Current protocols in toxicology最新文献_第8页

Use of Human Pluripotent Stem Cell Derived-Cardiomyocytes to Study Drug-Induced Cardiotoxicity. 利用人多能干细胞衍生心肌细胞研究药物诱导的心脏毒性。

Current protocols in toxicology Pub Date : 2017-08-04 DOI: 10.1002/cptx.30

Agnes Maillet, Kim Peng Tan, Liam R Brunham

引用次数: 3

3D Differentiation of LUHMES Cell Line to Study Recovery and Delayed Neurotoxic Effects. 三维分化 LUHMES 细胞系，研究恢复和延迟神经毒性效应。

Current protocols in toxicology Pub Date : 2017-08-04 DOI: 10.1002/cptx.29

Georgina Harris, Helena Hogberg, Thomas Hartung, Lena Smirnova

引用次数: 0

Combining Cytotoxicity Assessment and Xenopus laevis Phenotypic Abnormality Assay as a Predictor of Nanomaterial Safety. 结合细胞毒性评估和非洲爪蟾表型异常测定作为纳米材料安全性的预测因子。

Current protocols in toxicology Pub Date : 2017-08-04 DOI: 10.1002/cptx.25

Karamallah Al-Yousuf, Carl A Webster, Grant N Wheeler, Francesca Baldelli Bombelli, Victoria Sherwood

{"title":"Combining Cytotoxicity Assessment and Xenopus laevis Phenotypic Abnormality Assay as a Predictor of Nanomaterial Safety.","authors":"Karamallah Al-Yousuf, Carl A Webster, Grant N Wheeler, Francesca Baldelli Bombelli, Victoria Sherwood","doi":"10.1002/cptx.25","DOIUrl":"https://doi.org/10.1002/cptx.25","url":null,"abstract":"The African clawed frog, Xenopus laevis, has been used as an efficient pre-clinical screening tool to predict drug safety during the early stages of the drug discovery process. X. laevis is a relatively inexpensive model that can be used in whole organism high-throughput assays whilst maintaining a high degree of homology to the higher vertebrate models often used in scientific research. Despite an ever-increasing volume of biomedical nanoparticles (NPs) in development, their unique physico-chemical properties challenge the use of standard toxicology assays. Here, we present a protocol that directly compares the sensitivity of X. laevis development as a tool to assess potential NP toxicity by observation of embryo phenotypic abnormalities/lethality after NP exposure, to in vitro cytotoxicity obtained using mammalian cell lines. In combination with conventional cytotoxicity assays, the X. laevis phenotypic assay provides accurate data to efficiently assess the safety of novel biomedical NPs. © 2017 by John Wiley & Sons, Inc.","PeriodicalId":72743,"journal":{"name":"Current protocols in toxicology","volume":"73 ","pages":"20.13.1-20.13.33"},"PeriodicalIF":0.0,"publicationDate":"2017-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cptx.25","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35294102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Intracellular Cytokine Detection by Flow Cytometry in Surface Marker-Defined Human Peripheral Blood Mononuclear T Cells. 流式细胞术检测表面标记的人外周血单个核T细胞的细胞内细胞因子。

Current protocols in toxicology Pub Date : 2017-08-04 DOI: 10.1002/cptx.26

Fredine T Lauer, Jesse L Denson, Ellen Beswick, Scott W Burchiel

{"title":"Intracellular Cytokine Detection by Flow Cytometry in Surface Marker-Defined Human Peripheral Blood Mononuclear T Cells.","authors":"Fredine T Lauer, Jesse L Denson, Ellen Beswick, Scott W Burchiel","doi":"10.1002/cptx.26","DOIUrl":"https://doi.org/10.1002/cptx.26","url":null,"abstract":"In a recent unit in this series, protocols for the isolation, cryopreservation, thawing, and immunophenotyping of HPBMC isolated from peripheral whole blood using cell surface marker (CSM) staining and multi-color flow cytometry analysis were presented. The current procedure describes the detection and quantification of CSM and intracellular markers (ICM), including transcription factors and cytokines, following activation and differentiation of CD4+ T-cells using multi-color flow cytometry. Results indicated that repeatable and robust detection of ICM could be obtained in surface marker-defined T cells that identify functional subsets of cells. There were no observed differences between fresh and cryopreserved HPBMC in eight phenotypes analyzed (T-CD3, Th-CD4, Tmem-CD45RO, activated T-CD3/CD25, Treg- Foxp3/CD25, Th1-IFNγ, Th2- IL-4, Th17-IL-17A). There was an observed difference in activated T- CD3/CD69 in the short term (30-90 days) cryopreserved samples as compared to the freshly isolated samples, which may have resulted from the variance in controls or small sample size. © 2017 by John Wiley & Sons, Inc.","PeriodicalId":72743,"journal":{"name":"Current protocols in toxicology","volume":"73 ","pages":"18.19.1-18.19.14"},"PeriodicalIF":0.0,"publicationDate":"2017-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cptx.26","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35296075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Use of Primary Rat Hepatocytes for Prediction of Drug-Induced Mitochondrial Dysfunction 用原代大鼠肝细胞预测药物性线粒体功能障碍

Current protocols in toxicology Pub Date : 2017-05-02 DOI: 10.1002/cptx.24

Cong Liu, Shuichi Sekine, Binbin Song, Kousei Ito

{"title":"Use of Primary Rat Hepatocytes for Prediction of Drug-Induced Mitochondrial Dysfunction","authors":"Cong Liu, Shuichi Sekine, Binbin Song, Kousei Ito","doi":"10.1002/cptx.24","DOIUrl":"10.1002/cptx.24","url":null,"abstract":"Mitochondrial dysfunction plays a central role in drug-induced liver injury. To evaluate drug-induced mitochondrial impairment, several isolated mitochondria- or cell line-based assays have been reported. Among them, culturing HepG2 cells in galactose provides a remarkable method to assess mitochondrial toxicity by activating mitochondrial aerobic respiration. We applied this assay to primary rat hepatocytes by culturing cells in galactose and hyperoxia to enhance the evaluation of metabolism-related drug-induced mitochondrial toxicity. Conventional culture of primary hepatocytes under high-glucose and hypoxic conditions could force cells to switch energy generation to glycolysis. By contrast, cells cultured in galactose and hyperoxia could maintain energy generation from mitochondrial aerobic respiration, which is consistent with physiological conditions, and consequently improve the susceptibility of cells to mitochondrial toxicants. Measuring the toxicities of test compounds in primary rat hepatocytes cultured in modified conditions provides a useful model to identify mitochondrial dysfunction-mediated drug-induced hepatotoxicity. © 2017 by John Wiley & Sons, Inc.","PeriodicalId":72743,"journal":{"name":"Current protocols in toxicology","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cptx.24","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34959897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Micropatterned Co-Cultures of Human Hepatocytes and Stromal Cells for the Assessment of Drug Clearance and Drug-Drug Interactions 人肝细胞和基质细胞微模式共培养用于评估药物清除和药物-药物相互作用

Current protocols in toxicology Pub Date : 2017-05-02 DOI: 10.1002/cptx.23

Christine Lin, Salman R Khetani

引用次数: 13

Quantification of Lactate Dehydrogenase for Cell Viability Testing Using Cell Lines and Primary Cultured Astrocytes 乳酸脱氢酶在细胞系和原代培养星形胶质细胞细胞活力检测中的定量测定

Current protocols in toxicology Pub Date : 2017-05-02 DOI: 10.1002/cptx.21

Simon Kaja, Andrew J. Payne, Yuliya Naumchuk, Peter Koulen

引用次数: 87

Flow-Cytometry-Based Method to Detect Escherichia coli and Shigella Spp. Using 16S rRNA-Based Probe 基于流式细胞术的16S rrna探针检测大肠杆菌和志贺氏菌的方法

Current protocols in toxicology Pub Date : 2017-02-01 DOI: 10.1002/cptx.14

Yong Xue, Jon G. Wilkes, Ted J. Moskal, Anna J. Williams, Willie M. Cooper, Dan A. Buzatu

引用次数: 1

Detecting Protein Sulfenylation in Cells Exposed to a Toxicant 检测暴露于毒物的细胞中的蛋白质亚砜化

Current protocols in toxicology Pub Date : 2017-02-01 DOI: 10.1002/cptx.16

Phillip A. Wages

引用次数: 0

Detection and Manipulation of the Stress Response Protein Metallothionein 应激反应蛋白金属硫蛋白的检测与调控

Current protocols in toxicology Pub Date : 2017-02-01 DOI: 10.1002/cptx.17

Sadikshya Bhandari, Clare Melchiorre, Kristen Dostie, Debby Laukens, Lindsey Devisscher, Ariel Louwrier, Amy Thees, Michael A. Lynes

{"title":"Detection and Manipulation of the Stress Response Protein Metallothionein","authors":"Sadikshya Bhandari, Clare Melchiorre, Kristen Dostie, Debby Laukens, Lindsey Devisscher, Ariel Louwrier, Amy Thees, Michael A. Lynes","doi":"10.1002/cptx.17","DOIUrl":"10.1002/cptx.17","url":null,"abstract":"Metallothioneins (MTs) are small molecular weight stress response proteins that play a central role as reservoir of essential divalent heavy metal cations such as zinc and copper, and also can diminish the effects of toxic heavy metals such as mercury and cadmium. Historically, MT has been considered to be an intracellular protein with roles to play in the management of heavy metals, as a regulator of cellular redox potential, and as a buffer of free radicals. Our recent studies have highlighted immunomodulatory role of MT in inflammatory diseases and also in the progression of metastatic cell movement. Hence, manipulation and detection of MT is essential for its possible use as a diagnostic and in therapeutic interventions of chronic inflammation. This review describes procedures used to detect MT using techniques such as western immunoblot, competition ELISA, flow cytometry and immunohistochemistry. Additionally, it also describes the use of a colorimetric cell proliferation assay (CellTiter 96 AQueous One Solution/MTS) to study the proliferative effect of MT. © 2017 by John Wiley & Sons, Inc.","PeriodicalId":72743,"journal":{"name":"Current protocols in toxicology","volume":"71 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cptx.17","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46881842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12