Mycobacterial diseases : tuberculosis & leprosy最新文献_第2页

Use of Ultrasonication as a Rapid Pretreatment Method for MALDI-TOF MS of Mycobacterial Samples 超声法快速预处理分枝杆菌MALDI-TOF质谱法

Mycobacterial diseases : tuberculosis & leprosy Pub Date : 2018-05-07 DOI: 10.4172/2161-1068.1000260

K. Niitsuma, S. Koshiba, M. Saitou, Tomoko Suzuki, K. Chikamatsu, Akiko Takagi, S. Mitarai

{"title":"Use of Ultrasonication as a Rapid Pretreatment Method for MALDI-TOF MS of Mycobacterial Samples","authors":"K. Niitsuma, S. Koshiba, M. Saitou, Tomoko Suzuki, K. Chikamatsu, Akiko Takagi, S. Mitarai","doi":"10.4172/2161-1068.1000260","DOIUrl":"https://doi.org/10.4172/2161-1068.1000260","url":null,"abstract":"Background: In Japan, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is used as a simple and accurate method for mycobacterial identification, but existing pretreatment techniques are time-consuming and laborious.Objective: We characterized a new pretreatment technique that extracts proteins using ultrasonic disruption.Methods: We compared this new technique with the current pretreatment method and examined the usefulness of ultrasonication, including its use in mycobacterial inactivation. A total of 174 mycobacterial isolates were tested, including 50 Mycobacterium tuberculosis complex strains, 57 Mycobacterium avium strains, 55 Mycobacterium intracellulare strains, and 12 Mycobacterium kansasii strains. The ultrasonic pretreatment method was performed with or without heat-pretreatment at 95°C for 30 minutes, in parallel with the current conventional method. For all tested strains, the mycobacterial identification agreed when comparing the new and conventional methods.Results: Samples prepared by ultrasonication without heat pretreatment exhibited multiple significant differences when compared with samples prepared by ultrasonication with heat pretreatment or by conventional methods. However, most scores were over 2.0, and the lowest score exceeded 1.7. Furthermore, the new techniques could be performed in only 10 minutes for diagnosis. In addition, we confirmed that 1 minute of ultrasonic pretreatment yielded complete inactivation of M. tuberculosis complex strains.Conclusion: The more rapid technique of protein extraction/inactivation by ultrasonication without heat pretreatment is expected to be highly useful in clinical laboratory settings.","PeriodicalId":74235,"journal":{"name":"Mycobacterial diseases : tuberculosis & leprosy","volume":"8 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2018-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45365840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

First Insight into Molecular Epidemiology of Tuberculosis Infection in Slaughtered Sheep Intended to Human Consumption in Cameroon: The Case of New-Bell’s Slaughterhouses 喀麦隆供人类食用的屠宰绵羊结核病感染的分子流行病学初探——以新贝尔屠宰场为例

Mycobacterial diseases : tuberculosis & leprosy Pub Date : 2018-04-23 DOI: 10.4172/2161-1068.1000259

F. Koro, Ateugieu Guemechieu Romaric, Em Tchamba Kombou, O. Tatiana, Yannick Kamdem Simo, J. A. Assam, Penlap Beng, N. N. Rosalie, E. F. Xavier

{"title":"First Insight into Molecular Epidemiology of Tuberculosis Infection in Slaughtered Sheep Intended to Human Consumption in Cameroon: The Case of New-Bell’s Slaughterhouses","authors":"F. Koro, Ateugieu Guemechieu Romaric, Em Tchamba Kombou, O. Tatiana, Yannick Kamdem Simo, J. A. Assam, Penlap Beng, N. N. Rosalie, E. F. Xavier","doi":"10.4172/2161-1068.1000259","DOIUrl":"https://doi.org/10.4172/2161-1068.1000259","url":null,"abstract":"Objective: Tuberculosis (TB) remains understudied in Cameroonian sheep which are very consumed by human beings and which nevertheless live in close contact with cattle in which TB remains endemic. We carried for the first time a six months descriptive cross sectional study on slaughtered sheep in the major slaughter-house of Douala, from November 2013 to April 2014.Methods: About 2922 slaughtered sheep were successively inspected for tuberculosis through visual examination and palpation of carcasses, lungs, livers, hearts, intestine, inner carcasses and lymph nodes. ZiehlNeelsen staining, culture on Lowenstein Jensen solid media supplemented with Pyruvate or Para Nitro Benzene (PNB) and molecular techniques (Genotype Mycobacterium CM/AS assay and Spoligotyping) were used to identify atypical mycobacteria and Mycobacterium tuberculosis Complex (MTBC) species.Results: From the 2922 sheep slaughtered, 810 presented tuberculosis like lesions corresponding to an apparent prevalence of 27.72% (810/2922). Ziehl-Neelsen examination confirmed Acid fast bacilli in 77.78% (630/810) of cases, corresponding to apparent prevalence of 21.56%. Liver was significantly the most affected by tuberculosis like lesion with 40.74% of case (χ2=67.02, p<0.0001). The use of para-nitro-benzoic acid showed that 10.74% of all detected cases are caused by atypical mycobacteria, for which molecular typing identified, Mycobacterium fortuitum, Mycobacterium interjectum and Mycobacterium sp. Moreover, spoligotyping reveals that 4.44% of cases were due to M. tuberculosis represented by it ubiquitous T lineage SIT53. No M. bovis or M. caprae were identified.Conclusion: This result shows implication of M. tuberculosis and the high prevalence of atypical mycobacteria in sheep intended for human consumption in Cameroon.","PeriodicalId":74235,"journal":{"name":"Mycobacterial diseases : tuberculosis & leprosy","volume":" ","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2018-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47621991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strategies for Reducing Leprosy Stigma 减少麻风污名化战略

Mycobacterial diseases : tuberculosis & leprosy Pub Date : 2018-02-02 DOI: 10.4172/2161-1068.1000253

C. Castro, Lina Erazo, M. Gunturiz

引用次数: 3

Mitigating PCR /Amplicon Contamination in a High Risk High Burden Mycobacterial Reference Laboratory in a Resource Limited Setting 在资源有限的高风险高负担分枝杆菌参比实验室减轻PCR /扩增子污染

Mycobacterial diseases : tuberculosis & leprosy Pub Date : 2018-01-01 DOI: 10.4172/2161-1068.1000261

P. Das, Somtirtha B Ganguly, Bodhisatya Mandal

{"title":"Mitigating PCR /Amplicon Contamination in a High Risk High Burden Mycobacterial Reference Laboratory in a Resource Limited Setting","authors":"P. Das, Somtirtha B Ganguly, Bodhisatya Mandal","doi":"10.4172/2161-1068.1000261","DOIUrl":"https://doi.org/10.4172/2161-1068.1000261","url":null,"abstract":"Background: Nucleic acid amplification techniques have become important machineries in the diagnosis of several diseases in clinical laboratories. PCR contamination/Amplicon Contamination leading to false positivity remains a major concern in these laboratories. Prevention of these contaminations in establishing these Molecular Biology Laboratories has been very crucial over the years. Though closed system PCRs has substantial reduction in the PCR contamination rates the conventional probe based hybridization methods continues to show occurrence of contamination for various reasons. The Study involved checking the crucial parameters as well as the probable candidates of causing the contamination at a high burden setting. Bringing out the most effective interventions in controlling PCR contaminations for future endeavors stood as a priority. The study explored the efficacies of different sets of interventions contributed in the process of reducing the contaminants.Materials and Methods: The detection of the contaminating PCR products or amplicons or contaminating organism is done by the Genotype MTBDR plus V2 kits (Hains Life Sciences) based on DNA strip technologyResults: The pre and post cleaning as well as cleaning of the working surfaces was able to bring down the mean contamination percentage by 36.5%. The combined effect of the cleaning of the work surfaces, the automated pipetting devices and the AC machines along with it filters were able bring down the mean contamination percentage to 53.5% reducing the rate contamination nearly to between 94.6% (mean percentage contamination was 56.5% at the control run).","PeriodicalId":74235,"journal":{"name":"Mycobacterial diseases : tuberculosis & leprosy","volume":"8 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70585114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Potentially Pathogenic Non-Tuberculous Mycobacteria in Artisanal Coalho Cheese from the State of Paraiba, Northeast Brazil 巴西东北部帕拉伊巴州手工奶酪中潜在致病性非结核分枝杆菌的检测

Mycobacterial diseases : tuberculosis & leprosy Pub Date : 2018-01-01 DOI: 10.4172/2161-1068.1000266

Sarti Ecfb, Rodrigues Ra, Duarte Lfc, Bacanelli Gm, W. Lilenbaum, Araujo Fr

引用次数: 2

Abdominal Wall Tuberculosis Infection and TB Peritonitis Seen in 18F-FDG PET/CT Imaging 18F-FDG PET/CT表现为腹壁结核感染和结核性腹膜炎

Mycobacterial diseases : tuberculosis & leprosy Pub Date : 2018-01-01 DOI: 10.4172/2161-1068.1000254

P. Hsiao, TaoQian Tang, S. Liu, Jen-Jyh Lee

引用次数: 0

Strategies for Designing Novel Anti-Tubercular Drugs with Special Reference to Mycobacterial MelF (Rv1936) as a Target 以分枝杆菌MelF (Rv1936)为靶点的新型抗结核药物的设计策略

Mycobacterial diseases : tuberculosis & leprosy Pub Date : 2018-01-01 DOI: 10.4172/2161-1068.1000262

R. Dharra, Mehta Pk

引用次数: 1

Screening of BCG Vaccine Efficacy among Healthy Vaccinated Adults in Khartoum, Sudan 在苏丹喀土穆接种卡介苗的健康成人中筛选卡介苗的效力

Mycobacterial diseases : tuberculosis & leprosy Pub Date : 2018-01-01 DOI: 10.4172/2161-1068.1000269

Goris Bmt, Saleh Fm, Mohammed Fa, Mohammed Sa, Ahmed, Yousif Ra, M. Mi

{"title":"Screening of BCG Vaccine Efficacy among Healthy Vaccinated Adults in Khartoum, Sudan","authors":"Goris Bmt, Saleh Fm, Mohammed Fa, Mohammed Sa, Ahmed, Yousif Ra, M. Mi","doi":"10.4172/2161-1068.1000269","DOIUrl":"https://doi.org/10.4172/2161-1068.1000269","url":null,"abstract":"Tuberculosis (TB) is a major global health problem. The BCG is an attenuated vaccine. Tuberculin test is used as a nonspecific TB vaccination indicator. This study was aimed to determine the efficacy of BCG vaccine through screening of healthy, vaccinated adults’ subjects in Khartoum. A total of one hundred (n=100) healthy and TB symptoms free participants were screened by manteaux test. The participants were involving 55 (55%) males and 45 (45%) female. All participants were over 20 years and most of them had a scar in their vaccination site. All participants were screened through injection of purified protein derivative PPD (only 0.1 mL) intra-dermally into their volar forearm then 48-72 post-injection their induration diameter was measured. The results showed that out one hundred (n=100) participants, only 39 (39%) were positive for Manteaux test (≥ 10 mm diameter), while 61 (61%) were negative (≥ 10 mm diameter). Among the 39 positives, 33 show reading between 10 mm to 15 mm and 6 of them show zone ≥ 15 mm. In the other hand 53 out of 61 tuberculin test negative participants showed no induration post PPD injection and the rest were shows reading zone between 5 to 9 mm. The result shows that among the 39 positive participants 23 (58.97%) were male while only 16 (41.03%) were female. The mean of zone reading among the positive participants is higher in male 13.96 ± 3.29 than female 13.81 ± 2.22. The study concluded that more than half of the participants were negative for tuberculin test and this may be interpreted by either the vaccine was invalid or their cell-mediated immunity against TB is reduced. The discrepancy in the zone reading means between male and female may be related to some physiological difference. Further studies with more sample size and by using a more advanced technique (IFNγ) should be done to clarify the results.","PeriodicalId":74235,"journal":{"name":"Mycobacterial diseases : tuberculosis & leprosy","volume":"08 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70585820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antigens for DNA Vaccines Against Tuberculosis 抗结核DNA疫苗抗原

Mycobacterial diseases : tuberculosis & leprosy Pub Date : 2018-01-01 DOI: 10.4172/2161-1068.1000255

A. Mustafa

引用次数: 0

Intravesical BCG Therapy and Side Effects-Case Reports and a Review of Literature 膀胱内卡介苗治疗及副作用:病例报告及文献回顾

Mycobacterial diseases : tuberculosis & leprosy Pub Date : 2018-01-01 DOI: 10.4172/2161-1068.1000270

S. Vasudeva, A. Baffoe-Bonnie

引用次数: 4