Fish and shellfish immunology reports最新文献

{"title":"Dietary supplementation of Polygonum chinense improves the immunity of Asian seabass, Lates calcarifer (Bloch, 1790) against Vibrio harveyi infection","authors":"Abdul Razak Rashidah ,&nbsp;Mohamed Shariff ,&nbsp;Fatimah Md. Yusoff ,&nbsp;Intan Safinar Ismail","doi":"10.1016/j.fsirep.2023.100118","DOIUrl":"https://doi.org/10.1016/j.fsirep.2023.100118","url":null,"abstract":"<div><p>Aquaculture plays a significant role in the overall fish production in Malaysia, contributing a substantial quantity of food-fish amounting to roughly 573,683 tonnes with an estimated economic value of US$860 million in 2022. However, diseases have become a significant limitation for aquaculture production. Therefore, herbal immunostimulant has been considered a natural and practical approach of preventing disease infection in fish. The ability of <em>Polygonum chinense</em> extract (PCE) on haemato-biochemistry parameters, immunomodulatory properties, and disease resistance of <em>Lates calcarifer</em> (Asian seabass) under <em>Vibrio harveyi</em> challenge was evaluated in this study, with a focus on dose-response associations and variability over various exposure durations (0-, 7- and 14-day post-infection). A total of 480 Asian seabass (9.5 ± 0.2 g) were distributed in 12 aquaria and fed four diets supplemented with 0 (control), 2, 5 and 10 g/kg diet for 60 days before being challenged with <em>V. harveyi</em>. Dietary PCE significantly improved (<em>P</em> &lt; 0.05) survival, with the dose of 10 g/kg showing the highest survival rate (90 %) when compared to the control (60 %). Additionally, hematological (red and white blood cell counts, hemoglobulin, packed cell volume, and mean corpuscular volume) and immunological (activities of lysozyme, phagocytic activity and respiratory burst, and serum total immunoglobulin) properties were significantly increased (<em>P</em> &lt; 0.05) in comparison to the control group. In contrast, serum aspartate aminotransferase and alanine aminotransferase levels, as well as glucose level were significantly reduced (<em>P</em> &lt; 0.05) in PCE-fed fish compared to the control group. Conclusively, the current study discovered that supplementing fish feed with <em>P. chinense</em> extract improves fish haemato-biochemical profile, immunocompetence and disease resistance to <em>V. harveyi</em> infection.</p></div>","PeriodicalId":73029,"journal":{"name":"Fish and shellfish immunology reports","volume":"5 ","pages":"Article 100118"},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49776056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stocking density affects immune and stress-related gene expression of Butter catfish (Ompok bimaculatus) fry in biofloc landscapes","authors":"Snigdha S. Majhi ,&nbsp;Soibam Khogen Singh ,&nbsp;Pradyut Biswas,&nbsp;Reshmi Debbarma,&nbsp;Janmejay Parhi,&nbsp;Ananya Khatei,&nbsp;Yumnam Abungcha Mangang,&nbsp;Gusheinzed Waikhom,&nbsp;Arun Bhai Patel","doi":"10.1016/j.fsirep.2023.100112","DOIUrl":"https://doi.org/10.1016/j.fsirep.2023.100112","url":null,"abstract":"<div><p>Scientific research into fish wellness is critical, and the concerns about crowding-related stress due to increased stocking density are inevitable. Taking this into consideration, the study defines the physiological signature of <em>Ompok bimaculatus</em> (Butter catfish) in a biofloc system when subjected to varying levels of stocking density. Fish (mean weight = 1.21 <em>g</em> ± 0.08, <em>n</em> = 600) were randomly stocked in 40-L glass aquaria at stocking densities of 0.5 g/L (T1), 1 g/L (T2), 1.5 g/L (T3), and 2 g/L (T4) and fed a 35% protein diet. After the 90-day trial, the physio-biochemical, molecular, and tissue-level changes were assessed. An integrated biomarker response (IBR) analysis for the key stress indicators aided us in better understanding them. There was a significant difference in blood count between T1 and T4 (total erythrocyte count, hemoglobin, and packed cell volume). T1 had higher levels of globulin and total plasma protein, but T2 had higher levels of albumin. Only in T1 did the respiratory burst and lysozyme activity appear to be higher (p &lt; 0.05). Increased stocking densities had a significant impact on the liver function enzymes, GOT and GPT (p &lt; 0.05). In comparison to lower densities (T1 &amp; T2), higher stocking density (T3 &amp; T4) was found to raise glucose and cortisol levels (p &lt; 0.05). Antioxidant enzymes such as catalase, glutathione-S-transferase, and malondialdehyde were found to be more pronounced in lower density tissues (T1). Furthermore, the IBR plots show that lower densities have better health than higher densities. At higher stocking densities, mRNA expression of <em>HSP70</em>, IL-1, and IL-20 increased (p &lt; 0.05) in kidney and liver tissues. The Nrf-2 and Tlr-9 genes were also upregulated. Also, when stocking density was increased, tissue-level histo-architectural changes were more pronounced than when stocking density was kept low. The findings of this study show that the welfare of Butter catfish cultured at high density in biofloc systems suffers from severe stress, and therefore draw more attention to the development of a species-specific standard rearing methodology in the pursuit of a profitable aqua-farming enterprise.</p></div>","PeriodicalId":73029,"journal":{"name":"Fish and shellfish immunology reports","volume":"5 ","pages":"Article 100112"},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49776060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Administration of probiotics affects Artemia franciscana metanauplii intestinal ultrastructure and offers resistance against a Photobacterium damselae ssp. piscicida induced oxidative stress response","authors":"Maria Touraki ,&nbsp;Anna Chanou ,&nbsp;Vasiliki Mavridou ,&nbsp;Vasiliki Tsertseli ,&nbsp;Maria Tsiridi ,&nbsp;Emmanuel Panteris","doi":"10.1016/j.fsirep.2023.100113","DOIUrl":"https://doi.org/10.1016/j.fsirep.2023.100113","url":null,"abstract":"<div><p>The effects of <em>Photobacterium damselae</em> ssp<em>. piscicida</em> (Phdp) on immune responses and intestinal ultrastructure of <em>Artemia franciscana</em> following infection and their amelioration by the probiotic bacteria <em>Bacillus subtilis, Lactobacillus plantarum</em> and <em>Lactococcus lactis</em> were evaluated. Pathogen growth inhibition in coculture with each probiotic and its virulence against <em>Artemia</em> were confirmed with an LC₅₀ of 10⁵ CFU mL⁻¹. Phdp administration to <em>Artemia</em> at sublethal levels resulted in depletion of superoxide dismutase, glutathione reductase, glutathione transferase and phenoloxidase activities, extensive lipid peroxidation and reduced survival. Following a combined administration of each probiotic and the pathogen, enzyme activities and survival were significantly higher, while lipid peroxidation was reduced, compared to the infected group with no probiotic treatment (<em>P</em> &lt; 0.05). The transmission electron microscopy study revealed that pathogen infection resulted in disarranged and fragmented microvilli, formation of empty or pathogen containing cytoplasmic vacuoles and damaged mitochondria. In the probiotic-treated and Phdp-infected series, intestinal cells showed normal appearance, except for the presence of pathogen-containing vacuoles and highly ordered but laterally stacked microvilli. The results of the present study indicate that Phdp induces cell death through an oxidative stress response and probiotics enhance <em>Artemia</em> immune responses to protect it against the Phdp induced damage.</p></div>","PeriodicalId":73029,"journal":{"name":"Fish and shellfish immunology reports","volume":"5 ","pages":"Article 100113"},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49866171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary protein, lipid and insect meal on growth, plasma biochemistry and hepatic immune expression of lake whitefish (Coregonus clupeaformis)","authors":"Yubing Chen ,&nbsp;Rebecca Lawson ,&nbsp;Umesh Shandilya ,&nbsp;Marcia A. Chiasson ,&nbsp;Niel A. Karrow ,&nbsp;David Huyben","doi":"10.1016/j.fsirep.2023.100111","DOIUrl":"https://doi.org/10.1016/j.fsirep.2023.100111","url":null,"abstract":"<div><p>Studies are lacking that investigate the dietary nutrient requirements of lake whitefish (<em>Coregonus clupeaformis</em>), a newly farmed fish species in Ontario, Canada. Dietary levels of protein and lipid must be optimized to ensure high growth performance for the commercial success of this species. Additionally, the inclusion of insect meal in the diet may improve growth and immune response. The objective of this study was to evaluate the effects of dietary protein:lipid ratios and insect meal as a feed additive on the growth performance and hepatic immune function of juvenile lake whitefish (301 ± 10 g). A 16-week (112 day) trial was performed with five diets including a commercial control diet (BCC), and four experimental diets with high or low levels of protein (54 and 48%, respectively) and lipid (18 and 12%, respectively). The high protein dietary groups contained 5% of full-fat black soldier fly larvae (<em>Hermetia illucens</em>). Fish weights, viscera, liver, and blood were collected for further analysis. Specific growth rate, thermal growth coefficient and weight gain were significantly higher in fish fed with the BCC and high protein high lipid (HPHL) diets. However, viscerosomatic index was found to be significantly higher in fish fed the BCC diet, thus HPHL is more optimal for non-visceral weight gain. Higher levels of plasma phosphorus, aspartate aminotransferase and potassium indicated poor growth and stress in fish fed low lipid diets. Relative expression of <em>HSP70</em>, involved in cellular repair, was significantly downregulated in fish fed high lipid diets, and no effects were found on the expression of innate immune and oxidative stress genes. Also, <em>IL8 (CXCL8)</em> and <em>catalase</em> were upregulated (non-significant) in fish fed the HPHL diet with the largest weight gain. No effects of insects were found on growth, plasma biochemistry or gene expression, which suggests 5% dietary inclusion was too low. Overall, we recommend a HPHL diet for the cultivation of lake whitefish based on improved growth performance, low viscera weight, improved plasma biochemistry and downregulation of cellular repair genes.</p></div>","PeriodicalId":73029,"journal":{"name":"Fish and shellfish immunology reports","volume":"5 ","pages":"Article 100111"},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49866172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of pH and salinity on survival, growth, and enzyme activities in juveniles of the sunray surf clam (Mactra chinensis Philippi)","authors":"Yuanyuan Dai ,&nbsp;Yubo Dong ,&nbsp;Feng Yang ,&nbsp;Zhongzhi Chen ,&nbsp;Jia Jia ,&nbsp;Huimin Wu ,&nbsp;Zilong Chen","doi":"10.1016/j.fsirep.2023.100114","DOIUrl":"https://doi.org/10.1016/j.fsirep.2023.100114","url":null,"abstract":"<div><p>The study investigated the impact of salinity and pH changes on the survival, growth, and antioxidant enzyme activity in <em>Mactra chinensis</em> Philippi (1.00 ± 0.10 cm shell length, 0.75 ± 0.04 cm shell height), a marine clam species. Juveniles were exposed to various pH levels (5.4 - 9.6) and salinities (5 - 35 psu) for up to 20 days at 19 ± 0.5 ˚C. The individual effect of salinity and pH on juveniles were evaluated under pH 8.0 and salinity 30 psu, respectively. The results indicated that the highest survival rates were observed at pH 8.0 (85%, salinity = 30 psu) and salinity 30 psu (95%, pH = 8.0). The survival rates were significantly reduced at extreme pH (≤ 7.2; ≥ 8.4) and salinities (≤ 15; 35 psu). Additionally, oxidative stress was observed in clams exposed to low pH and salinity as indicated by the decreased activities of the antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD). Notably, no significant difference in relative growth rates was observed between salinity 25 and 30 psu, between pH 7.8/8.4 and pH 8.0. Our results provide information on potential impact of pH and salinity changes on economically important bivalve species and may be used to optimize pH and salinity in aquaculture.</p></div>","PeriodicalId":73029,"journal":{"name":"Fish and shellfish immunology reports","volume":"5 ","pages":"Article 100114"},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49776058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of non-ribosomal and polyketide biosynthetic genes in bacteria from green mud crab Scylla serrata gut microbiome and their antagonistic activities","authors":"Shabreen Banu ,&nbsp;Shivakiran Alva ,&nbsp;Prathiksha J. Prabhu ,&nbsp;Sreedharan Krishnan ,&nbsp;Madhu K. Mani","doi":"10.1016/j.fsirep.2023.100104","DOIUrl":"https://doi.org/10.1016/j.fsirep.2023.100104","url":null,"abstract":"","PeriodicalId":73029,"journal":{"name":"Fish and shellfish immunology reports","volume":"5 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49776062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial activity and anxiolytic-like effect of Ziziphus joazeiro Mart. leaves in adult zebrafish (Danio rerio)","authors":"Antônio Barros de Souza ,&nbsp;Jacqueline Cosmo Andrade Pinheiro ,&nbsp;Juliete Bezerra Soares ,&nbsp;José Ismael Feitosa de Araújo ,&nbsp;Sandra Maria Barbosa de Araújo ,&nbsp;Francisco Lucas Alves Batista ,&nbsp;Kalina Kelma Oliveira de Sousa ,&nbsp;Saulo Relison Tintino ,&nbsp;Isaac Moura Araujo ,&nbsp;Francisco Ernani Alves Magalhães ,&nbsp;Laura Hévila Inocencio Leite ,&nbsp;Francisco Roberto de Azevedo","doi":"10.1016/j.fsirep.2023.100108","DOIUrl":"https://doi.org/10.1016/j.fsirep.2023.100108","url":null,"abstract":"<div><p><em>Ziziphus joazeiro</em> Mart. is an endemic plant of the Caatinga that presents a great socioeconomic importance for the Northeast and Semiarid Region of Brazil. In view of this, this study aimed to evaluate the antibacterial activity and anxiolytic-like effects of <em>Ziziphus joazeiro</em> Mart leaves in adult zebrafish (<em>Danio rerio</em>). The characterization of the main classes of metabolites was performed through chemical reactions. The antibacterial and antibiotic potentiating activity was evaluated by broth microdilution assays. The 96 h acute toxicity, open field test and anxiety models test was evaluated in vivo on adult zebrafish. The results obtained in the phytochemical prospection evidenced the presence of flobabenic tannins, leucoanthocyanidins, flavonois, flavonones, catechins, alkaloids, steroids, and triterpenoids. EEFZJ did not show antibacterial activity for all microorganism tested (MIC ≥ 1024 µg/mL), but reduced the concentration required for bacterial growth inhibition in combination with gentamicin and norfloxacin against multidrug-resistant strains of <em>S. aureus</em> (SA10) and <em>E. coli</em> (EC06), exhibiting synergistic effect with these antibiotics (<em>p</em>&lt;0.0001). In the tests in vivo, EEFZJ was found to be nontoxic, performing reduced locomotor activity and demonstrated an anxiolytic-like effect in adult zebrafish via GABAergic and Serotoninergic systems (5-HT₁, 5-HT_2A/2C and 5-HT_3A/3B).</p></div>","PeriodicalId":73029,"journal":{"name":"Fish and shellfish immunology reports","volume":"5 ","pages":"Article 100108"},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49776063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular cloning, characterization and gene expression analysis of twelve interleukins in obscure puffer Takifugu obscurus","authors":"Ying Huang ,&nbsp;Peng Luo ,&nbsp;Fu-Hui Jiang ,&nbsp;Hui-Ze Gao ,&nbsp;Li-Fan Cui ,&nbsp;Zhe Zhao","doi":"10.1016/j.fsirep.2023.100103","DOIUrl":"https://doi.org/10.1016/j.fsirep.2023.100103","url":null,"abstract":"<div><p>Interleukins (ILs) are a subgroup of secreted cytokines, which are molecules involved in the intercellular regulation of the immune system. In this study, 12 IL homologs were cloned and functionally identified from obscure puffer <em>Takifugu obscurus</em>, and they were termed as <em>ToIL-1β, ToIL-1, ToIL-6, ToIL-10, ToIL-11, ToIL-12, ToIL-17, ToIL-18, ToIL-20, ToIL-24, ToIL-27</em>, and <em>ToIL-34</em>. Multiple alignment results showed that except for ToIL-24 and ToIL-27, other deduced ToIL proteins shared typical characteristics and structure with other known fish ILs. Phylogenetic analysis revealed that 12 ToILs were evolutionarily closely related to their counterparts in other selected vertebrates. Tissue distribution assay demonstrated that the mRNA transcripts of most <em>ToIL</em> genes were constitutively expressed in all tissues examined, with relatively high expression in immune tissues. Following <em>Vibrio harveyi</em> and <em>Staphylococcus aureus</em> infection, the expression levels of 12 <em>ToILs</em> in the spleen and liver were significantly upregulated, and their response over time varied. Taken together, these data were discussed accordingly with the <em>ToIL</em> expression and the immune response under the different situations tested. The results suggest that the 12 <em>ToIL</em> genes are involved in the antibacterial immune response in <em>T. obscurus</em>.</p></div>","PeriodicalId":73029,"journal":{"name":"Fish and shellfish immunology reports","volume":"5 ","pages":"Article 100103"},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49776048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic map of the differentially expressed proteins in the skin of Ctenopharyngodon idella against Aeromonas hydrophila infection","authors":"Shandana Ali ,&nbsp;Farman Ullah Dawar ,&nbsp;Waheed Ullah ,&nbsp;Maizom Hassan ,&nbsp;Kalim Ullah ,&nbsp;Zhe Zhao","doi":"10.1016/j.fsirep.2023.100122","DOIUrl":"https://doi.org/10.1016/j.fsirep.2023.100122","url":null,"abstract":"<div><p>The skin mucus of fish is an important part of the innate immune system, which is poorly understood at the proteomic level. The study established a complete map of the proteins in the skin mucus of <em>Ctenopharangdon idella</em> (<em>C. idella</em>) and discussed the Differentially Expressed Proteins (DEPs) after <em>Aeromonas hydrophila</em> (<em>A. hydrophila</em>) infection. Using Label Free Liquid Chromatography-Mass Spectrometry (LC–MS/MS) analysis, a total of 126 proteins were identified as differentially expressed, 89 proteins of which were upregulated, and 37 proteins were downregulated. Functional annotations of DEPs showed that the upregulated proteins in the skin mucus of the treated group were mostly associated with complement system and cytoskeleton proteins, whereas downregulated proteins were associated with metabolism. The key upregulated immune proteins were transferrin variant C, lysozyme g, annexin A11, 26S proteasome non-ATPase regulatory subunit 8, hypothetical protein ROHU_000884, 60S ribosomal L7a, calpain-2 catalytic subunit-like protein, calpain-9-like protein, complement component C9, complement C3, cathepsin S, cathepsin Z, 14 kDa apolipo, heat shock protein and intelectin, whereas, leukocyte elastase inhibitor, annexin A11, C-factor-like protein, biotinidase isoform X1 and epidermal growth factor receptor substrate 15-like were the downregulated proteins. Moreover, we for the first-time report proteins such as coactosin, lamin-B2 and kelch 12, which were never reported in fish. Our study directly pointing out the possible immunological biomarkers in the skin mucus of <em>C. idella</em> after <em>A. hydrophila</em> treatment. Each of the protein we report in this study could be used as base to establish their mechanism of action during bacterial infection that may contribute to the strategies against bacterial prevention and control in fishes.</p></div>","PeriodicalId":73029,"journal":{"name":"Fish and shellfish immunology reports","volume":"5 ","pages":"Article 100122"},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667011923000427/pdfft?md5=4f21b5e3518429dafb1f679f1efd615c&pid=1-s2.0-S2667011923000427-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92019986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel liver-specific immunoglobulin heavy chain-like gene in a cartilaginous fish","authors":"Hidehiro Kondo,&nbsp;Haruka Kamei,&nbsp;Fuyuka Murotani,&nbsp;Reiko Nozaki,&nbsp;Ryosuke Yazawa,&nbsp;Reoto Tani,&nbsp;Keiichiro Koiwai,&nbsp;Ikuo Hirono","doi":"10.1016/j.fsirep.2023.100102","DOIUrl":"10.1016/j.fsirep.2023.100102","url":null,"abstract":"<div><p>We identified a novel immunoglobulin (Ig) heavy chain-like gene (tsIgH) expressed in the liver of the banded houndshark <em>Triakis scyllium</em> by preliminary transcriptomic analysis. The tsIgH gene showed less than 30% of amino acid identities to Ig genes of the shark. The gene encodes one variable domain (VH) and three conserved domains (CH1-CH3) with a predicted signal peptide. Interestingly, this protein has only one cysteine residue in a linker region between VH and CH1 other than those required for the formation of the immunoglobulin domain. Genome sequencing revealed that each of the domains was encoded by a corresponding single exon, and the exon-intron structures of the homologues are conserved in the other cartilaginous fishes. By RT-qPCR analysis, the transcript of the tsIgH gene was observed only in the liver, while that of the IgM was mainly detected in the epigonal organ, liver, and spleen. The novel Ig-heavy chain-like gene in cartilaginous fish may provide new clues to the evolution of immunoglobulin genes.</p></div>","PeriodicalId":73029,"journal":{"name":"Fish and shellfish immunology reports","volume":"5 ","pages":"Article 100102"},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/84/cf/main.PMC10331284.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9814452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}