In vitro models最新文献_第3页

Fracture mechanism and mechanical properties of porous HA/PLCL and HA/PLLA sandwich composite beams 多孔HA/PLCL和HA/PLLA夹层复合梁断裂机理及力学性能

In vitro models Pub Date : 2023-07-13 DOI: 10.1007/s44164-023-00053-0

Fatin Hazwani, Aiman Izmin, M. Todo

引用次数: 0

Alveolar epithelial-like cell differentiation in a dynamic bioreactor: a promising 3D-approach for the high-throughput generation of lung cell types from human induced pluripotent stem cells 动态生物反应器中的肺泡上皮样细胞分化:从人诱导多能干细胞中高通量生成肺细胞类型的有前途的3d方法

In vitro models Pub Date : 2023-06-29 DOI: 10.1007/s44164-023-00052-1

M. Müller, Y. Kohl, A. Germann, Sylvia Wagner, H. Zimmermann, H. von Briesen

引用次数: 0

Stainless steel and titanium alloys coated with sulfated chitosan to improve hemocompatibility properties 不锈钢和钛合金包覆硫酸壳聚糖，改善血液相容性

In vitro models Pub Date : 2023-06-14 DOI: 10.1007/s44164-023-00044-1

S. Gomes, M. Arcanjo, Francisca Raysse Mesquita Silva, Luzia Kalyne Almeida Santos Moreira Leal, A. Claro, K. Popat, R. Vieira

引用次数: 0

Longitudinally aligned inner-patterned silk fibroin conduits for peripheral nerve regeneration 纵向排列内图案丝素蛋白导管用于周围神经再生

In vitro models Pub Date : 2023-04-18 DOI: 10.1007/s44164-023-00050-3

Ane Escobar, M. Carvalho, T. H. Silva, R. Reis, J. Oliveira

引用次数: 0

Nano-hydroxyapatite/natural polymer composite scaffolds for bone tissue engineering: a brief review of recent trend 纳米羟基磷灰石/天然高分子复合材料骨组织工程支架研究进展

In vitro models Pub Date : 2023-04-13 DOI: 10.1007/s44164-023-00049-w

G. Radha, N. Manjubaashini, S. Balakumar

引用次数: 1

A human Caco-2-based co-culture model of the inflamed intestinal mucosa for particle toxicity studies 用于颗粒毒性研究的基于caco -2的人炎症肠黏膜共培养模型

In vitro models Pub Date : 2023-03-24 DOI: 10.1007/s44164-023-00047-y

M. Paul, Marén Schlief, Hannes Daher, A. Braeuning, H. Sieg, L. Böhmert

引用次数: 2

Correction: In situ 3D spatiotemporal measurement of soluble biomarkers in spheroid culture 校正:球体培养中可溶性生物标志物的原位三维时空测量

In vitro models Pub Date : 2023-02-09 DOI: 10.1007/s44164-023-00042-3

A. McGhee, E. McGhee, Jack E Famiglietti, W. Sawyer

引用次数: 0

Functional biomaterials for biomimetic 3D in vitro tumor microenvironment modeling 用于体外肿瘤微环境仿生三维建模的功能生物材料

In vitro models Pub Date : 2023-01-27 DOI: 10.1007/s44164-023-00043-2

T. Ahmed

引用次数: 1

In vitro cancer models as an approach to identify targetable developmental phenotypes in cancer stem cells. 体外癌症模型作为鉴定癌症干细胞靶向发育表型的方法。

In vitro models Pub Date : 2023-01-01 Epub Date: 2023-05-12 DOI: 10.1007/s44164-023-00051-2

Adrian Biddle

{"title":"In vitro cancer models as an approach to identify targetable developmental phenotypes in cancer stem cells.","authors":"Adrian Biddle","doi":"10.1007/s44164-023-00051-2","DOIUrl":"10.1007/s44164-023-00051-2","url":null,"abstract":"Cancer therapeutics are often highly toxic to the patient, and they often elicit rapid resistance in the tumour. Recent advances have suggested a potential new way in which we may improve on this, through two important concepts: (1) that multitudinous pathway alterations converge on a limited number of cancer cellular phenotypes, and (2) that these cancer cellular phenotypes depend on reactivation of developmental processes that are only minimally active in adult tissues. This provides a rationale for pursuing an approach of 'drugging the phenotype' focussed on targeting reactivated cellular processes from embryonic development. In this concepts paper, we cover these recent developments and their implications for the development of new cancer therapeutics that can avoid patient toxicity and acquired resistance. We then propose that in vitro tumour and developmental models can provide an experimental approach to identify and target the specific developmental processes at play, with a focus on the reactivation of developmental processes in the cancer stem cells that drive tumour progression and spread. Ultimately, the aim is to identify cellular processes that are specific to developmental phenotypes, are reactivated in cancer stem cells, and are essential to tumour progression. Therapeutically targeting these cellular processes could represent a new approach of 'drugging the phenotype' that treats the tumour whilst avoiding patient toxicity or the acquisition of therapeutic resistance.","PeriodicalId":73357,"journal":{"name":"In vitro models","volume":"2 3-4","pages":"83-88"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10550853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41123672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of a 3D hydrogel-based model to replace use of animals for passaging patient-derived xenografts. 基于3D水凝胶的模型的应用，以取代使用动物通过患者来源的异种移植物。

In vitro models Pub Date : 2023-01-01 Epub Date: 2023-05-09 DOI: 10.1007/s44164-023-00048-x

Sal Jones, Jennifer C Ashworth, Marian Meakin, Pamela Collier, Catherine Probert, Alison A Ritchie, Catherine L R Merry, Anna M Grabowska

{"title":"Application of a 3D hydrogel-based model to replace use of animals for passaging patient-derived xenografts.","authors":"Sal Jones, Jennifer C Ashworth, Marian Meakin, Pamela Collier, Catherine Probert, Alison A Ritchie, Catherine L R Merry, Anna M Grabowska","doi":"10.1007/s44164-023-00048-x","DOIUrl":"10.1007/s44164-023-00048-x","url":null,"abstract":"Purpose: This 3D in vitro cancer model for propagation of patient-derived cells, using a synthetic self-assembling peptide gel, allows the formation of a fully characterised, tailorable tumour microenvironment. Unlike many existing 3D cancer models, the peptide gel is inert, apart from molecules and motifs deliberately added or produced by cells within the model.Methods: Breast cancer patient-derived xenografts (PDXs) were disaggregated and embedded in a peptide hydrogel. Growth was monitored by microscopic examination and at intervals, cells were extracted from the gels and passaged on into fresh gels. Passaged cells were assessed by qPCR and immunostaining techniques for the retention of characteristic markers.Results: Breast cancer PDXs were shown to be capable of expansion over four or more passages in the peptide gel. Contaminating mouse cells were found to be rapidly removed by successive passages. The resulting human cells were shown to be compatible with a range of common assays useful for assessing survival, growth and maintenance of heterogeneity.Conclusions: Based on these findings, the hydrogel has the potential to provide an effective and practical breast cancer model for the passage of PDXs which will have the added benefits of being relatively cheap, fully-defined and free from the use of animals or animal products. Encapsulated cells will require further validation to confirm the maintenance of cell heterogeneity, genotypes and phenotypes across passage, but with further development, including the addition of bespoke cell and matrix components of the tumour microenvironment, there is clear potential to model other cancer types.Supplementary information: The online version contains supplementary material available at 10.1007/s44164-023-00048-x.","PeriodicalId":73357,"journal":{"name":"In vitro models","volume":"2 3-4","pages":"99-111"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10550889/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41171026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0