NeuroImmune pharmacology and therapeutics最新文献_第3页

Developmental outcomes with perinatal exposure (DOPE) to prescription opioids. 围产期处方阿片类药物暴露(DOPE)的发育结局。

NeuroImmune pharmacology and therapeutics Pub Date : 2023-11-27 eCollection Date: 2023-12-01 DOI: 10.1515/nipt-2023-0017

Adrian Flores, Nghi M Nguyen, Gurudutt Pendyala

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Harmonizing science with music: a novel approach in AIDS research dissemination 科学与音乐的和谐：传播艾滋病研究成果的新方法

NeuroImmune pharmacology and therapeutics Pub Date : 2023-11-24 DOI: 10.1515/nipt-2023-0023

Kevin Hanrahan

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Blood-brain barrier breakdown in COVID-19 ICU survivors: an MRI pilot study. COVID-19 ICU幸存者血脑屏障破裂:一项MRI试点研究

NeuroImmune pharmacology and therapeutics Pub Date : 2023-11-22 eCollection Date: 2023-12-01 DOI: 10.1515/nipt-2023-0018

Wen Shi, Dengrong Jiang, Hannah Rando, Shivalika Khanduja, Zixuan Lin, Kaisha Hazel, George Pottanat, Ebony Jones, Cuimei Xu, Doris Lin, Sevil Yasar, Sung-Min Cho, Hanzhang Lu

{"title":"Blood-brain barrier breakdown in COVID-19 ICU survivors: an MRI pilot study.","authors":"Wen Shi, Dengrong Jiang, Hannah Rando, Shivalika Khanduja, Zixuan Lin, Kaisha Hazel, George Pottanat, Ebony Jones, Cuimei Xu, Doris Lin, Sevil Yasar, Sung-Min Cho, Hanzhang Lu","doi":"10.1515/nipt-2023-0018","DOIUrl":"10.1515/nipt-2023-0018","url":null,"abstract":"Objectives: Coronavirus disease 2019 (COVID-19) results in severe inflammation at the acute stage. Chronic neuroinflammation and abnormal immunological response have been suggested to be the contributors to neuro-long-COVID, but direct evidence has been scarce. This study aims to determine the integrity of the blood-brain barrier (BBB) in COVID-19 intensive care unit (ICU) survivors using a novel MRI technique.Methods: COVID-19 ICU survivors (n=7) and age and sex-matched control participants (n=17) were recruited from June 2021 to March 2023. None of the control participants were hospitalized due to COVID-19 infection. The COVID-19 ICU survivors were studied at 98.6 ± 14.9 days after their discharge from ICU. A non-invasive MRI technique was used to assess the BBB permeability to water molecules, in terms of permeability surface area-product (PS) in the units of mL/100 g/min.Results: PS was significantly higher in COVID-19 ICU survivors (p=0.038) when compared to the controls, with values of 153.1 ± 20.9 mL/100 g/min and 132.5 ± 20.7 mL/100 g/min, respectively. In contrast, there were no significant differences in whole-brain cerebral blood flow (p=0.649) or brain volume (p=0.471) between the groups.Conclusions: There is preliminary evidence of a chronic BBB breakdown in COVID-19 survivors who had a severe acute infection, suggesting a plausible contributor to neurological long-COVID symptoms.","PeriodicalId":74278,"journal":{"name":"NeuroImmune pharmacology and therapeutics","volume":"2 4","pages":"333-338"},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10696574/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138500418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activation of CB2R by synthetic CB2R agonist, PM289, improves brain endothelial barrier properties, decreases inflammatory response and enhances endothelial repair 通过合成CB2R激动剂PM289激活CB2R，可以改善脑内皮屏障特性，减少炎症反应，增强内皮修复

NeuroImmune pharmacology and therapeutics Pub Date : 2023-10-16 DOI: 10.1515/nipt-2023-0016

Trent A. Bullock, Kalpani N. Udeni Galpayage Dona, Jonathan F. Hale, Paula Morales, Nadine Jagerovic, Allison M. Andrews, Servio H. Ramirez

{"title":"Activation of CB2R by synthetic CB2R agonist, PM289, improves brain endothelial barrier properties, decreases inflammatory response and enhances endothelial repair","authors":"Trent A. Bullock, Kalpani N. Udeni Galpayage Dona, Jonathan F. Hale, Paula Morales, Nadine Jagerovic, Allison M. Andrews, Servio H. Ramirez","doi":"10.1515/nipt-2023-0016","DOIUrl":"https://doi.org/10.1515/nipt-2023-0016","url":null,"abstract":"Abstract The Cannabinoid 2 Receptor (CB2R) has been found to provide immunological modulation in different cell types. More recently, detection of CB2R in the cerebral endothelium suggests a possible role in the resolution of inflammation at the level of the blood–brain–barrier (BBB). Here, the notion that CB2R upregulation in brain endothelial cells could be exploited to promote vascular protection and BBB integrity was evaluated. Targeting and activation of CB2R was accomplished by a novel and highly specific chromenopyrazole based CB2R agonist, PM289. This study demonstrates that CB2R upregulation is induced as early as 8 h in the cortical vasculature in an experimental mouse model of TBI. Unlike CB2R, CB1R was marginally detected and not significantly induced. In the human brain endothelial cell line, hCMEC/D3 cells, similar induction of CB2R was observed upon stimulation with TNFα. Analysis of transendothelial electrical resistance shows that PM289 markedly prevented the barrier-leakiness induced by TNFα. The BBB is also responsible for maintaining an immunological barrier. The five-fold increase in ICAM1 expression in stimulated endothelial cells was significantly diminished due to CB2R activation. Utilizing wounding assays, results showed that wound repair could be accomplished in nearly half the time when the novel CB2R agonist is present compared to the untreated control. Lastly, mechanistically, the effects of CB2R may be explained by the observed inhibition of the p65 NFκB subunit. Overall, these studies support the notion that targeting and activating CB2R in the brain vasculature could aid in BBB and vascular protection in the context of neuroinflammation.","PeriodicalId":74278,"journal":{"name":"NeuroImmune pharmacology and therapeutics","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136078247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Prenatal and adolescent alcohol exposure, neuroinflammation, and Alzheimer’s disease: a network meta analysis approach 产前和青少年酒精暴露，神经炎症和阿尔茨海默病:网络meta分析方法

NeuroImmune pharmacology and therapeutics Pub Date : 2023-09-06 DOI: 10.1515/nipt-2023-0003

Lazer Gerlikhman, Ujjal Das, Dipak K. Sarkar

{"title":"Prenatal and adolescent alcohol exposure, neuroinflammation, and Alzheimer’s disease: a network meta analysis approach","authors":"Lazer Gerlikhman, Ujjal Das, Dipak K. Sarkar","doi":"10.1515/nipt-2023-0003","DOIUrl":"https://doi.org/10.1515/nipt-2023-0003","url":null,"abstract":"Abstract Objectives This review aims to determine the connection between developmental alcohol exposure and its potential impact on Alzheimer's disease (AD) later in life. We employ a network meta-analysis approach and examine gene fold changes from literature and Gene Expression Omnibus (GEO) datasets. Our goal is to investigate whether prenatal alcohol exposure (PAE) and/or adolescent alcohol exposure (AAE) could activate specific neuroinflammatory genes, potentially increasing the risk of AD development. Content We conducted a comprehensive analysis of brain datasets using a network meta-analysis approach. By synthesizing gene fold changes from literature and GEO datasets, we examined the potential impact of developmental alcohol exposure on increased risk of developing AD in the future. Summary Our findings reveal significant associations between alcohol exposure and critical functional categories and diseases in the brain. Alcohol exposure was strongly linked to the “Inflammatory Response” and “Nervous System Development and Function” categories, indicative of inflammatory reactions in the brain and detrimental effects on nervous system integrity. Furthermore, we observed links with “Organismal Injury and Abnormalities” and “Cell Death and Survival.” Pathway analysis revealed dysregulation in neuroinflammatory, ERK/MAPK signaling, amyloid processing, IL-1 signaling and calcium signaling pathways, suggesting their potential involvement in alcohol-induced neurotoxicity. Outlook This review highlights the necessity of recognizing developmental alcohol exposure as a potential risk factor for AD and shed light on the underlying mechanisms that may contribute to alcohol-induced neurotoxicity. By expanding our understanding of these mechanisms, we can better address the complex relationship between developmental alcohol exposure and neurodegenerative disorders like AD.","PeriodicalId":74278,"journal":{"name":"NeuroImmune pharmacology and therapeutics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43310333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Frontmatter 头版头条

NeuroImmune pharmacology and therapeutics Pub Date : 2023-09-01 DOI: 10.1515/nipt-2023-frontmatter3

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Immune senescence in aged APP/PS1 mice. 老年APP/PS1小鼠的免疫衰老

NeuroImmune pharmacology and therapeutics Pub Date : 2023-08-14 eCollection Date: 2023-09-01 DOI: 10.1515/nipt-2023-0015

Mai M Abdelmoaty, Pravin Yeapuri, Jatin Machhi, Yaman Lu, Krista L Namminga, Rana Kadry, Eugene Lu, Shaurav Bhattarai, Rodney Lee Mosley, Howard E Gendelman

{"title":"Immune senescence in aged APP/PS1 mice.","authors":"Mai M Abdelmoaty, Pravin Yeapuri, Jatin Machhi, Yaman Lu, Krista L Namminga, Rana Kadry, Eugene Lu, Shaurav Bhattarai, Rodney Lee Mosley, Howard E Gendelman","doi":"10.1515/nipt-2023-0015","DOIUrl":"10.1515/nipt-2023-0015","url":null,"abstract":"Objectives: To evaluate the linkage between age and deficits in innate and adaptive immunity which heralds both Alzheimer's disease (AD) onset and progression. The pathobiological events which underlie and tie these outcomes remain not fully understood.Methods: To investigate age-dependent immunity in AD, we evaluated innate and adaptive immunity in coordinate studies of regulatory T cell (Treg) function, T cell frequencies, and microglial integrity. These were assessed in blood, peripheral lymphoid tissues, and the hippocampus of transgenic (Tg) amyloid precursor protein/presenilin 1 (APP/PS1) against non-Tg mice. Additionally, immune arrays of hippocampal tissue were performed at 4, 6, 12, and 20 months of age.Results: APP/PS1 mice showed progressive impairment of Treg immunosuppressive function with age. There was partial restoration of Treg function in 20-month-old mice. Ingenuity pathway analyses of hippocampal tissues were enriched in inflammatory, oxidative, and cellular activation pathways that paralleled advancing age and AD-pathobiology. Operative genes in those pathways included, but were not limited to triggering receptor on myeloid cells 1 (TREM1), T helper type 1 (Th1), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathways. Interleukin-17 (IL-17), nitric oxide, acute phase, and T cell receptor signaling pathways were also perturbed. Significant inflammation was observed at 6- and 12-months. However, at 20-months, age associated partial restoration of Treg function reduced inflammatory phenotype.Conclusions: Impaired Treg function, inflammation and oxidative stress were associated with AD pathology. Age associated partial restoration of Treg function in old mice reduced the hippocampal inflammatory phenotype. Restoring Treg suppressive function can be a therapeutic modality for AD.","PeriodicalId":74278,"journal":{"name":"NeuroImmune pharmacology and therapeutics","volume":" ","pages":"317-330"},"PeriodicalIF":0.0,"publicationDate":"2023-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10659760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46909718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple protocol for isolating microglia from adult mouse brain. 从成年小鼠大脑中分离小胶质细胞的简单方法

NeuroImmune pharmacology and therapeutics Pub Date : 2023-08-02 eCollection Date: 2023-09-01 DOI: 10.1515/nipt-2023-0014

Sudipta Chakrabarti, Sukhamoy Gorai, Kalipada Pahan

{"title":"A simple protocol for isolating microglia from adult mouse brain.","authors":"Sudipta Chakrabarti, Sukhamoy Gorai, Kalipada Pahan","doi":"10.1515/nipt-2023-0014","DOIUrl":"10.1515/nipt-2023-0014","url":null,"abstract":"Objectives: Although microglia are activated in adult and aged brains resulting in neurodegenerative and neuroinflammatory disorders, most of the cell culture studies on microglia deal with neonatal microglia because of ease of isolation. Microglia could be isolated from adult brains, but it requires separation by density gradient centrifugation, magnetic beads, etc. Here, we describe a simple protocol of isolating highly purified microglia from adult mouse brains.Methods: Our protocol involves dilution with sterile PBS or media, regular centrifugation, and plating on poly-D-lysine-coated flasks.Results: These adult microglia expressed the inducible nitric oxide synthase in response to preformed α-syn fibril, an etiological reagent of Parkinson's disease, and bacterial lipopolysaccharides, one of the prototype proinflammatory stimuli. Moreover, these adult microglia exhibited phagocytosis, which was stimulated by LPS treatment.Conclusions: These results suggest that adult microglia isolated by our procedure are functional and that these adult microglia could be used for studies related to neurodegenerative disorders.","PeriodicalId":74278,"journal":{"name":"NeuroImmune pharmacology and therapeutics","volume":"2 1","pages":"293-300"},"PeriodicalIF":0.0,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10474378/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48987881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Substances of abuse and their effect on SAR-CoV-2 pathogenesis. 滥用物质及其对严重急性呼吸系统综合征冠状病毒2型发病机制的影响

NeuroImmune pharmacology and therapeutics Pub Date : 2023-07-31 eCollection Date: 2023-09-01 DOI: 10.1515/nipt-2023-0004

Ivy Antwi, Destiny Watkins, Alahn Pedawi, Atheel Ghrayeb, Christine Van de Vuurst, Theodore J Cory

{"title":"Substances of abuse and their effect on SAR-CoV-2 pathogenesis.","authors":"Ivy Antwi, Destiny Watkins, Alahn Pedawi, Atheel Ghrayeb, Christine Van de Vuurst, Theodore J Cory","doi":"10.1515/nipt-2023-0004","DOIUrl":"10.1515/nipt-2023-0004","url":null,"abstract":"Following the emergence of SARS-CoV-2, various reports suggest that there has been a significant increase in substance abuse due to social distancing and related issues. Several reports have suggested the impact of chronic substance use on individuals' physiological and psychological health. Therefore, there is a need to know the impact of SARS-CoV-2 on persons with substance use disorders. Individuals with substance use disorders are the most vulnerable groups and are at a high risk of SARS-CoV-2 infection due to their already existing health issues associated with substance use. This review discusses some of the molecular and systemic/organic effects chronic substance use such as alcohol, nicotine, marijuana (cannabis), opioids, methamphetamine, and cocaine have on SARS-CoV-2 infectivity and its potential cause for worsened disease outcomes in persons with substance use disorder. This will provide healthcare providers, public health policies, and researchers with the needed knowledge to address some of the many challenges faced during the Covid-19 pandemic to facilitate treatment strategies for persons with substance use disorders.","PeriodicalId":74278,"journal":{"name":"NeuroImmune pharmacology and therapeutics","volume":"2 1","pages":"301-316"},"PeriodicalIF":0.0,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10474379/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47402015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Poor subjective sleep reported by people living with HIV is associated with impaired working memory. HIV感染者报告的主观睡眠不足与工作记忆受损有关

NeuroImmune pharmacology and therapeutics Pub Date : 2023-07-20 Epub Date: 2023-07-11 DOI: 10.1515/nipt-2023-0010

Natalie M Zahr, Edith V Sullivan, Adolf Pfefferbaum

{"title":"Poor subjective sleep reported by people living with HIV is associated with impaired working memory.","authors":"Natalie M Zahr, Edith V Sullivan, Adolf Pfefferbaum","doi":"10.1515/nipt-2023-0010","DOIUrl":"10.1515/nipt-2023-0010","url":null,"abstract":"Poor sleep can undermine health and may be especially disruptive to those with chronic conditions including HIV infection. Here, clinically well-described people living with HIV [PLWH] (74 men, 35 women) and healthy control (38 men, 35 women) participants were administered the Pittsburgh Sleep Quality Index (PSQI), a validated measure of subjective sleep with a global score ≥5 able to distinguish good from poor sleepers. In addition, participants completed a battery of neuropsychological tests. PLWH (6.8 ± 3.7) had higher global PSQI scores than healthy controls (4.1 ± 2.8): 39.7 % of uninfected controls and 68.8 % of PLWH had a PSQI≥5 indicative of poor sleep. There were no relations between the global PSQI score and any evaluated variables among uninfected individuals or with demographic or HIV-related variables in PLWH. Instead, a higher global PSQI score among PLWH was associated with worse \"Quality of Life\" scores [Global Assessment of Functioning (GAF, p=0.0007), Medical Outcomes Study survey (21-item short form, SF-21, p<0.0001), and Activities of Daily Living-Instrumental (ADL-I, p=0.0041)] and higher Beck Depression Index (BDI, p<0.0001) depressive symptoms. Further, in PLWH, higher global PSQI scores were associated with poor performance on a working memory task, the digit backward span (p=0.0036). In PLWH, the 5 variables together explained 32.3 % of the global PSQI score variance; only 3 variables - the SF-21, BDI, and digit backward scores - explained 30.6 % of the variance. To the extent that poor subjective sleep contributes to impaired working memory in HIV, we speculate that this impairment may be ameliorated by improved sleep health.","PeriodicalId":74278,"journal":{"name":"NeuroImmune pharmacology and therapeutics","volume":"2 1","pages":"127-137"},"PeriodicalIF":0.0,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10635409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46212192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0