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Acta biochimica et biophysica; Academiae Scientiarum Hungaricae最新文献

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Vanadate inhibition of Na+K+. ATPase and K+-dependent p-nitrophenylphosphatase: a kinetic analysis. 钒酸盐对Na+K+的抑制作用。atp酶和K+依赖性对硝基苯基磷酸酶:动力学分析。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1983-01-01
A Blázovics, L Vodnyánszky, J Somogyi, I Horváth
{"title":"Vanadate inhibition of Na+K+. ATPase and K+-dependent p-nitrophenylphosphatase: a kinetic analysis.","authors":"A Blázovics,&nbsp;L Vodnyánszky,&nbsp;J Somogyi,&nbsp;I Horváth","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Vanadate in redox state +5 inhibited the Na+K+-activated ATPase as well as the potassium-stimulated p-nitrophenylphosphatase (p-NPPase) activities of plasma membrane fragments prepared from rat brain. Vanadate exhibited a mixed type inhibition on the Na+K+-ATP-ase activity. The same type of inhibition was observed when the p-NPPase activity of the enzyme preparation was measured either in the presence of 20 mM K+ or with 5 mM Na+ + 1 mM K+. When the reaction mixture contained 50 microM ATP, 5 mM Na+ and 1 mM K+, inhibition of p-NPP hydrolised by vanadate displayed a noncompetitive character. Higher noradrenalin concentration was required for counteracting, the inhibition of p-NPPase by vanadate in the presence of ATP than in its absence.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17392210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The importance of changes in ATP and ADP concentrations in the development of the phasic superprecipitation of actomyosin. ATP和ADP浓度变化在肌动球蛋白相超沉淀发展中的重要性。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1983-01-01
S Csabina, J Csongor, L Kónya, A Szöör
{"title":"The importance of changes in ATP and ADP concentrations in the development of the phasic superprecipitation of actomyosin.","authors":"S Csabina,&nbsp;J Csongor,&nbsp;L Kónya,&nbsp;A Szöör","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It has been shown previously that at physiological K+-concentrations the development of superprecipitation has a phasic character. Studying the reason of this effect, by the use of beta-32P-ADP ATP-regeneration from ADP was detected during the first phase of superprecipitation. Myokinase activity of actomyosin may be responsible for the appearance of this first phase of turbidity change of actomyosin suspension. Myokinase contributes to the stabilization of an ATP/ADP ratio within narrow limits. During the first phase of superprecipitation the ATP/ADP ratio is between these limits, while during the second phase the ratio has a low value. This phasic evolution of superprecipitation is, probably connected with the physiological functional state of the muscle (contraction or rigour).</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17731912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enzyme-enzyme interactions within human erythrocytes as suggested from prelytic release. 酶-酶在人红细胞内的相互作用，从预溶释放提示。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1983-01-01
E Cseke, G Szabolcsi
{"title":"Enzyme-enzyme interactions within human erythrocytes as suggested from prelytic release.","authors":"E Cseke,&nbsp;G Szabolcsi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The molecular sieving property of human erythrocyte membrane in slightly hypotonic media has been utilized for studying the intracellular localization of some proteins (Cseke et al., 1978, FEBS Lett. 96 15-18). It is now shown that four proteins which appear to be uniformly distributed within the cell behave similarly irrespective of the membrane resistance differences caused by changes of temperature and metabolic energy supply. Five enzymes catalyzing consecutive reactions in the glycolytic pathway between triosephosphate formation and lactate production are released from erythrocytes in quantities deviating from those predicted on the basis of molecular sieving. The data are compatible with the assumption that these enzymes form complexes with each other under in vivo conditions and that complex formation is facilitated if glycolysis is working at high rate (37 degrees C).</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17489026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microwave method for determining dielectric parameters of living biological objects. III. Study of water binding in frog nerve. 测定活生物物体介电参数的微波方法。3青蛙神经中水结合的研究。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1983-01-01
S Misik, G Masszi
{"title":"Microwave method for determining dielectric parameters of living biological objects. III. Study of water binding in frog nerve.","authors":"S Misik,&nbsp;G Masszi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Dielectric constant and loss of frog nerve was determined at 6.3 GHz frequency and 20-50 degrees C temperature by a microwave cavity resonator measuring method developed by us. As a result of temperature denaturation the dielectric constant of frog nerve decreases, while its dielectric loss increases. According to our measurements the volume of bound water in nerve is approximately 0.44 ml/l ml dry material. The activation energy of water in the nerve is 16.8 kJ/mol.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17666707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assembly and reliability of an X-ray microanalyser system with a possibility for independent mass measurement. 具有独立质量测量可能性的x射线微分析仪系统的装配和可靠性。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1983-01-01
L Siklós
{"title":"Assembly and reliability of an X-ray microanalyser system with a possibility for independent mass measurement.","authors":"L Siklós","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A new X-ray microanalyser assembly is described, the main parts of which are a JEOL 100 B transmission electron microscope, a JEOL ASID I scanning attachment, an EDAX 183 B semiconductor detector and a KFKI ICA 70 4k multichannel analyser. By using this equipment, qualitative analysis, equivalent to the original EDAX version, can be performed. Furthermore it is possible to visualize simultaneously the spatial distribution of two or more elements in the sample. The mass of the excited volume of the sample can be determined, independently of the X-ray measurements, by using the electron detector of the scanning attachment. The reproducibility of such measurements is demonstrated and a calibration curve is given. By recording the excited mass of the sample during the X-ray analysis, the damage caused by the exciting electron beam was also examined.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17731911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The present state of the chemiosmotic coupling theory. 化学渗透耦合理论的现状。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1983-01-01
H V Westerhoff, S L Helgerson, S M Theg, O van Kooten, M Wikström, V P Skulachev, Z Dancsházy
{"title":"The present state of the chemiosmotic coupling theory.","authors":"H V Westerhoff,&nbsp;S L Helgerson,&nbsp;S M Theg,&nbsp;O van Kooten,&nbsp;M Wikström,&nbsp;V P Skulachev,&nbsp;Z Dancsházy","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Although the general principles of the chemiosmotic coupling theory have become widely accepted, the (degree of) loc(aliz)ation of electrochemical proton potential difference cannot yet be deduced from the existing experimental data. Many results are not in ready accordance with the idea that one protonic electrochemical potential difference, i.e. the one between a homogeneous inner and a homogeneous outer aqueous phase, would be the high-free-energy intermediate of membrane-linked free-energy transduction. Rather, free-energy transduction in an organelle like a mitochondrion or a chloroplast might take place in large number (about 1 per H+-ATPase) of miniature chemiosmotic systems. The energized protons produced in such a miniature system might be largely (but not totally) confined to a proton-domain belonging to it. Hence, there might be many (rather than one) different relevant proton gradients.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17297168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Abstracts: 22nd meeting of the Hungarian Biochemical Society. 25-28 August, 1982, Debrecen. 摘要:第22届匈牙利生化学会会议。1982年8月25-28日，德布勒森。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1982-01-01
{"title":"Abstracts: 22nd meeting of the Hungarian Biochemical Society. 25-28 August, 1982, Debrecen.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18129858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Homologous partial sequences in dehydrogenases. 脱氢酶的同源部分序列。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1981-04-01 DOI: 10.1042/bst009264pb
G. Mátrai, F. Darvas, T. Keleti
{"title":"Homologous partial sequences in dehydrogenases.","authors":"G. Mátrai, F. Darvas, T. Keleti","doi":"10.1042/bst009264pb","DOIUrl":"https://doi.org/10.1042/bst009264pb","url":null,"abstract":"Such details of the primary structure were sought that are common in all dehydrogenases of known amino acid sequence. Twenty-six sequences of eight kinds of dehydrogenase (D-glyceraldehyde-3-phosphate dehydrogenase, alcohol dehydrogenase, lactate dehydrogenase, glutamate dehydrogenase, glycerol-3-phosphate dehydrogenase, ribitol dehydrogenase, L-hydroxyacyl-CoA dehydrogenase and homoserine dehydrogenase) have been compared by the aid of the artificial intelligence language Prolog, the amino acids being classified into groups according to their chemical properties, and alpha-helix or beta-sheet-forming abilities. We found tetrapeptides that occurred in all dehydrogenases examined. By using these tetrapeptides as markers a population of 84 partial sequences has been described. The partial sequences constituting this population are peptides comprising 35 residues. It has been shown statistically that these peptides form a homogeneous sample as regards the frequency of occurrence of amino acid groups. This statistically homogeneous partial sequences can be regarded as homologous and it is assumed that their presence is characteristic of dehydrogenases.","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1981-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88214989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Steroid spectrum in human urine as revealed by gas chromatography. IV. Changes in the exception of 16-oxygenated neutral steroids by children with 21-hydroxylase deficiency during different stages of development. 气相色谱法测定人尿液中的类固醇光谱。4 . 21-羟化酶缺乏症儿童在不同发育阶段16-氧合中性类固醇异常的变化。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1981-01-01
L Kecskés, Z Juricskay, G Kosztoláni, M Szécsényi
{"title":"Steroid spectrum in human urine as revealed by gas chromatography. IV. Changes in the exception of 16-oxygenated neutral steroids by children with 21-hydroxylase deficiency during different stages of development.","authors":"L Kecskés,&nbsp;Z Juricskay,&nbsp;G Kosztoláni,&nbsp;M Szécsényi","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17340478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The purification of polynucleotide phosphorylase from Thermus aquaticus by the use of heparin-sepharose 4B affinity chromatography. 采用肝素-sepharose 4B亲和层析纯化水热菌多核苷酸磷酸化酶。
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae Pub Date : 1981-01-01
P I Bauer, K G Büki
{"title":"The purification of polynucleotide phosphorylase from Thermus aquaticus by the use of heparin-sepharose 4B affinity chromatography.","authors":"P I Bauer,&nbsp;K G Büki","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The polynucleotide phosphorylase of Thermus aquaticus was purified using ammonium sulfate fractionation and column chromatography on DEAE-cellulose, heparin-Sepharose 4B and DEAE-Sephadex A25. The enzyme was purified 1500-fold and was 90-95% homogeneous as checked by polyacrylamide gel electrophoresis. It has a molecular weight of 275 000 and consists of four identical subunits. The Km values for the enzyme as determined in polymerization (ADP, GDP, UDP) and phosphorolytic reactions (poly A, poly U) are in the same concentration range as in the case of the enzyme deriving from mesophilic microorganisms. Furthermore, the enzyme is primer dependent and its activity is lost gradually at temperatures higher than 65 degrees C. In the base ratio of the copolymers followed the input base ratio polymerization reactions with polyUA, while with polyAG and polyUG a marked difference between the initial base ratio and the base composition of copolymers was observed.</p>","PeriodicalId":7308,"journal":{"name":"Acta biochimica et biophysica; Academiae Scientiarum Hungaricae","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18359785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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