Extracellular vesicle最新文献

{"title":"Accelerating extracellular vesicle research and biotechnological applications using synthetic biology approaches","authors":"Richard J.R. Kelwick ,&nbsp;Alexander J. Webb ,&nbsp;Paul S. Freemont","doi":"10.1016/j.vesic.2024.100050","DOIUrl":"10.1016/j.vesic.2024.100050","url":null,"abstract":"<div><p>Can a combination of the extracellular vesicle (EV) research community’s latest consensus guidance (MISEV2023) along with synthetic biology’s engineering approaches and responsible innovation framework help to accelerate future EV-based industrial and medical applications?</p></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"4 ","pages":"Article 100050"},"PeriodicalIF":0.0,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773041724000179/pdfft?md5=720e1cb2e048bcb19c26bb9c90ec5840&pid=1-s2.0-S2773041724000179-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142238163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Extracellular vesicle microRNAs and RNA binding proteins on glioblastoma dynamics and therapeutics development","authors":"Shilpi Singh ,&nbsp;Clark C. Chen ,&nbsp;Stefan Kim ,&nbsp;Amar Singh ,&nbsp;Gatikrushna Singh","doi":"10.1016/j.vesic.2024.100049","DOIUrl":"10.1016/j.vesic.2024.100049","url":null,"abstract":"<div><p>The intricate interplay between extracellular vesicles (EVs), microRNAs, and RNA binding proteins (RBPs) constitutes a crucial mechanism in glioblastoma pathogenesis. The regulated sorting of microRNAs into EVs, influenced by RBPs and specific RNA motifs, plays a pivotal role in intercellular communication and significantly impacts various biological processes associated with glioblastoma development. Glioblastoma cells exploit this mechanism to exchange genetic information, influencing tumor progression, heterogeneity, and treatment response. This review highlights the dysregulation of EV-encapsulated microRNAs as a modulator of key signaling the disease. The versatile role of EV-microRNAs extends to impacting glioma behavior, immune response, angiogenesis, and serving as valuable diagnostic, prognostic, and therapeutic targets. Furthermore, the involvement of RBPs in microRNA sorting into EVs presents therapeutic targets that bridge cellular signaling with EV cargo composition. This comprehensive understanding and the strategic approaches targeting the complex interactions within the EV-microRNA-RBP axis opens promising avenues for advancing diagnostics and developing targeted therapies against the heterogeneous nature of glioblastoma and enhance treatment efficacy. The personalized and effective treatment strategies that may emerge from this research have the potential to revolutionize the approach to combating this devastating brain cancer.</p></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"4 ","pages":"Article 100049"},"PeriodicalIF":0.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773041724000167/pdfft?md5=65f1184d771358a37b490b40b3ddee79&pid=1-s2.0-S2773041724000167-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142161956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An extracellular vesicle based hypothesis for the genesis of the polycystic kidney diseases","authors":"Marie C. Hogan ,&nbsp;Christopher J. Ward","doi":"10.1016/j.vesic.2024.100048","DOIUrl":"10.1016/j.vesic.2024.100048","url":null,"abstract":"&lt;div&gt;&lt;p&gt;Autosomal dominant polycystic kidney (ADPKD) disease is the commonest genetic cause of kidney failure (affecting 1:800 individuals) and is due to heterozygous germline mutations in either of two genes, &lt;em&gt;PKD1&lt;/em&gt; and &lt;em&gt;PKD2&lt;/em&gt;. Homozygous germline mutations in &lt;em&gt;PKHD1&lt;/em&gt; are responsible for autosomal recessive polycystic kidney (ARPKD) disease a rare (1:20,000) but severe neonatal disease. The products of these three genes, &lt;em&gt;PKD1&lt;/em&gt; (polycystin-1 (PC1 4302(3)aa)), &lt;em&gt;PKD2&lt;/em&gt; (polycystin-2 (PC2 968aa)) and &lt;em&gt;PKHD1&lt;/em&gt; (fibrocystin (4074aa)) are all present on extracellular vesicles (EVs) termed, PKD-exosome-like vesicles (PKD-ELVs). PKD-ELVs are defined as 100&lt;!--&gt; &lt;!--&gt;nm PC1/PC2/CD133 and fibrocystin positive EVs which are shed into the urine from the apical plasma membrane of proximal tubule (PT) cells. PKD-ELVs are therefore ectosomes and are distinct from classical exosomes from the multivesicular body. PC1, PC2, fibrocystin and exosomal polycystin-1 interacting protein (EPIC, CU062) form a higher order ion channel complex termed the polycystin complex (PCC) on the surface of the PKD-ELV. We hypothesize that the PCC is involved in the generation of the PKD-ELV and is a structural component thereof. The PCC has also been detected on the primary cilium, a hair like 9+0 microtubule based structure present on all cells except hepatocytes. In kidney epithelial cells, the primary cilium protrudes into the lumen of the tubule where it regulates planar cell polarity (PCP) and tubule lumen diameter. Here we present a theory that explains the presence of the PCC on PKD-ELVs and primary cilia as well as other cryptic aspects of ADPKD and ARPKD. We suggest that the fundamental role of the PCC is to assemble PKD-ELVs on the plasma membrane and then shed them into the extracellular space or the lumen of the tubule. The resultant PKD-ELVs can have multiple functions in different biological contexts. One of the roles of the resultant PKD-ELVs is to generate a planar cell polarity (PCP) signaling gradient along kidney tubules in developing or regenerating kidney. This is mediated via an adhesion event between the PKD-ELV and primary cilium. Defects in the primary cilium or PKD-ELV assembly lead to cystogenesis, the major feature of ADPKD. The other important role for the PCC dependent PKD-ELV is the detection, packaging and extrusion of defective mitochondria. The PKD-ELV is also critical in the transfer of mRNA and miRNAs between cells and as a vector for extracellular proteinases and hyaluronidases involved in tissue remodeling. A PKD-ELV centric view of polycystic disease (EV theory) can explain the requirement for primary cilium function in ADPKD (where the primary cilium is the PKD-ELV receptor), the observation of defective mitochondria in the disease, the abnormalities detected in the extracellular matrix (ECM) as well as the resistance to carcinoma noted in ADPKD patients and individuals carrying &lt;em&gt;PKHD1&lt;/em&gt; mutations,","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"4 ","pages":"Article 100048"},"PeriodicalIF":0.0,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773041724000155/pdfft?md5=48b923544849d6c3c1821ca5848edc6c&pid=1-s2.0-S2773041724000155-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141954453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificially engineered vesicular vehicles for targeted therapy","authors":"Zhigang Wang ,&nbsp;Yufei Yan ,&nbsp;Rui Li,&nbsp;Ying Zhu,&nbsp;Gen Yang","doi":"10.1016/j.vesic.2024.100047","DOIUrl":"10.1016/j.vesic.2024.100047","url":null,"abstract":"<div><p>Vesicular vehicles have been one of the most potential platforms for therapeutic delivery. Here, we commentate on recent advances in the development of therapeutic vesicular delivery platforms, emphasizing two intriguing works, namely, the chimeric nanobody-decorated immunoliposomes (Wan et al.) and the retrovirus-like capsids incorporated extracellular vesicles (Jiang et al.). In the work of Wan et al., the researchers proposed a novel strategy for antitumor immunoliposome construction by the one-step self-assembly of lipids with rationally designed chimeric nanobody that comprises a nanobody against human epidermal growth factor receptor 2, a flexible peptide linker, and a human single transmembrane domain. By leveraging the natural cell systems (e.g., <em>E coli.</em>) to synthesize the chimeric nanobody, this technique provides a facile and robust strategy for producing immunoliposome platforms, ensuring suitability for industrial manufacturing and distribution, regulatory compliance, and clinical use. In the work of Jiang et al., taking advantage of the natural extracellular vesicle biogenesis system, the researchers introduced the activity-regulated cytoskeleton-associated protein inside the vesicle lumen and thus constructed endogenous retrovirus-like vesicles for mRNA drug delivery to neurons. This technique offers a significative strategy for developing sophisticated delivery platforms, manifesting broad application potential for different disease therapeutics. Overall, these two works have represented two typical paradigms for the artificial construction of vesicular vesicles. Considering their complementary merits, in the future, the integration of artificial engineering technologies and natural biosynthesis systems is anticipated to be a promising strategy to expedite the development of potent delivery systems.</p></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"4 ","pages":"Article 100047"},"PeriodicalIF":0.0,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773041724000143/pdfft?md5=892695f80d592b8b035d791f34e79689&pid=1-s2.0-S2773041724000143-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141960705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stem cell-derived exosomes prevent the development of thoracic aortic aneurysm/dissection by inhibiting AIM2 inflammasome and pyroptosis","authors":"Lin Lu ,&nbsp;Feng Liu ,&nbsp;Weiliang Wu ,&nbsp;Yu Zhang ,&nbsp;Bin Liu ,&nbsp;Qingfang Han ,&nbsp;Tonggan Lu ,&nbsp;Huiling Zhang ,&nbsp;Xi-yong Yu ,&nbsp;Yangxin Li","doi":"10.1016/j.vesic.2024.100046","DOIUrl":"https://doi.org/10.1016/j.vesic.2024.100046","url":null,"abstract":"<div><p>Thoracic aortic aneurysm/dissection (TAAD) is a severe vascular condition associated with life-threatening complications, and its underlying molecular mechanisms remain largely unexplored. Previous research indicates that the aberrant activation of cytosolic DNA and its receptors plays a crucial role in vascular inflammation and dysfunction. Specifically, Absent in Melanoma 2 (AIM2), an intracellular DNA receptor, can trigger the inflammasome pathway, leading to extracellular matrix destruction. In this investigation, we delved into the mechanism underlying AIM2 activation in TAAD development and explored the potential of exosomes to impede TAAD progression by suppressing AIM2 expression. Our findings revealed that heightened AIM2 expression and activation contribute to TAAD development by fostering vascular inflammation and disrupting vascular homeostasis. Activated AIM2 induces pyroptosis through the recruitment of the deubiquitination enzyme USP21, which stabilizes AIM2 by reducing its ubiquitination and degradation. Moreover, we demonstrated that exosome-derived miR-485-5p exerts an anti-inflammatory and protective effect on the thoracic aorta by inhibiting AIM2 activation. This study introduces novel perspectives for the treatment of TAAD.</p></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"4 ","pages":"Article 100046"},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773041724000131/pdfft?md5=8a900f4bbb54766ace6f15d1f225c232&pid=1-s2.0-S2773041724000131-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141484849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of human stem cell-derived extracellular vesicles in idiopathic pulmonary fibrosis","authors":"Kyoung Soo Lee ,&nbsp;Seung Ho Yeom ,&nbsp;Min Kang Kim ,&nbsp;Chang Hee Woo ,&nbsp;Young Chan Choi ,&nbsp;Ji Suk Choi ,&nbsp;Yong Woo Cho","doi":"10.1016/j.vesic.2024.100045","DOIUrl":"https://doi.org/10.1016/j.vesic.2024.100045","url":null,"abstract":"<div><p>Idiopathic pulmonary fibrosis (IPF) is a lethal and chronic lung disease that occurs due to persistent epithelial cell injury and abnormal extracellular matrix (ECM) deposition. Extracellular vesicles (EVs) have been proposed as a potential therapeutic option of IPF because of their functions, such as anti-inflammation, anti-fibrosis, microenvironment regulation, and tissue repair. In this study, we investigated the therapeutic potential of human adipose-derived mesenchymal stem cell (AD-MSC) EVs in IPF model. AD-MSC EVs were isolated by a multi-filtration system based on the tangential flow filtration (TFF) and characterized using transmission electron microscopy (TEM), dynamic light scattering (DLS), flow cytometry analysis, zeta potential, and small RNA sequencing. <em>In vitro</em> analysis reveals that AD-MSC EVs treatments inhibited migration of pulmonary fibroblasts and myofibroblast differentiation through down regulation of fibrosis-associated TGF-β and WNT signaling. In addition, inhalation treatment of AD-MSC EVs significantly alleviated bleomycin (BLM)-induced pulmonary fibrosis. These results reveal that AD-MSC EVs are highly promising as a treatment option for pulmonary fibrosis.</p></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"4 ","pages":"Article 100045"},"PeriodicalIF":0.0,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S277304172400012X/pdfft?md5=d3ec1dc4b1a879c67ab2d843a901c474&pid=1-s2.0-S277304172400012X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141484868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Host innate immune systems gather intel on invading microbes via pathogen-derived extracellular vesicles","authors":"Geneva N. Kwaku ,&nbsp;Rebecca A. Ward ,&nbsp;Jatin M. Vyas ,&nbsp;Hannah Brown Harding","doi":"10.1016/j.vesic.2024.100043","DOIUrl":"https://doi.org/10.1016/j.vesic.2024.100043","url":null,"abstract":"<div><p>Extracellular vesicles (EVs) are membrane-bound vesicles released into the extracellular milieu from various cell types including host cells and pathogens that infect them. As carriers of nucleic acids, proteins, lipids, metabolites, and virulence factors, EVs act as delivery vehicles for intercellular communication and quorum sensing. Innate immune cells have the capacity to intercept, internalize, and interpret ‘messages’ contained within these EVs. This review categorizes the ability of EVs secreted by bacterial, parasitic, and fungal pathogens to trigger both pro- and anti-inflammatory innate immune responses in the host. Understanding molecular pathways and inflammatory responses activated in innate immune cells upon pathogen-derived EV stimulation is critical to gain insight into potential therapeutics and combat these infectious diseases.</p></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"3 ","pages":"Article 100043"},"PeriodicalIF":0.0,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773041724000106/pdfft?md5=78b753990842574e97fefc41590a2b3c&pid=1-s2.0-S2773041724000106-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141066937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From hope to healing: Exploring the therapeutic potential of exosomes in spinal cord injury","authors":"Nidhi Singh ,&nbsp;Lahanya Guha ,&nbsp;Hemant Kumar","doi":"10.1016/j.vesic.2024.100044","DOIUrl":"https://doi.org/10.1016/j.vesic.2024.100044","url":null,"abstract":"<div><p>Spinal cord injury (SCI) is the damage to the spinal cord causing paralysis below the injury site causing compromised patient life quality. Exosomes are nanosized vesicles released during physiological or pathological processes. They present a promising avenue for therapeutic drug delivery due to their tissue specificity and biocompatibility, offering enhanced efficacy with reduced accumulation-based toxicity. In SCI, exosomes target inflammation, apoptosis, microglial polarization, and autophagy, contributing to therapeutic interventions. This article comprehensively explores the pathophysiology of SCI, diverse exosome synthesis processes, their limitations and challenges to overcome, and the potential of exosomes from various cellular sources in SCI treatment.</p></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"3 ","pages":"Article 100044"},"PeriodicalIF":0.0,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773041724000118/pdfft?md5=2ae635bc0b513989376cf2c164ba971b&pid=1-s2.0-S2773041724000118-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140913870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the intricacies of bone homeostasis: Epigenetic regulation, extracellular vesicles, and angiogenesis integration","authors":"Célio Junior da Costa Fernandes","doi":"10.1016/j.vesic.2024.100042","DOIUrl":"https://doi.org/10.1016/j.vesic.2024.100042","url":null,"abstract":"<div><p>Bone homeostasis is a complex process involving the coordinated actions of various bone cells and their interactions with the surrounding microenvironment. Epigenetic regulation, specifically through DNA methylation, histone acetylation, microRNAs, and long non-coding RNAs, has been identified as a crucial factor in maintaining bone health. Furthermore, the interplay between bone cells and angiogenesis, the process of forming new blood vessels, plays a critical role in maintaining bone health. Epigenetic factors have been found to regulate angiogenesis in bone tissue. Extracellular vesicles derived from different cell types, such as osteoblasts, endothelial cells, and mesenchymal stem cells, are also important in bone homeostasis. These vesicles transport bioactive molecules, including microRNAs, growth factors, and cytokines, which regulate angiogenesis and influence the function of bone cells. Understanding the epigenetic control of gene expression and the role of extracellular vesicles in bone homeostasis has significant implications for the development of therapeutic strategies for bone disorders. This knowledge is particularly valuable in the context of aging, metabolic disorders, and pathological disruptions of bone homeostasis.</p></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"3 ","pages":"Article 100042"},"PeriodicalIF":0.0,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S277304172400009X/pdfft?md5=9e81a1d892cc1d259549aeb9203ca394&pid=1-s2.0-S277304172400009X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140901820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular vesicle lyophilization for enhanced distribution to the point of care","authors":"Morgane E. Golan ,&nbsp;Steven L. Stice","doi":"10.1016/j.vesic.2024.100041","DOIUrl":"https://doi.org/10.1016/j.vesic.2024.100041","url":null,"abstract":"<div><p>Therapeutic extracellular vesicles (EVs) are in clinical trials and if successful, EVs wider use will be largely affected by costs to patients/insurers and accessibility. EV preservation-storage efficiencies directly factor into both of these considerations. EV cryopreservation drives up costs and relies on the pharmaceutical cold chain, which is expensive and among the most pressing barriers to widespread access of medicines. Lyophilization preserves biological materials at room temperature, thereby enhancing storage while circumventing the cold chain. Here we describe the factors influencing EV storage and lyophilization processes which may improve accessibility of EV therapies.</p></div>","PeriodicalId":73007,"journal":{"name":"Extracellular vesicle","volume":"3 ","pages":"Article 100041"},"PeriodicalIF":0.0,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773041724000088/pdfft?md5=a734182959042032c44efc4b546c400b&pid=1-s2.0-S2773041724000088-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140880327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}