Mycobacterial diseases : tuberculosis & leprosy最新文献_第7页

Current Overview of Anti-Tuberculosis Drugs: Metabolism and Toxicities 抗结核药物的现状综述:代谢和毒性

Mycobacterial diseases : tuberculosis & leprosy Pub Date : 2016-05-26 DOI: 10.4172/2161-1068.1000209

Susmita Sarkar, Advaita Ganguly, H. Sunwoo

引用次数: 38

Expression and Analysis of CXCL8 and CXCL10 Chemokines in Human SkinLesions Infected with M.leprae 麻疯病人皮肤病变中CXCL8和CXCL10趋化因子的表达及分析

Mycobacterial diseases : tuberculosis & leprosy Pub Date : 2016-05-24 DOI: 10.4172/2161-1068.1000208

K. Rawat, M. Chahar, P. Reddy, N. Srivastava, U. Gupta, M. Natrajan, V. Katoch, K. Katoch, D. Chauhan

{"title":"Expression and Analysis of CXCL8 and CXCL10 Chemokines in Human SkinLesions Infected with M.leprae","authors":"K. Rawat, M. Chahar, P. Reddy, N. Srivastava, U. Gupta, M. Natrajan, V. Katoch, K. Katoch, D. Chauhan","doi":"10.4172/2161-1068.1000208","DOIUrl":"https://doi.org/10.4172/2161-1068.1000208","url":null,"abstract":"This study is focused to analyze the expression profile of CXCL8 and CXCL10 chemokines genes and to address the contribution of these chemokines in late phase of M.leprae infection in human skin lesion samples. In this study skin biopsy samples from leprosy patients (n=35) were collected including BL-LL=12, BB=14, BT=8 and healthy volunteers (n=3). All the biopsy samples were collected in RNALater for mRNA expression study as well as 10% buffered formalin (BF) for histopathologic analysis. Total RNA was isolated from collected samples and cDNA was prepared. Level of mRNA expression of CXCL8 and CXCL10 chemokine genes was measured via q-PCR and insitu RT-PCR to locate the presence of CXCL8 and CXCL10 chemokines inside M. leprae infected tissue. The mRNA expression of CXCL8 was found relatively elevated in lepromatous (BL-LL) skin samples significantly as compared to BB category of leprosy; whereas in BT cases down-regulation was recorded. CXCL10 mRNA expression was found elevated in lepromatous (BL-LL) cases than other cases (BT, BB) of leprosy samples. In histopathological examination, 59.38% maximum infiltration was observed in BL-LL cases of leprosy and in-situ RT-PCR confirmed the presence of chemokines genes in tissue sections. Interestingly, chemokines CXCL-10 and CXCL-8 showed elevated expression in BL-LL category. The study advocated that CXCL10 and CXCL-8 possibly may have a role in lepromatous (BL-LL) form of leprosy.","PeriodicalId":74235,"journal":{"name":"Mycobacterial diseases : tuberculosis & leprosy","volume":"173 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2016-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2161-1068.1000208","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70583464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Extending a Step Further: Offering Tobacco Cessation Counselling to Contacts ofTuberculosis Patients 更进一步:向结核病患者接触者提供戒烟咨询

Mycobacterial diseases : tuberculosis & leprosy Pub Date : 2016-05-16 DOI: 10.4172/2161-1068.1000E131

P. Chavda, K. Mehta

引用次数: 1

Minimally Invasive Ultrasound-guided Synovial Biopsy Using SuperCoreBiopsy Instrument 超声引导下使用SuperCoreBiopsy仪器进行滑膜微创活检

Mycobacterial diseases : tuberculosis & leprosy Pub Date : 2016-05-14 DOI: 10.4172/2161-1068.1000207

K. Lai, Hsin-Hua Chen, M. Wen, Yi-Ming Chen, J. Lan, Der-Yuan Chen

{"title":"Minimally Invasive Ultrasound-guided Synovial Biopsy Using SuperCoreBiopsy Instrument","authors":"K. Lai, Hsin-Hua Chen, M. Wen, Yi-Ming Chen, J. Lan, Der-Yuan Chen","doi":"10.4172/2161-1068.1000207","DOIUrl":"https://doi.org/10.4172/2161-1068.1000207","url":null,"abstract":"Background: To develop a new method for synovial biopsy with ultrasound (US) guidance and a semi-automatic SuperCore biopsy instrument. \u0000Materials and Methods: Twenty-two patients (8 men and 14 women, median age 57 years (range 22–79 years)) with active arthritis or tenosynovitis were enrolled from April 2012 through October 2012. Each patient had one joint or tendon biopsied. US examination was performed to determine the optimal synovial site for biopsy. After skin disinfection and local anaesthesia, a portal was established using a trephine needle as needed. An 18-gauge SuperCore biopsy needle was placed into the target synovial site via the portal or just percutaneously under US guidance with free hand technique. Repeated needle passes and cuts to obtain 3-10 pieces of synovial tissues in each joint. The success of biopsy was defined as identification of synovium on histological examination. \u0000Results: Synovium of 21 joints (10 knees, 6 wrists, 3 ankles, 1 elbow and 1 metacarpophalangeal joint) were biopsied. One patient had biopsy of flexor digitorum tendon sheath. All biopsies obtained adequate amounts of synovial tissues for histologic reading with a success rate of 100%. Synovial lining was identified in 18 (85.7%) of 21 joints. The patient with flexor digitorum tenosynovitis was proven to have mycobacterial infectionby histological examination. All patients tolerated the procedures well, and no complication was observed during 2-week follow up. \u0000Conclusions: US-guided synovial biopsy using SuperCore biopsy instrument is a promising method for synovial research. It has the advantages of simple, mini-invasiveness and high success rate. The complication is rare.","PeriodicalId":74235,"journal":{"name":"Mycobacterial diseases : tuberculosis & leprosy","volume":"6 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2016-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2161-1068.1000207","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70583356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Rv3802c in Tuberculosis Therapeutics Rv3802c在结核病治疗中的应用

Mycobacterial diseases : tuberculosis & leprosy Pub Date : 2016-04-25 DOI: 10.4172/2161-1068.1000204

P. Saravanan, S. Patra

引用次数: 3

Fulminant Pulmonary Tuberculosis by Infliximab in Patient withRheumatoid Arthritis 英夫利昔单抗治疗类风湿关节炎患者的暴发性肺结核

Mycobacterial diseases : tuberculosis & leprosy Pub Date : 2016-04-23 DOI: 10.4172/2161-1068.1000206

M. Hama, Y. Yamazaki, M. Kosaka, A. Ushiki, N. Goto, M. Sugawara, M. Hanaoka

引用次数: 3

The Yield of First Spot Double Slide Smears for the Diagnosis ofPulmonary Tuberculosis 首点双片涂片诊断肺结核的产率

Mycobacterial diseases : tuberculosis & leprosy Pub Date : 2016-04-22 DOI: 10.4172/2161-1068.1000205

B. Ayele

{"title":"The Yield of First Spot Double Slide Smears for the Diagnosis ofPulmonary Tuberculosis","authors":"B. Ayele","doi":"10.4172/2161-1068.1000205","DOIUrl":"https://doi.org/10.4172/2161-1068.1000205","url":null,"abstract":"Background: Direct sputum smear microscopy is the cornerstone for the diagnosis of pulmonary tuberculosis (PTB) in resource-poor countries. However, the requirement for repeated visits to submit specimens and receive results is associated with considerable diagnostic delay, work load, patients drop-out and high expenses for patients. Although the World Health Organization (WHO) has recently changed its policy from spot morning spot (SMS) to spot morning (SM), the SM method still involves two days visits for a patient. The aim of this study was to evaluate the yield of first spot double slides smears for the diagnosis of PTB in high TB setting. \u0000Methods: A total of 362 patients who visited the out-patient department (OPD) of Dilla referral hospital and who were suspected of PTB were involved in the study. In addition SMS sputum samples were collected; double slides smears were prepared, stained by the Ziehl-Neelsen (ZN) method and 100 fields were examined under oil immersion objective for acid fast bacilli (AFB). \u0000Results: Of 362, 54 (14.92%) were smear-positives. Out of the 54 smear positive patients, 53 (98.15%) were positives by the first spot specimen. Additionally, 1 of 54 (1.85%) were positives by the morning specimen. Using the 2-day protocol (SMS) among 362 patients, 54 (14.92%) were smear positives by double slides and 53 (14.64%) by single slide smears. Whereas using the 1-day protocol (first spot); among 362 patients, 53 (14.64%) were smear positives both in double slides and single slide smears. \u0000Conclusion: The double slides smears from the first spot sputum samples appeared to be as effective as SMS strategy for the diagnosis of PTB though additional studies are required under various settings.","PeriodicalId":74235,"journal":{"name":"Mycobacterial diseases : tuberculosis & leprosy","volume":"6 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2016-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70583103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Thiopeptide Antibiotics act on both Host and Microbe to Deliver Double Punch on Mycobacterial Infection 硫肽类抗生素同时作用于宿主和微生物，对分枝杆菌感染产生双重作用

Mycobacterial diseases : tuberculosis & leprosy Pub Date : 2016-03-25 DOI: 10.4172/2161-1068.1000203

Qingfei Zheng, Wen Liu

引用次数: 4

Computational Approach in Understanding Mechanism of Action of Isoniazidand Drug Resistance 理解异烟肼和耐药作用机制的计算方法

Mycobacterial diseases : tuberculosis & leprosy Pub Date : 2016-03-22 DOI: 10.4172/2161-1068.1000202

Lingaraja Jena, G. Wankhade, Pranita J Waghmare, B. Harinath

{"title":"Computational Approach in Understanding Mechanism of Action of Isoniazidand Drug Resistance","authors":"Lingaraja Jena, G. Wankhade, Pranita J Waghmare, B. Harinath","doi":"10.4172/2161-1068.1000202","DOIUrl":"https://doi.org/10.4172/2161-1068.1000202","url":null,"abstract":"Most Multi Drug Resistance and Extremely Drug Resistance clinical strains of Mycobacterium tuberculosis are found to be resistant to the anti-tuberculousis drugs such as Isoniazid and Rifampicin. The mechanism of action and drug resistance due to Isoniazid has been the subject of extensive study. According to Tuberculosis drug resistance mutation database, 22 genes/proteins are associated with Isoniazid resistance such as katG, nat, inhA, ahpc, ndh, kasA etc. Mutation in the gene seems to affect the formation of Isoniazid to its active form or enhancing the catabolism thus making it ineffective. Studies in different laboratories have shown usefulness of computational approach in elucidating the mechanism of action of Isoniazid and development of drug resistance. Computational studies in our laboratory showed that a mutation in KatG (S315T/S315N) prevents free radical formation, thus the development of resistance to the drug. Further, we observed through molecular dynamics simulation approach that mutation (G67R/G207E) in NAT enzyme increases the stability and catalytic ability of the mutant enzyme, thus making the drug ineffective.","PeriodicalId":74235,"journal":{"name":"Mycobacterial diseases : tuberculosis & leprosy","volume":"6 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2016-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70582468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Detection of Mycobacterium Gilvum First Time from the Bathing Water of Leprosy Patient from Purulia, West Bengal 西孟加拉邦普鲁里亚麻风病人洗浴水中首次检出Gilvum分枝杆菌

Mycobacterial diseases : tuberculosis & leprosy Pub Date : 2016-03-21 DOI: 10.4172/2161-1068.1000201

M. Lavania, R. Turankar, I. Singh, A. Nigam, U. Sengupta

引用次数: 4