Chemistry methods : new approaches to solving problems in chemistry最新文献

Cover Picture: X-Ray Absorption Studies of Local Structure of Dilute Ionic Species in Molten Salts (Chem. Methods 7/2025) 封面图：熔盐中稀释离子种类局部结构的x射线吸收研究（化学）。方法7/2025)

IF 6.1

Chemistry methods : new approaches to solving problems in chemistry Pub Date : 2025-07-14 DOI: 10.1002/cmtd.70014

Kaifeng Zheng, Nirmalendu Patra, Nicholas Marcella, Alexander S. Ivanov, James F. Wishart, Alejandro R. Ballesteros, Ruchi Gakhar, Sheng Dai, Santanu Roy, Vyacheslav Bryantsev, Simerjeet K. Gill, Anatoly I. Frenkel

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Cover Feature: Synthetic Thermo-Sensitive Hydrogel Microspheres as Temperature Probes in an Optical Tweezers Setup (Chem. Methods 7/2025) 封面特征：合成热敏水凝胶微球作为光学镊子装置中的温度探头（化学）。方法7/2025)

IF 6.1

Chemistry methods : new approaches to solving problems in chemistry Pub Date : 2025-07-14 DOI: 10.1002/cmtd.70015

Shahar Goren, Nicole Edelstein-Pardo, Ran Glinowiecki, Amit Sitt, Raya Sorkin, Ayelet Lesman

{"title":"Cover Feature: Synthetic Thermo-Sensitive Hydrogel Microspheres as Temperature Probes in an Optical Tweezers Setup (Chem. Methods 7/2025)","authors":"Shahar Goren, Nicole Edelstein-Pardo, Ran Glinowiecki, Amit Sitt, Raya Sorkin, Ayelet Lesman","doi":"10.1002/cmtd.70015","DOIUrl":"https://doi.org/10.1002/cmtd.70015","url":null,"abstract":"The Cover Feature shows poly-N-isopropyl-acrylamide (PNIPAAm) microspheres on a glass slide in water. PNIPAAm is a temperature-sensitive polymer that undergoes a volume transition between 20 and 45 °C. The spheres were fluorescently labelled and imaged with confocal fluorescence microscopy at 22 °C (top left) and at 45 °C (bottom right). In their Research Article (DOI: 10.1002/cmtd.202400040), R. Sorkin, A. Lesman and co-workers explain how they used the volume transition of PNIPAAm microspheres to measure the temperature close to a powerful optical tweezers laser beam. Measuring the temperature directly by using PNIPAAm helps to conduct optical tweezers experiments, especially with biological samples, and provides an additional control variable.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture>\u0000 </div>\u0000 </figure>","PeriodicalId":72562,"journal":{"name":"Chemistry methods : new approaches to solving problems in chemistry","volume":"5 7","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cmtd.70015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144615183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Cover Picture: Deuterium NMR in Chiral Polymer-Based Bimesophasic Lyotropic Mixtures: From the Demixing Process to Analytical Applications (Chem. Methods 6/2025) 封面图片：手性聚合物双相溶性混合物中的氘核磁共振：从分离过程到分析应用（化学）。方法6/2025)

IF 6.1

Chemistry methods : new approaches to solving problems in chemistry Pub Date : 2025-06-04 DOI: 10.1002/cmtd.202580601

Thomas Julien, Boris Gouilleux, Bernard Rousseau, Michael Reggelin, Philippe Lesot

引用次数: 0

Synthetic Thermo-Sensitive Hydrogel Microspheres as Temperature Probes in an Optical Tweezers Setup 合成热敏水凝胶微球作为光镊装置中的温度探头

IF 6.1

Chemistry methods : new approaches to solving problems in chemistry Pub Date : 2025-05-29 DOI: 10.1002/cmtd.202400040

Shahar Goren, Nicole Edelstein-Pardo, Ran Glinowiecki, Amit Sitt, Raya Sorkin, Ayelet Lesman

{"title":"Synthetic Thermo-Sensitive Hydrogel Microspheres as Temperature Probes in an Optical Tweezers Setup","authors":"Shahar Goren, Nicole Edelstein-Pardo, Ran Glinowiecki, Amit Sitt, Raya Sorkin, Ayelet Lesman","doi":"10.1002/cmtd.202400040","DOIUrl":"https://doi.org/10.1002/cmtd.202400040","url":null,"abstract":"Optical tweezers have numerous applications in biochemistry and biophysics research. Many optical tweezers experiments require the use of high laser powers, having a substantial heating side effect that can influence experimental results. The degree of heating and its spatial profile, however, depends on experimental conditions and requires direct measurement. Various methods for local temperature measurement have been proposed, each with its advantages and drawbacks. Herein, the use of the temperature-sensitive poly(N-isopropyl-acrylamide) (PNIPAAm) microspheres to measure the local heating caused by optical tweezers is demonstrated and the temperature profile around the laser focus at varying laser powers is characterized. The main advantage of this method is its applicability to virtually any setup and experiment. Using simple image analysis, the temperature within the biologically relevant range can be determined with 1°–2° accuracy. Possible applications and limitations of this method are discussed.","PeriodicalId":72562,"journal":{"name":"Chemistry methods : new approaches to solving problems in chemistry","volume":"5 7","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cmtd.202400040","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144615488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Cover Picture: Continuous Titration Based Method for Rapid In-Solution Analysis of Non-Covalent Interactions (Chem. Methods 5/2025)

IF 6.1

Chemistry methods : new approaches to solving problems in chemistry Pub Date : 2025-05-13 DOI: 10.1002/cmtd.202580501

Philipp Willmer, Adam C. Hundahl, Rodolphe Marie, Henrik Jensen

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Deuterium NMR in Chiral Polymer-Based Bimesophasic Lyotropic Mixtures: From the Demixing Process to Analytical Applications 手性聚合物双相溶性混合物中的氘核磁共振：从脱混过程到分析应用

IF 6.1

Chemistry methods : new approaches to solving problems in chemistry Pub Date : 2025-05-08 DOI: 10.1002/cmtd.202500011

Thomas Julien, Boris Gouilleux, Bernard Rousseau, Michael Reggelin, Philippe Lesot

{"title":"Deuterium NMR in Chiral Polymer-Based Bimesophasic Lyotropic Mixtures: From the Demixing Process to Analytical Applications","authors":"Thomas Julien, Boris Gouilleux, Bernard Rousseau, Michael Reggelin, Philippe Lesot","doi":"10.1002/cmtd.202500011","DOIUrl":"https://doi.org/10.1002/cmtd.202500011","url":null,"abstract":"Among the order-sensitive (anisotropic) nuclear magnetic resonance (NMR) interactions, the residual 2H quadrupolar couplings measured at the level of natural abundance or on deuterated compounds dissolved in (chiral) oriented solvents, is a unique and relevant source of molecular information (isotopy, chirality, structure, configuration, etc.). Until now, the aligned media used as NMR solvents (lyotropic or not) have generally been monophasic, although isotropic-anisotropic biphasic systems have recently been exploited. As a promising possibility, herein, the potential of lyotropic bimesophasic samples is examined based on a mixture of immiscible helical polymers, which result in two demixed chiral nematic domains (stable over time), each with its own orientation properties. The extraction of two independent and nonlinearly related sets of anisotropic NMR data is achieved with spatially resolved 2H nD experiments. First applied to small achiral, prochiral and chiral deuterated compounds, the approach is extended to investigate nonenriched molecules by natural abundance deuterium 2D NMR. Here lyotropic bimesophasic chiral systems combining a polypeptide (PBLG) and a polyacetylene polymer (PLA or l-MSP) in organic solvents are examined in depth, in relation with several applications. The dynamic behavior of these systems (polymer demixing and exchange process) is studied by analyzing 2H EXSY, 2H relaxation and 2H DOSY data.","PeriodicalId":72562,"journal":{"name":"Chemistry methods : new approaches to solving problems in chemistry","volume":"5 6","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cmtd.202500011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144214121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Fast Multidimensional Flow Nuclear Magnetic Resonance at High Field for Real-Time Reaction Monitoring and Flow Synthesis 高场快速多维流核磁共振实时反应监测与流动合成

IF 6.1

Chemistry methods : new approaches to solving problems in chemistry Pub Date : 2025-05-05 DOI: 10.1002/cmtd.202400061

Margherita Bazzoni, Yuliia Horbenko, Nour El Sabbagh, Achille Marchand, Magdalena Grochowska-Tatarczak, Aurélie Bernard, Patrick Giraudeau, François-Xavier Felpin, Jean-Nicolas Dumez

{"title":"Fast Multidimensional Flow Nuclear Magnetic Resonance at High Field for Real-Time Reaction Monitoring and Flow Synthesis","authors":"Margherita Bazzoni, Yuliia Horbenko, Nour El Sabbagh, Achille Marchand, Magdalena Grochowska-Tatarczak, Aurélie Bernard, Patrick Giraudeau, François-Xavier Felpin, Jean-Nicolas Dumez","doi":"10.1002/cmtd.202400061","DOIUrl":"https://doi.org/10.1002/cmtd.202400061","url":null,"abstract":"Flow nuclear magnetic resonance (NMR) at high field is a powerful approach for the online monitoring of chemical reactions, which provides real-time quantitative and structural information. While 1D 1H spectra are commonly collected for NMR monitoring applications, their information content is limited because of peak overlap and assignment ambiguity. 2D NMR methods provide an opportunity to resolve peaks that overlap in 1D spectra and obtain correlation information that helps assignment. Their fast implementations additionally have durations (from sub-second to minute) that remain compatible with a large range of online reaction monitoring applications. They are also expected to benefit flow synthesis applications in which a flow reactor is hyphenated with a high-field NMR spectrometer. Herein, a selection of fast 2D NMR methods that are compatible with flow NMR applications is described: ultrafast 2D NMR and fast diffusion-ordered NMR spectroscopy. After a description of an experimental setup and current best practices, the principle behind each method and the way in which it can be implemented for continuously flowing samples are described. Also, the flow effects that are specific to these methods, for different settings, are described. These fast 2D NMR methods should be useful to users of flow NMR with an interest in reaction monitoring.","PeriodicalId":72562,"journal":{"name":"Chemistry methods : new approaches to solving problems in chemistry","volume":"5 6","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cmtd.202400061","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144214035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Hybrid Polarizing Solids with Extended Pore Diameters for Dissolution Dynamic Nuclear Polarization 扩展孔径杂化极化固体对溶解动态核极化的影响

IF 6.1

Chemistry methods : new approaches to solving problems in chemistry Pub Date : 2025-05-05 DOI: 10.1002/cmtd.202400068

Ekaterina V. Pokochueva, Nghia H. Le, Sylvie Guibert, Chloé Gioiosa, Quentin Stern, James Tolchard, Charlotte Bocquelet, Olivier Cala, Matthieu Cavaillès, Laurent Veyre, Otto Mankinen, Ville-Veikko Telkki, Chloé Thieuleux, Sami Jannin

{"title":"Hybrid Polarizing Solids with Extended Pore Diameters for Dissolution Dynamic Nuclear Polarization","authors":"Ekaterina V. Pokochueva, Nghia H. Le, Sylvie Guibert, Chloé Gioiosa, Quentin Stern, James Tolchard, Charlotte Bocquelet, Olivier Cala, Matthieu Cavaillès, Laurent Veyre, Otto Mankinen, Ville-Veikko Telkki, Chloé Thieuleux, Sami Jannin","doi":"10.1002/cmtd.202400068","DOIUrl":"https://doi.org/10.1002/cmtd.202400068","url":null,"abstract":"Dissolution dynamic nuclear polarization (dDNP) has emerged as a powerful technique to address the inherent sensitivity limitations in nuclear magnetic resonance imaging and spectroscopy. The technique relies on the use of stable paramagnetic polarizing agents that act as a source of hyperpolarization but unfortunately, their presence contaminates the sample and significantly enhances paramagnetic relaxation of the hyperpolarized solutions. In the last decade, novel sample formulations have been proposed to combat this issue, including silica-based hybrid polarizing solids (HYPSO), which have enabled the production of contamination-free hyperpolarization. Herein, a new generation of polarizing solids with pore diameters increased from 4 up to 28 nm is presented. Compared to the previously described HYPSOs with smaller pore sizes of 4 nm, larger pores ultimately achieve the ambitious contradictory goals of 1) providing rapid and high solid-state polarization and improving cross-polarization efficiency, while 2) decreasing hyperpolarization losses upon melting, transfer, and filtration, and 3) reducing the overall concentration of the polarizing agent by a factor of 2. These HYPSO materials can be used in virtually any existing dDNP settings without modification, apart from the sole addition of an in-line commercial filter to retain the HYPSO powder upon dissolution and transfer.","PeriodicalId":72562,"journal":{"name":"Chemistry methods : new approaches to solving problems in chemistry","volume":"5 6","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cmtd.202400068","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144214036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Multiplex Cellular Microenvironment Detection Based on Surface-Enhanced Raman Scattering Patch 基于表面增强拉曼散射贴片的多路细胞微环境检测

IF 6.1

Chemistry methods : new approaches to solving problems in chemistry Pub Date : 2025-05-02 DOI: 10.1002/cmtd.202400083

Xiaoyu Wu, Zhaodong Zhang, Feng Zhu, Yuanheng Kuang, Shuqi Chen, Chongling Sun, Bohua Liu, Yanyan Wang

{"title":"Multiplex Cellular Microenvironment Detection Based on Surface-Enhanced Raman Scattering Patch","authors":"Xiaoyu Wu, Zhaodong Zhang, Feng Zhu, Yuanheng Kuang, Shuqi Chen, Chongling Sun, Bohua Liu, Yanyan Wang","doi":"10.1002/cmtd.202400083","DOIUrl":"https://doi.org/10.1002/cmtd.202400083","url":null,"abstract":"Monitoring cellular microenvironment is of vital significance to study cell process, reveal pathological state, and predict early disease. Herein, a surface-enhanced Raman scattering (SERS) patch is proposed to sense the hydrogen peroxide (H2O2) concentration and pH variation of extracellular microenvironment in a multiplex manner. Scale-up and ordered gold nanorods (AuNRs) assembly is introduced by interfacial assembly and absorption transferring on the flexible polydimethylsiloxane (PDMS) film, providing the patch with great SERS enhancement for biosensing. The modification of H2O2-responsive and pH-susceptive Raman reporter molecule endows the SERS patch with high sensitivity to multiple analytes, and the construction of multivariate curve resolution (MCR) analysis facilitates the quantification of H2O2 and pH value in spite of the characteristic peaks overlap. Furthermore, the SERS patch is used to compare the extracellular microenvironments of different living cells. Results demonstrate that highly migratory cells (i.e., MDA-MB-231) secreted more H2O2 under phorbol 12-myristate 13-acetate (PMA) stimulation, and the extracellular microenvironment is more acidic than the cells with weak migration ability (i.e., MCF-7), revealing the relationship between cell migration and microenvironment. The strategy integrates H2O2 detection and pH determination in one patch, which may offer an approach for novel multiplex biosensor and cell migration investigation.","PeriodicalId":72562,"journal":{"name":"Chemistry methods : new approaches to solving problems in chemistry","volume":"5 7","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cmtd.202400083","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144615289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Rapid, In Situ and Non-Destructive Analysis for the Evaluation of Microplastics Degradation in Water Via Haze Measurement 基于雾霾测量的水中微塑料降解的快速、原位和无损分析

IF 6.1

Chemistry methods : new approaches to solving problems in chemistry Pub Date : 2025-04-28 DOI: 10.1002/cmtd.202400047

Rossella Greco, Lucía Baxauli-Marin, Javier Fernández-Catalá, Viktorija Pankratova, Claudiu Bulbucan, Karina Thånell, Wei Cao

{"title":"Rapid, In Situ and Non-Destructive Analysis for the Evaluation of Microplastics Degradation in Water Via Haze Measurement","authors":"Rossella Greco, Lucía Baxauli-Marin, Javier Fernández-Catalá, Viktorija Pankratova, Claudiu Bulbucan, Karina Thånell, Wei Cao","doi":"10.1002/cmtd.202400047","DOIUrl":"https://doi.org/10.1002/cmtd.202400047","url":null,"abstract":"The presence of microplastics (MPs) in water represents a current problem for marine life and a future serious issue for human health. This is pushing towards new approaches for the removal of MPs and, hence, their characterization and evaluation, which up to now has represented a limit for tentative procedures for their degradation. Herein, we reported on a new facile, in situ and non-destructive approach for the qualitative analysis of polystyrene and polymethylmethacrylate MPs in water and their evaluation in photocatalytic degradation with the use of Co3O4@Co(OH)2 heterostructures. The use of haze measurements allows to see the changes in light scattering of the MPs aqueous dispersions during the photocatalytic degradation and to know in situ the degradation of the MPs in the catalytic process. Significant changes in haze values were found in photocatalysis with Co3O4@Co(OH)2, in contrast to no variations in control test using Co(OH)2 and Co3O4. Synchrotron-radiation based scanning transmission x-ray spectroscopy further denotes strong interactions between the heterostructures with MPs and oxidization of the latter. The possible comparison of different techniques suggests that haze measurements could be a valid and cost-efficient methodology for the future evaluation of photocatalytic degradation of MPs in water.","PeriodicalId":72562,"journal":{"name":"Chemistry methods : new approaches to solving problems in chemistry","volume":"5 6","pages":""},"PeriodicalIF":6.1,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cmtd.202400047","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144214255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0