Molecular biomedicine最新文献_第9页

Biomaterials barcoding: a high-throughput breakthrough. 生物材料条形码：高通量突破。

IF 4

Molecular biomedicine Pub Date : 2024-01-02 DOI: 10.1186/s43556-023-00163-x

Masoud Mozafari

引用次数: 0

Correction: Activation and induction of antigen-specific T follicular helper cells play a critical role in recombinant SARS-CoV-2 RBD vaccine-induced humoral responses. 更正：抗原特异性 T 滤泡辅助细胞的激活和诱导在重组 SARS-CoV-2 RBD 疫苗诱导的体液反应中起着关键作用。

IF 4

Molecular biomedicine Pub Date : 2023-12-18 DOI: 10.1186/s43556-023-00152-0

Songhao Yang, Liangwei Duan, Chan Wang, Cuiying Zhang, Siyu Hou, Hao Wang, Jiahui Song, Tingting Zhang, Zihua Li, Mingxia Wang, Jing Tang, Qianqian Zheng, Hui Wang, Qi Wang, Wei Zhao

引用次数: 0

Biofilm formation: mechanistic insights and therapeutic targets. 生物膜的形成：机理认识和治疗目标。

IF 6.3

Molecular biomedicine Pub Date : 2023-12-15 DOI: 10.1186/s43556-023-00164-w

Xinyu Wang, Ming Liu, Chuanjiang Yu, Jing Li, Xikun Zhou

{"title":"Biofilm formation: mechanistic insights and therapeutic targets.","authors":"Xinyu Wang, Ming Liu, Chuanjiang Yu, Jing Li, Xikun Zhou","doi":"10.1186/s43556-023-00164-w","DOIUrl":"10.1186/s43556-023-00164-w","url":null,"abstract":"Biofilms are complex multicellular communities formed by bacteria, and their extracellular polymeric substances are observed as surface-attached or non-surface-attached aggregates. Many types of bacterial species found in living hosts or environments can form biofilms. These include pathogenic bacteria such as Pseudomonas, which can act as persistent infectious hosts and are responsible for a wide range of chronic diseases as well as the emergence of antibiotic resistance, thereby making them difficult to eliminate. Pseudomonas aeruginosa has emerged as a model organism for studying biofilm formation. In addition, other Pseudomonas utilize biofilm formation in plant colonization and environmental persistence. Biofilms are effective in aiding bacterial colonization, enhancing bacterial resistance to antimicrobial substances and host immune responses, and facilitating cell‒cell signalling exchanges between community bacteria. The lack of antibiotics targeting biofilms in the drug discovery process indicates the need to design new biofilm inhibitors as antimicrobial drugs using various strategies and targeting different stages of biofilm formation. Growing strategies that have been developed to combat biofilm formation include targeting bacterial enzymes, as well as those involved in the quorum sensing and adhesion pathways. In this review, with Pseudomonas as the primary subject of study, we review and discuss the mechanisms of bacterial biofilm formation and current therapeutic approaches, emphasizing the clinical issues associated with biofilm infections and focusing on current and emerging antibiotic biofilm strategies.","PeriodicalId":74218,"journal":{"name":"Molecular biomedicine","volume":"4 1","pages":"49"},"PeriodicalIF":6.3,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10721784/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138812988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Delivery of nucleic acids using nanomaterials. 利用纳米材料输送核酸。

IF 4

Molecular biomedicine Pub Date : 2023-12-14 DOI: 10.1186/s43556-023-00160-0

Yuyang Qin, Liyuan Ou, Lili Zha, Yue Zeng, Ling Li

{"title":"Delivery of nucleic acids using nanomaterials.","authors":"Yuyang Qin, Liyuan Ou, Lili Zha, Yue Zeng, Ling Li","doi":"10.1186/s43556-023-00160-0","DOIUrl":"https://doi.org/10.1186/s43556-023-00160-0","url":null,"abstract":"The increasing number of approved nucleic acid therapeutics demonstrates the potential for the prevention and treatment of a broad spectrum of diseases. This trend underscores the significant impact and promise of nucleic acid-based treatments in the field of medicine. Nevertheless, employing nucleic acids as therapeutics is challenging due to their susceptibility to degradation by nucleases and their unfavorable physicochemical characteristics that hinder delivery into cells. Appropriate vectors play a pivotal role in improving nucleic acid stability and delivering nucleic acids into specific cells. The maturation of delivery systems has led to breakthroughs in the development of therapeutics based on nucleic acids such as DNA, siRNA, and mRNA. Non-viral vectors have gained prominence among the myriad of nanomaterials due to low immunogenicity, ease of manufacturing, and simplicity of cost-effective, large-scale production. Here, we provide an overview of the recent advancements in nanomaterials for nucleic acid delivery. Specifically, we give a detailed introduction to the characteristics of polymers, lipids, and polymer-lipid hybrids, and provide comprehensive descriptions of their applications in nucleic acid delivery. Also, biological barriers, administration routes, and strategies for organ-selective delivery of nucleic acids are discussed. In summary, this review offers insights into the rational design of next-generation delivery vectors for nucleic acid delivery.","PeriodicalId":74218,"journal":{"name":"Molecular biomedicine","volume":"4 1","pages":"48"},"PeriodicalIF":4.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10719232/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138812991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure, function and drug discovery of GPCR signaling. GPCR信号的结构、功能及药物发现。

IF 4

Molecular biomedicine Pub Date : 2023-12-04 DOI: 10.1186/s43556-023-00156-w

Lin Cheng, Fan Xia, Ziyan Li, Chenglong Shen, Zhiqian Yang, Hanlin Hou, Suyue Sun, Yuying Feng, Xihao Yong, Xiaowen Tian, Hongxi Qin, Wei Yan, Zhenhua Shao

{"title":"Structure, function and drug discovery of GPCR signaling.","authors":"Lin Cheng, Fan Xia, Ziyan Li, Chenglong Shen, Zhiqian Yang, Hanlin Hou, Suyue Sun, Yuying Feng, Xihao Yong, Xiaowen Tian, Hongxi Qin, Wei Yan, Zhenhua Shao","doi":"10.1186/s43556-023-00156-w","DOIUrl":"10.1186/s43556-023-00156-w","url":null,"abstract":"G protein-coupled receptors (GPCRs) are versatile and vital proteins involved in a wide array of physiological processes and responses, such as sensory perception (e.g., vision, taste, and smell), immune response, hormone regulation, and neurotransmission. Their diverse and essential roles in the body make them a significant focus for pharmaceutical research and drug development. Currently, approximately 35% of marketed drugs directly target GPCRs, underscoring their prominence as therapeutic targets. Recent advances in structural biology have substantially deepened our understanding of GPCR activation mechanisms and interactions with G-protein and arrestin signaling pathways. This review offers an in-depth exploration of both traditional and recent methods in GPCR structure analysis. It presents structure-based insights into ligand recognition and receptor activation mechanisms and delves deeper into the mechanisms of canonical and noncanonical signaling pathways downstream of GPCRs. Furthermore, it highlights recent advancements in GPCR-related drug discovery and development. Particular emphasis is placed on GPCR selective drugs, allosteric and biased signaling, polyphamarcology, and antibody drugs. Our goal is to provide researchers with a thorough and updated understanding of GPCR structure determination, signaling pathway investigation, and drug development. This foundation aims to propel forward-thinking therapeutic approaches that target GPCRs, drawing upon the latest insights into GPCR ligand selectivity, activation, and biased signaling mechanisms.","PeriodicalId":74218,"journal":{"name":"Molecular biomedicine","volume":"4 1","pages":"46"},"PeriodicalIF":4.0,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10695916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138479746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apolipoprotein E (ApoE) orchestrates adipose tissue inflammation and metabolic disorders through NLRP3 inflammasome 载脂蛋白 E（ApoE）通过 NLRP3 炎症小体协调脂肪组织炎症和代谢紊乱

IF 4

Molecular biomedicine Pub Date : 2023-12-01 DOI: 10.1186/s43556-023-00158-8

Yulin Zhang, Ziwei Cheng, Liyu Hong, Jia Liu, Xinyue Ma, Wenjing Wang, Ran Pan, Wenjie Lu, Qichao Luo, Shan Gao, Qin Kong

引用次数: 0

Mechanisms by which the intestinal microbiota affects gastrointestinal tumours and therapeutic effects. 肠道微生物群影响胃肠道肿瘤和治疗效果的机制。

IF 4

Molecular biomedicine Pub Date : 2023-11-30 DOI: 10.1186/s43556-023-00157-9

Jikai He, Haijun Li, Jiaqi Jia, Yang Liu, Ning Zhang, Rumeng Wang, Wenhao Qu, Yanqi Liu, Lizhou Jia

{"title":"Mechanisms by which the intestinal microbiota affects gastrointestinal tumours and therapeutic effects.","authors":"Jikai He, Haijun Li, Jiaqi Jia, Yang Liu, Ning Zhang, Rumeng Wang, Wenhao Qu, Yanqi Liu, Lizhou Jia","doi":"10.1186/s43556-023-00157-9","DOIUrl":"10.1186/s43556-023-00157-9","url":null,"abstract":"The intestinal microbiota is considered to be a forgotten organ in human health and disease. It maintains intestinal homeostasis through various complex mechanisms. A significant body of research has demonstrated notable differences in the gut microbiota of patients with gastrointestinal tumours compared to healthy individuals. Furthermore, the dysregulation of gut microbiota, metabolites produced by gut bacteria, and related signal pathways can partially explain the mechanisms underlying the occurrence and development of gastrointestinal tumours. Therefore, this article summarizes the latest research progress on the gut microbiota and gastrointestinal tumours. Firstly, we provide an overview of the composition and function of the intestinal microbiota and discuss the mechanisms by which the intestinal flora directly or indirectly affects the occurrence and development of gastrointestinal tumours by regulating the immune system, producing bacterial toxins, secreting metabolites. Secondly, we present a detailed analysis of the differences of intestinal microbiota and its pathogenic mechanisms in colorectal cancer, gastric cancer, hepatocellular carcinoma, etc. Lastly, in terms of treatment strategies, we discuss the effects of the intestinal microbiota on the efficacy and toxic side effects of chemotherapy and immunotherapy and address the role of probiotics, prebiotics, FMT and antibiotic in the treatment of gastrointestinal tumours. In summary, this article provides a comprehensive review of the pathogenic mechanisms of and treatment strategies pertaining to the intestinal microbiota in patients with gastrointestinal tumours. And provide a more comprehensive and precise scientific basis for the development of microbiota-based treatments for gastrointestinal tumours and the prevention of such tumours.","PeriodicalId":74218,"journal":{"name":"Molecular biomedicine","volume":"4 1","pages":"45"},"PeriodicalIF":4.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10689341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138464900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Osteopontin enhances the effect of treadmill training and promotes functional recovery after spinal cord injury. 骨桥蛋白增强跑步机训练的效果，促进脊髓损伤后的功能恢复。

IF 4

Molecular biomedicine Pub Date : 2023-11-28 DOI: 10.1186/s43556-023-00154-y

Yunhang Wang, Hong Su, Juan Zhong, Zuxiong Zhan, Qin Zhao, Yuan Liu, Sen Li, Haiyan Wang, Ce Yang, Lehua Yu, Botao Tan, Ying Yin

{"title":"Osteopontin enhances the effect of treadmill training and promotes functional recovery after spinal cord injury.","authors":"Yunhang Wang, Hong Su, Juan Zhong, Zuxiong Zhan, Qin Zhao, Yuan Liu, Sen Li, Haiyan Wang, Ce Yang, Lehua Yu, Botao Tan, Ying Yin","doi":"10.1186/s43556-023-00154-y","DOIUrl":"10.1186/s43556-023-00154-y","url":null,"abstract":"In this study, we examined the combined impact of osteopontin (OPN) and treadmill training on mice with spinal cord injury (SCI). OPN was overexpressed by injecting AAV9-SPP1-GFP into the sensorimotor cortex, followed by a left incomplete C5 crush injury two weeks later. Mice (Ex or Ex + OPN group) were trained at 50% maximum running speed for 8 weeks. To analyze the effects, we used biotinylated dextran amine (BDA) for tracing the corticospinal tract (CST) and performed Western blotting and immunohistochemical methods to assess the activation of the mammalian target of rapamycin (mTOR). We also examined axonal regeneration and conducted behavioral tests to measure functional recovery. The results demonstrated that treadmill training promoted the expression of neurotrophic factors such as brain-derived neurotrophic factor (BNDF) and insulin-like growth factor I (IGF-1) and activated mTOR signaling. OPN amplified the effect of treadmill training on activating mTOR signaling indicated by upregulated phosphorylation of ribosomal protein S6 kinase (S6). The combination of OPN and exercise further promoted functional recovery and facilitated limited CST axonal regeneration which did not occur with treadmill training and OPN treatment alone. These findings indicate that OPN enhances the effects of treadmill training in the treatment of SCI and offer new therapeutic insights for spinal cord injury.","PeriodicalId":74218,"journal":{"name":"Molecular biomedicine","volume":"4 1","pages":"44"},"PeriodicalIF":4.0,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10684450/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138447428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MSCs overexpressing GDNF restores brain structure and neurological function in rats with intracerebral hemorrhage. 过表达GDNF的间充质干细胞可恢复脑出血大鼠的脑结构和神经功能。

IF 4

Molecular biomedicine Pub Date : 2023-11-27 DOI: 10.1186/s43556-023-00159-7

Xiaoqian Jiang, Ling Zhou, Zihuan Sun, Bingqing Xie, Heng Lin, Xiaoqing Gao, Li Deng, Chaoxian Yang

{"title":"MSCs overexpressing GDNF restores brain structure and neurological function in rats with intracerebral hemorrhage.","authors":"Xiaoqian Jiang, Ling Zhou, Zihuan Sun, Bingqing Xie, Heng Lin, Xiaoqing Gao, Li Deng, Chaoxian Yang","doi":"10.1186/s43556-023-00159-7","DOIUrl":"10.1186/s43556-023-00159-7","url":null,"abstract":"Mesenchymal stem cells (MSCs) have been applied in transplantation to treat intracerebral hemorrhage (ICH) but with limited efficacy. Accumulated evidence has shown that glial cell-derived neurotrophic factor (GDNF) plays a crucial part in neuronal protection and functional recovery of the brain after ICH; however, GDNF has difficulty crossing the blood-brain barrier, which limits its application. In this study, we investigated the influences of MSCs overexpressing GDNF (MSCs/GDNF) on the brain structure as well as gait of rats after ICH and explored the possible mechanisms. We found that cell transplantation could reverse the neurological dysfunction and brain damage caused by ICH to a certain extent, and MSCs/GDNF transplantation was superior to MSCs transplantation. Moreover, Transplantation of MSCs overexpressing GDNF effectively reduced the volume of bleeding foci and increased the level of glucose uptake in rats with ICH, which could be related to improving mitochondrial quality. Furthermore, GDNF produced by transplanted MSCs/GDNF further inhibited neuroinflammation, improved mitochondrial quality and function, promoted angiogenesis and the survival of neurons and oligodendrocytes, and enhanced synaptic plasticity in ICH rats when compared with simple MSC transplantation. Overall, our data indicate that GDNF overexpression heightens the curative effect of MSC implantation in treating rats following ICH.","PeriodicalId":74218,"journal":{"name":"Molecular biomedicine","volume":"4 1","pages":"43"},"PeriodicalIF":4.0,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10678901/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138441793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aloperine targets lysosomes to inhibit late autophagy and induces cell death through apoptosis and paraptosis in glioblastoma. 丙operine在胶质母细胞瘤中靶向溶酶体抑制晚期自噬，通过细胞凋亡和细胞凋亡诱导细胞死亡。

IF 4

Molecular biomedicine Pub Date : 2023-11-17 DOI: 10.1186/s43556-023-00155-x

Ting Tang, Hui Liang, Wuting Wei, Yanling Han, Liang Cao, Zixiang Cong, Shiqiao Luo, Handong Wang, Meng-Liang Zhou

{"title":"Aloperine targets lysosomes to inhibit late autophagy and induces cell death through apoptosis and paraptosis in glioblastoma.","authors":"Ting Tang, Hui Liang, Wuting Wei, Yanling Han, Liang Cao, Zixiang Cong, Shiqiao Luo, Handong Wang, Meng-Liang Zhou","doi":"10.1186/s43556-023-00155-x","DOIUrl":"10.1186/s43556-023-00155-x","url":null,"abstract":"Glioblastoma (GBM) is an aggressive intracranial tumour, and current chemotherapy regimens have limited efficacy. Aloperine (ALO), a natural alkaline compound, has shown potential as an antitumor agent. However, the effect of ALO against GBM remains unclear. This study aimed to investigate the function of ALO in treating GBM. U87, A172, and GL261 cell lines were used for in vitro experiments, and GL261 was also used to establish in vivo models. The results showed that ALO inhibited the proliferation of GBM cells by cell cycle arrest and apoptosis. Furthermore, autophagy was found to play a critical role, suggested by observation of autophagosomes under the transmission electron microscopy. It was discovered for the first time that ALO targeted lysosomes directly in glioma cells, tested by fluo-rescence-labelled ALO and organelle-localizing probes. In addition, ALO inhibited late autophagy and induced paraptosis in GBM, verified by classical gene expression changes in qPCR and western blotting. Also, ALO inhibited tumour growth and acted synergistically with temozolomide in intracranial glioma mice models in vivo. Our findings suggest that ALO targets lysosomes to inhibit late autophagy in GBM, inducing cell cycle arrest, paraptosis, and apoptosis. ALO may therefore be a promising therapeutic agent for the treatment of GBM.","PeriodicalId":74218,"journal":{"name":"Molecular biomedicine","volume":"4 1","pages":"42"},"PeriodicalIF":4.0,"publicationDate":"2023-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10656413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136400659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0