Journal of molecular biochemistry最新文献

DNA Replication Control: Liquid-liquid Phase Separation Comes Into Play. DNA复制控制:液-液相分离开始发挥作用。

Journal of molecular biochemistry Pub Date : 2020-12-30

Patroula Nathanailidou, Stavros Taraviras, Zoi Lygerou

引用次数: 0

The Effect of the Pythagorean Self-Awareness Intervention on Psychological, Lifestyle and Cognitive Measures of a Community Sample. 毕达哥拉斯自我意识干预对社区样本心理、生活方式和认知测量的影响。

Journal of molecular biochemistry Pub Date : 2020-01-01 Epub Date: 2020-12-30

Eleni S Zigkiri, Nicolas C Nicolaides, Flora Bacopoulou, Dimitris Simos, Dimitrios Vlachakis, George P Chrousos, Christina Darviri

{"title":"The Effect of the Pythagorean Self-Awareness Intervention on Psychological, Lifestyle and Cognitive Measures of a Community Sample.","authors":"Eleni S Zigkiri, Nicolas C Nicolaides, Flora Bacopoulou, Dimitris Simos, Dimitrios Vlachakis, George P Chrousos, Christina Darviri","doi":"","DOIUrl":"","url":null,"abstract":"Background: The aim of this study was to evaluate the effect of a novel cognitive-behavioral stress management intervention, termed \"Pythagorean Self-Awareness Intervention\" (PSAI). Materials and Methods: The PSAI was applied to a community sample for eight weeks. Measurements included demographic characteristics, daily habits, sleep quality, fatigue, perceived stress levels and depressive symptoms. Cognitive function was measured by the Symbol Digits Modality Test (SDMT), the California Verbal Learning Test (CVLT) and the Brief Visuospatial Memory Test-Revised (BVMT-R).Results: The high-compliance group showed statistically significant differences in healthy lifestyle and personal control choices (HLPCQ; p=0.006), sleep quality (PSQI; p=0.007), self-regulation eating behavior (SREBQ; p=0.009), perceived stress (PSS; p=0.001) and depressive symptoms (p=0.003). Conclusions: Frequent practice of the PSAI had a positive effect on the psychological, lifestyle, and cognitive measures of the community sample. Larger studies are needed to establish the PSAI as an effective stress management method.","PeriodicalId":73836,"journal":{"name":"Journal of molecular biochemistry","volume":" ","pages":"32-40"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7842312/pdf/nihms-1661775.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25315990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reliability and Validity of the Newly Diagnosed Breast Cancer Stress Scale in the Greek Population. 希腊人群中新诊断乳腺癌应激量表的信度和效度。

Journal of molecular biochemistry Pub Date : 2020-01-01 Epub Date: 2020-12-30

Maria Charalampopoulou, Konstantinos Syrigos, Evaggelos Filopoulos, Vasileios Megalooikonomou, Dimitrios Vlachakis, George Chrousos, Christina Darviri

{"title":"Reliability and Validity of the Newly Diagnosed Breast Cancer Stress Scale in the Greek Population.","authors":"Maria Charalampopoulou, Konstantinos Syrigos, Evaggelos Filopoulos, Vasileios Megalooikonomou, Dimitrios Vlachakis, George Chrousos, Christina Darviri","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To examine the validity and the reliability of a novel measurement tool, the Newly Diagnosed Breast Cancer Stress Scale (NDBCSS) in the Greek population. The tool aimed to assess distress in patients recently diagnosed with breast cancer.Methods: We performed a principal component analysis (PCA) of the 17 items of the scale.Results: The PCA resulted in 4 factors: 1. Personal life, 2. Procedural issues, 3. Facing challenges and 4. Psychological load. All subscales showed satisfactory internal consistency and variance, relative to theoretical score ranges. Subscale scores and total score were significantly correlated with perceived stress and hospital anxiety and depression scale, implying good criterion validity. Associations with social, demographic and disease related information were also found.Conclusions: The NDBCSS resulted in acceptable reliability and good validity, and was considered as a valuable tool for health-care workers and oncologists to measure psychological distress in early stage of breast cancer.","PeriodicalId":73836,"journal":{"name":"Journal of molecular biochemistry","volume":"9 ","pages":"5-12"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7861504/pdf/nihms-1661772.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25341591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validation of the Stress in Children (SiC) Questionnaire in a Sample of Greek Pupils. 儿童压力(SiC)问卷在希腊小学生样本中的验证。

Journal of molecular biochemistry Pub Date : 2020-01-01 Epub Date: 2020-12-30

Chrysoula Emmanouil, Flora Bacopoulou, Dimitrios Vlachakis, George P Chrousos, Christina Darviri

{"title":"Validation of the Stress in Children (SiC) Questionnaire in a Sample of Greek Pupils.","authors":"Chrysoula Emmanouil, Flora Bacopoulou, Dimitrios Vlachakis, George P Chrousos, Christina Darviri","doi":"","DOIUrl":"","url":null,"abstract":"Aim: The purpose of this study was to validate a Greek version of the Stress in Children (SiC) Questionnaire and assess its psychometric qualities in a Greek pediatric sample.Materials and methods: Healthy children aged 8-12 years, attending schools of various areas of Attica, the largest region in Greece, were eligible to participate in the study. Data were collected from May 2016 until June 2017, anonymously, though three self-reported questionnaires, a simple demographic questionnaire, the SiC Questionnaire and the State and Trait Anxiety in Children Questionnaire.Results: Questionnaires were completed by 100 pupils (54 boys, 46 girls) aged (mean ± SD) 10.6 ± 1.26 years. A three-factor structure (\"Distress\", \"Presence of well-being\", and \"Presence of social support\") accounted for 41.33% of variance. Reliability Cronbach's alpha of \"Distress\", \"Presence of well-being\" and \"Presence of social support\" factors was equal to 0.75, 0.75 and 0.61, respectively. Regarding concurrent validity, positive correlations were found between \"Distress\" and both trait anxiety (rho=0.59, p<0.0001) and state anxiety (rho=0.61, p<0.0001). Correlations were also found between \"Presence of Wellbeing\" and age (rho=-0.24, p=0.019), state (rho=-0.36, p=0.006) and trait anxiety (rho=-0.45, p<0.0001).Conclusions: The Greek version of SiC Questionnaire demonstrated satisfactory psychometric properties (content validity, internal reliability) that support its use in Greek children.","PeriodicalId":73836,"journal":{"name":"Journal of molecular biochemistry","volume":" ","pages":"74-79"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7842352/pdf/nihms-1661776.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25315991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of the Pythagorean Self Awareness Intervention on Childhood Emotional Eating and Psychological Wellbeing: a Pragmatic Trial. 毕达哥拉斯自我意识干预对儿童情绪饮食和心理健康的影响:一项实用试验。

Journal of molecular biochemistry Pub Date : 2020-01-01 Epub Date: 2020-12-30

Despina S Kalogiratou, Flora Bacopoulou, Christina Kanaka-Gantenbein, Xanthi Tigani, Orsalia Gerakini, Dimitrios Vlachakis, George P Chrousos, Christina Darviri

{"title":"Effects of the Pythagorean Self Awareness Intervention on Childhood Emotional Eating and Psychological Wellbeing: a Pragmatic Trial.","authors":"Despina S Kalogiratou, Flora Bacopoulou, Christina Kanaka-Gantenbein, Xanthi Tigani, Orsalia Gerakini, Dimitrios Vlachakis, George P Chrousos, Christina Darviri","doi":"","DOIUrl":"","url":null,"abstract":"Background: Emotional eating is the tendency to regulate negative emotions (such as stress, depression and anxiety) through increased or unhealthy food intake. Emotion management, such as stress management could be an appropriate approach to prevent or control maladaptive eating behavior among children.Materials and methods: This is a controlled pragmatic trial, testing the effects of Pythagorean Self Awareness Intervention (PSAI), a cognitive stress management program in a primary school setting. The eligibility criterion was being a 4th grade active pupil. Participants were randomly assigned to either the intervention group (n = 23) or the control group (n = 22). Self-report measures were used for the evaluation of various variables at the beginning and the end of the 8-week monitoring period. Descriptive and inferential statistic methods were used for the statistical analysis.Results: At the end of the 8-week period pupils in the intervention group experienced statistically significant reduction in stress (SIC, p < 0.001), anxiety (STAIC-trait anxiety, p = 0.019, STAIC-state anxiety, p = 0.006), guilt (p < 0.001) and emotional eating (EES-C, p < 0.001) and an increase in Mediterranean diet quality (KIDMED, p = 0.001) and pride (p < 0.001). No statistically significant differences between the two groups were recorded regarding depression symptoms (CDI, p = 0.551) and shame (p = 0.120).Conclusions: PSAI had positive effects on a sample of primary school attendants' emotional eating and psychological state.","PeriodicalId":73836,"journal":{"name":"Journal of molecular biochemistry","volume":" ","pages":"13-21"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7842354/pdf/nihms-1661773.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25316513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An 8-week Stress Management Program in Information Technology Professionals and the Role of a New Cognitive Behavioral Method: a Pilot Randomized Controlled Trial. 一项为期8周的信息技术专业人员压力管理计划和一种新的认知行为方法的作用:一项随机对照试验。

Journal of molecular biochemistry Pub Date : 2020-01-01 Epub Date: 2020-12-30

Evangelia K Sioula, Xanthi Tigani, Artemios K Artemiadis, Dimitrios Vlachakis, George P Chrousos, Christina Darviri, Evangelos C Alexopoulos

{"title":"An 8-week Stress Management Program in Information Technology Professionals and the Role of a New Cognitive Behavioral Method: a Pilot Randomized Controlled Trial.","authors":"Evangelia K Sioula, Xanthi Tigani, Artemios K Artemiadis, Dimitrios Vlachakis, George P Chrousos, Christina Darviri, Evangelos C Alexopoulos","doi":"","DOIUrl":"","url":null,"abstract":"Aim: The aim of this study is to compare and evaluate the sort-term benefits of the effects of an 8-week stress management techniques in information technology professionals.Methods: In this parallel randomized controlled trial, participants were randomly assigned to either the stress management group (n=40; relaxation breathing, progressive muscle relaxation, guided imagery) or in the Pythagorean Self awareness group (n=41). Self-reported validated measures were used to evaluate perceived stress, health locus of control, anxiety and depression.Results: All groups were found with significantly better cognitive speed and verbal memory at the end of the follow-up. Taking into account the group by time interaction coefficients, PSAT was found significantly superior to standard SM with regards to depression, emotional intelligence, lifestyle and personal control and verbal memory suggesting that verbal memory improvement through time should be mostly attributed to PSAT. On the other hand, the cognitive speed improvement during follow-up should be attributed to both interventions.Conclusions: These findings provide important insight into the role of stress management. Future studies should focus on randomized, controlled trials with larger samples and longer follow-up times.","PeriodicalId":73836,"journal":{"name":"Journal of molecular biochemistry","volume":" ","pages":"22-31"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7842355/pdf/nihms-1661774.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25315989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential interference of aluminum chlorohydrate with estrogen receptor signaling in breast cancer cells. 氯化铝对乳腺癌细胞雌激素受体信号的潜在干扰。

Journal of molecular biochemistry Pub Date : 2018-01-01

Vyron A Gorgogietas, Ioannis Tsialtas, Natalie Sotiriou, Vasiliki C Laschou, Aikaterini G Karra, Demetres D Leonidas, George P Chrousos, Evagelia Protopapa, Anna-Maria G Psarra

{"title":"Potential interference of aluminum chlorohydrate with estrogen receptor signaling in breast cancer cells.","authors":"Vyron A Gorgogietas, Ioannis Tsialtas, Natalie Sotiriou, Vasiliki C Laschou, Aikaterini G Karra, Demetres D Leonidas, George P Chrousos, Evagelia Protopapa, Anna-Maria G Psarra","doi":"","DOIUrl":"","url":null,"abstract":"Aluminum salts are widely used as the active antiperspirant in underarm cosmetic. Experimental observations indicate that its long term application may correlate with breast cancer development and progression. This action is proposed to be attributed, among others, to aluminum possible estrogen-like activities. In this study we showed that aluminum, in the form of aluminum chlorohydrate (ACH), caused increase in estrogen receptor alpha (ERα) protein levels, in ERα-positive MCF-7 cells. This effect was accompanied by moderate activation of Estrogen Response Elements (ERE)-driven reporter gene expression and 20%-50% increase in certain estrogen responsive, ERE-independent genes expression. Genes affected were ERα, p53, cyclin D1, and c-fos, crucial regulators of breast cancer development and progression. ACH-induced genes expression was eliminated in the presence of the estrogen antagonist: ICI 182780, in MCF-7 cells, whereas it was not observed in ERα-negative MDA-MB-231 breast cancer cells, indicating aluminum interference with estrogen signaling. Moreover, ACH caused increase in the perinuclear localization of estrogen receptor alpha in MCF-7 breast cancer cells and increase in the mitochondrial Bcl-2 protein, possibly affecting receptors-mediated mitochondrial actions and mitochondrial-dependent apoptosis. ACH-induced perinuclear localization of estrogen receptor beta was also observed in MDA-MB-231. Our findings indicate that aluminum actions on estrogen receptors protein level and subcellular localization possibly affect receptors-mediated actions and thus, aluminum interference with estrogen signaling.","PeriodicalId":73836,"journal":{"name":"Journal of molecular biochemistry","volume":"7 1","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6108589/pdf/nihms-985242.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36430362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Introducing Thetis: a comprehensive suite for event detection in molecular dynamics. Thetis简介：一套全面的分子动力学事件检测套件。

Journal of molecular biochemistry Pub Date : 2018-01-01 Epub Date: 2018-10-03

Eleni Picasi, Athanasios Tartas, Vasileios Megalooikonomou, Dimitrios Vlachakis

引用次数: 0

Selective lactase deficiency is common in pediatric patients undergoing upper endoscopy. 选择性乳糖酶缺乏症在接受上腔镜检查的儿科患者中很常见。

Journal of molecular biochemistry Pub Date : 2018-01-01 Epub Date: 2018-05-31

Annie Goodwin, Lina Karam, G S Gopalakrishna, Richard Kellermayer

{"title":"Selective lactase deficiency is common in pediatric patients undergoing upper endoscopy.","authors":"Annie Goodwin, Lina Karam, G S Gopalakrishna, Richard Kellermayer","doi":"","DOIUrl":"","url":null,"abstract":"Lactase deficiency can lead to significant symptoms in the pediatric population. To date, few studies have examined the prevalence of enzyme testing-based lactase and other disaccharidase deficiencies (DDs) in pediatric patients undergoing upper endoscopic evaluation. The primary objective of this study was to determine the prevalence of selective lactase and other DDs amongst a large cohort of pediatric patients with and without inflammatory bowel disease (IBD: Crohn's disease and ulcerative colitis) via a chart review of 739 patients who underwent esophago-gastro-dudenoscopy EGD between April 2010 and August 2016. We identified 560 pediatric patients (ages 1-18 years) who underwent mucosal enzyme testing at the time of their EGD. The overall rate of lactase deficiency (LD) was 39%. LD positively correlated with age (p=0.00017), but there was no significant difference between age matched IBD and non-IBD patients (45% vs. 42% p=0.68). Four patients (0.17%) were found to have selective maltase deficiency. No selective sucrase or palatinase deficiency was identified. Statistically significant differences occurred in lactase deficiency amongst patients of different races. In conclusion, lactase deficiency is a relatively common finding in children undergoing EGD though at no increased rate amongst the IBD patient population. Disaccharidase testing should be considered in pediatric patients undergoing EGD.","PeriodicalId":73836,"journal":{"name":"Journal of molecular biochemistry","volume":"7 1","pages":"41-47"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6481960/pdf/nihms-1022934.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37192039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adenylyl cyclases and TRPV4 mediate Ca²⁺/cAMP dynamics to enhance fluid flow-induced osteogenesis in osteocytes. 腺苷酸环化酶和TRPV4介导Ca2+/cAMP动力学，以增强骨细胞中流体诱导的成骨。

Journal of molecular biochemistry Pub Date : 2018-01-01

Emily R Moore, Han Seul Ryu, Ya Xing Zhu, Christopher R Jacobs

{"title":"Adenylyl cyclases and TRPV4 mediate Ca2+/cAMP dynamics to enhance fluid flow-induced osteogenesis in osteocytes.","authors":"Emily R Moore, Han Seul Ryu, Ya Xing Zhu, Christopher R Jacobs","doi":"","DOIUrl":"","url":null,"abstract":"Bone adapts to physical forces and this process is dependent on osteocyte mechanotransduction. One way osteocytes sense mechanical stimulation is through the primary cilium, a sensory organelle that triggers intracellular signaling cascades in response to fluid shear. Our lab previously determined that flow-induced ciliary Ca2+ influx and changes in cytosolic cAMP levels are critical for osteogenesis. We also identified two proteins important for osteocyte mechanotransduction: transient receptor potential vanilloid 4 (TRPV4) and adenylyl cyclase 6 (AC6). Interestingly, disrupting the Ca2+-binding ability of these proteins results in loss of function. Although knockdowns of TRPV4 and AC6 disrupt osteogenesis, there is no definitive evidence linking them to Ca2+/cAMP dynamics that facilitate osteocyte mechanotransduction. We therefore transfected MLO-Y4 osteocytes with AC3/6 and TRPV4 overexpression plasmids that fail to interact with Ca2+ and observed the response to fluid shear. Indeed, mutant groups exhibited adverse changes in cAMP and lower mRNA expression of an osteogenic marker, COX-2, at the onset of flow. This pattern persisted for AC3 and TRPV4 but we detected no difference in AC6 at longer exposure to flow. These results suggest TRPV4 and ACs mediate Ca2+/cAMP dynamics that are important for osteocyte mechanotransduction. These mechanisms are potential targets for therapeutics to combat bone loss and should be investigated further.","PeriodicalId":73836,"journal":{"name":"Journal of molecular biochemistry","volume":"7 ","pages":"48-59"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6529196/pdf/nihms-990134.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37270840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0