Immunology and immunogenetics insights最新文献

Association Between IL16 Gene Polymorphism and Allergic Contact Dermatitis Among Construction Workers IL16基因多态性与建筑工人过敏性接触性皮炎的相关性研究

Immunology and immunogenetics insights Pub Date : 2019-01-01 DOI: 10.1177/1178634519880556

E. Khashaba, M. Gaballah, A. State, Mona Elwassefy

{"title":"Association Between IL16 Gene Polymorphism and Allergic Contact Dermatitis Among Construction Workers","authors":"E. Khashaba, M. Gaballah, A. State, Mona Elwassefy","doi":"10.1177/1178634519880556","DOIUrl":"https://doi.org/10.1177/1178634519880556","url":null,"abstract":"Objectives: First, this research was instituted to identify common allergens, and second, to test the association between IL16 gene promoter polymorphism rs4778889 T/C and allergic contact dermatitis (ACD). Methods: A case control study was conducted in dermatology outpatients’ clinic. Study subjects received interview-based semi-structured questionnaire, complete skin examination, IL16 gene promoter was investigated by PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) analysis, and IQ Ultra™ patch test units (Chemotechnique Diagnostics AB, Sweden) with 10 substances were used. Results: Most of the prevalent cases had positive patch test (93.3%). The most common clinical presentation of ACD in our patients was itching (96.7%), followed by dryness (86.75%), erythema (76.7%), and fissuring (76.7%). There was higher CC gene distribution among cases, but there was no statistically significant difference. IL16 gene distribution was nearly similar among different clinical presentations. Formaldehyde showed statistically significant higher frequency for CC. The most common allergen found was mercury chloride (76.6%), followed by potassium dichromate (26.6%) and cobalt chloride (20%). Conclusions: The current study found prominent metal sensitization (mercury chloride) over the previously known potassium dichromate. There was no statistically significant IL16 gene distribution among cases compared with control. However, C allele was more frequently encountered in cases. Further studies are required to test the association with IL16 genotype and ACD and highlight the new trends in metal sensitization among cement-exposed workers.","PeriodicalId":73345,"journal":{"name":"Immunology and immunogenetics insights","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1178634519880556","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47772825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acute Hereditary Angioedema With Airway Compromise: Avoiding Airway Intervention With C1 Inhibitor Concentrate 急性遗传性血管性水肿伴气道损害：避免使用C1抑制剂浓缩物进行气道干预

Immunology and immunogenetics insights Pub Date : 2018-07-11 DOI: 10.1177/1178634518786254

Wei Fu, Sze-Chin Tan, E. Ho

引用次数: 0

Involvement of Immune Regulation in Multiple Sclerosis 免疫调节在多发性硬化症中的作用

Immunology and immunogenetics insights Pub Date : 2017-10-03 DOI: 10.1177/1178634517734175

Gabrielle Spagnuolo, Aaron Piavis, T. Williams

{"title":"Involvement of Immune Regulation in Multiple Sclerosis","authors":"Gabrielle Spagnuolo, Aaron Piavis, T. Williams","doi":"10.1177/1178634517734175","DOIUrl":"https://doi.org/10.1177/1178634517734175","url":null,"abstract":"Multiple sclerosis (MS) is a neurodegenerative disease characterized by neuroinflammation and demyelination that results in axon loss. Multiple sclerosis has been shown to be the result of an autoimmune response caused by a mixture of genetic and environmental factors. Dendritic cells are prominent antigen-presenting cells that interact with various molecules to regulate the immune system. The dysfunction of various features of immune regulation, including interleukins (ILs), CD4+ T cells, and suppressor of cytokine signaling (SOCS1), has been implicated in the pathogenesis of MS. T cells, particularly through the malfunction of B7-costimulatory pathways, have been shown to affect the progression of the disease. SOCS1 is important in regulating the function of T cells through its interactions with other nearby genes, especially CLEC16A, with abnormal decreases in SOCS1 expression leading to the exhibition of MS symptoms. The activation of IL-23 receptors on CD4+ T cells is pivotal to their differentiation into pathogenic TH17 cells. Several promising compounds that downregulate gene expression of IL-23 and IL-23R have been discovered but require further investigation for efficacy and safety. Given their role in the severity and progression of MS, therapies that decrease these dysregulations may ultimately decrease symptoms and in turn improve patients’ quality of life.","PeriodicalId":73345,"journal":{"name":"Immunology and immunogenetics insights","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1178634517734175","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47917582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Genotype Frequencies of Transient Receptor Potential Melastatin M3 Ion Channels and Acetylcholine Muscarinic M3 Receptor Gene Polymorphisms in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis Patients 慢性疲劳综合征/肌痛性脑脊髓炎患者瞬时受体电位美拉他汀M3离子通道基因型频率及乙酰胆碱毒蕈碱M3受体基因多态性

Immunology and immunogenetics insights Pub Date : 2016-01-01 DOI: 10.4137/III.S37042

S. Marshall-Gradisnik, A. Chacko, S. Johnston, P. Smith, B. Nilius, D. Staines

引用次数: 2

Immunity against Fungal Infections 抗真菌感染的免疫力

Immunology and immunogenetics insights Pub Date : 2016-01-01 DOI: 10.4137/III.S38707

Shuai Jiang

引用次数: 14

Examination of Single Nucleotide Polymorphisms in Acetylcholine Receptors in Chronic Fatigue Syndrome Patients 慢性疲劳综合征患者乙酰胆碱受体单核苷酸多态性的研究

Immunology and immunogenetics insights Pub Date : 2015-01-01 DOI: 10.4137/III.S25105

S. Marshall-Gradisnik, Peter Smith, B. Nilius, D. Staines

{"title":"Examination of Single Nucleotide Polymorphisms in Acetylcholine Receptors in Chronic Fatigue Syndrome Patients","authors":"S. Marshall-Gradisnik, Peter Smith, B. Nilius, D. Staines","doi":"10.4137/III.S25105","DOIUrl":"https://doi.org/10.4137/III.S25105","url":null,"abstract":"Objective Chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) is a disorder characterized by debilitating fatigue accompanied by pain and impairments in memory, cognition, and concentration. Acetylcholine (ACh) has a plethora of roles in neuronal and neuromuscular transmission. There are two types of ACh receptors, muscarinic and nicotinic, comprising 17 different subunits of the nicotinic ACh receptor (nAChR) and five different subtypes of the muscarinic receptor (mAChR) that have been identified in humans. The purpose of this study was to determine the role of ACh receptor (nAChRs and mAChRs) single nucleotide polymorphisms (SNPs) in CFS/ME patients. Methods One-hundred and fifteen CFS/ME patients (age = 48.68 ± 1.06 years) and 90 nonfatigued controls (age = 46.48 ± 1.22 years) participated in this study, where CFS/ME patients were defined according to the 1994 Center for Disease Prevention and Control (CDC) criteria. A total of 464 SNPs for nine mammalian ACh receptor genes (M1, M2, M3, M4, M5, alpha 2, 5, 7, and 10) were examined via the Agena Biosciences iPLEX Gold assay. Statistical analysis was performed using the PLINK analysis software. Results Seventeen SNPs were significantly associated with CFS/ME patients compared with the controls. Nine of these SNPs were associated with mAChRM3 (rs4463655; P = 0.00281, rs589962; P = 0.00348, rs1072320; P = 0.00371, rs7543259; P = 0.00513, rs6661621; P = 0.00536 rs7520974; P = 0.0167, rs726169; P = 0.02349, rsrs6669810; P = 0.02361, rsrs6429157; P = 0.0375), while the remainder were associated with nAChR alpha 10 (rs2672211; P = 0.0107, rs2672214; P = 0.0108, rs2741868; P = 0.01185, rs2741870; P = 0.01281, rs2741862; P = 0.03043), alpha 5 (rs951266; P = 0.01153; rs7180002, P = 0.03682), and alpha 2 (rs2565048; P = 0.01403). Conclusion The data from this pilot study suggest an association between ACh receptors, predominantly M3 and CFS. ACh receptor SNPs may contribute to the pathomechanism of CFS/ME.","PeriodicalId":73345,"journal":{"name":"Immunology and immunogenetics insights","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/III.S25105","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70703925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Characterization of Plasmodium falciparum Proteome at Asexual Blood Stages for Screening of Effective Vaccine Candidates: An Immunoinformatics Approach 恶性疟原虫蛋白质组在无性血阶段的特征，筛选有效的候选疫苗:免疫信息学方法

Immunology and immunogenetics insights Pub Date : 2015-01-01 DOI: 10.4137/III.S24755

S. P. Singh, V. Verma, B. Mishra

{"title":"Characterization of Plasmodium falciparum Proteome at Asexual Blood Stages for Screening of Effective Vaccine Candidates: An Immunoinformatics Approach","authors":"S. P. Singh, V. Verma, B. Mishra","doi":"10.4137/III.S24755","DOIUrl":"https://doi.org/10.4137/III.S24755","url":null,"abstract":"Malaria is a complex parasitic disease that is currently causing great concerns globally owing to the resistance to antimalarial drugs and lack of an effective vaccine. The present study involves the characterization of extracellular secretory proteins as vaccine candidates derived from proteome analysis of Plasmodium falciparum at asexual blood stages of malaria. Among the screened 32 proteins, 31 were predicted as antigens by the VaxiJen program, and 26 proteins had less than two transmembrane spanning regions predicted using the THMMM program. Moreover, 10 and 5 proteins were predicted to contain secretory signals by SignalP and TargetP, respectively. T-cell epitope prediction using MULTIPRED2 and NetCTL programs revealed that most of the predicted antigens are immunogenic and contain more than 10% supertype and 5% promiscuous epitopes of HLA-A, -B, or -DR. We anticipate that T-cell immune responses against asexual blood stages of Plasmodium are dispersed on a relatively large number of parasite antigens. This is the first report, to the best of our knowledge, offering new insights, at the proteome level, for the putative screening of effective vaccine candidates against the malaria pathogen. The findings also suggest new ways forward for the modern omics-guided vaccine target discovery using reverse vaccinology.","PeriodicalId":73345,"journal":{"name":"Immunology and immunogenetics insights","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/III.S24755","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70704036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Examination of Single Nucleotide Polymorphisms (SNPs) in Transient Receptor Potential (TRP) Ion Channels in Chronic Fatigue Syndrome Patients 慢性疲劳综合征患者瞬时受体电位(TRP)离子通道单核苷酸多态性(snp)的检测

Immunology and immunogenetics insights Pub Date : 2015-01-01 DOI: 10.4137/III.S25147

S. Marshall-Gradisnik, Peter Smith, E. Brenu, B. Nilius, S. Ramos, D. Staines

{"title":"Examination of Single Nucleotide Polymorphisms (SNPs) in Transient Receptor Potential (TRP) Ion Channels in Chronic Fatigue Syndrome Patients","authors":"S. Marshall-Gradisnik, Peter Smith, E. Brenu, B. Nilius, S. Ramos, D. Staines","doi":"10.4137/III.S25147","DOIUrl":"https://doi.org/10.4137/III.S25147","url":null,"abstract":"Background The transient receptor potential (TRP) superfamily in humans comprises 27 cation channels with permeability to monovalent and divalent cations. These channels are widely expressed within humans on cells and tissues and have significant sensory and regulatory roles on most physiological functions. Chronic fatigue syndrome (CFS) is an unexplained disorder with multiple physiological impairments. OBJECTIVES The purpose of this study was to determine the role of TRPs in CFS. Methods The study comprised 115 CFS patients (age = 48.68 ± 1.06 years) and 90 nonfatigued controls (age = 46.48 ± 1.22 years). CFS patients were defined according to the 1994 Center for Disease Prevention and Control criteria for CFS. A total of 240 single nucleotide polymorphisms (SNPs) for 21 mammalian TRP ion channel genes (TRPA1, TRPC1, TRPC2, TRPC3, TRPC4, TRPC6, TRPC7, TRPM1, TRPM2, TRPM3, TRPM4, TRPM5, TRPM6, TRPM7, TRPM8, TRPV1, TRPV2, TRPV3, TRPV4, TRPV5, and TRPV6) were examined via the Agena Biosciences iPLEX Gold assay. Statistical analysis was performed using the PLINK analysis software. Results Thirteen SNPs were significantly associated with CFS patients compared with the controls. Nine of these SNPs were associated with TRPM3 (rs12682832; P < 0.003, rs11142508; P < 0.004, rs1160742; P < 0.08, rs4454352; P < 0.013, rs1328153; P < 0.013, rs3763619; P < 0.014, rs7865858; P ≤ 0.021, rs1504401; P ≤ 0041, rs10115622; P ≤ 0.050), while the remainder were associated with TRPA1 (rs2383844; P ≤ 0.040, rs4738202; P ≤ 0.018) and TRPC4 (rs6650469; P ≤ 0.016, rs655207; P ≤ 0.018). Conclusion The data from this pilot study suggest an association between TRP ion channels, predominantly TRPM3 and CFS. This and other TRPs identified may contribute to the etiology and pathomechanism of CFS.","PeriodicalId":73345,"journal":{"name":"Immunology and immunogenetics insights","volume":"89 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/III.S25147","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70703624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

HLA-A, -B, -C, -DRB1, and -DQB1 Allele Lineages and Haplotype Frequencies among Saudis 沙特人HLA-A， -B， -C， -DRB1和-DQB1等位基因谱系和单倍型频率

Immunology and immunogenetics insights Pub Date : 2014-01-01 DOI: 10.4137/III.S16769

A. Osman, F. Gonzalez-Galarza, M. Mubasher, H. Alharthi, N. ElSheikh, N. Berka, D. Middleton, G. Elghazali

{"title":"HLA-A, -B, -C, -DRB1, and -DQB1 Allele Lineages and Haplotype Frequencies among Saudis","authors":"A. Osman, F. Gonzalez-Galarza, M. Mubasher, H. Alharthi, N. ElSheikh, N. Berka, D. Middleton, G. Elghazali","doi":"10.4137/III.S16769","DOIUrl":"https://doi.org/10.4137/III.S16769","url":null,"abstract":"There are few reported studies on Saudi population for human leukocyte antigens (HLA) genes. We investigated allele lineages (two-digit) and haplotype frequencies of HLA-A, -B, -C, -DRB1, and -DQB1 loci in 499 healthy unrelated individuals, selected from potential bone marrow transplant (BMT) families’ donors at King Fahad Medical City (KFMC), Saudi Arabia (SA). Genotyping was performed by Sequence Specific Oligonucleotide Probe (SSOP) utilizing a Luminex-based method. Allele lineages and haplotype frequencies were evaluated along with principal component analysis (PCA) to compare findings with previously reported data on Arab related populations. A total of 18 allele lineages for HLA-A, 28 for -B, 14 for -C, 13 for -DRB1, and 5 for -DQB1 were detected. High values for linkage disequilibrium indicators were found for B:C (D’ = 0.86599) and DRB1:DQB1 (D’ = 0.89468) loci. Additionally, PCA results confirmed previous findings on this population, but also indicated some genetic distances from other Arab related populations. The present study helps in further investigations of this population in anthropological analysis and HLA-associated disease studies.","PeriodicalId":73345,"journal":{"name":"Immunology and immunogenetics insights","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/III.S16769","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70703792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Cellular Responses to Cytosolic Double-stranded RNA–-The Role of the Inflammasome 细胞对胞质双链RNA的反应——炎症小体的作用

Immunology and immunogenetics insights Pub Date : 2014-01-01 DOI: 10.4137/III.S17839

A. Idris

引用次数: 5