkaloids in human plasma and urine for diagnosis of poisoning. Multiple matrices and crystal spotting methods were evaluated to obtain stable and high peak intensities without “sweet spot”. We verified the methodology and obtained excellent results. The matrix effects with different dilutions were compared and good recoveries and a low relative standard deviation were obtained with a 40-fold dilution. This method could shorten the analysis time and greatly reduce the consumption of chemical solvents. Furthermore, it could be applied to quantitative assessment of G. elegans alkaloid poisoning incidents.","PeriodicalId":74344,"journal":{"name":"One health advances","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s44280-024-00061-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}