Advances in Pharmacological Sciences最新文献_第10页

Targeting AGEs Signaling Ameliorates Central Nervous System Diabetic Complications in Rats. 靶向AGEs信号通路改善大鼠中枢神经系统糖尿病并发症。

Advances in Pharmacological Sciences Pub Date : 2015-01-01 Epub Date: 2015-09-29 DOI: 10.1155/2015/346259

Mohamed Naguib Zakaria, Hany M El-Bassossy, Waleed Barakat

{"title":"Targeting AGEs Signaling Ameliorates Central Nervous System Diabetic Complications in Rats.","authors":"Mohamed Naguib Zakaria, Hany M El-Bassossy, Waleed Barakat","doi":"10.1155/2015/346259","DOIUrl":"https://doi.org/10.1155/2015/346259","url":null,"abstract":"Diabetes is a chronic endocrine disorder associated with several complications as hypertension, advanced brain aging, and cognitive decline. Accumulation of advanced glycation end products (AGEs) is an important mechanism that mediates diabetic complications. Upon binding to their receptor (RAGE), AGEs mediate oxidative stress and/or cause cross-linking with proteins in blood vessels and brain tissues. The current investigation was designed to investigate the effect of agents that decrease AGEs signaling, perindopril which increases soluble RAGE (sRAGE) and alagebrium which cleaves AGEs cross-links, compared to the standard antidiabetic drug, gliclazide, on the vascular and central nervous system (CNS) complications in STZ-induced (50 mg/kg, IP) diabetes in rats. Perindopril ameliorated the elevation in blood pressure seen in diabetic animals. In addition, both perindopril and alagebrium significantly inhibited memory decline (performance in the Y-maze), neuronal degeneration (Fluoro-Jade staining), AGEs accumulation in serum and brain, and brain oxidative stress (level of reduced glutathione and activities of catalase and malondialdehyde). These results suggest that blockade of AGEs signaling after diabetes induction in rats is effective in reducing diabetic CNS complications. ","PeriodicalId":7389,"journal":{"name":"Advances in Pharmacological Sciences","volume":"2015 ","pages":"346259"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/346259","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34109123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Validated HPTLC Method for Quantification of Luteolin and Apigenin in Premna mucronata Roxb., Verbenaceae. 高效液相色谱法测定牛膝草中木犀草素和芹菜素的含量。马鞭草科。

Advances in Pharmacological Sciences Pub Date : 2015-01-01 Epub Date: 2015-09-03 DOI: 10.1155/2015/682365

Nayan G Patel, Kalpana G Patel, Kirti V Patel, Tejal R Gandhi

{"title":"Validated HPTLC Method for Quantification of Luteolin and Apigenin in Premna mucronata Roxb., Verbenaceae.","authors":"Nayan G Patel, Kalpana G Patel, Kirti V Patel, Tejal R Gandhi","doi":"10.1155/2015/682365","DOIUrl":"https://doi.org/10.1155/2015/682365","url":null,"abstract":"A simple, rapid, and precise high-performance thin-layer chromatographic method was developed for quantitative estimation of luteolin and apigenin in Premna mucronata Roxb., family Verbenaceae. Separation was performed on silica gel 60 F254 HPTLC plates using toluene : ethyl acetate : formic acid (6 : 4 : 0.3) as mobile phase for elution of markers from extract. The determination was carried out in fluorescence mode using densitometric absorbance-reflection mode at 366 nm for both luteolin and apigenin. The methanolic extract of Premna mucronata was found to contain 10.2 mg/g % luteolin and 0.165 mg/g % of apigenin. The method was validated in terms of linearity, LOD and LOQ, accuracy, precision, and specificity. The calibration curve was found to be linear between 200 and 1000 ng/band for luteolin and 50 and 250 ng/band for apigenin. For luteolin and apigenin, the limit of detection was found to be 42.6 ng/band and 7.97 ng/band while the limit of quantitation was found to be 129.08 ng/band and 24.155 ng/band, respectively. This developed validated method is capable of quantifying and resolving luteolin and apigenin and can be applicable for routine analysis of extract and plant as a whole. ","PeriodicalId":7389,"journal":{"name":"Advances in Pharmacological Sciences","volume":"2015 ","pages":"682365"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/682365","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34217035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Spirulina platensis Lacks Antitumor Effect against Solid Ehrlich Carcinoma in Female Mice. 螺旋藻对雌性小鼠实体埃利希癌缺乏抗肿瘤作用。

Advances in Pharmacological Sciences Pub Date : 2015-01-01 Epub Date: 2015-08-20 DOI: 10.1155/2015/132873

Waleed Barakat, Shimaa M Elshazly, Amr A A Mahmoud

引用次数: 27

Evaluation of Antidiarrheal Activity of Methanolic Extract of Maranta arundinacea Linn. Leaves. 评价马兰塔蒿属植物甲醇提取物的止泻活性叶片。

Advances in Pharmacological Sciences Pub Date : 2015-01-01 Epub Date: 2015-08-06 DOI: 10.1155/2015/257057

Md Khalilur Rahman, Md Ashraf Uddin Chowdhury, Mohammed Taufiqual Islam, Md Anisuzzaman Chowdhury, Muhammad Erfan Uddin, Chandra Datta Sumi

{"title":"Evaluation of Antidiarrheal Activity of Methanolic Extract of Maranta arundinacea Linn. Leaves.","authors":"Md Khalilur Rahman, Md Ashraf Uddin Chowdhury, Mohammed Taufiqual Islam, Md Anisuzzaman Chowdhury, Muhammad Erfan Uddin, Chandra Datta Sumi","doi":"10.1155/2015/257057","DOIUrl":"10.1155/2015/257057","url":null,"abstract":"Diarrhea is one of the most common causes for thousands of deaths every year. Therefore, identification of new source of antidiarrheal drugs becomes one of the most prominent focuses in modern research. Our aim was to investigate the antidiarrheal and cytotoxic activities of methanolic extract of Maranta arundinacea linn. (MEMA) leaves in rats and brine shrimp, respectively. Antidiarrheal effect was evaluated by using castor oil-induced diarrhea, enteropooling, and gastrointestinal motility tests at 200 mg/kg and 400 mg/kg body weight in rats where the cytotoxic activity was justified using brine shrimp lethality bioassay at different concentrations of MEMA. The extract showed considerable antidiarrheal effect by inhibiting 42.67% and 57.75% of diarrheal episode at the doses of 200 and 400 mg/kg, respectively. MEMA also significantly (p < 0.01) reduced the castor oil-induced intestinal volume (2.14 ± 0.16 to 1.61 ± 0.12 mL) in enteropooling test as well as intestinal transit (33.00 to 43.36%) in GI motility test, compared to their respective control. These observed effects are comparable to that of standard drug loperamide (5 mg/kg). On the other hand, in brine shrimp lethality test after 24 h, surviving brine shrimp larvae were counted and LD50 was assessed. Result showed that MEMA was potent against brine shrimp with LD50 value of 420 µg/mL. So the highest dose of 400 µg/mL of MEMA was not toxic to mice. So these results indicate that bioactive compounds are present in methanolic extract of Maranta arundinacea leaves including significant antidiarrheal activity and could be accounted for pharmacological effects. ","PeriodicalId":7389,"journal":{"name":"Advances in Pharmacological Sciences","volume":"2015 ","pages":"257057"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4543376/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34050675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melatonin and Ischemic Stroke: Mechanistic Roles and Action. 褪黑激素与缺血性中风：机制作用和作用。

Advances in Pharmacological Sciences Pub Date : 2015-01-01 Epub Date: 2015-09-07 DOI: 10.1155/2015/384750

Syed Suhail Andrabi, Suhel Parvez, Heena Tabassum

引用次数: 0

Antisecretory Action of the Extract of the Aerial Parts of Eremomastax speciosa (Acanthaceae) Occurs through Antihistaminic and Anticholinergic Pathways. 刺科毛蕨(Eremomastax speciosa)地上部提取物的抗分泌作用通过抗组胺和抗胆碱能途径发生。

Advances in Pharmacological Sciences Pub Date : 2014-01-01 Epub Date: 2014-02-20 DOI: 10.1155/2014/323470

Amang André Perfusion, Paul V Tan, Nkwengoua Ernestine, Nyasse Barthélemy

{"title":"Antisecretory Action of the Extract of the Aerial Parts of Eremomastax speciosa (Acanthaceae) Occurs through Antihistaminic and Anticholinergic Pathways.","authors":"Amang André Perfusion, Paul V Tan, Nkwengoua Ernestine, Nyasse Barthélemy","doi":"10.1155/2014/323470","DOIUrl":"https://doi.org/10.1155/2014/323470","url":null,"abstract":"Objective. The objective of this study was to find out the possible antiulcer mechanism of action of Eremomastax speciosa. Method. Carbachol- and histamine-induced hypersecretion, associated with the pylorus ligation technique, were used in rats. Gastric mucosal ulceration, mucus production, pH, gastric volume, and acidity were measured. Results. Histamine and carbachol raised gastric acidity to 86.50 and 84.80 mEq/L, respectively, in the control rats, and the extracts (200 mg/kg) reduced gastric acidity to 34.60 and 39.00 mEq/L, respectively. Intraduodenal aqueous extract (400 mg/kg) in histamine- and carbachol-treated rats produced significant (P < 0.001) decreases in acid secretion to 28.50 and 28.80 mEq/L, respectively, and 100 percent inhibition of gastric ulceration. Augmented histamine-induced gastric acid secretion (90.20 mEq/L) was significantly reduced to 52.60 and 27.50 mEq/L by the 200 and 400 mg/kg doses of the aqueous extract, respectively. The extract significantly reduced (P < 0.001) the volume of gastric secretion and significantly increased mucus production. The ulcer inhibition potential of the extract significantly dropped to 25-44% (oral extract) and to 29-37% (duodenal extract) in carbachol/indomethacin-treated rats. Conclusion. The aqueous extract of E. speciosa has both cytoprotective and antisecretory effects. The antisecretory effect may involve a mechanism common to both cholinergic and histaminergic pathways. ","PeriodicalId":7389,"journal":{"name":"Advances in Pharmacological Sciences","volume":"2014 ","pages":"323470"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/323470","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32231386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Evaluation of the Potential Nephroprotective and Antimicrobial Effect of Camellia sinensis Leaves versus Hibiscus sabdariffa (In Vivo and In Vitro Studies). 茶树叶与芙蓉叶潜在的肾保护和抗菌作用的体内和体外研究

Advances in Pharmacological Sciences Pub Date : 2014-01-01 Epub Date: 2014-05-14 DOI: 10.1155/2014/389834

Doa'a Anwar Ibrahim, Rowida Noman Albadani

{"title":"Evaluation of the Potential Nephroprotective and Antimicrobial Effect of Camellia sinensis Leaves versus Hibiscus sabdariffa (In Vivo and In Vitro Studies).","authors":"Doa'a Anwar Ibrahim, Rowida Noman Albadani","doi":"10.1155/2014/389834","DOIUrl":"https://doi.org/10.1155/2014/389834","url":null,"abstract":"Green tea and hibiscus are widely consumed as traditional beverages in Yemen and some regional countries. They are relatively cheap and the belief is that they improve health state and cure many diseases. The aim of this study was to evaluate the potential protective and antibacterial activity of these two famous plants in vitro through measuring their antibacterial activity and in vivo through measuring nonenzymatic kidney markers dysfunction after induction of nephrotoxicity by gentamicin. Gram positive bacteria like MRSA (methicillin resistant Staphylococcus aureus) were isolated from hospitalized patients' different sources (pus and wound) and Gram negative bacteria including E. coli and P. aeruginosa were used in vitro study. In addition, the efficacy of these plants was assessed in vivo through measuring nonenzymatic kidney markers including S. creatinine and S. urea. Green tea was shown antimicrobial activity against MRSA with inhibition zone 19.67 ± 0.33 mm and MIC 1.25 ± 0.00 mg/mL compared with standard reference (vancomycin) 18.00 ± 0.00 mg/mL. Hibiscus did not exhibit a similar effect. Both Hibiscus- and green tea-treated groups had nephroprotective effects as they reduced the elevation in nonenzymatic kidney markers. We conclude that green tea has dual effects: antimicrobial and nephroprotective. ","PeriodicalId":7389,"journal":{"name":"Advances in Pharmacological Sciences","volume":"2014 ","pages":"389834"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/389834","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32441983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Effects of Methanol Extract of Breadfruit (Artocarpus altilis) on Atherogenic Indices and Redox Status of Cellular System of Hypercholesterolemic Male Rats. 面包果甲醇提取物对高胆固醇血症雄性大鼠动脉粥样硬化指标及细胞系统氧化还原状态的影响。

Advances in Pharmacological Sciences Pub Date : 2014-01-01 Epub Date: 2014-01-30 DOI: 10.1155/2014/605425

Oluwatosin Adekunle Adaramoye, Olubukola Oyebimpe Akanni

{"title":"Effects of Methanol Extract of Breadfruit (Artocarpus altilis) on Atherogenic Indices and Redox Status of Cellular System of Hypercholesterolemic Male Rats.","authors":"Oluwatosin Adekunle Adaramoye, Olubukola Oyebimpe Akanni","doi":"10.1155/2014/605425","DOIUrl":"https://doi.org/10.1155/2014/605425","url":null,"abstract":"We investigated the effects of methanol extract of Artocarpus altilis (AA) on atherogenic indices and redox status of cellular system of rats fed with dietary cholesterol while Questran (QUE) served as standard. Biochemical indices such as total cholesterol (TC), triglycerides (TG), low- and high-density lipoproteins-cholesterol (LDL-C and HDL-C), aspartate and alanine aminotransferases (AST and ALT), lactate dehydrogenase (LDH), reduced glutathione, glutathione-s-transferase, glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD), and lipid peroxidation (LPO) were assessed. Hypercholesterolemic (HC) rats had significantly increased relative weight of liver and heart. Dietary cholesterol caused a significant increase (P < 0.05) in the levels of serum, hepatic, and cardiac TC by 110%, 70%, and 85%, LDL-C by 79%, 82%, and 176%, and TG by 68%, 96%, and 62%, respectively. Treatment with AA significantly reduced the relative weight of the organs and lipid parameters. There were beneficial increases in serum and cardiac HDL-C levels in HC rats treated with AA. In HC rats, serum LDH, ALT, and AST activities and levels of LPO were increased, whereas hepatic and cardiac SOD, CAT, and GPx were reduced. All biochemical and histological alterations were ameliorated upon treatment with AA. Extract of AA had protective effects against dietary cholesterol-induced hypercholesterolemia. ","PeriodicalId":7389,"journal":{"name":"Advances in Pharmacological Sciences","volume":"2014 ","pages":"605425"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/605425","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32172444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Influence of flunixin on the disposition kinetic of cefepime in goats. 氟尼辛对山羊对头孢吡肟处置动力学的影响。

Advances in Pharmacological Sciences Pub Date : 2014-01-01 Epub Date: 2014-05-05 DOI: 10.1155/2014/471517

Mohamed El-Hewaity

{"title":"Influence of flunixin on the disposition kinetic of cefepime in goats.","authors":"Mohamed El-Hewaity","doi":"10.1155/2014/471517","DOIUrl":"https://doi.org/10.1155/2014/471517","url":null,"abstract":"The pharmacokinetic profile of cefepime (10 mg/kg b.w.) was studied following intravenous and intramuscular administration of cefepime alone and coadministered with flunixin (2.2 mg/kg b.w.) in goats. Cefepime concentrations in serum were determined by microbiological assay technique using Escherichia coli (MTCC 443) as test organism. Following intravenous injection of cefepime alone and in combination with flunixin, there are no significant changes in the pharmacokinetic parameters. Following intramuscular injection of cefepime alone and in combination with flunixin, the maximum serum concentration was significantly increased in flunixin coadministered group compared with cefepime alone. However, no significant changes were reported in other pharmacokinetic parameters. The result of in vitro protein binding study indicated that 15.62% of cefepime was bound to goat's serum protein. The mean bioavailability was 92.66% and 95.27% in cefepime alone and coadministered with flunixin, respectively. The results generated from the present study suggest that cefepime may be coadministered with flunixin without change in dose regimen. Cefepime may be given intramuscularly at 12 h intervals to combat susceptible bacterial infections. ","PeriodicalId":7389,"journal":{"name":"Advances in Pharmacological Sciences","volume":"2014 ","pages":"471517"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/471517","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32387154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Teratogenic effects of coadministration of fluoxetine and olanzapine on rat fetuses. 氟西汀与奥氮平合用对大鼠胎儿的致畸作用。

Advances in Pharmacological Sciences Pub Date : 2014-01-01 Epub Date: 2014-01-15 DOI: 10.1155/2014/132034

Azam Bakhtiarian, Nasrin Takzare, Mehdi Sheykhi, Narges Sistany, Farahnaz Jazaeri, Mario Giorgi, Vahid Nikoui

{"title":"Teratogenic effects of coadministration of fluoxetine and olanzapine on rat fetuses.","authors":"Azam Bakhtiarian, Nasrin Takzare, Mehdi Sheykhi, Narges Sistany, Farahnaz Jazaeri, Mario Giorgi, Vahid Nikoui","doi":"10.1155/2014/132034","DOIUrl":"https://doi.org/10.1155/2014/132034","url":null,"abstract":"Objective. Depression during pregnancy is a relatively common problem. Since little is known about the teratogenic effects of concomitant administration of fluoxetine and olanzapine during the organogenesis period, the aim of the present study was to evaluate the teratogenic effects of coadministration of fluoxetine and olanzapine on rat fetuses. Method. Forty-two pregnant rats were divided into seven groups, randomly. The first group received 0.5 mL of normal saline as the control. The second and third groups received fluoxetine at doses of 9 mg/kg and 18 mg/kg, respectively. Olanzapine was injected at 3 mg/kg and 6 mg/kg to the fourth and fifth groups, respectively. The sixth group received 9 mg/kg fluoxetine and 3 mg/kg olanzapine. Finally, the seventh group was administrated with fluoxetine and olanzapine at 18 mg/kg and 6 mg/kg, respectively. Drugs were injected intraperitoneally between day eight and day 15 of the pregnancy. On the 17th day of pregnancy, the fetuses were removed and micro-/macroscopically studied. Results. Fetuses of rats receiving high doses of these drugs showed a significant rate of cleft palate development, premature eyelid opening and torsion anomalies, compared to the control group (P ≤ 0.01). It is concluded that these drugs can lead to teratogenicity, so their concomitant use during pregnancy should be avoided, or if necessary their doses must be decreased. ","PeriodicalId":7389,"journal":{"name":"Advances in Pharmacological Sciences","volume":"2014 ","pages":"132034"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/132034","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32115280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2