Clinical and diagnostic laboratory immunology最新文献

{"title":"Immunogenicity of an intranasally administered modified live canine parvovirus type 2b vaccine in pups with maternally derived antibodies.","authors":"Vito Martella,&nbsp;Alessandra Cavalli,&nbsp;Nicola Decaro,&nbsp;Gabriella Elia,&nbsp;Costantina Desario,&nbsp;Marco Campolo,&nbsp;Giancarlo Bozzo,&nbsp;Elvira Tarsitano,&nbsp;Canio Buonavoglia","doi":"10.1128/CDLI.12.10.1243-1245.2005","DOIUrl":"https://doi.org/10.1128/CDLI.12.10.1243-1245.2005","url":null,"abstract":"<p><p>The ability of a modified live canine parvovirus type 2b vaccine to elicit active immunization in pups with maternally derived antibodies (MDA) by intranasal administration was evaluated. The vaccine induced seroconversion in 100% of pups with MDA titers of < or = 80 and in 51.6% of pups with titers between 160 and 320.</p>","PeriodicalId":72602,"journal":{"name":"Clinical and diagnostic laboratory immunology","volume":"12 10","pages":"1243-5"},"PeriodicalIF":0.0,"publicationDate":"2005-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/CDLI.12.10.1243-1245.2005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25624938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitivity and specificity of a monoclonal antibody-based fluorescence assay for detecting Enterocytozoon bieneusi spores in feces of simian immunodeficiency virus-infected macaques.","authors":"Inderpal Singh,&nbsp;Abhineet S Sheoran,&nbsp;Quanshun Zhang,&nbsp;Angela Carville,&nbsp;Saul Tzipori","doi":"10.1128/CDLI.12.10.1141-1144.2005","DOIUrl":"https://doi.org/10.1128/CDLI.12.10.1141-1144.2005","url":null,"abstract":"<p><p>Enterocytozoon bieneusi is clinically the most significant among the microsporidia causing chronic diarrhea, wasting, and cholangitis in individuals with human immunodeficiency virus/AIDS. Microscopy with either calcofluor or modified trichrome stains is the standard diagnostic test for microsporidiosis and does not allow species identification. Detection of E. bieneusi infection based on PCR is limited to a few reference laboratories, and thus it is not the standard diagnostic assay. We have recently reported the development and characterization of a panel of monoclonal antibodies against E. bieneusi, and in this publication we evaluated the specificity and sensitivity of an immunofluorescence assay (IFA), compared with PCR, in simian immunodeficiency virus-infected macaques. The IFA, which correlated with the primary PCR method, with a detection limit of 1.5 x 10(5) spores per gram of feces, will simplify considerably the detection of E. bieneusi spores in clinical and environmental specimens and in laboratory and epidemiological investigations.</p>","PeriodicalId":72602,"journal":{"name":"Clinical and diagnostic laboratory immunology","volume":"12 10","pages":"1141-4"},"PeriodicalIF":0.0,"publicationDate":"2005-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/CDLI.12.10.1141-1144.2005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25625639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of serum antibody responses against the rotavirus nonstructural protein NSP4 in children after rhesus rotavirus tetravalent vaccination or natural infection.","authors":"Esmeralda Vizzi,&nbsp;Eva Calviño,&nbsp;Rosabel González,&nbsp;Irene Pérez-Schael,&nbsp;Max Ciarlet,&nbsp;Gagandeep Kang,&nbsp;Mary K Estes,&nbsp;Ferdinando Liprandi,&nbsp;Juan E Ludert","doi":"10.1128/CDLI.12.10.1157-1163.2005","DOIUrl":"https://doi.org/10.1128/CDLI.12.10.1157-1163.2005","url":null,"abstract":"<p><p>The immune response elicited by the rotavirus nonstructural protein NSP4 and its potential role in protection against rotavirus disease are not well understood. We investigated the serological response to NSP4 and its correlation with disease protection in sera from 110 children suffering acute diarrhea, associated or not with rotavirus, and from 26 children who were recipients of the rhesus rotavirus tetravalent (RRV-TV) vaccine. We used, as antigens in an enzyme-linked immunosorbent assay (ELISA), affinity-purified recombinant NSP4 (residues 85 to 175) from strains SA11, Wa, and RRV (genotypes A, B, and C, respectively) fused to glutathione S-transferase. Seroconversion to NSP4 was observed in 54% (42/78) of the children who suffered from natural rotavirus infection and in 8% (2/26) of the RRV-TV vaccine recipients. Our findings indicate that NSP4 evokes significantly (P < 0.05) higher seroconversion rates after natural infection than after RRV-TV vaccination. The serum antibody levels to NSP4 were modest (titers of < or = 200) in most of the infected and vaccinated children. A heterotypic NSP4 response was detected in 48% of the naturally rotavirus-infected children with a detectable response to NSP4. Following natural infection or RRV-TV vaccination, NSP4 was significantly less immunogenic than the VP6 protein when these responses were independently measured by ELISA. A significant (P < 0.05) proportion of children who did not develop diarrhea associated with rotavirus had antibodies to NSP4 in acute-phase serum, suggesting that serum antibodies against NSP4 might correlate with protection from rotavirus diarrhea. In addition, previous exposures to rotavirus did not affect the NSP4 seroconversion rate.</p>","PeriodicalId":72602,"journal":{"name":"Clinical and diagnostic laboratory immunology","volume":"12 10","pages":"1157-63"},"PeriodicalIF":0.0,"publicationDate":"2005-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/CDLI.12.10.1157-1163.2005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25626164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of immunoglobulin G subclass antibodies against recombinant Fasciola gigantica cathepsin L1 in an enzyme-linked immunosorbent assay for serodiagnosis of human fasciolosis.","authors":"Chaisiri Wongkham,&nbsp;Chairat Tantrawatpan,&nbsp;Pewpan M Intapan,&nbsp;Wanchai Maleewong,&nbsp;Sopit Wongkham,&nbsp;Kunio Nakashima","doi":"10.1128/CDLI.12.10.1152-1156.2005","DOIUrl":"https://doi.org/10.1128/CDLI.12.10.1152-1156.2005","url":null,"abstract":"<p><p>A cystatin capture enzyme-linked immunosorbent assay (ELISA) using recombinant Fasciola gigantica cathepsin L1 antigen was developed to detect specific immunoglobulin G (IgG) subclass antibodies (IgG1, IgG2, IgG3, and IgG4) and was evaluated for its diagnostic potential for human fasciolosis. In an analysis of the sera of 13 patients infected with F. gigantica, 209 patients with other parasitic infections, 32 cholangiocarcinoma patients, and 42 healthy controls, the IgG4-ELISA gave the highest diagnostic values. The sensitivity, specificity, accuracy, and positive and negative predictive values of this method based on the detection of IgG4 antibody were 100%, 99.3%, 99.3%, 86.7%, and 100%, respectively. The results revealed that restricting the ELISA to the detection of specific IgG4 antibody enhanced the specificity and accuracy for the serodiagnosis of human fasciolosis.</p>","PeriodicalId":72602,"journal":{"name":"Clinical and diagnostic laboratory immunology","volume":"12 10","pages":"1152-6"},"PeriodicalIF":0.0,"publicationDate":"2005-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/CDLI.12.10.1152-1156.2005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25626163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnosis of invasive pneumococcal disease among children in Kenya with enzyme-linked immunosorbent assay for immunoglobulin G antibodies to pneumococcal surface adhesin A.","authors":"J Anthony G Scott,&nbsp;Zena Mlacha,&nbsp;Joyce Nyiro,&nbsp;Salome Njenga,&nbsp;Pole Lewa,&nbsp;Jacktone Obiero,&nbsp;Hanningtone Otieno,&nbsp;Jacquelyn S Sampson,&nbsp;George M Carlone","doi":"10.1128/CDLI.12.10.1195-1201.2005","DOIUrl":"10.1128/CDLI.12.10.1195-1201.2005","url":null,"abstract":"<p><p>Diagnostic techniques for invasive pneumococcal disease (IPD) in children are insensitive and underestimate both the burden of disease and the cost-effectiveness of pneumococcal conjugate vaccination (PCV). Consequently, there is little demand for the highly effective PCV outside the United States and Europe. In Kenya, diagnosis of pneumococcal pneumonia in adults was achieved with a sensitivity of 0.70 and a specificity of 0.98 using enzyme-linked immunosorbent assays (ELISAs) of paired plasma samples for immunoglobulin G (IgG) to pneumococcal surface adhesin A (PsaA). We aimed to validate the same technique in children. We assayed paired blood samples from 98 children with IPD, 95 age-matched children with malaria/anemia, and 97 age-matched healthy controls by using an ELISA for anti-PsaA IgG. Sensitivity and specificity were determined in IPD patients and healthy controls. Specificity (0.97; 95% confidence interval [CI], 0.91 to 0.99) and sensitivity (0.42; 95% CI, 0.32 to 0.52) were optimized at a 2.7-fold rise in anti-PsaA antibody concentration. Sensitivity was improved to a maximum of 0.50 by restricting testing to children of <2 years old, by excluding IPD patients who were not sampled on the first day of presentation, and by incorporating high existing antibody concentrations in the analysis. Assay performance was independent of nasopharyngeal carriage of pneumococci at recruitment. This assay improves on existing diagnostic tools for IPD in children but would still leave over half of all cases undetected in epidemiological studies. Effective diagnosis of pneumococcal disease in children is urgently required but poorly served by existing technology.</p>","PeriodicalId":72602,"journal":{"name":"Clinical and diagnostic laboratory immunology","volume":"12 10","pages":"1195-201"},"PeriodicalIF":0.0,"publicationDate":"2005-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/CDLI.12.10.1195-1201.2005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25626170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feline coronavirus serotypes 1 and 2: seroprevalence and association with disease in Switzerland.","authors":"Maya Kummrow,&nbsp;Marina L Meli,&nbsp;Michael Haessig,&nbsp;Enikoe Goenczi,&nbsp;Amy Poland,&nbsp;Niels C Pedersen,&nbsp;Regina Hofmann-Lehmann,&nbsp;Hans Lutz","doi":"10.1128/CDLI.12.10.1209-1215.2005","DOIUrl":"https://doi.org/10.1128/CDLI.12.10.1209-1215.2005","url":null,"abstract":"<p><p>To determine the prevalence of antibodies to feline coronavirus (FCoV) serotypes 1 and 2 in Switzerland and their association with different disease manifestations, a serological study based on immunofluorescence tests was conducted with Swiss field cats using transmissible gastroenteritis virus (TGEV), FCoV type 1 and FCoV type 2 as antigens. A total of 639 serum samples collected in the context of different studies from naturally infected cats were tested. The current study revealed that, with an apparent prevalence of 83%, FCoV serotype 1 is the most prevalent serotype in Switzerland. FCoV type 1 viruses induced higher antibody titers than FCoV type 2, and were more frequently associated with clinical signs and/or feline infectious peritonitis. The antibody development in seven cats experimentally infected with FCoV type 1 revealed that, with progressing duration of infection, antibodies to FCoV type 1 significantly increased over those to FCoV type 2. There was a significant relationship between antibody titers against TGEV, FCoV 1, and FCoV 2 and TGEV antigen detected the highest proportion of seropositive cats. We conclude that a vaccine against FCoV should be based on FCoV type 1-related antigens and that for serodiagnosis of FCoV infection TGEV should be used to attain the highest diagnostic efficiency. When serology is used in addition to clinical signs, hematology, and clinical chemistry results as an aid to diagnose clinical FIP, TGEV shows a diagnostic efficiency equal to that of a FCoV antigen.</p>","PeriodicalId":72602,"journal":{"name":"Clinical and diagnostic laboratory immunology","volume":"12 10","pages":"1209-15"},"PeriodicalIF":0.0,"publicationDate":"2005-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/CDLI.12.10.1209-1215.2005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25624932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increased levels of macrophage migration inhibitory factor in sera of patients with Escherichia coli O157:H7-induced enterocolitis.","authors":"Tatsuya Ohkawara,&nbsp;Hiroshi Takeda,&nbsp;Masahiro Asaka,&nbsp;Yuka Mizue,&nbsp;Jun Nishihira","doi":"10.1128/CDLI.12.10.1257-1258.2005","DOIUrl":"https://doi.org/10.1128/CDLI.12.10.1257-1258.2005","url":null,"abstract":"Verotoxin-producing Escherichia coli O157:H7 is a pathogen that causes severe hemorrhagic enterocolitis and sometimes leads to serious conditions such as hemolytic-uremic syndrome. Various inflammatory parameters have been assessed for their efficacy in predicting the severity of E. coli O157:H7-induced colitis (9, 10), but no serological biomarker has been identified which can precisely determine the presence and severity of this colitis. Macrophage migration inhibitory factor (MIF) is a unique cytokine that has been shown to play a role in several inflammatory diseases (4, 7). We here report increased levels of MIF in sera of patients with verotoxin-producing E. coli O157:H7-induced enterocolitis. \u0000 \u0000Serum samples were obtained from 24 patients with E. coli O157:H7-induced enterocolitis, 30 sex- and age-matched patients with bacterial enterocolitis induced by pathogens other than O157:H7 (Salmonella, Vibrio, and non-verotoxigenic E. coli) as disease controls, and 55 healthy individuals as normal controls. Serum MIF concentrations were measured with an enzyme-linked immunosorbent assay specific for MIF (IDLISA; Sapporo Immunodiagnostic Laboratory, Sapporo, Japan) as described previously (7). E. coli O157:H7 was diagnosed by stool culture, which is the gold standard diagnostic method for this pathogen. \u0000 \u0000As shown in Fig. ​Fig.1,1, the serum MIF levels in patients with E. coli O157:H7-induced enterocolitis (23.67 ± 2.36 ng/ml; P < 0.001 versus normal controls and disease controls) were sixfold higher than those in normal controls and threefold higher than those in disease controls (3.99 ± 0.17 and 8.23 ± 0.60 ng/ml, respectively). Furthermore, serum MIF levels provided a level of differentiation of subjects with E. coli O157:H7-induced enterocolitis that was comparable to that afforded by detection of E. coli O157:H7 from stool culture (cutoff value, sensitivity, specificity, and positive predictive value by serum MIF: 12.5 ng/ml, 0.875, 0.950, and 0.840, respectively). On the other hand, leukocyte counts and levels of C-reactive protein in peripheral blood samples were increased in patients with E. coli O157:H7-induced enterocolitis and disease controls compared with normal controls, but there was no significant difference between patients with E. coli O157:H7-induced enterocolitis and disease controls (data not shown). \u0000 \u0000 \u0000 \u0000FIG. 1. \u0000 \u0000Serum MIF concentrations in infectious colitis induced by verotoxin-producing E. coli O157:H7. Values are means ± standard error. Serum MIF levels were measured in 55 normal controls (NC), 30 patients with bacterial colitis induced by pathogens ... \u0000 \u0000 \u0000 \u0000We previously demonstrated that MIF is a pivotal cytokine in the pathogenesis of inflammatory bowel disease and other types of colitis (8). In addition, recent studies have shown that MIF is immediately released in large quantities from the pituitary and other tissues in response to lipopolysaccharide (LPS) (1, 3). In particular, increased MIF levels in serum h","PeriodicalId":72602,"journal":{"name":"Clinical and diagnostic laboratory immunology","volume":"12 10","pages":"1257-8"},"PeriodicalIF":0.0,"publicationDate":"2005-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/CDLI.12.10.1257-1258.2005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25644716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lassa fever virus peptides predicted by computational analysis induce epitope-specific cytotoxic-T-lymphocyte responses in HLA-A2.1 transgenic mice.","authors":"Agnieszka Boesen, Krishnan Sundar, Richard Coico","doi":"10.1128/CDLI.12.10.1223-1230.2005","DOIUrl":"10.1128/CDLI.12.10.1223-1230.2005","url":null,"abstract":"<p><p>Lassa fever is a hemorrhagic disease caused by Lassa fever virus (LV). Although the precise host defense mechanism(s) that affords protection against LV is not completely understood, cellular immunity mediated by cytotoxic T lymphocytes (CTLs) plays a pivotal role in controlling viral replication and LV infection. To date, there have been no reports mapping major histocompatibility complex (MHC) class I-binding CTL epitopes for LV. Using computer-assisted algorithms, we identified five HLA-A2.1-binding peptides of LV glycoprotein (GP) and two peptides from LV nucleoprotein (NP). Synthesized peptides were examined for their ability to bind to MHC class I molecules using a flow cytometric assay that measures peptide stabilization of class I. Three of the LV-GP peptides tested (LLGTFTWTL, SLYKGVYEL, and YLISIFLHL) stabilized HLA-A2. The LV-NP peptides tested failed to stabilize this HLA-A2. We then investigated the ability of the HLA-A2-binding LV-GP peptides to generate peptide-specific CTLs in HLA-A2.1 transgenic mice. Functional assays used to confirm CTL activation included gamma interferon enzyme-linked immunospot (ELISPOT) assays and intracellular cytokine staining of CD8+ T cells from peptide-primed mice. CTL assays were also performed to verify the cytolytic activity of peptide-pulsed target cells. Each of the LV-GP peptides induced CTL responses in HLA-A2-transgenic mice. MHC class I tetramers prepared using one LV-GP peptide that showed the highest cytolytic index (LLGTFTWTL) confirmed that peptide-binding CD8+ T cells were present in pooled lymphocytes harvested from peptide-primed mice. These findings provide direct evidence for the existence of LV-derived GP epitopes that may be useful in the development of protective immunogens for this hemorrhagic virus.</p>","PeriodicalId":72602,"journal":{"name":"Clinical and diagnostic laboratory immunology","volume":"12 10","pages":"1223-30"},"PeriodicalIF":0.0,"publicationDate":"2005-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1247823/pdf/0101-05.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25624934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzyme-linked immunosorbent assays for detection of equine antibodies specific to Sarcocystis neurona surface antigens.","authors":"Jessica S Hoane,&nbsp;Jennifer K Morrow,&nbsp;William J Saville,&nbsp;J P Dubey,&nbsp;David E Granstrom,&nbsp;Daniel K Howe","doi":"10.1128/CDLI.12.9.1050-1056.2005","DOIUrl":"https://doi.org/10.1128/CDLI.12.9.1050-1056.2005","url":null,"abstract":"<p><p>Sarcocystis neurona is the primary causative agent of equine protozoal myeloencephalitis (EPM), a common neurologic disease of horses in the Americas. We have developed a set of enzyme-linked immunosorbent assays (ELISAs) based on the four major surface antigens of S. neurona (SnSAGs) to analyze the equine antibody response to S. neurona. The SnSAG ELISAs were optimized and standardized with a sample set of 36 equine sera that had been characterized by Western blotting against total S. neurona parasite antigen, the current gold standard for S. neurona serology. The recombinant SnSAG2 (rSnSAG2) ELISA showed the highest sensitivity and specificity at 95.5% and 92.9%, respectively. In contrast, only 68.2% sensitivity and 71.4% specificity were achieved with the rSnSAG1 ELISA, indicating that this antigen may not be a reliable serological marker for analyzing antibodies against S. neurona in horses. Importantly, the ELISA antigens did not show cross-reactivity with antisera to Sarcocystis fayeri or Neospora hughesi, two other equine parasites. The accuracy and reliability exhibited by the SnSAG ELISAs suggest that these assays will be valuable tools for examining the equine immune response against S. neurona infection, which may help in understanding the pathobiology of this accidental parasite-host interaction. Moreover, with modification and further investigation, the SnSAG ELISAs have potential for use as immunodiagnostic tests to aid in the identification of horses affected by EPM.</p>","PeriodicalId":72602,"journal":{"name":"Clinical and diagnostic laboratory immunology","volume":"12 9","pages":"1050-6"},"PeriodicalIF":0.0,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/CDLI.12.9.1050-1056.2005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25287999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endogenous superantigens shape response to exogenous superantigens.","authors":"Govindarajan Rajagopalan,&nbsp;Manisha Singh,&nbsp;Moon M Sen,&nbsp;Narayana S Murali,&nbsp;Karl A Nath,&nbsp;Chella S David","doi":"10.1128/CDLI.12.9.1119-1122.2005","DOIUrl":"https://doi.org/10.1128/CDLI.12.9.1119-1122.2005","url":null,"abstract":"<p><p>Endogenous superantigen-mediated thymic negative selection resulted in a paucity of mature T cells bearing T-cell receptor (TCR) Vbeta8 in the periphery. Consequently, the magnitude of immune response to exogenous superantigen staphylococcal enterotoxin B, which activates TCR Vbeta8(+) T cells, was significantly reduced and conferred protection from superantigen-induced mortality.</p>","PeriodicalId":72602,"journal":{"name":"Clinical and diagnostic laboratory immunology","volume":"12 9","pages":"1119-22"},"PeriodicalIF":0.0,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/CDLI.12.9.1119-1122.2005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25289850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}