Advances in Human Biology最新文献

Rethinking the Efficacy of Natural and Synthetic Folic Acid on Human Health and Looking into a Better Alternative 反思天然和合成叶酸对人体健康的功效，寻找更好的替代品

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Advances in Human Biology Pub Date : 2024-01-02 DOI: 10.4103/aihb.aihb_144_23

Rahnuma Ahmad, Mainul Haque

引用次数: 0

Dengue Dynamics: A Global Update 登革热动态：全球最新情况

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Advances in Human Biology Pub Date : 2023-12-26 DOI: 10.4103/aihb.aihb_135_23

R. Yousuf, Md Wasek Salam, Shaima Akter, Susmita Sinha, Mainul Haque

引用次数: 0

Effective Programmes to Reduce Inappropriate Dispensing of Antibiotics in Community Pharmacies Especially in Developing Countries 减少社区药房（尤其是发展中国家的社区药房）抗生素不当配药的有效方案

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Advances in Human Biology Pub Date : 2023-12-26 DOI: 10.4103/aihb.aihb_128_23

Tiyani Milta Sono, Vanda Marković-Peković, Brian Godman

引用次数: 0

Study of the cellular senescence process in human umbilical cord Wharton's jelly-derived mesenchymal stem cells 人脐带沃顿氏胶状间充质干细胞细胞衰老过程的研究

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Advances in Human Biology Pub Date : 2023-10-01 DOI: 10.4103/aihb.aihb_19_23

S. Hejazi, M. Maleki, Morteza Rasekh

{"title":"Study of the cellular senescence process in human umbilical cord Wharton's jelly-derived mesenchymal stem cells","authors":"S. Hejazi, M. Maleki, Morteza Rasekh","doi":"10.4103/aihb.aihb_19_23","DOIUrl":"https://doi.org/10.4103/aihb.aihb_19_23","url":null,"abstract":"Introduction: Embryonic stem cells are candidates for the treatment of regenerative medicine, but their use is faced with limitations due to ethical issues. The human umbilical cord-derived mesenchymal stem cells (MSCs) are appropriate options because the cells have no ethical difficulties and have self-renewal. Senescence is known as a gradual functional loss that heterogeneously occurs in multiple organ systems. Objective: The objective of this study was to investigate the cellular senescence process in human umbilical cord Wharton's jelly-derived mesenchymal cells. Materials and Methods: Umbilical cord was obtained from healthy newborns at the General Hospital of Tabriz. Under sterile conditions, Wharton's jelly was removed from the blood vessels and minced into small pieces of about 0.5 mm. These were cultured in Dulbecco's Modified Eagle's Medium (MSC medium). Real-time polymerase chain reaction for p16INK4a and senescence-associated β-galactosidase (SA-β-gal) staining was performed to investigate the cellular senescence process. Results: The results showed the different expressions in the different passages, but it was significantly increased from the fifth passage compared to the first passage. SA-β-gal staining also showed increased colour intensity in the fifth passage compared to the first passage. Conclusion: SA-β-gal is not a specific marker for senescence, while p16INK4a is a specific marker. Further studies are required for the investigation of the senescence mechanism, such as the evaluation of genes involved in the senescence.","PeriodicalId":7341,"journal":{"name":"Advances in Human Biology","volume":"11 1","pages":"361 - 366"},"PeriodicalIF":0.4,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70643802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Expression of BAX and Bcl-2 gene in prostate carcinoma and its correlation with Gleason score 前列腺癌中BAX、Bcl-2基因的表达及其与Gleason评分的关系

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Advances in Human Biology Pub Date : 2023-10-01 DOI: 10.4103/aihb.aihb_46_23

Subhashis Das, Aiswarya Unnithan, S. Nadipanna

{"title":"Expression of BAX and Bcl-2 gene in prostate carcinoma and its correlation with Gleason score","authors":"Subhashis Das, Aiswarya Unnithan, S. Nadipanna","doi":"10.4103/aihb.aihb_46_23","DOIUrl":"https://doi.org/10.4103/aihb.aihb_46_23","url":null,"abstract":"Introduction: Prostate cancer is primarily a disease that occurs in the elderly age group amongst men above 65 years of age. The relationship between the Bcl-2 and Bax genes and the Gleason staging of prostate cancer has not been studied in depth, and there is a dearth of published Indian research on the subject. Hence, this study aims to comprehend how the apoptotic mechanism relates to prostate cancer. The aim of the study was to determine the proportion (expression) of Bcl-2 and BAX genes in prostate carcinoma (PCa) and to observe the correlation of Bcl-2 and BAX gene association with Gleason scoring in PCa. Materials and Methods: Transurethral resected prostatic Chips positive for PCa and paraffin blocks were evaluated for the study. H-score system was used based on the cytoplasmic staining into negative mild, moderate and strong cytoplasmic staining. Results: Fifty cases were analysed. There was a notable relationship between Gleason grade and tumour size, and levels of prostate-specific antigen (PSA) were in an increasing manner with Gleason's grade except at Grade 3, with the majority of cases falling into Gleason grade 5 (42%) and PSA levels falling into the range of 90–99 (28%), tumour size falling into the range of 3.1–6 cm (44%), T4 stage (34%) and metastasis (80%). Conclusion: This study concluded that PSA levels in cases of prostate cancer patients were showing an increasing trend with Gleason grade. This demonstrated a striking correlation between Gleason grade and H score BCL2, but not one that was particularly significant for the BAX gene.","PeriodicalId":7341,"journal":{"name":"Advances in Human Biology","volume":"1 1","pages":"344 - 349"},"PeriodicalIF":0.4,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70645247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Evaluation of invasive tumour front in primary and secondary oral squamous cell carcinoma – A reliable prognostic parameter 评估原发性和继发性口腔鳞状细胞癌侵袭性肿瘤前缘-一个可靠的预后参数

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Advances in Human Biology Pub Date : 2023-10-01 DOI: 10.4103/aihb.aihb_158_22

Hemal Joshi, Neelampari Parikh, C. Nandini, G. Bhatt, Sandeep Jain, Ashwini Parmar

引用次数: 0

SARS-CoV-2 encodes ORF8a as viroporin and ORF8b as NLRP3 activator? SARS-CoV-2将ORF8a编码为病毒孔蛋白，ORF8b编码为NLRP3激活剂?

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Advances in Human Biology Pub Date : 2023-07-01 DOI: 10.4103/aihb.aihb_206_22

Parisa Zeynali, Vahid Tambrchi, Emad Behboudi

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Repurposing the anti-depression drug sertraline against catheter-associated urinary tract infections 重新利用抗抑郁药物舍曲林治疗导尿管相关性尿路感染

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Advances in Human Biology Pub Date : 2023-07-01 DOI: 10.4103/aihb.aihb_9_23

M. Poyil, Mohammed Khan, Meiyazhagan Gowri

{"title":"Repurposing the anti-depression drug sertraline against catheter-associated urinary tract infections","authors":"M. Poyil, Mohammed Khan, Meiyazhagan Gowri","doi":"10.4103/aihb.aihb_9_23","DOIUrl":"https://doi.org/10.4103/aihb.aihb_9_23","url":null,"abstract":"Introduction: Catheter-associated urinary tract infections (CAUTIs) account for one-third of the nosocomial infections. As drug repurposing is an excellent method to develop new antimicrobials, the present study is a trial to repurpose the anti-depression drug sertraline against two of the major CAUTI causing, biofilm forming bacteria viz., Enterococcus faecalis and Escherichia coli. Materials and Methods: Agar diffusion and microdilution methods were used in repurposing sertraline against E. faecalis and E. coli, to find out the antibacterial activity and the minimal inhibitory concentration of the drug. The anti-biofilm activity of the drug was also studied. Results: Sertraline (concentrations given within brackets) produced growth inhibition zones against E. faecalis as: 23 mm (125 μg), 24 mm (150 μg) and 26 mm (200 μg) and against E. coli as: 14 mm (125 μg), 15 mm (150 μg) and 17 mm (200 μg), respectively. Against both of the pathogens, the minimal inhibitory concentration of the drug was found to be 75 μg/ml, and at this concentration, the drug prevented the colony formation by these bacteria on polystyrene surfaces. The drug reduced the biofilms of E. faecalis and E. coli by 62% and 69%, respectively. Conclusion: When repurposed against E. faecalis and E. coli sertraline showed promising antibacterial, anti-biofilm and anti-adhesive activities, repurposing being one of the easiest ways to develop new drugs against bacterial pathogens, the authors suggest for further studies on repurposing sertraline for its clinical applications.","PeriodicalId":7341,"journal":{"name":"Advances in Human Biology","volume":"13 1","pages":"271 - 276"},"PeriodicalIF":0.4,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42669758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The vital role of forensic medicine as a 'hidden curriculum' in medical education: Current perspectives 法医学作为一门“隐性课程”在医学教育中的重要作用：当前视角

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Advances in Human Biology Pub Date : 2023-07-01 DOI: 10.4103/aihb.aihb_61_23

Mainul Haque, N. Razak

引用次数: 0

Pros and cons of air-dried and conventional wet-fixed Pap smears: An institutional study 风干和常规湿法子宫颈抹片检查的利弊:一项制度研究

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Advances in Human Biology Pub Date : 2023-07-01 DOI: 10.4103/aihb.aihb_15_23

A. Mary, Subhashish Das, S. Sheela

{"title":"Pros and cons of air-dried and conventional wet-fixed Pap smears: An institutional study","authors":"A. Mary, Subhashish Das, S. Sheela","doi":"10.4103/aihb.aihb_15_23","DOIUrl":"https://doi.org/10.4103/aihb.aihb_15_23","url":null,"abstract":"Introduction: Wet-fixed smear study routinely used to identify several pre-cancerous and cancerous tumours. By contrasting cellular preservation and staining quality in rehydration smears with wet-fixed smears, the current study aims to establish the effects of rehydration on air-dried smears. Materials and Methods: One hundred paired samples are compared. Wet fixing was done in the first place. Air-dried samples are rehydrated for 30 s with regular saline and then placed in 95% ethanol for 30 min. Both smears will undergo screening, evaluation and grading for numerous cytomorphological factors after Pap staining. The 2014 Bethesda system was used to report the cervical smear results. Results: When evaluated for numerous cellular properties, statistically significant similarities can be seen between two groups, one with wet-fixed smears and the other with rehydrated air-dried smears. The results of the cytological diagnosis comparison between the two groups can be shown in the agreement. Rehydrated air-dried smear interpretation and staining quality are significantly superior to traditional wet smears. Conclusions: Rehydration air-dried techniques can be used instead of traditional wet fixation methods and are as effective when used alone or with traditional wet-fixed smears.","PeriodicalId":7341,"journal":{"name":"Advances in Human Biology","volume":"13 1","pages":"277 - 280"},"PeriodicalIF":0.4,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42067240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0