Engineering biology最新文献_第2页

Programmable cancer treatments: Engineering biology approaches for living cures 可编程癌症治疗：活体疗法的工程生物学方法

Engineering biology Pub Date : 2024-05-31 DOI: 10.1049/enb2.12032

Marc Biarnes Carrera, Alexandra Sevko, Nicholas Glanville, Livija Deban

引用次数: 0

Analysing responsible innovation along a value chain—A single-cell protein case study 分析价值链上的责任创新--单细胞蛋白质案例研究

Engineering biology Pub Date : 2024-03-20 DOI: 10.1049/enb2.12031

Monica Hoyos Flight, Joyce Tait, Theo Chronopoulos, Monica Betancor, Pauline Wischhusen, Emily Burton, Helen Masey O'Neill, Kim van der Heul, John Hays, Peter Rowe

{"title":"Analysing responsible innovation along a value chain—A single-cell protein case study","authors":"Monica Hoyos Flight, Joyce Tait, Theo Chronopoulos, Monica Betancor, Pauline Wischhusen, Emily Burton, Helen Masey O'Neill, Kim van der Heul, John Hays, Peter Rowe","doi":"10.1049/enb2.12031","DOIUrl":"10.1049/enb2.12031","url":null,"abstract":"The British Standards Institution's Publicly Available Specification 440 (PAS 440) provides a Responsible Innovation Framework (RIF) that companies can use to continuously monitor the societal, environmental and health benefits and risks of their innovations, as well as relevant changes to the supply chain and regulations. PAS 440 is intended to help companies achieve the benefits of innovation in a timely manner and avoid any potential harm or unintended misuse of a new product, process or service. Here, the authors have applied the PAS 440 RIF to a novel single-cell protein (SCP) animal feed ingredient taking into consideration the perspectives of the value chain partners (VCPs), companies and laboratories involved in an Innovate UK research project. The authors’ findings show how VCPs can use PAS440 to demonstrate that they are innovating responsibly. Using this approach to responsible innovation along the value chain—from manufacturing scale-up, through regulatory approval, to incorporation in animal feed and from there to food on supermarket shelves—can support the development of innovations that contribute to the economic and environmental sustainability of the animal feed sector. The authors conclude that the PAS 440 Guide can facilitate the progress of a new product throughout a value chain and contribute to coordinating responsible behaviour among companies involved in the value chain.","PeriodicalId":72921,"journal":{"name":"Engineering biology","volume":"8 1","pages":"16-29"},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/enb2.12031","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140192105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Building the bioeconomy: A targeted assessment approach to identifying biobased technologies, challenges and opportunities 建设生物经济：确定生物技术、挑战和机遇的目标评估方法

Engineering biology Pub Date : 2024-02-07 DOI: 10.1049/enb2.12030

Claire Holland, Philip Shapira

{"title":"Building the bioeconomy: A targeted assessment approach to identifying biobased technologies, challenges and opportunities","authors":"Claire Holland, Philip Shapira","doi":"10.1049/enb2.12030","DOIUrl":"10.1049/enb2.12030","url":null,"abstract":"The authors explore opportunities, challenges, and strategies to translate and responsibly scale innovative biobased technologies to build more sustainable bioeconomies. The pandemic and other recent disruptions increased exposure to issues of resilience and regional imbalance, highlighting a need for production and consumption regimes centred more on local biobased resources and dispersed production. The authors review potential biobased technology strategies and identify promising and feasible options for the United Kingdom. Initial landscape and bibliometric analysis identified 50 potential existing and emerging biobased technologies, which were assessed for their ability to fulfil requirements related to biobased production, national applicability, and economic-, societal-, and environmental-benefits, leading to identification of 18 promising biobased production technologies. Further analysis and focus-group discussion with industrial, governmental, academic, agricultural, and social stakeholders, identified three technology clusters for targeted assessment, drawing on cellulose-, lignin-, and seaweed feedstocks. Case studies were developed for each cluster, addressing conversations around sustainable management, use of biomass feedstocks, and associated environmental-, social-, and economic challenges. Cases are presented with discussion of insights and implications for policy. The approach presented is put forward as a scalable assessment method that can be useful in prompting, informing, and advancing discussion and deliberation on opportunities and challenges for biobased transformations.","PeriodicalId":72921,"journal":{"name":"Engineering biology","volume":"8 1","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1049/enb2.12030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139856540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Building the bioeconomy: A targeted assessment approach to identifying biobased technologies, challenges and opportunities 建设生物经济：确定生物技术、挑战和机遇的目标评估方法

Engineering biology Pub Date : 2024-02-07 DOI: 10.1049/enb2.12030

Claire Holland, Philip Shapira

{"title":"Building the bioeconomy: A targeted assessment approach to identifying biobased technologies, challenges and opportunities","authors":"Claire Holland, Philip Shapira","doi":"10.1049/enb2.12030","DOIUrl":"https://doi.org/10.1049/enb2.12030","url":null,"abstract":"The authors explore opportunities, challenges, and strategies to translate and responsibly scale innovative biobased technologies to build more sustainable bioeconomies. The pandemic and other recent disruptions increased exposure to issues of resilience and regional imbalance, highlighting a need for production and consumption regimes centred more on local biobased resources and dispersed production. The authors review potential biobased technology strategies and identify promising and feasible options for the United Kingdom. Initial landscape and bibliometric analysis identified 50 potential existing and emerging biobased technologies, which were assessed for their ability to fulfil requirements related to biobased production, national applicability, and economic‐, societal‐, and environmental‐benefits, leading to identification of 18 promising biobased production technologies. Further analysis and focus‐group discussion with industrial, governmental, academic, agricultural, and social stakeholders, identified three technology clusters for targeted assessment, drawing on cellulose‐, lignin‐, and seaweed feedstocks. Case studies were developed for each cluster, addressing conversations around sustainable management, use of biomass feedstocks, and associated environmental‐, social‐, and economic challenges. Cases are presented with discussion of insights and implications for policy. The approach presented is put forward as a scalable assessment method that can be useful in prompting, informing, and advancing discussion and deliberation on opportunities and challenges for biobased transformations.","PeriodicalId":72921,"journal":{"name":"Engineering biology","volume":"61 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139796916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein engineering in the computational age: An open source framework for exploring mutational landscapes in silico 计算时代的蛋白质工程：探索硅学突变景观的开源框架

Engineering biology Pub Date : 2023-12-07 DOI: 10.1049/enb2.12028

Shirin Bamezai, Giovanni Maresca di Serracapriola, Freya Morris, Rasmus Hildebrandt, Marc Augustine Sojerido Amil, Sporadicate iGEM Team, Rodrigo Ledesma-Amaro

{"title":"Protein engineering in the computational age: An open source framework for exploring mutational landscapes in silico","authors":"Shirin Bamezai, Giovanni Maresca di Serracapriola, Freya Morris, Rasmus Hildebrandt, Marc Augustine Sojerido Amil, Sporadicate iGEM Team, Rodrigo Ledesma-Amaro","doi":"10.1049/enb2.12028","DOIUrl":"https://doi.org/10.1049/enb2.12028","url":null,"abstract":"The field of protein engineering has seen tremendous expansion in the last decade, with researchers developing novel proteins with specialised functionalities for a range of uses, from drug discovery to industrial biotechnology. The emergence of computational tools and high-throughput screening technology has substantially sped up the process of protein engineering. However, much of the expertise required to engage in such projects is still concentrated in the hands of a few specialised individuals, including computational biologists and structural biochemists. The international Genetically Engineered Machine (iGEM) competition represents a platform for undergraduate students to innovate in synthetic biology. Yet, due to their complexity, arduous protein engineering projects are hindered by the resources available and strict timelines of the competition. The authors highlight how the 2022 iGEM Team, ‘Sporadicate’, set out to develop InFinity 1.0, a computational framework for increased accessibility to effective protein engineering, hoping to increase awareness and accessibility to novel in silico tools.","PeriodicalId":72921,"journal":{"name":"Engineering biology","volume":"7 1-4","pages":"29-38"},"PeriodicalIF":0.0,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ietresearch.onlinelibrary.wiley.com/doi/epdf/10.1049/enb2.12028","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138578163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Designing of an extract production protocol for industrial application of cell-free protein synthesis technology: Building from a current best practice to a quality by design approach 为无细胞蛋白质合成技术的工业应用设计提取物生产方案：从当前最佳实践到设计质量方法

Engineering biology Pub Date : 2023-12-06 DOI: 10.1049/enb2.12029

Beatrice Judith Melinek, Jade Tuck, Philip Probert, Harvey Branton, Daniel G. Bracewell

{"title":"Designing of an extract production protocol for industrial application of cell-free protein synthesis technology: Building from a current best practice to a quality by design approach","authors":"Beatrice Judith Melinek, Jade Tuck, Philip Probert, Harvey Branton, Daniel G. Bracewell","doi":"10.1049/enb2.12029","DOIUrl":"https://doi.org/10.1049/enb2.12029","url":null,"abstract":"Cell-Free Protein Synthesis (CFPS) has, over the past decade, seen a substantial increase in interest from both academia and industry. Applications range from fundamental research, through high-throughput screening to niche manufacture of therapeutic products. This review/perspective focuses on Quality Control in CFPS. The importance and difficulty of measuring the Raw Material Attributes (RMAs) of whole cell extract, such as constituent protein and metabolite concentrations, and of understanding and controlling these complicated enzymatic reactions is explored, for both centralised and distributed industrial production of biotherapeutics. It is suggested that a robust cell-free extract production process should produce cell extract of consistent quality; however, demonstrating this is challenging without a full understanding of the RMAs and their interaction with reaction conditions and product. Lack of technology transfer and knowledge sharing is identified as a key limiting factor in the development of CFPS. The article draws upon the experiences of industrial process specialists, discussions within the Future Targeted Healthcare Manufacturing Hub Specialist Working Groups and evidence drawn from various sources to identify sources of process variation and to propose an initial guide towards systematisation of CFPS process development and reporting. These proposals include the development of small scale screening tools, consistent reporting of selected process parameters and analytics and application of industrial thinking and manufacturability to protocol development.","PeriodicalId":72921,"journal":{"name":"Engineering biology","volume":"7 1-4","pages":"1-17"},"PeriodicalIF":0.0,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ietresearch.onlinelibrary.wiley.com/doi/epdf/10.1049/enb2.12029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138578183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Israel and the global synthetic biology ecosystem 以色列与全球合成生物学生态系统

Engineering biology Pub Date : 2023-10-14 DOI: 10.1049/enb2.12027

Yuval Dorfan, Aviv Zeevi, Gita Reinitz, Magi Mualem, Yosi Shacham-Diamand

引用次数: 0

The roadmap of bioeconomy in China 中国生物经济发展路线图

Engineering biology Pub Date : 2022-11-30 DOI: 10.1049/enb2.12026

Xu Zhang, Cuihuan Zhao, Ming-Wei Shao, Yi-Ling Chen, Puyuan Liu, Guo-Qiang Chen

引用次数: 3

Prediction of strain engineerings that amplify recombinant protein secretion through the machine learning approach MaLPHAS 预测通过机器学习方法MaLPHAS扩增重组蛋白分泌的菌株工程。

Engineering biology Pub Date : 2022-09-16 DOI: 10.1049/enb2.12025

Evgenia A. Markova, Rachel E. Shaw, Christopher R. Reynolds

{"title":"Prediction of strain engineerings that amplify recombinant protein secretion through the machine learning approach MaLPHAS","authors":"Evgenia A. Markova, Rachel E. Shaw, Christopher R. Reynolds","doi":"10.1049/enb2.12025","DOIUrl":"10.1049/enb2.12025","url":null,"abstract":"Abstract This article presents a discussion of the process of precision fermentation (PF), describing the history of the space, the expected 70% growth over the next 5 years, various applications of precision fermented products, and the markets available to be disrupted by the technology. A range of prokaryotic and eukaryotic host organisms used for PF are described, with the advantages, disadvantages and applications of each. The process of setting up PF and strain engineering is described, as well as various ways that computational analysis and design techniques can be employed to assist PF engineering. The article then describes the design and implementation of a machine learning method, machine learning predictions having amplified secretion (MaLPHAS) to predict strain engineerings, which optimise the secretion of a recombinant protein. This approach showed an in silico cross‐validated R 2 accuracy on the training data of up to 46.6% and in an in vitro test on a Komagataella phaffii strain, identified one gene engineering out of five predicted, which was shown to double the secretion of a heterologous protein and outperform three of the best‐known edits from the literature for improving secretion in K. phaffii.","PeriodicalId":72921,"journal":{"name":"Engineering biology","volume":"6 4","pages":"82-90"},"PeriodicalIF":0.0,"publicationDate":"2022-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a4/85/ENB2-6-82.PMC9995161.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9197264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Corrigendum: Biologically engineered microbes for bioremediation of electronic waste: Wayposts, challenges and future directions 勘误:用于电子废物生物修复的生物工程微生物:路标、挑战和未来方向

Engineering biology Pub Date : 2022-08-23 DOI: 10.1049/enb2.12023

引用次数: 0