Clinical and diagnostic laboratory immunology最新文献_第6页

Monoclonal antibodies against Enterocytozoon bieneusi of human origin. 抗人源双胞虫单克隆抗体。

Clinical and diagnostic laboratory immunology Pub Date : 2005-09-01 DOI: 10.1128/CDLI.12.9.1109-1113.2005

Abhineet S Sheoran, Xiaochuan Feng, Inderpal Singh, Susan Chapman-Bonofiglio, Sabrina Kitaka, Joel Hanawalt, John Nunnari, Keith Mansfield, James K Tumwine, Saul Tzipori

{"title":"Monoclonal antibodies against Enterocytozoon bieneusi of human origin.","authors":"Abhineet S Sheoran, Xiaochuan Feng, Inderpal Singh, Susan Chapman-Bonofiglio, Sabrina Kitaka, Joel Hanawalt, John Nunnari, Keith Mansfield, James K Tumwine, Saul Tzipori","doi":"10.1128/CDLI.12.9.1109-1113.2005","DOIUrl":"https://doi.org/10.1128/CDLI.12.9.1109-1113.2005","url":null,"abstract":"Enterocytozoon bieneusi is clinically the most significant among the microsporidia infecting humans, causing chronic diarrhea, wasting, and cholangitis in individuals with human immunodeficiency virus/AIDS. The lack of immune reagents is largely due to the absence of methods for laboratory propagation of E. bieneusi. We recently described a procedure for the concentration and purification of spores from diarrheic stool of infected humans. Purified spores were used to immunize mice for production and screening of monoclonal antibodies (MAbs) against E. bieneusi. The eight immunoglobulin M MAbs generated and fully characterized did not cross-react with other human microsporidia or with other microorganisms normally present in stool. One of the MAbs, 2G4, reacted with E. bieneusi spores in stools from monkeys and humans, without background fluorescence, which makes it an ideal diagnostic reagent. It also recognizes intracellular stages of the parasite and will be suitable for determining tissue distribution of E. bieneusi in infected hosts. At least two immunodominant antigens of E. bieneusi of 33,000 and 35,000 Da exist, which were recognized by rabbit and mouse antisera. The availability of MAbs against E. bieneusi will simplify considerably the diagnosis of this infection in humans and will provide tools for epidemiologic investigations regarding the true prevalence of the infection in various human and mammalian populations and the environmental sources of infection.","PeriodicalId":72602,"journal":{"name":"Clinical and diagnostic laboratory immunology","volume":"12 9","pages":"1109-13"},"PeriodicalIF":0.0,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/CDLI.12.9.1109-1113.2005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25289847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Comparison of the antibodies in lymphocyte supernatant and antibody-secreting cell assays for measuring intestinal mucosal immune response to a novel oral typhoid vaccine (M01ZH09). 新型口服伤寒疫苗(M01ZH09)肠黏膜免疫应答测定中淋巴细胞上清抗体与抗体分泌细胞抗体测定的比较

Clinical and diagnostic laboratory immunology Pub Date : 2005-09-01 DOI: 10.1128/CDLI.12.9.1127-1129.2005

B D Kirkpatrick, Matthew D Bentley, Anette M Thern, Catherine J Larsson, Cassandra Ventrone, Meera V Sreenivasan, Lou Bourgeois

引用次数: 25

Difference in cytokine production and cell activation between adenoidal lymphocytes and peripheral blood lymphocytes of children with otitis media. 中耳炎患儿腺样淋巴细胞与外周血淋巴细胞细胞因子产生及细胞活化的差异。

Clinical and diagnostic laboratory immunology Pub Date : 2005-09-01 DOI: 10.1128/CDLI.12.9.1130-1134.2005

Atsushi Harimaya, Jussi Tarkkanen, Petri Mattila, Nobuhiro Fujii, Jukka Ylikoski, Tetsuo Himi

引用次数: 4

Diagnosis of tetanus immunization status: multicenter assessment of a rapid biological test. 破伤风免疫状态的诊断:快速生物试验的多中心评估。

Clinical and diagnostic laboratory immunology Pub Date : 2005-09-01 DOI: 10.1128/CDLI.12.9.1057-1062.2005

Isabelle Colombet, Colette Saguez, Marie-José Sanson-Le Pors, Benoît Coudert, Gilles Chatellier, Pierre Espinoza

{"title":"Diagnosis of tetanus immunization status: multicenter assessment of a rapid biological test.","authors":"Isabelle Colombet, Colette Saguez, Marie-José Sanson-Le Pors, Benoît Coudert, Gilles Chatellier, Pierre Espinoza","doi":"10.1128/CDLI.12.9.1057-1062.2005","DOIUrl":"https://doi.org/10.1128/CDLI.12.9.1057-1062.2005","url":null,"abstract":"Diagnosis of tetanus immunization status by medical interview of patients with wounds is poor. Many protected patients receive unnecessary vaccine or immunoglobulin, and unprotected patients may receive nothing. The aim of this study is to evaluate the feasibility and accuracy of the Tetanos Quick Stick (TQS) rapid finger prick stick test in the emergency department for determining immunization status. We designed a prospective multicenter study for blinded comparison of TQS with an enzyme-linked immunosorbent assay (ELISA). Adults referred for open wounds in 37 French hospital emergency departments had the TQS after receiving standard care (emergency-TQS). TQS was also performed in the hospital laboratory on total blood (blood/lab-TQS) and serum (serum/lab-TQS). ELISA was performed with the same blood sample at a central laboratory. We assessed concordance between emergency-TQS and blood/lab-TQS by the kappa test and the diagnostic accuracy (likelihood ratios) of medical interview, emergency-TQS, and lab-TQS. ELISA was positive in 94.6% of the 988 patients included. Concordance between blood/emergency-TQS and blood/lab-TQS results was moderate (kappa=0.6), with a high proportion of inconclusive blood/emergency-TQS tests (9.8%). Likelihood ratios for immunization were 3.0 (95% confidence interval [CI], 1.8 to 5.1), 36.6 (95% CI, 5.3 to 255.3), 89.1 (95% CI, 5.6 to 1,405.0), and 92.7 (95% CI, 5.9 to 1,462.0) for medical interview, blood/emergency-TQS, blood/lab-TQS, and serum/lab-TQS, respectively. The sensitivity of the blood/emergency-TQS was 76.7%, and the specificity was 98% by reference to the ELISA. TQS use in the emergency room could make tetanus prevention more accurate if its technical feasibility were improved, and our assessment will be supplemented by a cost effectiveness study.","PeriodicalId":72602,"journal":{"name":"Clinical and diagnostic laboratory immunology","volume":"12 9","pages":"1057-62"},"PeriodicalIF":0.0,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/CDLI.12.9.1057-1062.2005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25288000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 40

Application of a stool antigen test to evaluate the incidence of Helicobacter pylori infection in children and adolescents from Tehran, Iran. 应用粪便抗原试验评估伊朗德黑兰儿童和青少年幽门螺杆菌感染的发生率。

Clinical and diagnostic laboratory immunology Pub Date : 2005-09-01 DOI: 10.1128/CDLI.12.9.1094-1097.2005

Tahereh Falsafi, Nargess Valizadeh, Shayesteh Sepehr, Mehri Najafi

{"title":"Application of a stool antigen test to evaluate the incidence of Helicobacter pylori infection in children and adolescents from Tehran, Iran.","authors":"Tahereh Falsafi, Nargess Valizadeh, Shayesteh Sepehr, Mehri Najafi","doi":"10.1128/CDLI.12.9.1094-1097.2005","DOIUrl":"https://doi.org/10.1128/CDLI.12.9.1094-1097.2005","url":null,"abstract":"Helicobacter pylori infection is acquired mainly in childhood, especially in developing countries, where a low-cost, rapid diagnostic technique which is reliable for all age groups may be useful for the management of H. pylori infection. For this purpose, we used an HpSA test (Equipar) to detect H. pylori infection in children and adolescents from Tehran, Iran. Thirty-five children who were positive or negative for H. pylori infection by endoscopy-based tests were used as positive and negative controls for the HpSA test. Stools were collected from 430 randomly selected children and adolescents (4 to 18 years old) from southwest, near the center, and northwest of Tehran. A questionnaire that included presence of recurrent abdominal pain (RAP), family history of infection and/or peptic ulcer disease (PUD), and income of parents was completed. A good agreement was found between the results of endoscopy-based tests and those of the HpSA test; the sensitivity and specificity of the Equipar-HpSA test were 100% and 83.4%, respectively. Among 430 children and adolescents, 47% were positive by the HpSA test, of whom 82% had RAP. No difference in incidence was observed between the two sexes; the various categories of age showed an increasing incidence, ranging from 24% (ages 4 to 6) to 58% (ages 16 to 18). The rate of infection in children and adolescents from the southwest was significantly higher (70%) than the rate in those from the northwest (32%), and a family history of H. pylori infection or PUD was observed in 59% of the HpSA positive subjects. The HpSA test is a useful test to detect H. pylori infection in children and adolescents from developing countries.","PeriodicalId":72602,"journal":{"name":"Clinical and diagnostic laboratory immunology","volume":"12 9","pages":"1094-7"},"PeriodicalIF":0.0,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/CDLI.12.9.1094-1097.2005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25288005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 48

The Women's Interagency HIV Study: an observational cohort brings clinical sciences to the bench. 妇女跨机构艾滋病毒研究:一项观察队列将临床科学带到板凳上。

Clinical and diagnostic laboratory immunology Pub Date : 2005-09-01 DOI: 10.1128/CDLI.12.9.1013-1019.2005

Melanie C Bacon, Viktor von Wyl, Christine Alden, Gerald Sharp, Esther Robison, Nancy Hessol, Stephen Gange, Yvonne Barranday, Susan Holman, Kathleen Weber, Mary A Young

引用次数: 517

Detection of immune complexes is not independent of detection of antibodies in Lyme disease patients and does not confirm active infection with Borrelia burgdorferi. 在莱姆病患者中，免疫复合物的检测并不独立于抗体的检测，也不能证实伯氏疏螺旋体的活动性感染。

Clinical and diagnostic laboratory immunology Pub Date : 2005-09-01 DOI: 10.1128/CDLI.12.9.1036-1040.2005

Adriana R Marques, Ronald L Hornung, Len Dally, Mario T Philipp

{"title":"Detection of immune complexes is not independent of detection of antibodies in Lyme disease patients and does not confirm active infection with Borrelia burgdorferi.","authors":"Adriana R Marques, Ronald L Hornung, Len Dally, Mario T Philipp","doi":"10.1128/CDLI.12.9.1036-1040.2005","DOIUrl":"https://doi.org/10.1128/CDLI.12.9.1036-1040.2005","url":null,"abstract":"The Borrelia burgdorferi-specific immune complex (IC) test, which uses polyethylene glycol (PEG) precipitation to isolate ICs from serum, has been used as a research test in the laboratory diagnosis of early Lyme disease (LD) and has been proposed as a marker of active infection. We examined whether B. burgdorferi-specific antibodies were present within PEG-precipitated ICs (PEG-ICs) in patients with LD, posttreatment Lyme disease syndrome, and controls, including individuals who received the outer surface protein A (OspA) vaccine. Using a B. burgdorferi whole-cell enzyme-linked immunosorbent assay (ELISA), we obtained positive PEG-IC results not only in patients with a history of LD, but also in individuals vaccinated with OspA vaccine. The frequency of positive PEG-IC ELISAs in OspA vaccinees was significantly higher with ELISA-reactive than with ELISA-negative unprocessed serum samples (P=0.001), demonstrating dependency between the tests. Similar results were found using samples from rhesus macaques infected with B. burgdorferi, uninfected macaques vaccinated with OspA, and controls. Therefore, testing for the presence of antibodies against B. burgdorferi in PEG-IC preparations is not more likely to reflect active infection than testing in unprocessed serum and should not be used in individuals who received the OspA vaccine.","PeriodicalId":72602,"journal":{"name":"Clinical and diagnostic laboratory immunology","volume":"12 9","pages":"1036-40"},"PeriodicalIF":0.0,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/CDLI.12.9.1036-1040.2005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25289308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Utilization of follow-up specimens from viremic blood donors to assess the value of west nile virus immunoglobulin G avidity as an indicator of recent infection. 利用病毒血症献血者的随访标本，评估西尼罗病毒免疫球蛋白G含量作为近期感染指标的价值。

Clinical and diagnostic laboratory immunology Pub Date : 2005-09-01 DOI: 10.1128/CDLI.12.9.1123-1126.2005

Harry E Prince, Mary Lapé-Nixon, Michael P Busch, Leslie H Tobler, Gregory A Foster, Susan L Stramer

引用次数: 9

Patients with pulmonary tuberculosis develop a strong humoral response against methylated heparin-binding hemagglutinin. 肺结核患者对甲基化肝素结合血凝素产生强烈的体液反应。

Clinical and diagnostic laboratory immunology Pub Date : 2005-09-01 DOI: 10.1128/CDLI.12.9.1135-1138.2005

Stefania Zanetti, Alessandra Bua, Giovanni Delogu, Cinzia Pusceddu, Maristella Mura, Franca Saba, Piero Pirina, Carlo Garzelli, Cono Vertuccio, Leonardo A Sechi, Giovanni Fadda

引用次数: 37

Detection of aerosolized Alternaria alternata conidia, hyphae, and fragments by using a novel double-immunostaining technique. 一种新的双免疫染色技术检测雾化的交替孢分生孢子、菌丝和片段。

Clinical and diagnostic laboratory immunology Pub Date : 2005-09-01 DOI: 10.1128/CDLI.12.9.1114-1116.2005

Brett James Green, Detlef Schmechel, Euan Roger Tovey

引用次数: 24