In vitro models最新文献_第2页

Identification and prediction of molecular factors associated with ischemic stroke: an integrative analysis of DEGs, TFs, and PPI networks 缺血性中风相关分子因素的识别与预测：DEGs、TFs 和 PPI 网络的综合分析

In vitro models Pub Date : 2023-12-01 DOI: 10.1007/s44164-023-00063-y

Mehran Radak, Hossein Fallahi

引用次数: 0

Leveraging mesoporous silica nanomaterial for optimal immunotherapeutics against cancer 利用介孔二氧化硅纳米材料的最佳免疫治疗癌症

In vitro models Pub Date : 2023-11-13 DOI: 10.1007/s44164-023-00061-0

K. C. Ashitha, Gopinath M, Sasirekha N.R, Balakumar S, Rajashree P

{"title":"Leveraging mesoporous silica nanomaterial for optimal immunotherapeutics against cancer","authors":"K. C. Ashitha, Gopinath M, Sasirekha N.R, Balakumar S, Rajashree P","doi":"10.1007/s44164-023-00061-0","DOIUrl":"https://doi.org/10.1007/s44164-023-00061-0","url":null,"abstract":"Cancer represents a significant cause of morbidity and mortality. Definitive chemotherapy, surgery and radiotherapy treatment have not improved the “5-year survival period” and have shown recurrence. Currently, cancer immunotherapy is reported to be a promising therapeutic modality that aims to potentiate immune response against cancer by employing immune checkpoint inhibitors, cancer vaccines and immunomodulators. Inhibition of immune checkpoints such as PD-1/PDL1, CTLA and TIM molecules using monoclonal antibodies, ligands or both are proven to be the most successful anticancer immunotherapy. But the application of immunotherapy involves critical challenges such as non-responsiveness and systemic toxicity due to the administration of high dose. To mitigate the above challenges, nanomaterial-based delivery and therapy have been adopted to inhibit the immune checkpoints and induce an anticancer immune response. Specifically, mesoporous silica-based materials for cancer therapy are shown to be versatile materials for the above purpose. Mesoporous silica nanoparticle (MSN) based cancer immunotherapy overcomes numerous challenges and offers novel strategies for improving conventional immunotherapies. MSN has a high surface area, porosity and biocompatibility; it also has natural immune-adjuvant properties, which have been reported to be the best candidate material for immunotherapeutic delivery. This review will focus on the use of MSN as carriers for delivering immune checkpoint inhibitors and their efficacy in cancer combination therapy.","PeriodicalId":73357,"journal":{"name":"In vitro models","volume":"61 43","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136283763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Special issue: recent trends in bionanomaterials for health care applications 特刊:医疗保健应用生物纳米材料的最新趋势

In vitro models Pub Date : 2023-11-07 DOI: 10.1007/s44164-023-00062-z

Subramaina Balakumar, Vijayakumari Sugumaran

引用次数: 0

Complex in vitro models: do not complicate it 复杂的体外模型:不要使其复杂化

In vitro models Pub Date : 2023-09-27 DOI: 10.1007/s44164-023-00060-1

J. Miguel Oliveira

引用次数: 0

Development of a tissue-engineered skin model with epidermal, dermal and hypodermal components 组织工程皮肤模型的发展与表皮，真皮和皮下成分

In vitro models Pub Date : 2023-09-21 DOI: 10.1007/s44164-023-00058-9

V. L. Workman, A-V. Giblin, N. H. Green, S. MacNeil, V. Hearnden

{"title":"Development of a tissue-engineered skin model with epidermal, dermal and hypodermal components","authors":"V. L. Workman, A-V. Giblin, N. H. Green, S. MacNeil, V. Hearnden","doi":"10.1007/s44164-023-00058-9","DOIUrl":"https://doi.org/10.1007/s44164-023-00058-9","url":null,"abstract":"Abstract Tissue-engineered models of skin have evolved over the past 50 years, have successfully been translated to clinical use and continue to be improved using new technologies. However, very few of these constructs incorporate a hypodermal component. The hypodermis is critical to skin homeostasis, skin function and many skin diseases, but our understanding of the hypodermis is limited in comparison to our knowledge of the epidermis and dermis, in part due to a lack of suitable in vitro models. The purpose of this study was to develop and characterise a tissue-engineered model of skin consisting of epidermal, dermal and hypodermal layers, namely a trilayer skin model. Models were produced by culturing human keratinocytes and fibroblasts on decellularised human dermis in combination with explanted human adipose tissue. Bilayer models of skin, comprising of an epidermis and dermis, had a thicker epidermal component compared to trilayer models but exhibited similar cytokeratin expression patterns (AE1/AE3 and cytokeratin 14). Addition of adipose tissue improved the appearance of the dermal-epidermal junction, increased the number of rete ridge-like features and cells maintained similar levels of proliferation (Ki-67) compared to native tissues over 28 days in culture. This technique enabled us to create a physiologically relevant model of human skin with representative morphology across the hypodermis, dermis and epidermis. This model maintained native extracellular matrix architecture, contained a heterogeneous population of cells and has the potential to be applied to a range of different applications where research questions require the inclusion of a hypodermis.","PeriodicalId":73357,"journal":{"name":"In vitro models","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136154104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D tumor spheroids: morphological alterations a yardstick to anti-cancer drug response 三维肿瘤球体:形态改变是抗癌药物反应的标尺

In vitro models Pub Date : 2023-09-20 DOI: 10.1007/s44164-023-00059-8

Anna Senrung, Sakshi Lalwani, Divya Janjua, Tanya Tripathi, Jasleen Kaur, Netra Ghuratia, Nikita Aggarwal, Arun Chhokar, Joni Yadav, Apoorva Chaudhary, Udit Joshi, Alok Chandra Bharti

{"title":"3D tumor spheroids: morphological alterations a yardstick to anti-cancer drug response","authors":"Anna Senrung, Sakshi Lalwani, Divya Janjua, Tanya Tripathi, Jasleen Kaur, Netra Ghuratia, Nikita Aggarwal, Arun Chhokar, Joni Yadav, Apoorva Chaudhary, Udit Joshi, Alok Chandra Bharti","doi":"10.1007/s44164-023-00059-8","DOIUrl":"https://doi.org/10.1007/s44164-023-00059-8","url":null,"abstract":"Tumor spheroids are one of the well-characterized 3D culture systems bearing close resemblance to the physiological tissue organization and complexity of avascular solid tumor stage with hypoxic core. They hold a wide-spread application in the field of pharmaceutical science and anti-cancer drug research. However, the difficulty in determining optimal technique for the generation of spheroids with uniform size and shape, evaluation of experimental outputs, or mass production often limits their usage in anti-cancer research and in high-throughput drug screening. In recent times, several studies have demonstrated various simple techniques for generating uniform-size 3D spheroids, including the hanging drop (HD), liquid overlay technique (LOT), and microfluidic approaches. Morphological alterations apart from biochemical assays, and staining techniques are suitably employed for the evaluation of experimental outcomes within 3D spheroid models. Morphological alterations in response to effective anti-cancer drug treatment in 3D tumor spheroids such as reduced spheroid size, loss of spheroid compactness and integrity or smooth surface, are highly reliable. These alterations can significantly reduce the need for biochemical assays and staining techniques, resulting in both time and cost savings. The present article specifically covers a variety of available procedures in spheroid generation. For practical applicability, we have supplemented our review study with the generation of glioblastoma U87 spheroids using HD and LOT methods. Additionally, we have also incorporated the outcome of U87 spheroid treatment with doxorubicin on spheroid morphology.","PeriodicalId":73357,"journal":{"name":"In vitro models","volume":"89 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136375228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ex vivo models for intestinal translocation studies of cellulose nanocrystals 纤维素纳米晶体肠道易位研究的离体模型

In vitro models Pub Date : 2023-08-21 DOI: 10.1007/s44164-023-00056-x

M. Müller, R. Drexel, Marie Burkhart, S. Dähnhardt-Pfeiffer, Lena Wien, C. Herrmann, Thorsten Knoll, C. Metzger, H. Briesen, Sylvia Wagner, F. Meier, Y. Kohl

引用次数: 0

An original donor-dependent spheroid system for the prediction of idiosyncratic drug-induced liver injury risk 用于预测特异性药物性肝损伤风险的原始供体依赖球体系统

In vitro models Pub Date : 2023-08-15 DOI: 10.1007/s44164-023-00057-w

S. Cherradi, Nicolas Taulet, Hong T Duong

引用次数: 0

The protective effect of endurance running against the pro-invasive effects of ageing in breast cancer cells and mesenchymal stem cells in vitro 在体外实验中，耐力跑对乳腺癌细胞和间充质干细胞抗衰老的保护作用

In vitro models Pub Date : 2023-08-02 DOI: 10.1007/s44164-023-00055-y

Marie-Juliet Brown, Matt Nickels, E. Akam, Mhairi A. Morris

引用次数: 0

Microfluidics engineering towards personalized oncology—a review 面向个性化肿瘤学的微流体工程综述

In vitro models Pub Date : 2023-07-13 DOI: 10.1007/s44164-023-00054-z

Sushmita Mishra, M. Kumarasamy

引用次数: 0