Advanced techniques in biology & medicine最新文献_第8页

DNA Methylation, Speciation and Domestication DNA甲基化、物种形成和驯化

Advanced techniques in biology & medicine Pub Date : 2017-07-14 DOI: 10.4172/2379-1764.1000234

Z. Banlaki

引用次数: 0

Contribution of DUSP28 to Regulation of Mucins in Human Pancreatic Cancer Cells DUSP28在人胰腺癌细胞粘蛋白调控中的作用

Advanced techniques in biology & medicine Pub Date : 2017-07-14 DOI: 10.4172/2379-1764.1000232

Jungwhoi Lee, Jae Hoon Kim

{"title":"Contribution of DUSP28 to Regulation of Mucins in Human Pancreatic Cancer Cells","authors":"Jungwhoi Lee, Jae Hoon Kim","doi":"10.4172/2379-1764.1000232","DOIUrl":"https://doi.org/10.4172/2379-1764.1000232","url":null,"abstract":"Pancreatic cancer remains one of the most deadly cancers, and once diagnosed, the prognosis for patient survival is poor. Patient outcomes have not been improved despite considerable and continuous efforts. We have suggested that dual-specificity phosphatase 28 (DUSP28) is a potential anti-cancer target to inhibit malignant pancreatic cancers. In this context, atypical DUSP28 can affect the regulation of mucins such as mucin5B (MUC5B) and mucin16 (MUC16). To investigate this correlation, we analysed mRNA levels of DUSP28 and mucins using the Gene Expression Omnibus public microarray database in pancreatic cancer, which indicated higher DUSP28, MUC1, MUC4, MUC5B, MUC16 and MUC20 mRNA levels in pancreatic cancers compared with normal pancreas tissue. In addition, DUSP28 expression in human pancreatic cancers correlated positively with those of MUC1, MUC4, MUC5B, MUC16 and MUC20. In contrast, there were no significant correlations between DUSP28 and mucins in normal pancreas tissues. Decreased DUSP28 expression resulted in down regulation of MUC5B and MUC16 at both the mRNA and protein levels. Furthermore, blockade of MUC5B or MUC16 expression inhibited migration and survival of cancer cells through the inhibition of phosphorylated FAK and ERK1/2. Collectively, we propose that DUSP28 uniquely links regulation of MUC5B and MUC16 to migration and survival of pancreatic cancer cells, which strongly support a rationale for targeting DUSP28 to inhibit development of malignant pancreatic cancer.","PeriodicalId":7277,"journal":{"name":"Advanced techniques in biology & medicine","volume":"65 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2017-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91318700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The PE/PPE Multigene Family of Mycobacteria and TB Vaccines 分枝杆菌PE/PPE多基因家族与结核疫苗

Advanced techniques in biology & medicine Pub Date : 2017-06-29 DOI: 10.4172/2379-1764.1000231

M. Brennan

{"title":"The PE/PPE Multigene Family of Mycobacteria and TB Vaccines","authors":"M. Brennan","doi":"10.4172/2379-1764.1000231","DOIUrl":"https://doi.org/10.4172/2379-1764.1000231","url":null,"abstract":"Volume 5 • Issue 3 • 1000231 Adv Tech Biol Med, an open access journal ISSN: 2379-1764 Mycobacteria including Mycobacteria tuberculosis (Mtb) contain a multi-gene family, named the PE/PPE family. Mycobacterial strains contain about 165 genes, consisting of two major subfamilies the PE (containing the PE_PGRS group) and PPE, of which many of the constituents are very homologous (>50%). Since their discovery, scientists speculated about the role the members in this family might play in Mtb pathogenesis, immunity, evolution, and antigenic variation. Brennan et al. [1] in Issue 6 of Infection and Immunity, summarizes what is known about PE/PPEs with a particular focus on how they could be used in new TB vaccines. In fact, two TB vaccines presently being studied in clinical trials, GSK’s M72 and IDRI’s ID93, each contain a PPE protein. Another TB vaccine, the live Mtb mutant strain MtbΔPPE/ PE25-PE19 developed by the laboratories at the University of Pisa and Institute Pasteur, is a mutant which is missing two PEs and three PPEs, is under preclinical development. Evidence indicates that this vaccine is safe and is protective due to cross reactivity among the PE and PPEs in animal models for TB.","PeriodicalId":7277,"journal":{"name":"Advanced techniques in biology & medicine","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79029237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flattening and Unpacking Human Genetic Variation in Mexico, Postwar to the Present 从战后到现在，墨西哥人类基因变异的扁平化和拆解

Advanced techniques in biology & medicine Pub Date : 2017-06-28 DOI: 10.4172/2379-1764.1000233

Víctor H. Anaya-Muñoz

引用次数: 1

The Zebrafish Model to Study the Role of microRNAs in Glomerular Function and Disease 用斑马鱼模型研究microrna在肾小球功能和疾病中的作用

Advanced techniques in biology & medicine Pub Date : 2017-06-23 DOI: 10.4172/2379-1764.1000230

J. Müller-Deile, M. Schiffer

{"title":"The Zebrafish Model to Study the Role of microRNAs in Glomerular Function and Disease","authors":"J. Müller-Deile, M. Schiffer","doi":"10.4172/2379-1764.1000230","DOIUrl":"https://doi.org/10.4172/2379-1764.1000230","url":null,"abstract":"microRNAs (miRs) are non-coding small RNAs that play an important role in posttranscriptional regulation of gene expression. Recent studies indicate that miRs are also mediators in different disease processes. Here we review how the zebrafish model can be used to study the role of miRs in glomerular function and disease. Microinjections of miR mimics were done in zebrafish eggs and larvae. A transgenic zebrafish line which expresses a green fluorescent plasma protein was used to investigate protein loss though the glomerular filtration barrier. Electron microscopy analysis revealed the level and degree of glomerular damage after miR overexpression. MiR- 143-3p seems to be important for glomerular glycocalyx as overexpression of miR-143-3p leads to down regulation of versican, proteinuria and damage on the endothelial and epithelial side of the glomerular filtration barrier. In contrast, overexpression of miR-378a-3p by injection of a specific miR mimic caused proteinuria, edema, podocyte effacement and thickening of the glomerular basement membrane. These findings could be rescued by coinjections of a nephronectin construct with a mutated 3’UTR region where the miR could not bind. Thus, miR mimics can be used in the zebrafish model to study the role of miRs involved in glomerular diseases.","PeriodicalId":7277,"journal":{"name":"Advanced techniques in biology & medicine","volume":"45 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2017-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85221692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Commentary on Covalent Tethering of Photo-responsive Superficial Layers on Hydrogel Surfaces 水凝胶表面光响应浅层共价系缚研究进展

Advanced techniques in biology & medicine Pub Date : 2017-06-21 DOI: 10.4172/2379-1764.1000229

Lie Chen, Mingjie Liu

引用次数: 0

Ecotoxicological Evaluation of Parallelomorphus laevigatus (Coleoptera, Carabidae) as a Useful Bioindicator of Soil Metal Pollution 作为土壤金属污染有用生物指示物的laevigatus生态毒理学评价(鞘翅目，蜈蚣科

Advanced techniques in biology & medicine Pub Date : 2017-06-20 DOI: 10.4172/2379-1764.1000228

E. Conti

引用次数: 5

Genotype-Phenotype Correlation - Two Families with GCH1 Mutations 基因型-表型相关性-两个GCH1突变家族

Advanced techniques in biology & medicine Pub Date : 2017-05-29 DOI: 10.4172/2379-1764.1000225

Yaping Yan, Bo Zhang

引用次数: 0

The Mycology as Forensics Tool 真菌学作为法医工具

Advanced techniques in biology & medicine Pub Date : 2017-05-29 DOI: 10.4172/2379-1764.1000226

Tranchida María Cecilia, Cabello Marta Noemí

引用次数: 4

Screening for MPN Mutations in Cases of Deep Vein Thrombosis and/or Pulmonary Embolism: What We have learnt from Studies 深静脉血栓和/或肺栓塞病例中MPN突变的筛查:我们从研究中学到的东西

Advanced techniques in biology & medicine Pub Date : 2017-05-22 DOI: 10.4172/2379-1764.1000224

J. Ianotto

{"title":"Screening for MPN Mutations in Cases of Deep Vein Thrombosis and/or Pulmonary Embolism: What We have learnt from Studies","authors":"J. Ianotto","doi":"10.4172/2379-1764.1000224","DOIUrl":"https://doi.org/10.4172/2379-1764.1000224","url":null,"abstract":"Myeloproliferative neoplasms (MPN) are chronic myeloid disorders characterized by a high-risk of thrombosis. One-third is in venous vessels. Clinicians who treat patients experiencing thromboses in such vessels know the high rate of cancer in such situation. Many studies have been published concerning the screening for mutations that drive MPNs (mostly JAK2V617F and CALR mutations) in case of deep vein thromboses and/or pulmonary embolism. We reviewed the results of the studies published since 2005 (year of discovery of JAK2V617F, the most frequent of these mutations) and we analyzed the prevalence of mutations among the patients and their characteristics. Sixteen studies have been published on this topic. Of 2907 patients, 39 (1.3%) were positive for JAK2V617F, reaching 2.1% in case of history of recurrence. CALR mutations have not been found in any of the studied situations. Women represent 73.5% of the cases. Patients over the age of 60 account for 76.5% of the cases. Only 10 (29.4%) of the patients have been identified to have MPN despite a median follow-up period of 42 months. All had thrombocytosis or polycythemia at the time of the thrombosis. Nineteen patients experienced thrombotic recurrence, describing JAK2V617F mutation as a pro-thrombotic factor. Screening for JAK2V617F or CALR mutations should not be systematically performed for patients experiencing deep vein thromboses and/or pulmonary embolism because of the low rate of positivity. Attention should perhaps be focused on patients with persistent thrombocytosis or polycythemia who have a higher rate of MPNs. For the other positive cases with no features of MPN, the management is unclear, but a thorough evaluation by a hematologist should be performed, and the patients should be followed for years.","PeriodicalId":7277,"journal":{"name":"Advanced techniques in biology & medicine","volume":"70 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2017-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74170297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0