GigaByte (Hong Kong, China)最新文献_第8页

The Crown Pearl V2: an improved genome assembly of the European freshwater pearl mussel Margaritifera margaritifera (Linnaeus, 1758). 皇冠珍珠 V2：欧洲淡水珍珠贻贝 Margaritifera margaritifera (Linnaeus, 1758) 的改进基因组组装。

GigaByte (Hong Kong, China) Pub Date : 2023-05-15 eCollection Date: 2023-01-01 DOI: 10.46471/gigabyte.81

André Gomes-Dos-Santos, Manuel Lopes-Lima, André M Machado, Thomas Forest, Guillaume Achaz, Amílcar Teixeira, Vincent Prié, L Filipe C Castro, Elsa Froufe

{"title":"The Crown Pearl V2: an improved genome assembly of the European freshwater pearl mussel Margaritifera margaritifera (Linnaeus, 1758).","authors":"André Gomes-Dos-Santos, Manuel Lopes-Lima, André M Machado, Thomas Forest, Guillaume Achaz, Amílcar Teixeira, Vincent Prié, L Filipe C Castro, Elsa Froufe","doi":"10.46471/gigabyte.81","DOIUrl":"10.46471/gigabyte.81","url":null,"abstract":"Contiguous assemblies are fundamental to deciphering the composition of extant genomes. In molluscs, this is considerably challenging owing to the large size of their genomes, heterozygosity, and widespread repetitive content. Consequently, long-read sequencing technologies are fundamental for high contiguity and quality. The first genome assembly of Margaritifera margaritifera (Linnaeus, 1758) (Mollusca: Bivalvia: Unionida), a culturally relevant, widespread, and highly threatened species of freshwater mussels, was recently generated. However, the resulting genome is highly fragmented since the assembly relied on short-read approaches. Here, an improved reference genome assembly was generated using a combination of PacBio CLR long reads and Illumina paired-end short reads. This genome assembly is 2.4 Gb long, organized into 1,700 scaffolds with a contig N50 length of 3.4 Mbp. The ab initio gene prediction resulted in 48,314 protein-coding genes. Our new assembly is a substantial improvement and an essential resource for studying this species' unique biological and evolutionary features, helping promote its conservation.","PeriodicalId":73157,"journal":{"name":"GigaByte (Hong Kong, China)","volume":"2023 ","pages":"gigabyte81"},"PeriodicalIF":0.0,"publicationDate":"2023-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10189783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9862369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycobacterial metabolic model development for drug target identification. 用于药物靶点鉴定的分枝杆菌代谢模型开发。

GigaByte (Hong Kong, China) Pub Date : 2023-04-30 eCollection Date: 2023-01-01 DOI: 10.46471/gigabyte.80

Bridget P Bannerman, Alexandru Oarga, Jorge Júlvez

{"title":"Mycobacterial metabolic model development for drug target identification.","authors":"Bridget P Bannerman, Alexandru Oarga, Jorge Júlvez","doi":"10.46471/gigabyte.80","DOIUrl":"10.46471/gigabyte.80","url":null,"abstract":"Antibiotic resistance is increasing at an alarming rate, and three related mycobacteria are sources of widespread infections in humans. According to the World Health Organization, Mycobacterium leprae, which causes leprosy, is still endemic in tropical countries; Mycobacterium tuberculosis is the second leading infectious killer worldwide after COVID-19; and Mycobacteroides abscessus, a group of non-tuberculous mycobacteria, causes lung infections and other healthcare-associated infections in humans. Due to the rise in resistance to common antibacterial drugs, it is critical that we develop alternatives to traditional treatment procedures. Furthermore, an understanding of the biochemical mechanisms underlying pathogenic evolution is important for the treatment and management of these diseases. In this study, metabolic models have been developed for two bacterial pathogens, M. leprae and My. abscessus, and a new computational tool has been used to identify potential drug targets, which are referred to as bottleneck reactions. The genes, reactions, and pathways in each of these organisms have been highlighted; the potential drug targets can be further explored as broad-spectrum antibacterials and the unique drug targets for each pathogen are significant for precision medicine initiatives. The models and associated datasets described in this paper are available in GigaDB, Biomodels, and PatMeDB repositories.","PeriodicalId":73157,"journal":{"name":"GigaByte (Hong Kong, China)","volume":"2023 ","pages":"gigabyte80"},"PeriodicalIF":0.0,"publicationDate":"2023-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10154535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9433054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The first genome assembly of the amphibian nematode parasite (Aplectana chamaeleonis). 两栖动物线虫寄生虫（Aplectana chamaeleonis）的首次基因组组装。

GigaByte (Hong Kong, China) Pub Date : 2023-03-27 eCollection Date: 2023-01-01 DOI: 10.46471/gigabyte.79

Lei Han, Tianlu Liu, Fengping He, Zhijun Hou

引用次数: 0

Genome assembly of the deep-sea coral Lophelia pertusa. 深海珊瑚 Lophelia pertusa 的基因组组装。

GigaByte (Hong Kong, China) Pub Date : 2023-03-16 eCollection Date: 2023-01-01 DOI: 10.46471/gigabyte.78

Santiago Herrera, Erik E Cordes

引用次数: 0

PhysiCOOL: A generalized framework for model Calibration and Optimization Of modeLing projects. PhysiCOOL：用于模型校准和优化模式化项目的通用框架。

GigaByte (Hong Kong, China) Pub Date : 2023-02-28 eCollection Date: 2023-01-01 DOI: 10.46471/gigabyte.77

Inês G Gonçalves, David A Hormuth, Sandhya Prabhakaran, Caleb M Phillips, José Manuel García-Aznar

{"title":"PhysiCOOL: A generalized framework for model Calibration and Optimization Of modeLing projects.","authors":"Inês G Gonçalves, David A Hormuth, Sandhya Prabhakaran, Caleb M Phillips, José Manuel García-Aznar","doi":"10.46471/gigabyte.77","DOIUrl":"10.46471/gigabyte.77","url":null,"abstract":"In silico models of biological systems are usually very complex and rely on a large number of parameters describing physical and biological properties that require validation. As such, parameter space exploration is an essential component of computational model development to fully characterize and validate simulation results. Experimental data may also be used to constrain parameter space (or enable model calibration) to enhance the biological relevance of model parameters. One widely used computational platform in the mathematical biology community is PhysiCell, which provides a standardized approach to agent-based models of biological phenomena at different time and spatial scales. Nonetheless, one limitation of PhysiCell is the lack of a generalized approach for parameter space exploration and calibration that can be run without high-performance computing access. Here, we present PhysiCOOL, an open-source Python library tailored to create standardized calibration and optimization routines for PhysiCell models.","PeriodicalId":73157,"journal":{"name":"GigaByte (Hong Kong, China)","volume":"2023 ","pages":"gigabyte77"},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10027115/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9164410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improvements to the Gulf pipefish Syngnathus scovelli genome. 对墨西哥湾河豚Syngnathus scovelli基因组的改进。

GigaByte (Hong Kong, China) Pub Date : 2023-02-20 eCollection Date: 2023-01-01 DOI: 10.46471/gigabyte.76

Balan Ramesh, Clay M Small, Hope Healey, Bernadette Johnson, Elyse Barker, Mark Currey, Susan Bassham, Megean Myers, William A Cresko, Adam Gregory Jones

{"title":"Improvements to the Gulf pipefish Syngnathus scovelli genome.","authors":"Balan Ramesh, Clay M Small, Hope Healey, Bernadette Johnson, Elyse Barker, Mark Currey, Susan Bassham, Megean Myers, William A Cresko, Adam Gregory Jones","doi":"10.46471/gigabyte.76","DOIUrl":"10.46471/gigabyte.76","url":null,"abstract":"The Gulf pipefish Syngnathus scovelli has emerged as an important species for studying sexual selection, development, and physiology. Comparative evolutionary genomics research involving fishes from Syngnathidae depends on having a high-quality genome assembly and annotation. However, the first S. scovelli genome assembled using short-read sequences and a smaller RNA-sequence dataset has limited contiguity and a relatively poor annotation. Here, using PacBio long-read high-fidelity sequences and a proximity ligation library, we generate an improved assembly to obtain 22 chromosome-level scaffolds. Compared to the first assembly, the gaps in the improved assembly are smaller, the N75 is larger, and our genome is ~95% BUSCO complete. Using a large body of RNA-Seq reads from different tissue types and NCBI's Eukaryotic Annotation Pipeline, we discovered 28,162 genes, of which 8,061 are non-coding genes. Our new genome assembly and annotation are tagged as a RefSeq genome by NCBI and provide enhanced resources for research work involving S. scovelli..","PeriodicalId":73157,"journal":{"name":"GigaByte (Hong Kong, China)","volume":"2023 ","pages":"gigabyte76"},"PeriodicalIF":0.0,"publicationDate":"2023-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10038202/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9561070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PhysiCOOL: A generalized framework for model Calibration and Optimization Of modeLing projects PhysiCOOL:modeLing项目模型校准和优化的通用框架

GigaByte (Hong Kong, China) Pub Date : 2023-02-13 DOI: 10.1101/2022.11.17.516671

Inês G. Gonçalves, D. Hormuth, Sandhya Prabhakaran, C. Phillips, J. García-Aznar

引用次数: 1

Nanopore-based enrichment of antimicrobial resistance genes - a case-based study. 基于纳米孔的抗菌药耐药性基因富集--一项基于案例的研究。

GigaByte (Hong Kong, China) Pub Date : 2023-01-25 eCollection Date: 2023-01-01 DOI: 10.46471/gigabyte.75

Adrian Viehweger, Mike Marquet, Martin Hölzer, Nadine Dietze, Mathias W Pletz, Christian Brandt

{"title":"Nanopore-based enrichment of antimicrobial resistance genes - a case-based study.","authors":"Adrian Viehweger, Mike Marquet, Martin Hölzer, Nadine Dietze, Mathias W Pletz, Christian Brandt","doi":"10.46471/gigabyte.75","DOIUrl":"10.46471/gigabyte.75","url":null,"abstract":"Rapid screening of hospital admissions to detect asymptomatic carriers of resistant bacteria can prevent pathogen outbreaks. However, the resulting isolates rarely have their genome sequenced due to cost constraints and long turn-around times to get and process the data, limiting their usefulness to the practitioner. Here we used real-time, on-device target enrichment (\"adaptive\") sequencing as a highly multiplexed assay covering 1,147 antimicrobial resistance genes. We compared its utility against standard and metagenomic sequencing, focusing on an isolate of Raoultella ornithinolytica harbouring three carbapenemases (NDM, KPC, VIM). Based on this experimental data, we then modelled the influence of several variables on the enrichment results and predicted the large effect of nucleotide identity (higher is better) and read length (shorter is better). Lastly, we showed how all relevant resistance genes are detected using adaptive sequencing on a miniature (\"Flongle\") flow cell, motivating its use in a clinical setting to monitor similar cases and their surroundings.","PeriodicalId":73157,"journal":{"name":"GigaByte (Hong Kong, China)","volume":"2023 ","pages":"gigabyte75"},"PeriodicalIF":0.0,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10027057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9534172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improvements to the Gulf pipefish Syngnathus scovelli genome 海湾管鱼(Syngnathus scovelli)基因组的改良

GigaByte (Hong Kong, China) Pub Date : 2023-01-20 DOI: 10.1101/2023.01.23.525209

B. Ramesh, CM Small, H. Healey, B. Johnson, E. Barker, M. Currey, S. Bassham, M. Myers, WA Cresko, Ag Jones

{"title":"Improvements to the Gulf pipefish Syngnathus scovelli genome","authors":"B. Ramesh, CM Small, H. Healey, B. Johnson, E. Barker, M. Currey, S. Bassham, M. Myers, WA Cresko, Ag Jones","doi":"10.1101/2023.01.23.525209","DOIUrl":"https://doi.org/10.1101/2023.01.23.525209","url":null,"abstract":"The Gulf pipefish Syngnathus scovelli has emerged as an important species in the study of sexual selection, development, and physiology, among other topics. The fish family Syngnathidae, which includes pipefishes, seahorses, and seadragons, has become an increasingly attractive target for comparative research in ecological and evolutionary genomics. These endeavors depend on having a high-quality genome assembly and annotation. However, the first version of the S. scovelli genome assembly was generated by short-read sequencing and annotated using a small set of RNA-sequence data, resulting in limited contiguity and a relatively poor annotation. Here, we present an improved genome assembly and an enhanced annotation, resulting in a new official gene set for S. scovelli. By using PacBio long-read high-fidelity (Hi-Fi) sequences and a proximity ligation (Hi-C) library, we fill small gaps and join the contigs to obtain 22 chromosome-level scaffolds. Compared to the previously published genome, the gaps in our novel genome assembly are smaller, the N75 is much larger (13.3 Mb), and this new genome is around 95% BUSCO complete. The precision of the gene models in the NCBI’s eukaryotic annotation pipeline was enhanced by using a large body of RNA-Seq reads from different tissue types, leading to the discovery of 28,162 genes, of which 8,061 were non-coding genes. This new genome assembly and the annotation are tagged as a RefSeq genome by NCBI and thus provide substantially enhanced genomic resources for future research involving S. scovelli.","PeriodicalId":73157,"journal":{"name":"GigaByte (Hong Kong, China)","volume":"2023 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42096485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Distribution of mosquitoes (Diptera: Culicidae) in Thailand: a dataset. 泰国蚊类分布(双翅目:库蚊科):a dataset。

GigaByte (Hong Kong, China) Pub Date : 2023-01-01 DOI: 10.46471/gigabyte.90

Chutipong Sukkanon, Wannapa Suwonkerd, Kanutcharee Thanispong, Manop Saeung, Pairpailin Jhaiaun, Suntorn Pimnon, Kanaphot Thongkhao, Sylvie Manguin, Theeraphap Chareonviriyaphap

{"title":"Distribution of mosquitoes (Diptera: Culicidae) in Thailand: a dataset.","authors":"Chutipong Sukkanon, Wannapa Suwonkerd, Kanutcharee Thanispong, Manop Saeung, Pairpailin Jhaiaun, Suntorn Pimnon, Kanaphot Thongkhao, Sylvie Manguin, Theeraphap Chareonviriyaphap","doi":"10.46471/gigabyte.90","DOIUrl":"https://doi.org/10.46471/gigabyte.90","url":null,"abstract":"Mosquitoes play a crucial role as primary vectors for various infectious diseases in Thailand. Therefore, accurate distribution information is vital for effectively combating and better controlling mosquito-borne diseases. Here, we present a curated dataset of the mosquito distribution in Thailand comprising 12,278 records of at least 117 mosquito species (Diptera: Culicidae). The main genera included in the dataset are Aedes, Anopheles, Armigeres, Culex, and Mansonia. From 2007 to 2023, data were collected through routine mosquito surveillance and research projects from 1,725 locations across 66 (out of 77) Thai provinces. The majority of the data were extracted from a Thai database of the Thailand Malaria Elimination Program. To facilitate broader access to mosquito-related data and support further exploration of the Thai mosquito fauna, the data were translated into English. Our dataset has been published in the Global Biodiversity Information Facility, making it available for researchers worldwide.","PeriodicalId":73157,"journal":{"name":"GigaByte (Hong Kong, China)","volume":"2023 ","pages":"gigabyte90"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10498097/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10270936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0