Environmental science. Advances最新文献

{"title":"Insights into complementary exposomic targeted analysis and suspect screening approaches: a case study examining human serum for chemicals with LC-IMS-MS.","authors":"James N Dodds, Nikki Barlow, Kara M Joseph, Sarah J Rehm, Weihsueh A Chiu, Gang Han, Yu-Syuan Luo, Kangmin Zhu, Warren Casey, Ivan Rusyn, Erin S Baker","doi":"10.1039/d6va00088f","DOIUrl":"10.1039/d6va00088f","url":null,"abstract":"<p><p>Although PFAS exposure is widespread in the general population, concern is heightened for individuals with unique occupational exposure scenarios. Accordingly, the PROject for Military Exposures and Toxin History Evaluation in US service members (PROMETHEUS) study is evaluating whether serum chemical exposure profiles correlate with cancer incidence in large cohorts (typically hundreds of samples per analysis) among military service members who may experience distinct occupational and environmental exposures. Here we describe analytical workflow development and results from a pilot subset (<i>n</i> = 36) of human serum samples using an integrated targeted and suspect-screening LC-IMS-MS platform. Serum (50 µL) was extracted by acid-assisted protein precipitation with isotopically labeled internal standards, concentrated, and analyzed by LC coupled to an Agilent 6560 IM-QTOF. Targeted PFAS quantitation was performed using matrix-matched calibration curves and was benchmarked against NIST SRM 1957 to assess method accuracy. Across the samples, the targeted panel captured predominantly legacy PFAS as anticipated noting their prevalence in prior studies (<i>e.g.</i>, PFOS, PFOA, 8 : 2 FTS, <i>N</i>-MeFOSAA, <i>etc.</i>). Ultra-short-chain PFAS presented class-specific analytical challenges; trifluoromethanesulfonic acid (TFMS) was observed, whereas trifluoroacetic acid (TFA) eluted near the void volume and exhibited pronounced clustering in the ion mobility dimension, precluding reliable quantitation. In parallel, CCS-based mobility filtering supported suspect screening against an exposomic library (∼1100 entries) to expand detectable chemical space beyond targeted PFAS. Suspect screening yielded 49 non-PFAS candidates meeting accurate mass and CCS agreement criteria, and correlation analysis recapitulated expected co-exposure groupings among legacy PFCAs/PFSAs and structurally related suspect analytes. Collectively, these results establish a scalable, CCS-informed LC-IM-MS workflow for integrated targeted PFAS quantitation and exposomic suspect screening, enabling higher-powered association testing in the full set of PROMETHEUS samples and other large-scale human biomonitoring studies.</p>","PeriodicalId":72941,"journal":{"name":"Environmental science. Advances","volume":" ","pages":""},"PeriodicalIF":4.4,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13088947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147724876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel MOF-integrated MXene-magnetite electrochemical platform for effective detection of chloramphenicol","authors":"Roxana Paz, Herlys Viltres, Nishesh Gupta, Carolina Leyva, Seshasai Srinivasan and Amin Reza Rajabzadeh","doi":"10.1039/D5VA00474H","DOIUrl":"https://doi.org/10.1039/D5VA00474H","url":null,"abstract":"<p >Chloramphenicol (CAP) contamination in natural waters presents an increasing concern due to its persistence and potential risks to ecosystems and human health. To address this challenge, an electrochemical sensing platform based on a Fe<small>₃</small>O<small>₄</small>@MXene@Fe-BDC composite modified screen-printed carbon electrode was developed and characterized. The hybrid material combines the high electrical conductivity of MXene, the magnetic and catalytic properties of Fe<small>₃</small>O<small>₄</small>, and the adsorption capabilities of the Fe-BDC metal–organic framework, resulting in a synergistic enhancement of sensing performance. Using differential pulse voltammetry, the sensor achieved a broad linear detection range from 1 to 309 µM, a low detection limit of 0.26 µM, and a high sensitivity of 0.95 µA µM<small>⁻¹</small> cm<small>⁻²</small>. X-ray photoelectron spectroscopy and electrochemical analyses revealed that functional groups such as carboxyl, phenolic, and Fe–O play a central role in CAP recognition through hydrogen bonding, π–π interactions, and metal–oxygen coordination pathways. The sensor also exhibited notable stability, retaining 95.7% of its initial response after 20 days, and performed reliably in complex water samples. These results demonstrate the potential of the Fe<small>₃</small>O<small>₄</small>@MXene@Fe-BDC architecture as an efficient and practical tool for monitoring chloramphenicol in environmental settings.</p>","PeriodicalId":72941,"journal":{"name":"Environmental science. Advances","volume":" 4","pages":" 1162-1173"},"PeriodicalIF":4.4,"publicationDate":"2026-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2026/va/d5va00474h?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147621004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Health risk assessment of polycyclic aromatic hydrocarbons in indoor dust from Okerenkoko community, Warri, Nigeria","authors":"Sisanmi Samuel Aghomi, Okpoebi Kenneth Berezi and Chilaka Diepreye","doi":"10.1039/D4VA00430B","DOIUrl":"https://doi.org/10.1039/D4VA00430B","url":null,"abstract":"<p >This study investigated the occurrence, sources, and health risks of polycyclic aromatic hydrocarbons (PAHs) in indoor dust from the Okerenkoko Community, Warri, Nigeria. Dust samples were collected from three locations: NMU Campus/Lodge (NMU CL), Zion Residential Area (ZA), and Okerenkoko II Area (Okk). Total PAH concentrations (∑PAHs) ranged from 0.0546 to 0.1005 mg kg<small>⁻¹</small>, with higher levels at ZA and Okk, reflecting spatial variability influenced by local anthropogenic activities, including artisanal crude oil refining, biomass burning, combustion-related emissions from marine transport, and domestic fuel use. Compositional analysis revealed a predominance of 3- to 5-ring PAHs, with low- and high-molecular-weight (LMW and HMW) species contributing almost equally (50.8% and 49.2%, respectively), suggesting mixed pyrogenic–petrogenic sources. Diagnostic ratios (<em>e.g.</em>, Ant/(Ant + Phe), Flt/(Flt + Pyr), BaA/(BaA + Chr), IP/(IP + BghiP)) indicated that NMU CL and ZA were mainly influenced by pyrogenic sources, whereas Okk reflected both pyrogenic and petroleum-related inputs. Principal component analysis (PCA) confirmed distinct contributions, with HMW PAHs associated with combustion processes and LMW PAHs linked to volatilization or indoor emissions. Health risk assessment showed that adults exhibited the highest incremental lifetime cancer risks (ILCRs), followed by children and adolescents, with total ILCRs ranging from 1.05 × 10<small>⁻⁷</small> to 1.90 × 10<small>⁻⁷</small> across all sites. Dermal contact and ingestion were identified as the dominant pathways, while inhalation contributed negligibly (≤10<small>⁻¹²</small>). For example, adults at Okk recorded the highest ILCR (1.90 × 10<small>⁻⁷</small>), driven largely by dermal exposure, whereas children's ILCRs ranged between 1.26 × 10<small>⁻⁷</small> and 1.75 × 10<small>⁻⁷</small>. Non-cancer risk evaluation indicated hazard indices (HI) for all age groups remained far below 1 (children: 3.61 × 10<small>⁻⁵</small> to 4.88 × 10<small>⁻⁵</small>), suggesting negligible non-carcinogenic risk. These findings reveal the complex nature of PAH sources in indoor dust and highlight the importance of dermal exposure alongside ingestion, particularly for adults. The results underscore the heightened exposure risks for vulnerable groups and the urgency of targeted environmental controls and community health interventions in the Niger Delta.</p>","PeriodicalId":72941,"journal":{"name":"Environmental science. Advances","volume":" 4","pages":" 1141-1161"},"PeriodicalIF":4.4,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2026/va/d4va00430b?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147621001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spectroscopic investigation of the effects of simulated open waste burning on the functional and surface chemistry of commercial polystyrene","authors":"Maycee Hurd, Xuewen Wang, Angelica Benavidez, Allyson L. McGaughey, Michael Spilde, José M. Cerrato, Jorge Gonzalez-Estrella and Eliane El Hayek","doi":"10.1039/D5VA00291E","DOIUrl":"10.1039/D5VA00291E","url":null,"abstract":"<p >We studied the spectroscopic changes of polystyrene (PS) plastic used for commercial food packaging after thermal oxidation. Thermal oxidation of plastic in the environment occurs during open waste burning (OWB), urban wildfires, and even the serving of hot food. Environmental thermally oxidized plastics are understudied and display unique functional and surface chemistry compared to plastics exposed to other environmental weathering processes. Near-surface X-ray photoelectron spectroscopy (XPS) C 1s high resolution analyses show an increase in the intensity of peaks greater than 285 eV, corresponding to C/O bonds after burning of PS at 350 and 425 °C. Bulk analyses using Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) Spectroscopy show that thermal oxidation decreased the alkene (3040–3000 cm<small>⁻¹</small>) and alkane (3000–2850 cm<small>⁻¹</small>) C–H stretch signals, suggesting PS chain scission. Morphology defects and fractures were observed in scanning electron microscopy (SEM) images, indicating that the degradation of PS can lead to fragmentation after burning. The outcome of this study furthers the understanding of the thermal oxidative degradation mechanisms during incineration of commercial PS materials, which can inform future risk assessments of public and environmental health associated with OWB.</p>","PeriodicalId":72941,"journal":{"name":"Environmental science. Advances","volume":" 4","pages":" 1050-1059"},"PeriodicalIF":4.4,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12970243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147438112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liver-related biomarkers mediate the potential effect of volatile organic compounds on an anti-aging hormone: a cross-sectional study","authors":"Shuaifei Ji, Yiming Bi, Bochen An, Jingcheng Zhou, Lixin Yu and Ding Zeng","doi":"10.1039/D5VA00270B","DOIUrl":"https://doi.org/10.1039/D5VA00270B","url":null,"abstract":"<p >Volatile Organic Compounds (VOCs) are a class of air pollutants that have adverse metabolic and endocrine effects. α-Klotho, an anti-aging hormone, is susceptible to environmental pollution. This study aimed to explore the association between urinary VOCs and serum α-Klotho levels and the underlying molecular mechanisms. 1680 individuals from the U.S. were included. The association between VOCs and α-Klotho was explored using multivariate linear regression. Weighted quantile sum (WQS) regressions were used to identify the important distribution of VOCs on α-Klotho, and Bayesian kernel machine regressions (BKMR) were implemented to assess the joint and univariate effects of all VOCs on α-Klotho. Mediation analyses of liver-related biomarkers in the effects of mixed and single VOCs on α-Klotho were also performed. The multivariate linear regression model showed that multiple VOCs were negatively associated with α-Klotho. The WQS models ascertained that <em>N</em>-acetyl-<em>S</em>-(2-carboxyethyl)-<small>L</small>-cysteine (CEMA), <em>N</em>-acetyl-<em>S</em>-(2-cyanoethyl)-<small>L</small>-cysteine (CYMA), and 2-aminothiazoline-4-carboxylic acid (ATCA) play dominant roles. In the BKMR model, we found that the joint effects of mixed VOCs were negatively associated with α-Klotho in the general population (40–79 years) and middle-aged population (40–59 years). Finally, mediation analyses showed that liver-related biomarkers exhibited a substantial mediation effect in the single ATCA on α-Klotho, with a proportion as high as 31.6% in the middle-aged population. Our study demonstrated that liver-related biomarkers mediate the influence of VOCs, especially ATCA, on α-Klotho. Our findings suggest that reducing VOC exposure may be a potential strategy to maintain α-Klotho levels.</p>","PeriodicalId":72941,"journal":{"name":"Environmental science. Advances","volume":" 4","pages":" 1189-1202"},"PeriodicalIF":4.4,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2026/va/d5va00270b?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147620992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of explainable artificial intelligence and machine learning in predicting wastewater treatment plant variables: a comparative study of small- and large-scale treatment plants","authors":"Fuad Bin Nasir and Jin Li","doi":"10.1039/D5VA00408J","DOIUrl":"https://doi.org/10.1039/D5VA00408J","url":null,"abstract":"<p >Explainable artificial intelligence (XAI) can play a significant role in the application of machine learning (ML) in wastewater treatment plants (WWTPs). The present research focuses on evaluating the performance and generalizability of widely used ML models for predicting key effluent quality variables at multiple WWTPs. Effluent variables including ammonia nitrogen (NH<small>₃</small>–N), biochemical oxygen demand (BOD), chemical oxygen demand (COD), total phosphorus (TP), and total suspended solids (TSS) were predicted using eXtreme Gradient Boosting (XGBoost) and Random Forest (RF) models. Several feature selections (FS) and XAI tools were used to understand the influence of input variables on target variables and the impact of input variables on model performance. The study demonstrates that XAI can enhance the understanding of model behavior by identifying key input variables, thereby supporting more informed and transparent decision-making at WWTPs. The study finds that XAI methods are effective in capturing the influence of variables regardless of the choice of model for variable prediction. XAI tools, SHapley Additive exPlanations (SHAP) and Local Interpretable Model-agnostic Explanations (LIME), are successful in providing deeper understanding of the key factors influencing ML model performance. The findings of this research could facilitate WWTP operation with better decision-making in choosing ML models to optimize treatment performance and improve environmental sustainability.</p>","PeriodicalId":72941,"journal":{"name":"Environmental science. Advances","volume":" 4","pages":" 1174-1188"},"PeriodicalIF":4.4,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2026/va/d5va00408j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147620997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the impacts of feed and species composition on greenhouse gas emission from freshwater aquaculture systems in Bangladesh","authors":"K. R. Luba, H. Rashid, M. S. Islam, M. Akter, R. Khatun, M. Zaman and M. M. R. Jahangir","doi":"10.1039/D5VA00215J","DOIUrl":"https://doi.org/10.1039/D5VA00215J","url":null,"abstract":"<p >Bangladesh, a major global aquaculture producer, is potentially a notable source of greenhouse gas (GHG) emissions from aquaculture, but no measured data have yet been reported. This study investigates hydrochemistry, dissolved GHG concentrations in water and GHG (CO<small>₂</small>, CH<small>₄</small>, and N<small>₂</small>O) emissions from four prevalent freshwater aquaculture systems in Mymensingh, Bangladesh, focusing on Indian major carps (IMCs), Pangasius catfish (PG), Climbing perch (CP) and Tilapia (T), along with the feed loads. Semi-structured interviews with farm managers yielded key feed input parameters, including consumption rates, protein levels, and feed conversion ratios. Dissolved GHG concentrations and atmospheric emissions were measured during 2023 to 2024 (spanning from the dry winter to the pre-monsoon season, with biweekly sampling) using the headspace extraction for dissolved gases and a 40 × 40 × 50 cm<small>³</small> acrylic floating chamber for surface fluxes, with gas chromatography. The results revealed that PG systems exhibited significantly higher CH<small>₄</small>, N<small>₂</small>O and CO<small>₂</small> emissions by 89–96, 59–75 and 66–78% than IMC and T systems, likely due to intensive sinking-feed use causing low dissolved oxygen, high electrical conductivity and mineral nitrogen, thereby promoting anaerobic conditions and enhanced methanogenesis and denitrification. In contrast, the IMC and T systems, utilizing more efficient floating feeds, showed comparatively lower emissions. The dissolved gas concentration analysis further complemented the surface gas emissions data, offering a holistic understanding of emissions from aquaculture ponds. The results suggest that optimizing feed composition and management can substantially reduce the aquaculture sector's impacts on climate change. By providing a detailed understanding of the GHG emissions from different aquaculture systems, this research contributes valuable insights for policymakers, industry stakeholders, and environmental scientists.</p>","PeriodicalId":72941,"journal":{"name":"Environmental science. Advances","volume":" 4","pages":" 1130-1140"},"PeriodicalIF":4.4,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2026/va/d5va00215j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147621003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidation of TcO2 and UO2 by aqueous Mn(iii)-citrate and Mn(iii)-tartrate under anoxic conditions: implications for technetium and uranium fate and transport","authors":"Zachary Murphy, Zachary Ronchetti, Cassandra Munoz, David Rai and Vasileios Anagnostopoulos","doi":"10.1039/D5VA00264H","DOIUrl":"https://doi.org/10.1039/D5VA00264H","url":null,"abstract":"<p >There are several United States Department of Energy (DOE) sites that have been dealing with the problem of legacy nuclear waste in the environment for many generations. Two major risk-driving radionuclides in this legacy waste are uranium and technetium-99. Due to their radioactivity, the increase in concentration of these radionuclides in the environment represents a significant concern to environmental health and safety. Uranium and technetium both have increased solubility and mobility in the environment when oxidized from U(<small>IV</small>) to U(<small>VI</small>) and from Tc(<small>IV</small>) to Tc(<small>VII</small>), respectively. This project investigated the role of Mn(<small>III</small>)-tartrate and Mn(<small>III</small>)-citrate in influencing the redox behavior and stability of uranium, from uranium dioxide (UO<small>₂</small>), and technetium, from technetium oxide (TcO<small>₂</small>). For each radionuclide, anaerobic batch kinetic experiments of UO<small>₂</small> and TcO<small>₂</small> in the presence of these Mn(<small>III</small>)-ligands were conducted at pH 8 and 10, and the radionuclide concentration in the aqueous phase was monitored over time. Both the Mn(<small>III</small>)-tartrate and Mn(<small>III</small>)-citrate complexes effectively induced dissolution of uranium and technetium, however, differences were seen with increasing ligand concentration and pH. Higher concentrations of the Mn(<small>III</small>)-ligand complexes resulted in higher concentrations of aqueous uranium or technetium over time, supporting Mn(<small>III</small>) induced oxidation of these radionuclides. Additionally, the Mn(<small>III</small>)-tartrate complex resulted in more oxidation at pH 8 for both uranium and technetium while the Mn(<small>III</small>)-citrate ligand provided more oxidation at pH 10 for uranium and was comparable for technetium. The data presented in this study is useful for creating expected uranium and technetium transport models based on the abundance of naturally occurring manganese species.</p>","PeriodicalId":72941,"journal":{"name":"Environmental science. Advances","volume":" 4","pages":" 1106-1115"},"PeriodicalIF":4.4,"publicationDate":"2026-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2026/va/d5va00264h?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147621007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of deprivation and socioeconomic factors on inequalities in volatile organic compound emissions in communities in England","authors":"Connor J. Young, Rebecca L. Cordell and Paul S. Monks","doi":"10.1039/D6VA00041J","DOIUrl":"https://doi.org/10.1039/D6VA00041J","url":null,"abstract":"<p >Poor air quality is one of the leading environmental threats to global public health; deprived communities endure disproportionately higher ambient concentrations of primary pollutants such as NO<small>_<em>x</em></small> and PM<small>_2.5</small>. Volatile organic compounds (VOCs) are ubiquitous in the indoor and outdoor environment and can be harmful to human health, yet their relationship with deprivation has not been widely explored. Modelled emissions of VOCs from 2019 across England are linked to indices of multiple deprivation (IMD), land classification, and ethnicity data, to explore the spatial relationship between VOCs, deprivation and social parameters within English communities. VOC emissions are shown to increase as deprivation increases across London and the rest of England, with higher emissions rates in London. Solvent emissions are the dominant source of VOCs across England, and the only source which exhibits a relationship with deprivation. Urban settings demonstrate a higher inequality in emissions in deprived communities, with relative contribution of different sources dependent on geographical location. Population density and minority ethnicity—both proxies for deprivation—correlate with higher VOC emissions, particularly in densely settled minority communities. This research identifies an association between VOC emissions and deprivation in England which has not been seen before. Communities at risk of environmental inequality from VOC emissions are areas of higher ethnic minority populations and increased population density. The relationship with solvent emissions helps to understand the emission sources within these communities, to try to reduce emissions and tackle inequality of air pollution.</p>","PeriodicalId":72941,"journal":{"name":"Environmental science. Advances","volume":" 4","pages":" 1095-1105"},"PeriodicalIF":4.4,"publicationDate":"2026-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2026/va/d6va00041j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147621008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronic PFOA and PFOS exposure triggers cellular oxidative stress and alters lipid levels as revealed through multi-omics analysis","authors":"Jenise Z. Paddayuman, Judith R. Cristobal, Luane J. B. Landau, Ashleigh L. Gagnon, Omer Gokcumen, Diana S. Aga and G. Ekin Atilla-Gokcumen","doi":"10.1039/D5VA00353A","DOIUrl":"10.1039/D5VA00353A","url":null,"abstract":"<p >Per- and polyfluoroalkyl substances (PFAS), or “forever chemicals,” are linked to metabolic, immune, and neurotoxic disorders, yet their long-term cellular effects remain unclear. Using a 24 week chronic exposure model with non-transformed human epithelial cells, we examined responses to low, environmentally relevant concentrations of perfluorooctanoic acid (PFOA) and perfluorooctane sulfonic acid (PFOS). Integrated transcriptomic and lipidomic analyses revealed that despite minimal cellular accumulation, molecular changes emerged around week 17, marked by transcriptional signatures indicative of oxidative stress responses, cell survival pathways, and lipid metabolism. Our data suggests a multi-faceted model in which long-term PFOA and PFOS exposure induces oxidative stress mediated by SESN2 and SOD3, increases lipid biosynthesis <em>via</em> SREBP pathway, and causes compound-specific disruptions of membrane lipids. These findings highlight the importance of multi-omic, time-resolved approaches in uncovering the mechanisms of chronic low-dose chemical exposure and provide a foundation for future <em>in vivo</em> studies.</p>","PeriodicalId":72941,"journal":{"name":"Environmental science. Advances","volume":" 4","pages":" 1081-1094"},"PeriodicalIF":4.4,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12951838/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147349898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}