Frontiers in reproductive health最新文献

{"title":"Editorial: Participant characteristics and biological markers for endometriosis diagnosis or prediction of treatment response.","authors":"Amy L Shafrir, Philippa T K Saunders","doi":"10.3389/frph.2024.1487745","DOIUrl":"10.3389/frph.2024.1487745","url":null,"abstract":"","PeriodicalId":73103,"journal":{"name":"Frontiers in reproductive health","volume":"6 ","pages":"1487745"},"PeriodicalIF":2.3,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11693682/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The magnitude of casual sex and associated factors among students at Debre Berhan University.","authors":"Tsega Mathewos, Esubalew Tesfahun, Muluken Tessema Aemiro, Tadesse Mamo Dejene","doi":"10.3389/frph.2024.1491617","DOIUrl":"10.3389/frph.2024.1491617","url":null,"abstract":"<p><strong>Background: </strong>Today's youth are adopting a new trend: casual sex. College students are expected to engage in a range of potentially harmful sexual activities, such as casual sex. Numerous factors can lead students to engage in casual sexual encounters. However, there has been limited research on the prevalence of casual sex and related issues in higher education institutions in Ethiopia.</p><p><strong>Objective: </strong>To assess the prevalence of casual sex and its associated factors among regular undergraduate students at Debre Berhan University in 2023.</p><p><strong>Methods: </strong>From 15 May 2023, to 30 May 2023, regular undergraduate students from Debre Berhan University participated in a cross-sectional study. A total of 512 samples were collected using a multistage sampling technique. Subsequently, a simple random sampling technique was employed to select the students, with a proportionate allocation of samples distributed to randomly selected colleges, departments, and academic years. The data were collected using a structured, pretested, and self-administered questionnaire. Once the data were imported into Epi-data version 4.6 and exported to SPSS version 20 for analysis, frequencies, percentages, and tables were generated. Bivariate and multivariate logistic regression models were used to identify characteristics associated with casual sex.</p><p><strong>Results: </strong>The prevalence of casual sex among regular undergraduate students at Debre Berhan University was 31.0% 95% CI (27.2-34.6). Several factors were significantly associated with casual sex. These included students from urban areas [AOR: 2.95; 95% CI (1.66-5.24)], students with poor academic performance [AOR: 2.81; 95% CI (1.51-5.22)], alcohol consumption [AOR: 4.59; 95% CI (2.60-8.12)], attending nightclubs [AOR: 2.75; 95% CI (1.47-5.16)], watching pornography [AOR: 2.59; 95% CI (1.47-5.16)], and peer pressure [AOR: 2.24; 95% CI (1.38-3.65)].</p><p><strong>Conclusion: </strong>The prevalence of casual sex was high among Debre Berhan University students. In order to lessen the negative effects of casual sex practices, this study found predictors that can be avoided through various interventions. Key preventative measures include improving student academic performance, providing engaging and free entertainment, restricting access to pornographic websites on university Wi-Fi, and employing peer educators.</p>","PeriodicalId":73103,"journal":{"name":"Frontiers in reproductive health","volume":"6 ","pages":"1491617"},"PeriodicalIF":2.3,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11693712/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Attitudes and practices regarding contraception among male students in a Nigerian tertiary educational institution: a cross-sectional study.","authors":"Awawu G Nmadu, Jeremiah Musa, Istifanus A Joshua, Adegboyega M Oyefabi, Nafisat O Usman, Bilkisu Nwankwo, Tukur Dahiru","doi":"10.3389/frph.2024.1439900","DOIUrl":"10.3389/frph.2024.1439900","url":null,"abstract":"<p><strong>Objectives: </strong>Limited data exists on attitudes and practices of young men in tertiary institutions towards contraception. This study assesses attitudes and practices regarding contraception among male students in a tertiary educational institution in northwestern Nigeria, identifying factors influencing these attitudes.</p><p><strong>Methods: </strong>This cross-sectional study conducted from July to August 2021 at Kaduna Polytechnic, Nigeria, involved 160 male students chosen via multistage sampling. Structured questionnaires gathered information on socio-demographic characteristics, awareness, attitudes, and contraceptive practices. Analysis utilized SPSS version 23.0, employing descriptive statistics, bivariate, and multivariable logistic regression analyses to determine significant factors influencing contraception attitudes.</p><p><strong>Results: </strong>Findings revealed a mean respondent age of 25.4 ± 3.5 years, with most being single (71.3%) and 51.2% sexually active. While awareness of contraceptives was high (85.6%), almost half (46.7%) exhibited negative attitudes towards contraception. Common concerns included reliability, impact on sexual pleasure, and traditional gender norms. Only 35.8% had ever used contraception, primarily using withdrawal and male condoms. Significant factors influencing positive attitudes included being aged 18-24 years compared to 26-35 years (AOR = 2.66, 95% CI: 1.22-5.82).</p><p><strong>Conclusion: </strong>Culturally sensitive interventions are vital for improving negative attitudes and low contraceptive use among male Nigerian youth.</p>","PeriodicalId":73103,"journal":{"name":"Frontiers in reproductive health","volume":"6 ","pages":"1439900"},"PeriodicalIF":2.3,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11693658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Provider views on vasectomy: cultural, gender, and political elements of Men's decisions to seek publicly funded services.","authors":"Michelle Teti, Denise Raybon, Stephanie Spitz, Shelby Webb, Jacki Witt, Kristin Metcalf-Wilson","doi":"10.3389/frph.2024.1386244","DOIUrl":"10.3389/frph.2024.1386244","url":null,"abstract":"<p><strong>Introduction: </strong>Evidence suggests a new demand for vasectomies following the Supreme Court's Dobbs v. Jackson Women's Health Organization [597 U.S. 215 (2022), (Dobbs)]. Vasectomies are safer and more cost-effective than tubal sterilization. Understanding how to support men's use of this procedure is important to improving sexual and reproductive health and wellbeing (SRHW). This study is an exploration of health care providers' perspectives on the gender, cultural and political influences on vasectomies at Title X-funded clinics across all regions of the US.</p><p><strong>Methods: </strong>Providers or health services staff (<i>N</i> = 21) at Title X-funded settings currently offering vasectomy services in their own clinics or via referral were recruited using list serve and email outreach. Participants took part in one-on-one or small group interviews about vasectomy procedures, patient experiences, and trends. A thematic analysis of interview transcripts through an iterative process of reviewing, note-taking, and discussing data assessed provider views on patient cultural, gender, and political vasectomy influences.</p><p><strong>Results: </strong>Qualitative interviews yielded four themes related to participant access to services, including income challenges, language barriers, medical distrust, and societal gender roles that stressed women's responsibility for contraception. Two additional themes focused on men's fear of losing reproductive health options and desire to \"step up\" to do their part to prevent unplanned pregnancies.</p><p><strong>Discussion: </strong>Interviewees stressed that vasectomy was for everyone but identified sub-groups of men who still faced logistical and social access challenges to the procedure. Providers also believed that men were concerned over reproductive justice in the US and wanted to do their part to help prevent unplanned pregnancies. They thought that the Dobbs decision may mark a turning point in reproductive care that could ultimately better public health initiatives and overall SRHW by including men in the conversation. Vasectomy education, marketing-along with policy changes that ease access, can support this goal.</p>","PeriodicalId":73103,"journal":{"name":"Frontiers in reproductive health","volume":"6 ","pages":"1386244"},"PeriodicalIF":2.3,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11688344/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proposals for integrated public management of the menopausal transition and postmenopause through Spanish women's experiences: a qualitative study.","authors":"Clara Selva","doi":"10.3389/frph.2024.1483267","DOIUrl":"10.3389/frph.2024.1483267","url":null,"abstract":"<p><strong>Introduction: </strong>In Spain, legislation protecting women during the menopausal transition and postmenopause is still in its early stages. For public policies to be effective, it is essential that their design, implementation, and evaluation actively involve women going through this stage. Only from their experience and perspective can the impact of measures be maximized and ensure that they truly respond to their needs and realities. The goal of this article is to identify and analyze proposals for action that public organizations in Spain could undertake to improve the management of and transition through menopause. On the basis of the initiatives identified through women's narratives, this study aims to establish a solid basis for the inclusion of future governmental policies and practices in Spain. Adapting to the economic conditions and sociocultural aspects specific to each region can stimulate reflection and debate on their policies regarding menopause.</p><p><strong>Methods: </strong>This qualitative study, which was conducted in Spain, was based on 20 semistructured interviews with women who had experienced physiological and symptomatic menopause in the last five years, were postmenopausal, and were actively employed. The interviews were audio-recorded, transcribed, and analyzed following an inductive thematic analysis.</p><p><strong>Results: </strong>The stories of the interviewees indicate three categories of government action comprising a set of specific measures. More specifically, the categories relate to: (a) proposals for accompaniment and social awareness, (b) educational and training proposals, and (c) political and regulatory proposals.</p><p><strong>Conclusions: </strong>This study identifies governmental proposals and specific actions to improve support for the menopausal transition and postmenopause in Spain. While its findings may be applicable to other countries, further research is needed to explore how they could be adapted to the different socioeconomic and cultural realities of those contexts. Ultimately, the proposals presented lay the groundwork for developing public policies and laws that respond to the needs of women, improving their well-being and promoting gender equity in public health.</p>","PeriodicalId":73103,"journal":{"name":"Frontiers in reproductive health","volume":"6 ","pages":"1483267"},"PeriodicalIF":2.3,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11683108/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends and projections of the burden of disease for male infertility in China from 1990 to 2021: an analysis from the Global Burden of Disease 2021 study.","authors":"Ran Xu, Xin-Jun Wang, Qing-Cheng Lin, Yan-Ting Zhuang, Qing-Ying Zhou, Nai-Fen Xu, Ding-Qin Zheng","doi":"10.3389/frph.2024.1501675","DOIUrl":"10.3389/frph.2024.1501675","url":null,"abstract":"<p><strong>Background: </strong>Currently, male infertility represents a serious disease burden worldwide, and China is one of the most affected countries. The aim of this study was to examine the evolution of the disease burden of male infertility in China during the period 1990-2021 and to project the trend for 2022-2036.</p><p><strong>Methods: </strong>By screening and processing data from the Global Burden of Disease 2021, this study obtained data on the prevalence, disability-adjusted life years and corresponding rates and age-standardised rates of male infertility between 1990 and 2021. To assess the trend in the burden of male infertility over the past 30 years, the annual percentage change and the average annual percentage change were calculated from the above data using Joinpoint regression models. In addition, age-period-cohort models were used to estimate the independent effects of age, period and cohort factors on male infertility, and Bayesian projection models were used to predict the trend in the disease over the next 15 years.</p><p><strong>Result: </strong>In both 1990 and 2021, the burden of male infertility tended to increase and then decrease with age, with the heaviest burden in the 35-39 age group. Meanwhile, the Joinpoint model found statistically significant average annual percentage changes in age-standardised prevalence and age-standardised disability-adjusted life years of 0.14% and 0.19%, respectively. In addition, the trend for both was a gradual increase over time until 1994 and a gradual decrease over time after 1994. In the age-period-cohort analysis model, age, period and cohort effects indicated that 35-39 years, 1997-2001 and 1945-1949 were the years with the highest risk of male infertility. Finally, the Bayesian projection model suggested that the disease burden of male infertility in China would show a decreasing trend over the next 15 years.</p><p><strong>Conclusion: </strong>From 1990 to 2021, the disease burden of male infertility in China generally increased. However, thanks to a number of factors, including China's proactive health policies and effective management, the burden of disease has continued to decrease in the last decade and is projected to continue declining from 2022 to 2036. To sustain this positive trend, it remains essential for China to maintain and strengthen effective management and control of male infertility.</p>","PeriodicalId":73103,"journal":{"name":"Frontiers in reproductive health","volume":"6 ","pages":"1501675"},"PeriodicalIF":2.3,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11683063/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The factors associated with teenage pregnancy among young women aged between 15 and 19 years in Rwanda: a retrospective cross-sectional study on the Rwanda Demographic Health Survey 2019-2020.","authors":"Felix Nduhuye, Emmanuel Kubana, Stella Matutina, David Mwesigye, Athanase Munyaneza, Laetitia Nyirazinyoye","doi":"10.3389/frph.2024.1453933","DOIUrl":"10.3389/frph.2024.1453933","url":null,"abstract":"<p><strong>Background: </strong>Teenage pregnancy is a significant public health issue and is strongly associated with risky sexual behaviors such as early sexual initiation, unprotected sex, and multiple sexual partners. According to the 2014 World Health Organization report, 11% of all births worldwide were to teenagers aged 15-19 years, with more than 95% of these pregnancies occurring in low- and middle-income countries, particularly in sub-Saharan Africa, which bears much of this burden. In Rwanda, the prevalence of teenage pregnancy has risen from 4.1% in 2005 to 7.3% in 2014, indicating a growing concern. However, there is limited and inconsistent evidence on the factors contributing to teenage pregnancy. Hence, our study aimed to investigate the factors associated with teenage pregnancy. This research seeks to provide valuable insights for targeted interventions, which are urgently needed in light of the increasing rates.</p><p><strong>Methods: </strong>We employed a cross-sectional study design, utilizing data from the 2019/2020 Rwanda Demographic Health Survey of 3,258 eligible participants aged 15-19 years. To identify factors associated with teenage pregnancy, we performed a bivariate logistic regression analysis. The significant variables from the bivariate analysis were then exported into multivariate logistic regression models, with the results presented as odds ratios (ORs) along with 95% confidence intervals (CIs) and a significance threshold set at 5%.</p><p><strong>Results: </strong>Our findings indicated that teenagers aged 18-19 years were more likely to experience pregnancy compared to those younger than 17 (OR = 4.2; 95% CI: 2.16-8.37). Adolescents who had engaged in sexual activity 95 times or more had a significantly higher likelihood of becoming pregnant than those with less frequent sexual activity (OR = 13.53; 95% CI: 5.21-35.12). Furthermore, adolescents with parents with a secondary education were 80% less likely to become pregnant compared to those with parents with a primary or no education (OR = 0.2; 95% CI: 0.07-0.63).</p><p><strong>Conclusion: </strong>Our study revealed that teenage pregnancy is shaped by several individual factors including age and sexual behavior, along with parental education levels. These findings underscore the critical need for targeted sexual education and enhanced family support systems to mitigate teenage pregnancies. Further, longitudinal studies are essential for establishing causality and guiding effective policy development.</p>","PeriodicalId":73103,"journal":{"name":"Frontiers in reproductive health","volume":"6 ","pages":"1453933"},"PeriodicalIF":2.3,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11671394/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142904152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disparities in PrEP use and unmet need across PEPFAR-supported programs: doubling down on prevention to put people first and end AIDS as a public health threat by 2030.","authors":"Trena I Mukherjee, Mitchell Yep, Megan Koluch, Sisay Alemayehu Abayneh, Gizachew Eyassu, Elizabeth Manfredini, Sara Herbst","doi":"10.3389/frph.2024.1488970","DOIUrl":"10.3389/frph.2024.1488970","url":null,"abstract":"<p><strong>Background: </strong>In 2023, an estimated 1.3 million people newly acquired HIV. In the same year, 3.5 million individuals received pre-exposure prophylaxis (PrEP), falling short of the UNAIDS target of 21.2 million by 2025. With over 90% of global PrEP programming supported by PEPFAR, a better understanding of disparities in PrEP provision is needed to inform PEPFAR's approach to reach and deliver prevention services and achieve UNAIDS 95-95-95 goals in all populations by 2025. The objective of this paper is to assess unmet PrEP need in PEPFAR-supported countries.</p><p><strong>Methods: </strong>We analyzed FY2023 Monitoring, Evaluation, and Reporting (MER) results from 48 PEPFAR-supported countries to calculate PrEP-to-need ratios (PnR) by geography and population. PnR offers an ecological measure to identify disparities and missed opportunities for PrEP programming. PnR was calculated as the ratio of PrEP users to the number of positive HIV tests. PrEP users are defined through new initiations (PrEP_NEW) and re-initiations or continuation (PrEP_CT). HTS_TST_POS measures the number of positive HIV tests and was used as a proxy for new diagnoses. PnR was also calculated using Naomi-estimated 2023 HIV incidence, where available. A higher PnR indicates more PrEP users relative to PrEP need in a population.</p><p><strong>Results: </strong>In FY23, 1,760,888 people initiated PrEP, and 1,736,144 people tested positive for HIV. PnR ranged from 0.12 (India) to 6.46 (Brazil), and 19 (40%) countries had fewer PrEP users than positive HIV tests (PnR <1.0). By population, people 15-24 years old, people who inject drugs, and transgender populations had the highest median PnR. When examining estimated HIV incidence, Mozambique and South Africa reported lower than average PnR and higher than average HIV incidence.</p><p><strong>Conclusion: </strong>PrEP use relative to population need varied greatly by country and subpopulation across PEPFAR programs, suggesting a need for greater advocacy, inclusivity, accessibility, and integrated prevention programming. PnR may be a useful indicator of population PrEP coverage and unmet need, and can inform effective, data-driven, and person-centered PEPFAR prevention programming and policies. Tailoring PrEP scale-up strategies by age, sex, key population, and geography is crucial to achieving UNAIDS targets and ending the AIDS epidemic as a public health threat for all by 2030.</p>","PeriodicalId":73103,"journal":{"name":"Frontiers in reproductive health","volume":"6 ","pages":"1488970"},"PeriodicalIF":2.3,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11671492/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142904148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knowledge, perceptions, and management of symptoms of hormonal imbalance among adolescent girls in selected schools in Ghana: a qualitative exploratory study.","authors":"Sawudatu Zakariah-Akoto, Benjamin Abuaku, Godfred Egbi, Bismark Edem Kofi Klu, Eric Kyei-Baafour, Michael Fokuo Ofori, Collins Stephen Ahorlu, Dorothy Yeboah-Manu","doi":"10.3389/frph.2024.1502352","DOIUrl":"10.3389/frph.2024.1502352","url":null,"abstract":"<p><strong>Introduction: </strong>Adolescent girls are more sensitive to hormonal imbalance with major impact on their nutritional, reproductive, physical, psychosocial, and academic wellbeing. This study explored adolescent girls' knowledge and perceptions of causes and management of symptoms of hormonal imbalance.</p><p><strong>Materials and methods: </strong>Using a qualitative approach, focus group discussions were conducted with 116 assented in-school adolescent girls aged 10-19 years between 3rd and 19th October 2022. Two urban and two rural communities in two regions were purposively selected for the study. Using a convenient sampling approach, participants were recruited from Upper Primary, Junior, and Senior High schools. Data was analyzed thematically using both inductive and deductive approaches.</p><p><strong>Results: </strong>Senior High School participants had a fair understanding of hormonal imbalance in both regions. Symptoms were perceived to include headaches, heavy and painful periods, and pimples, most of which participants alluded to experiencing. Perceived causes included natural process of adolescent growth, consumption of unhealthy diets, use of cosmetics and medications. Perceived reproductive effects included infertility, pregnancy disorders, breastfeeding challenges, and low sexual drive. Physiological effects included extreme fatigue, loss of appetite and impaired growth. Psychosocial and academic effects included mood swings/irritability, low self-esteem, poor inter-personal relationship, poor concentration in class and irregular school attendance. Female relatives and friends were mostly consulted for advice. Self-medication, dietary modification, physical activities, and personal hygiene were adopted to manage perceived symptoms. Self-medication was common, but physical activities and dietary modification were also adopted by most participants to manage symptoms.</p><p><strong>Conclusion: </strong>It is recommended that Ghana Education Service should formally incorporate hormonal-related issues into its School Health Education Programmes to enhance knowledge, attitudes, and management among adolescent girls at all levels of education.</p>","PeriodicalId":73103,"journal":{"name":"Frontiers in reproductive health","volume":"6 ","pages":"1502352"},"PeriodicalIF":2.3,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11671398/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142904150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial dysfunction signatures in idiopathic primary male infertility: a validated proteomics-based diagnostic approach.","authors":"Raneen Sawaid Kaiyal, Sromona D Mukherjee, Manesh Kumar Panner Selvam, Aaron W Miller, Sarah C Vij, Scott D Lundy","doi":"10.3389/frph.2024.1479568","DOIUrl":"10.3389/frph.2024.1479568","url":null,"abstract":"<p><strong>Research question: </strong>Male infertility accounts for almost half of all infertility cases worldwide, with idiopathic male infertility accounting for up to 30% of the cases. Sperm proteomics has revealed critical molecular pathway changes in men with infertility. However, the sperm mitochondrial proteome remains poorly understood. We attempted to answer the following question: Do patients with idiopathic primary male infertility exhibit a proteomic signature associated with mitochondrial dysfunction that could be used as a target for future mechanistic investigations?</p><p><strong>Design: </strong>Patients with idiopathic primary infertility (20-40 years old) referred to the Cleveland Clinic between March 2012 and April 2014 were compared with fertile donor controls. Sperm proteins were analyzed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis page (SDS-PAGE) and liquid chromatography-mass spectrometry (LC-MS), and differentially expressed proteins (DEPs) were identified based on significance test results and fold change thresholds. Protein expression was validated using western blotting.</p><p><strong>Results: </strong>Proteomic analysis of pooled samples from fertile donors (<i>n</i> = 5) and patients with idiopathic primary infertility (<i>n</i> = 5) identified 1,134 proteins, including 344 DEPs. Mitochondrial dysfunction topped the ingenuity toxicity list. Analysis of expression levels of three mitochondrial proteins known to combat oxidative stress revealed that peroxiredoxin-5 (PRDX5) and superoxide dismutase 2 (SOD2), but not glutathione disulphide reductase, were significantly decreased in patient samples compared with those in fertile-donor samples.</p><p><strong>Conclusions: </strong>This study revealed an association of downregulated expression of PRDX5 and SOD2 in sperm samples of patients with idiopathic primary male infertility. Our results support future mechanistic studies and development of advanced diagnostic methods to better identify men with mitochondria-related male infertility.</p>","PeriodicalId":73103,"journal":{"name":"Frontiers in reproductive health","volume":"6 ","pages":"1479568"},"PeriodicalIF":2.3,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11669654/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142899437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}