L. Wischerath, A. Rodloff, H. Jentsch, Paul Vielkind, C. Stingu
{"title":"Clonal Relationship within Two Oral Actinomyces Populations Collected from Plaque of Periodontitis Patients","authors":"L. Wischerath, A. Rodloff, H. Jentsch, Paul Vielkind, C. Stingu","doi":"10.31487/J.DOBCR.2021.02.03","DOIUrl":"https://doi.org/10.31487/J.DOBCR.2021.02.03","url":null,"abstract":"Actinomyces naeslundii and A. oris are dental plaque formers involved in the pathogenesis of periodontitis. The aim of the study was to investigate the clonal relationship within two oral Actinomyces populations collected from plaque of patients with chronic periodontitis. The 223 clinical strains of A. naeslundii and A. oris were isolated from biofilm samples collected supra and subgingivally from teeth with shallow (probing depth (PD) = 3-4 mm), deep (PD = 5-6 mm) and very deep (PD ≥7 mm) pockets from 20 chronic periodontitis patients. All strains were submitted to repetitive sequence-based PCR typing using DiversiLab (BioMerieux,Marcy l´Étoile, France). Seven patients harboured only unrelated (<95% similarity) multiple isolates, while 13 harboured both similar (>95% similarity) and unrelated isolates at different sites. Identical (>98% similarity) strains were found to be present in the subgingival shallow depths more often than in the other subgingival depths. The number of clones in individual patients varied from 2 to 17 different rep-PCR genotypes. The clonal relationship within the oral populations of A. naeslundii and A. oris in an individual was unpredictable, ranging from the presence of multiple genotypes with no clonal similarity to only two different clones supra or subgingivally at different sites.","PeriodicalId":72781,"journal":{"name":"Dental Oral Biology and Craniofacial Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48542444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Chowdhury, S. Khijmatgar, M. Mustak, A. Chowdhury, P. DivyaKumari, E. Lynch, M. Grootveld
{"title":"No Association was Detected between Polymorphism of COL1A2 Genes and Occurrence of Dental Fluorosis Among the Subjects Living in a Fluorosis-Endemic Area of India","authors":"C. Chowdhury, S. Khijmatgar, M. Mustak, A. Chowdhury, P. DivyaKumari, E. Lynch, M. Grootveld","doi":"10.31487/J.DOBCR.2020.02.04","DOIUrl":"https://doi.org/10.31487/J.DOBCR.2020.02.04","url":null,"abstract":"Objective: To determine any genetic association of COL1A2 polymorphism and the occurrence of dental fluorosis within an Indian human dental fluorosis population.\u0000Material and Methods: Fifty-six (56) subjects from two groups i.e. cases with dental fluorosis from the Pavagada population (n=29) and a control group (n=27) without fluorosis, were explored. The ages ranged between 15 and 76 years (mean 50.8 years) were included, and the male to female ratio was 70:30. The severity of dental fluorosis was graded using WHO’s Thylstrup-Fejerskov index (TF), and the concentration of fluoride was determined by a fluoride ion selective electrode (ISE). Genomic DNA was extracted using the standard phenol-chloroform method. The rs412777 and rs414408 polymorphism in COL1A2 were genotyped using the Sanger sequence method.\u0000Results: Genotype distributions for rs412777 within each group were: AA 41%, AC 51%, and CC 7% for dental fluorosis participants, and AA 56%, AC 46%, and CC 0% for the control participants.\u0000Conclusions: The rs412777 and rs414408 polymorphisms in the COL1A2gene showed no significant association between COL1A2 and the occurrence of dental fluorosis amongst this Indian population.","PeriodicalId":72781,"journal":{"name":"Dental Oral Biology and Craniofacial Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46287651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Risk Factors of Early Dental Implant Failure: A Retrospective Study","authors":"Ha Nguyen Thi Khanh, M. Duc, T. T. Xuan","doi":"10.31487/J.DOBCR.2020.04.08","DOIUrl":"https://doi.org/10.31487/J.DOBCR.2020.04.08","url":null,"abstract":"Objective: This retrospective study aimed to assess the risk factors associated with early implant failure using a multivariate logistic regressive model and generalized evaluating equations (GEE) analysis. A total of 892 patients who received 1931 dental implants within the period from 2015 to 2018 at Odonto-Maxillo-Facial Hospital, Ho Chi Minh City, Vietnam were included in this study. Several factors as age, gender, implant placement site, implant length and diameter, sinus lifting technique, the number of implants per patient, and implant brands were recorded. GEE analysis results showed that the implant-based failure rates were 6.1% after an observation period from 3 months to 3 years. Among 118 failed implants, 108 implants were lost before or at the abutment connection stage (91.5%). Male, maxilla implant placement and implant brands were identified as the risk factors for early implant loss. Implant brands which have significantly less early failure rates are Ritter, Nobel, MIS, Osstem, Neo.","PeriodicalId":72781,"journal":{"name":"Dental Oral Biology and Craniofacial Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49278948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Chakraborty, Honghua Hu, A. Mangani, K. Vickery, S. Ranganathan
{"title":"Combined Bacterial Antigen Lipopolysaccharide and Lipoteichoic Acid Increase Cal 27 Oral Cancer Cell Proliferation","authors":"R. Chakraborty, Honghua Hu, A. Mangani, K. Vickery, S. Ranganathan","doi":"10.31487/J.DOBCR.2021.01.03","DOIUrl":"https://doi.org/10.31487/J.DOBCR.2021.01.03","url":null,"abstract":"Oral biofilms harbour gram-negative bacterial antigen lipopolysaccharide (LPS) involved in oral cancer\u0000progression and gram-positive bacterial surface-associated adhesive, lipoteichoic acid (LTA). Thus, we\u0000hypothesised that LPS and LTA together would increase the proliferation of cancer cells compared to\u0000stimulation by LPS alone. Oral cancer cell lines SCC4, SCC9, SCC25, Cal 27 and the normal oral cell line,\u0000OKF6, were studied. The bacterial antigen stimulation indices were determined using the MT Glo assay.\u0000Cell proliferation after bacterial antigen stimulation was validated by clonogenic assays. Phosphokinase\u0000array, reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and Western blot were\u0000employed to study proliferative and apoptotic pathways in bacterial antigen-stimulated cells. Bacterial\u0000antigens significantly stimulated Cal 27 (p ≤ 0.001) alone. SCC4 and SCC9 showed negligible stimulation\u0000with either antigen, while SCC25 results were comparable to OKF6. The combined antigen stimulation of\u0000Cal 27 led to a decrease in phosphorylated p53 and β-catenin and higher PI3K compared to LPS only\u0000stimulated cells (p ≤ 0.001). Combined bacterial antigen stimulation results in increased proliferation of Cal\u000027 cells due to lowering of tumor suppressor proteins and increased tumor proliferation-related proteins.","PeriodicalId":72781,"journal":{"name":"Dental Oral Biology and Craniofacial Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42536958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Human and Animal Studies in Craniofacial Embryology Enrich Human Postnatal Craniofacial Insight Differently","authors":"I. Kjaer","doi":"10.31487/J.DOBCR.2021.01.02","DOIUrl":"https://doi.org/10.31487/J.DOBCR.2021.01.02","url":null,"abstract":"Objectives: To compare results obtained since 1970 from craniofacial embryological studies on human\u0000fetal tissues with results from similar studies performed on animal tissues and focus on how the different\u0000tissue types and methods can enrich the human postnatal craniofacial research. There are three sections: i)\u0000research on normal and pathological human fetal material, ii) animal experimental research performed on\u0000different species, and iii) comparisons of the results obtained.\u0000Human Material: On human prenatal material, normal and pathological developmental processes in the\u0000mandible, maxilla, nasal cavity, body axis, cranial base, vomeronasal organs, pituitary gland and nervous\u0000system were related to postnatal findings. Specific focus was given to pre/postnatal bridging of observations\u0000on holoprosencephaly, cleft lip/palate, Down syndrome, myelomeningocele/spina bifida, Kallmann\u0000syndrome, Turner syndrome and Fragile X syndrome. Demarcations of pathological regions in Cri du chat,\u0000Velo-cardio-Facial syndrome and Crouzon syndrome were mapped.\u0000Animal Material: Animal experimental results from studies on the notochord, gastrulation, neural crest,\u0000hindbrain, rhombomere, homeobox genes and experimentally induced malformation were presented.\u0000Comparing materials: The educational background of the scientists performing human and animal\u0000research, their postnatal clinical experience, the research materials used and the methods available for\u0000research are compared. Furthermore, the obtained findings result in a “pro et contra list” indicating what is\u0000positive and what is negative for improving postnatal human diagnostics.\u0000Conclusion: Human and animal studies in craniofacial embryology enrich human postnatal craniofacial\u0000insight differently. Future cooperation between human and animal research cultures is recommended.\u0000","PeriodicalId":72781,"journal":{"name":"Dental Oral Biology and Craniofacial Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43601581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Electrospinning Parameters on the Fiber Diameter and Morphology of PLGA Nanofibers.","authors":"Lohitha Kalluri, Megha Satpathy, Yuanyuan Duan","doi":"10.31487/j.dobcr.2021.02.04","DOIUrl":"https://doi.org/10.31487/j.dobcr.2021.02.04","url":null,"abstract":"<p><strong>Background: </strong>Poly lactic-co-glycolic acid (PLGA) has been widely investigated for various biomedical applications, such as craniofacial bone regeneration, wound dressing and tissue engineering. Electrospinning is a versatile technology used to produce micro/nanoscale fibers with large specific surface area and high porosity.</p><p><strong>Purpose: </strong>The aim of the current study is to prepare PLGA nanofibers using electrospinning for guided tissue regeneration/guided bone regeneration applications. The objective of this study is to determine the appropriate electrospinning parameters such as applied voltage, flow rate, spinneret-collector distance and polymer solution concentration for preparation of PLGA fibrous membrane and their effect on the mean fiber diameter of the electrospun fibers.</p><p><strong>Method: </strong>PLGA pellets were dissolved in Hexafluoroisopropanol (HFIP) in various concentrations overnight using a bench rocker. The resulting PLGA solution was then loaded into a syringe and electrospinning was done by maintaining the other parameters constant. Similarly, various fibrous mats were collected by altering the specific electrospinning parameter inputs such as applied voltage, flow rate and spinneret-collector distance. The morphology of the fibrous mats was characterized using Scanning Electron Microscope. The mean fiber diameter was assessed using ImageJ software and the results were compared using one-way ANOVA.</p><p><strong>Results: </strong>We obtained bead-free uniform fibers with various tested solution concentrations. One-way ANOVA analysis demonstrated significant variation in mean fiber diameter of the electrospun fibers with altering applied voltage, solution concentration, flow rate and spinneret-collector distance.</p><p><strong>Conclusion: </strong>The above-mentioned electrospinning parameters and solution concentration influence the mean fiber diameter of electrospun PLGA nanofibers.</p>","PeriodicalId":72781,"journal":{"name":"Dental Oral Biology and Craniofacial Research","volume":"4 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10035641/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9546604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Plasma Treatment Effects on Oral <i>Candida albicans</i> Biofilms.","authors":"Qing Hong, Xiaoqing Dong, Meng Chen, Hongmin Sun, Liang Hong, Yong Wang, Qingsong Yu","doi":"10.31487/j.dobcr.2021.02.05","DOIUrl":"https://doi.org/10.31487/j.dobcr.2021.02.05","url":null,"abstract":"<p><p>The objective of this study is to evaluate the plasma treatment effects on oral fungal biofilms. <i>Candida albicans</i> biofilms were developed on the 48-well plate to serve as a model of oral fungal biofilm. The treatment of 0.2% chlorhexidine digluconate (CHX) was used as a positive control compared with plasma treatments. The efficacy of treatments was determined by 3-(4,5-dimethylazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and confocal laser scanning microscope (CLSM). The survival percentage of <i>Candida albicans</i> decreased from 52% to 27% as the plasma power increased from 6mA to 8mA and plasma exposure time extended from 2 min to 10 min. Moreover, it was found that there is a synergistic effect of the combination of plasma and CHX treatments. Scanning electron microscopy (SEM) examination indicated severe cell damages resulting from plasma treatment. In conclusion, the low-temperature plasma treatment is effective in deactivating <i>Candida albicans</i> biofilms and thus provides a promising alternative to disinfect oral fungal biofilms.</p>","PeriodicalId":72781,"journal":{"name":"Dental Oral Biology and Craniofacial Research","volume":"4 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9854346/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9993703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Enamel Matrix Derivative and TGF-Beta 1 Target Genes in Human Tongue Carcinoma Cells","authors":"M. Mauramo, Suvi‐Tuuli Vilén, T. Sorsa, T. Salo","doi":"10.31487/J.DOBCR.2020.06.05","DOIUrl":"https://doi.org/10.31487/J.DOBCR.2020.06.05","url":null,"abstract":"Enamel matrix derivative (EMD) can enhance proliferation and migration of different oral cell lines,\u0000including malignant oral carcinoma cells, in vitro and in vivo. The composition of EMD is not known, but\u0000part of the effects have been postulated to be caused by transforming growth factor-beta-1 (TGF-beta 1).\u0000This study aimed to compare target genes of EMD and TGF-beta 1 on highly malignant oral carcinoma\u0000HSC-3 cells. Microarrays were used to examine differentially expressed genes in HSC-3 cells after 6h and\u000024h incubations with EMD (200 µg/ml) or TGF-beta 1 (10 ng/ml). Gene Ontology (GO) enrichment analysis\u0000of the regulated genes was also conducted. After 6h and 24h of EMD treatments 42 and 12 genes,\u0000respectively, were statistically significantly (P<0.05) up- or down-regulated. However, as many as 393 and\u0000346 genes were statistically significantly (P<0.05) up- or down-regulated by TGF-beta 1. Among the most\u0000up-regulated genes by both of the study reagents were MMP-9 and -10. The expression of MMP-10 by\u0000EMD treated carcinoma cells was also verified in protein level. In conclusion, TGF-beta 1 regulates more\u0000and mostly different genes compared with EMD, but both regulate the expression of matrix\u0000metalloproteinase genes in oral carcinoma cells.","PeriodicalId":72781,"journal":{"name":"Dental Oral Biology and Craniofacial Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45053249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sharifah Nabihah Syed Abdurahman, Nik Mohamad Faris Azzimi Nik Zulkifli, S. N. A. Ghafar, S. Abdullah
{"title":"Biofilm Formation between Species Associated with Denture Stomatitis","authors":"Sharifah Nabihah Syed Abdurahman, Nik Mohamad Faris Azzimi Nik Zulkifli, S. N. A. Ghafar, S. Abdullah","doi":"10.31487/J.DOBCR.2020.06.02","DOIUrl":"https://doi.org/10.31487/J.DOBCR.2020.06.02","url":null,"abstract":"Denture stomatitis (DS) can affect about 70% of denture wearers causing inflammation of oral mucosa\u0000underneath the removable denture mostly seen at the palatal area. Candida albicans is a well-known as the\u0000main causative agent associated with denture stomatitis. Studies shown that DS-related biofilm consists of\u0000complex structured of microbial communities. Presence of co-existing bacteria in these biofilms may also\u0000contribute to the infection. In order to understand the microbial interaction between C. albicans and bacteria,\u0000this study focus on the ability of bacteria species which are Streptococcus mutans and Staphylococcus\u0000aureus to form biofilm with and without the presence of C. albicans. For this study, two groups were\u0000observed which are the single species biofilm and the mixed species biofilm. Biofilm assay was utilized to\u0000measure biofilm mass using spectrophotometer. From the observation, single species groups formed a better\u0000biofilm when compared to fungal-bacterial biofilm. Even though biofilm assay is unable to determine the\u0000number of cell available, both species were present when fungal-bacterial biofilm sample was observed\u0000microscopically using Gram stain technique. This experiment showed that both fungal and bacteria species\u0000are able to form biofilm together and this finding can be investigated further for better understanding of DSrelated biofilm.","PeriodicalId":72781,"journal":{"name":"Dental Oral Biology and Craniofacial Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42191448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Mariano, Mendoza Cuello Norbin José, B. Alejandro, Attaguile Alberto, Giannunzio Graciela Ana
{"title":"Three-Dimensional Biomodels: Use of Different Materials for the Surgical Planning in Relation to Two Mandibular Cysts Cases","authors":"A. Mariano, Mendoza Cuello Norbin José, B. Alejandro, Attaguile Alberto, Giannunzio Graciela Ana","doi":"10.31487/j.dobcr.2020.05.01","DOIUrl":"https://doi.org/10.31487/j.dobcr.2020.05.01","url":null,"abstract":"Introduction: The use of three-dimensional models in the diagnosis, planification and treatment of the\u0000maxillofacial area pathology has been included in publications since 1980, and has enabled to minimize\u0000surgical time, reducing peri and postoperative morbidity, and optimizing surgical results. Currently, the\u0000market offers a huge range of 3D printers and materials which allows biomodels to be printed for medical\u0000purposes. The purpose of the following paper is to describe the use of two types of three-dimensional models\u0000in the therapeutic planning of two cases of mandibular cysts.\u0000Case Report: Two cases of mandibular dentigerous cysts are described, in which osteosynthesis plates were\u0000used to prevent pathological fractures, pre-modeled together with acrylic positioners on three-dimensional\u0000models and printed in different ways. Postoperative clinical and tomographic controls were performed.\u0000Conclusion: The knowledge of the properties of the different materials available for the construction of\u0000three-dimensional models allows selecting the one that best suits the case needs required, minimizing\u0000surgical times and optimizing the results.","PeriodicalId":72781,"journal":{"name":"Dental Oral Biology and Craniofacial Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48048423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}