Advances in Environmental Biology最新文献_第3页

Antimicrobial Activities and Molecular Signature of Endophytic Fungi of Wild Plant, Saudi Arabia 沙特阿拉伯野生植物内生真菌的抑菌活性和分子特征

Advances in Environmental Biology Pub Date : 2022-02-01 DOI: 10.22587/aeb.2022.16.2.1

Asma Alsini, W. Alshehri, R. Ashy, R. Gashgari

{"title":"Antimicrobial Activities and Molecular Signature of Endophytic Fungi of Wild Plant, Saudi Arabia","authors":"Asma Alsini, W. Alshehri, R. Ashy, R. Gashgari","doi":"10.22587/aeb.2022.16.2.1","DOIUrl":"https://doi.org/10.22587/aeb.2022.16.2.1","url":null,"abstract":"Endophytic fungi, which have been reported in numerous plant species, are essential components of the wild plants’ community and contribute significantly to natural ecosystems' diversity. The study aims to evaluate and characterize, at the molecular level, the diversity and antimicrobial activities of endophytic fungi from wild plants in Taif, Saudi Arabia. Fungal endophytes were isolated from leaves and stems of plants, including Juniperus excelsa, Ziziphus spina-christi, Aacacia gerradii, Rumex nervosus, Lavandula dentate, Hypoestes forskaolii, Ficus palmata, Eucalyptus smithii and Conyza bonariensis collected from Taif, Saudi Arabia. About 25 different species belonging to 16 genera were recovered. The ribosomal DNA internal transcribed spacer (ITS) regions of the collected endophytic isolates were sequenced. The most prevalent fungi were identified as Hypocopra, Aspergillus, Coniothyrium, Chaetomium, Bipolaris, Dothideomycetes, Penicillium, Phoma, Mucor, Readeriella, Leptosphaerulina, Neodidymelliopsis, and Thielavia. Seven of the endophytic fungi isolates exhibited vigorous antimicrobial activity to some microbial pathogens tested. Fungal sp. has the highest inhibition against the human pathogenic bacteria Escherichia coli, Candida albicans, Staphylococcus aureus, Enterococeus faecium and Streptococcus agalactiae","PeriodicalId":7250,"journal":{"name":"Advances in Environmental Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88058836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Detection of SHIGA-TOXIN producing E. coli in some retail markets in Egypt using qPCR assay with special reference to serotyping 使用qPCR法检测埃及部分零售市场中产生志贺毒素的大肠杆菌，并特别参考血清分型

Advances in Environmental Biology Pub Date : 2022-01-01 DOI: 10.22587/aeb.2022.16.1.1

M. Abdelmonem, Mohamed A. Kelany, M. Fawzy, R. Sheta, A. Ageez, Sherein I. Abd El‐Moez

{"title":"Detection of SHIGA-TOXIN producing E. coli in some retail markets in Egypt using qPCR assay with special reference to serotyping","authors":"M. Abdelmonem, Mohamed A. Kelany, M. Fawzy, R. Sheta, A. Ageez, Sherein I. Abd El‐Moez","doi":"10.22587/aeb.2022.16.1.1","DOIUrl":"https://doi.org/10.22587/aeb.2022.16.1.1","url":null,"abstract":"Background: Shiga toxin-producing Escherichia coli are dangerous foodborne pathogens that represent a severe public health issue worldwide. Raw foods are considered an important source of STEC infection in humans. Objective: In the current study, STEC contamination was investigated in 80 raw foods (chicken, beef, and milk) and water collected from different localities of Greater Cairo using Real-Time PCR. Moreover, the virulence genes of isolates were characterized. Results: STEC was detected in eight samples, (7 beef and 1 chicken), which represent about 10% of the tested samples. Positive beef and chicken samples show the presence of 13 and 2 STEC genes, respectively. Five samples were positive for eaeA (intemin), two samples were positive for stx1 gene and eight samples were positive for stx2 gene. Beef samples reveal the highest incidence of virulence gene stx2 (35%), followed by eaeA (20%), then stx1 (10%). The incidence of STEC was lower in chicken samples and the prevalence of virulence gene was 5% for stx2 and eaeA, respectively. Stx2 gene was the most prevalent subtype identified in beef samples. Serotyping of isolated STEC strains (14) revealed isolation of seven STEC strains belong to O157 serogroup, two strains belong to O111and five strains belong to serogroup O26. Conclusion: The current study concluded that recovery of STEC from raw chicken and beef samples is of important concern.","PeriodicalId":7250,"journal":{"name":"Advances in Environmental Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88062496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening Different Desert Soils in Makkah Province to Detect Novel Antibiotics Producing Actinomycetes 筛选麦加省不同沙漠土壤检测新型抗生素产放线菌

Advances in Environmental Biology Pub Date : 2021-12-01 DOI: 10.22587/aeb.2021.15.12.2.

Abdullah AI Hausawi, N. Baeshen, A. Al-Hejin, Mohammed A. AlMatary, Saleh M. Almaaqar, Mohamed Morsi Mohamed Ahmed

{"title":"Screening Different Desert Soils in Makkah Province to Detect Novel Antibiotics Producing Actinomycetes","authors":"Abdullah AI Hausawi, N. Baeshen, A. Al-Hejin, Mohammed A. AlMatary, Saleh M. Almaaqar, Mohamed Morsi Mohamed Ahmed","doi":"10.22587/aeb.2021.15.12.2.","DOIUrl":"https://doi.org/10.22587/aeb.2021.15.12.2.","url":null,"abstract":"The purpose of this study was to isolate, purify, and characterize actinomycetes from desert soil surrounding some plants scattered in Makkah Region using biochemical, morphological, and 16S rRNA gene sequencing techniques, as well as to conduct a taxonomic study of species that show activity against some pathogens and to study the characteristics and properties of antibiotics extracted. Soil samples were collected from three locations in Saudi Arabia's Makkah region. They were brought to the lab and cultured for four days at 27° C using actinomistate isolation media. We obtained eight samples based on physiological, morphological, and 16S rRNA gene sequences: Streptomyces spiralis, Streptomyces spinoverrucosus, Streptomyces fimbriatus, Streptomyces carpinensis, Streptomyces geysiriensis, Streptomyces djakartensis, Streptomyces levis, and Streptomyces atacamensis. All isolates of Streptomyces demonstrated antibacterial efficacy against Streptococcus pneumonia, Methicillin-resistant Staphylococcus aureus, Staphylococcus aureus, Streptococcus pyogenes, and antifungal including Aspergillus niger and Candida albicans. These isolates had antimicrobial efficacy and might be used to create novel antibiotics for medicinal or agricultural application.","PeriodicalId":7250,"journal":{"name":"Advances in Environmental Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82998598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tissue culture technique on micropropagation of grape B41 葡萄B41的组织培养技术

Advances in Environmental Biology Pub Date : 2021-12-01 DOI: 10.22587/aeb.2021.15.1.12

M. Al-Sameen, Khalil Al-Ma’ari, Fahd Al-Bisky

{"title":"Tissue culture technique on micropropagation of grape B41","authors":"M. Al-Sameen, Khalil Al-Ma’ari, Fahd Al-Bisky","doi":"10.22587/aeb.2021.15.1.12","DOIUrl":"https://doi.org/10.22587/aeb.2021.15.1.12","url":null,"abstract":"This research was carried out in the Tissue Culture Lab of the National Commission of Biotechnology (NCBT) in Damascus/ Syria and in Faculty of Agriculture– Damascus University during 2018- 2019 to optimize an effective method for micropropagation of grape Vitis vinifera L. Explants of (0.5-1cm) with adjacent bud were planted in multiplying stage on MS medium contains different concentrations of BAP (0, 0.5, 1, 1.5, 2, 3 mg/ l ), with or without 0.2 mg/ l NAA or IAA. The multiplied explants transferred in comparison with MS medium without hormones (control) with different concentrations of IAA (1, 2, 4, 8 mg/ l) for its rooting stage. The results revealed that the hormone combinations (2 mg/ l BAP + 0.2 mg/ l NAA) had the highest average of the plant length (3.233 cm), leaves and the number of formed buds (5.667 leaves, 4.667 bud/ plant biopsy respectively). The hormonal combinations BAP/ NAA led to an obvious increase in the studied parameters in comparison to hormonal combinations treatments BAP/ IAA and nutrition medium with BAP only treatments, whereas the hormonal combinations IAA/ NAA did not lead to an obvious increase in the number of leaves and formed buds in compare with nutrition medium with BAP only treatments. The best percentage of rooting (92.8%) was achieved in MS medium supplemented with (1 mg/ l IAA). 87% of the adapted produced plantlets from tissue culture were successfully moved and planted in soil.","PeriodicalId":7250,"journal":{"name":"Advances in Environmental Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83077663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial & Molecular Identification of Microbiota of Processed Chicken Products 鸡肉加工产品微生物与微生物分子鉴定

Advances in Environmental Biology Pub Date : 2021-12-01 DOI: 10.22587/aeb.2021.15.12.3.

Ahmed Talat Mohamed Ali Nemer, M. Mutwakil, M. Al-Matary

{"title":"Microbial & Molecular Identification of Microbiota of Processed Chicken Products","authors":"Ahmed Talat Mohamed Ali Nemer, M. Mutwakil, M. Al-Matary","doi":"10.22587/aeb.2021.15.12.3.","DOIUrl":"https://doi.org/10.22587/aeb.2021.15.12.3.","url":null,"abstract":"Poultry meat and meat products are the major sources of animal protein in the human diet throughout the world; however, they can be contaminated by various microorganisms. In this study, we isolated bacterial strains from chicken nuggets, chicken burgers, chicken franks, chicken balls, chicken kebab, chicken mince (frozen), and chicken mortadella purchased from two different retail stores located in Makkah. Bacterial strains were then characterized based on their biochemical features and 16S rRNA gene phylogeny. The isolates were also examined for their susceptibility to antibiotics. In this study, a total of fifty-one strains were isolated. Among them, forty-two strains were gram-positive and only nine strains were gram-negative bacteria. Thirty-two strains were oxidase-positive, and forty-two isolates were catalase-positive. 16S rRNA gene phylogeny showed that the bacteria belonged to sixteen genera, including Neisseria, Brevibacillus, Bacillus, Exiguobacterium, Rothia, Staphylococcus, Lysinibacillus, Kocuria, Sphingomonas, Novosphingobium, Enterococcus, Pelomonas, Paenibacillus, Klebsiella, Morganella, and Lactococcus. Bacterial strains responded differently against different antibiotics. The isolates A12 and A34 showed resistance to the highest number of antibiotics tested. Overall, the present study revealed that various poultry meat products in the studied area were contaminated by bacterial populations, and therefore, it is essential to investigate stores regularly to reduce microbial contamination in order to ensure public health.","PeriodicalId":7250,"journal":{"name":"Advances in Environmental Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72704007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Cryopreservation of grapevine shoot tips by frozen in vitro 葡萄藤茎尖的体外冷冻保存

Advances in Environmental Biology Pub Date : 2021-11-01 DOI: 10.22587/aeb.2021.15.11.1

M. Al-Sameen, Khalil Al-Ma’ari, Fahd Al-Bisky

{"title":"Cryopreservation of grapevine shoot tips by frozen in vitro","authors":"M. Al-Sameen, Khalil Al-Ma’ari, Fahd Al-Bisky","doi":"10.22587/aeb.2021.15.11.1","DOIUrl":"https://doi.org/10.22587/aeb.2021.15.11.1","url":null,"abstract":"This research was carried out in the Tissue Culture Lab of the National Commission of Biotechnology (NCBT) in Damascus/ Syria and in Faculty of Agriculture– Damascus University during 2018- 2019 to optimize an effective method for micropropagation of grape Vitis vinifera L. Explants of (0.5-1cm) with adjacent bud were planted in multiplying stage on MS medium contains different concentrations of BAP (0, 0.5, 1, 1.5, 2, 3 mg/ l ), with or without 0.2 mg/ l NAA or IAA. The multiplied explants transferred in comparison with MS medium without hormones (control) with different concentrations of IAA (1, 2, 4, 8 mg/ l) for its rooting stage. The results revealed that the hormone combinations (2 mg/ l BAP + 0.2 mg/ l NAA) had the highest average of the plant length (3.233 cm), leaves and the number of formed buds (5.667 leaves, 4.667 bud/ plant biopsy respectively). The hormonal combinations BAP/ NAA led to an obvious increase in the studied parameters in comparison to hormonal combinations treatments BAP/ IAA and nutrition medium with BAP only treatments, whereas the hormonal combinations IAA/ NAA did not lead to an obvious increase in the number of leaves and formed buds in compare with nutrition medium with BAP only treatments. The best percentage of rooting (92.8%) was achieved in MS medium supplemented with (1 mg/ l IAA). 87% of the adapted produced plantlets from tissue culture were successfully moved and planted in soil.","PeriodicalId":7250,"journal":{"name":"Advances in Environmental Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77064177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Fermentation Time on The Dynamics of Lactobacillus plantarum Bacteria using Collagen Extract from Broiler Claw Skin as a Substrate 以肉仔鸡爪皮胶原提取物为底物发酵时间对植物乳杆菌动态的影响

Advances in Environmental Biology Pub Date : 2021-10-01 DOI: 10.22587/aeb.2021.15.10.1

Muhammad Irfan Said, E. Abustam, Hasma A, Muhammad Fatahillah Arifin, S. Sirajuddin, A. R. Al-Tawaha, A. Al-Tawaha

引用次数: 0

Feeding and bioremediation relationships between Chrysochromulina species and bacteria 嗜黄菌与细菌的取食及生物修复关系

Advances in Environmental Biology Pub Date : 2021-09-01 DOI: 10.22587/aeb.2021.15.9.1

Maryam Sultan Al-Nahdi, Fotoon Sayegh

引用次数: 1

Farmers' Ability to Supply Corn to Village Collectors in Takalar Regency, South Sulawesi Province, Indonesia 印度尼西亚南苏拉威西省Takalar县农民向乡村收集者供应玉米的能力

Advances in Environmental Biology Pub Date : 2021-07-01 DOI: 10.22587/aeb.2021.15.7.1

I. Rasyid, S. Sirajuddin, Hastang H, Nirwana N

引用次数: 0

http://www.aensiweb.net/AENSIWEB/aeb/aeb_June_2021.html http://www.aensiweb.net/AENSIWEB/aeb/aeb_June_2021.html

Advances in Environmental Biology Pub Date : 2021-06-01 DOI: 10.22587/aeb.2021.15.6.1

Faraj Abdullah Al-Ghamd, Abdulrahaman Saeed Hajer, Hassan Saeed Al Zahran, Rashed Musaed Al Sabehi

引用次数: 0