Dóra Lili Brassó, Renáta Knop, Zsófia Várszegi, Péter Bársony, István Komlósi, Árpád Bacsadi, Andrea Bistyák
{"title":"Assessment of the microbiological status of two Hungarian ostrich farms.","authors":"Dóra Lili Brassó, Renáta Knop, Zsófia Várszegi, Péter Bársony, István Komlósi, Árpád Bacsadi, Andrea Bistyák","doi":"10.1556/004.2023.00765","DOIUrl":"https://doi.org/10.1556/004.2023.00765","url":null,"abstract":"<p><p>The aim of this study was to estimate the occurrence of bacterial infection and contamination in two ostrich-producing farms. Compared to other poultry species, the hatchability of ostrich eggs is especially low. In a quest to identify factors that may affect hatchability, we collected faecal samples from adult birds, as well as eggs with dead-in-shell embryos, dead chicks and swab samples from the surface of the eggs and from the environment. The samples were screened for the presence of bacteria by routine bacteriological culture methods. The most prevalent bacteria, detected in the samples, were Escherichia coli, Bacillus spp. and coliform bacteria, whereas Pseudomonas spp. were less frequently found. The intensity and species compositon of the bacterial contamination was comparable in the two farms. Our results revealed that the bacteria, present in the environment, may likely be transmitted to the surface of the eggs. If they are able to penetrate the shell then the embryos and chicks become infected easily. These findings draw the attention to the special importance of enforcing efficient decontamination and disinfection measures to keep the environment and egg surface free from germs. Besides the appropriate egg treatment procedure, the incubation and hatching technology should also be kept under control.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":"71 1","pages":"3-11"},"PeriodicalIF":0.9,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9697334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Autologous platelet-rich plasma treatment in a dog with vincristine extravasation injury.","authors":"Basak Boztok Ozgermen","doi":"10.1556/004.2023.00730","DOIUrl":"https://doi.org/10.1556/004.2023.00730","url":null,"abstract":"<p><p>Extravasation of vincristine sulfate (VS) is a common complication encountered during canine transmissible venereal tumor treatment and other hematopoietic neoplasms, such as lymphoma and leukemias. The leakage of the chemotherapeutic agent may result in soft tissue damage leading to ulceration and necrosis. Studies have shown that platelet-rich plasma consists of growth factors and cytokines, thus plays a crucial role in wound healing. This study aimed to evaluate the effects of autologous platelet-rich plasma and antimicrobial wound dressing for the treatment of VS extravasation-related acute full-thickness skin wounds. A male Aksaray Malakli shepherd dog (3 years old) was brought to our clinic with a wound on the left forelimb which appeared two days after intravenous injection of VS. Extravasation was suspected. A total of 3.5 mL autologous platelet-rich plasma was injected into the wound margins and an antimicrobial dressing containing chlorhexidine acetate was used weekly to close the wound. The wound healed without any complications in 6 weeks. It was concluded that autologous platelet-rich plasma treatment is a simple and cost-effective method for the treatment of VS extravasation-related acute full-thickness skin wounds.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":"71 1","pages":"25-29"},"PeriodicalIF":0.9,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9751183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Katarzyna Paździor-Czapula, Joanna Fiedorowicz, Iwona Otrocka-Domagała
{"title":"Myofibroblastic tumour in the liver of a cat - A case report.","authors":"Katarzyna Paździor-Czapula, Joanna Fiedorowicz, Iwona Otrocka-Domagała","doi":"10.1556/004.2023.00840","DOIUrl":"https://doi.org/10.1556/004.2023.00840","url":null,"abstract":"<p><p>This study presents a case of a primary hepatic myofibroblastic tumour in a 15-year-old European Shorthair female cat. The cat showed a gradual increase in liver enzymes (alanine aminotransferase and aspartate aminotransferase), and an abdominal ultrasound revealed a tumour located within the left lateral lobe of the liver. The tumour was surgically excised and sent for histopathology. Histopathological examination showed that the tumour was composed of homogeneous fusiform cells with low mitotic count, crowded within the perisinusoidal, portal and interlobular spaces, and entrapment of hepatocytes and bile ducts. Immunohistochemistry revealed that the tumour cells expressed vimentin and α-SMA, and were negative to desmin and cytokeratins. Based on the histological and immunohistochemical features, as well as some similarities with analogous entities in humans and animals, the tumour was classified as a myofibroblastic neoplasm originating from the liver.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":"71 1","pages":"30-33"},"PeriodicalIF":0.9,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9697341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zeynep Karapınar, Fatma İlhan, Mustafa Usta, Mehmet Özkan Timurkan
{"title":"Pathological and molecular investigation of canine distemper virus: Phylogenetic analysis of co-circulating genetic lineages in Türkiye.","authors":"Zeynep Karapınar, Fatma İlhan, Mustafa Usta, Mehmet Özkan Timurkan","doi":"10.1556/004.2023.00809","DOIUrl":"https://doi.org/10.1556/004.2023.00809","url":null,"abstract":"<p><p>Canine distemper virus (CDV) is a highly contagious virus that infects a wide variety of animals of carnivore species and may cause manifestations from subclinical infection to fatal disease. In this study, dogs clinically suspected having distemper were examined by reverse transcriptase-polymerase chain reaction (RT-PCR), histopathology and immuno-histochemistry. By histopathological examination, characteristic intracytoplasmic and/or intranuclear inclusion bodies were observed in the lung, stomach, small intestine, liver, kidney, spleen and central nervous system. Interstitial and broncho-interstitial pneumonia, gastroenteritis and encephalitis were revealed. CDV antigens were detected in all tissues with characteristic histopathological findings. The antigens were more abundant in the bronchial and bronchiolar epithelium and in the syntitial cells. Phylogenetic analyses were performed using the PCR-amplified partial sequences of the genes encoding the viral heamagglutinin and fusion proteins. The phylogenetic trees showed that the newly determined sequences were diverse and clustered within different lineages of the European or the Arctic strains.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":"71 1","pages":"54-64"},"PeriodicalIF":0.9,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9698579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maria Helena Barros Pinto, Thiago Rodrigues Cardoso Braga, Guilherme Reis Blume, Letícia Batelli de Oliveira, Nicolas Thomas Costa das Chagas, Fernanda Rezende Souza, Geovanni Dantas Cassali, Fabiano José Ferreira de Sant'Ana
{"title":"Metastatic exocrine pancreatic adenocarcinoma in a giant otter (Pteronura brasiliensis).","authors":"Maria Helena Barros Pinto, Thiago Rodrigues Cardoso Braga, Guilherme Reis Blume, Letícia Batelli de Oliveira, Nicolas Thomas Costa das Chagas, Fernanda Rezende Souza, Geovanni Dantas Cassali, Fabiano José Ferreira de Sant'Ana","doi":"10.1556/004.2023.00888","DOIUrl":"10.1556/004.2023.00888","url":null,"abstract":"<p><p>Neoplasms of the exocrine pancreas are uncommon in domestic animals and rarely occur in wildlife. This article describes the clinical and pathological findings of one case of metastatic exocrine pancreatic adenocarcinoma in an 18-year-old giant otter (Pteronura brasiliensis) in captivity with a history of inappetence and apathy. Abdominal ultrasonography was inconclusive, and tomography revealed a neoplasm affecting the urinary bladder and hydroureter. During the anaesthesia recovery, the animal presented a cardiorespiratory arrest and died. Grossly, there were neoplastic nodules in the pancreas, urinary bladder, spleen, adrenal glands, and mediastinal lymph node. Microscopically, all nodules were composed of a malignant hypercellular proliferation of epithelial cells with acinar or solid disposition, supported by a sparse fibrovascular stroma. Neoplastic cells were immunolabeled with antibodies to Pan-CK, CK7, CK20, PPP and chromogranin A. Approximately 25% of the cells were positive for the presence of Ki-67 too. Pathological and immunohistochemical findings confirmed the diagnosis of metastatic exocrine pancreatic adenocarcinoma.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":"71 1","pages":"41-45"},"PeriodicalIF":0.9,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9751174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Károly Vörös, Zsolt Becker, Zoltán Dudás Györki, Bernd Schulze Gronover, Ferenc Szalay
{"title":"Ultrasonography of the paralumbar muscles as a new aid during melarsomine treatment in canine heartworm disease. Description and illustration of the method - A preliminary study.","authors":"Károly Vörös, Zsolt Becker, Zoltán Dudás Györki, Bernd Schulze Gronover, Ferenc Szalay","doi":"10.1556/004.2022.00034","DOIUrl":"https://doi.org/10.1556/004.2022.00034","url":null,"abstract":"<p><p>Melarsomine is used intramuscularly to destroy adult heartworms when treating canine heartworm disease (HWD). This drug is highly irritative and can elicit local complications. Therefore, melarsomine should be injected into the paralumbar muscles by strictly adhering to the manufacturers' prescriptions. However, it is not known how to determine the optimal location of the needle during the injection process. Ultrasonography (US) of the epaxial (paralumbar) musculature was used as a new method to measure the cross-sectional diameter of the paralumbar musculature, to determine the required location of the injection needle, and to study the local side effects in two dogs with HWD. The macroscopic appearance of the melarsomine solution during injection was demonstrated by video imaging. Melarsomine was not fully gravitating, but its majority was spreading along the thickest fascia of the musculature. Three minutes thereafter, no ultrasound signs of the melarsomine solution were seen, suggesting a full absorption at least ultrasonographically. This procedure was simulated in vitro with methylene blue solution having the same appearance. Removing the injection needle only after 5 min post-injection could prevent undesirable leakage of the drug through the injection channel into the subcutaneous tissue. Ultrasonography can be a useful aid during the treatment of HWD with melarsomine according to this preliminary study.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":"70 4","pages":"263-268"},"PeriodicalIF":0.9,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9181077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Karolína Sedláčková, Jiřina Száková, Martina Načeradská, Lukáš Praus, Pavel Tlustoš
{"title":"Essential microelement (copper, selenium, zinc) status according to age and sex in healthy cats.","authors":"Karolína Sedláčková, Jiřina Száková, Martina Načeradská, Lukáš Praus, Pavel Tlustoš","doi":"10.1556/004.2022.00036","DOIUrl":"https://doi.org/10.1556/004.2022.00036","url":null,"abstract":"<p><p>Although domestic cats are one of the most popular companion animals, current knowledge on the fate of micronutrients in cats according to their age, sex, and health is very limited. In this study, 72 whole blood and 54 plasma samples from cats of different ages and sex were collected at three veterinary offices in the Czech Republic, and the copper (Cu), selenium (Se), and zinc (Zn) concentrations were determined using inductively coupled plasma mass spectrometry (ICP-MS). The results showed that Cu was significantly (P < 0.05) higher in both plasma and whole blood of males (980 and 958 μg L-1 in plasma and whole blood, respectively) than in females (741 and 766 μg L-1 in plasma and whole blood, respectively), whereas no significant differences between males and females were found for Se and Zn. Similarly, no significant differences were recorded for any of the three elements according to age, although animals older than 7 years tended to have lower plasma concentrations of all three elements. Hypertrophic cardiomyopathy (HCM) is one of the most prevalent diseases of domestic cats. The potential relationship between the essential microelement status in the blood of cats with HCM vs. cats with no clinical signs of HCM was taken into account, but the limited number of HCM-positive individuals did not allow any clear conclusion. Thus, the potential relationships between micronutrient status in cats and the incidence of HCM should be elucidated in further research.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":"70 4","pages":"296-304"},"PeriodicalIF":0.9,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10623588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Efe Kurtdede, Arif Kurtdede, Sibel Yasa Duru, Özkan Duru, Erdal Kara, Yaşar Aluç, Ufuk Kaya
{"title":"Effects of clinoptilolite on heavy metal levels in milk, proinflammatory cytokine responses (IL-1β and IL-6) and oxidative stress in dairy cows.","authors":"Efe Kurtdede, Arif Kurtdede, Sibel Yasa Duru, Özkan Duru, Erdal Kara, Yaşar Aluç, Ufuk Kaya","doi":"10.1556/004.2022.00035","DOIUrl":"https://doi.org/10.1556/004.2022.00035","url":null,"abstract":"<p><p>The effects of clinoptilolite on milk copper (Cu), lead (Pb), zinc (Zn), cadmium (Cd) and iron (Fe) concentrations, proinflammatory cytokine responses, oxidative stress status, whole blood cell counts and liver and kidney functions were investigated in dairy cows exhibiting no signs of any kind of toxicity. Clinoptilolite was added to the feed at a dose of 200 mg kg-1 body weight in the clinoptilolite-treated group (n = 14), but was not added to the feed in the control group (n = 7). In the milk samples (n = 21) collected before the experiment, the Cu, Pb, Zn, Cd and Fe values were 0.021 ± 0.020, 0.104 ± 0.01, 3.42 ± 0.32, <0.000, 0.56 ± 0.34 ppm, respectively. At the end of the experiment (30th day), among the elements measured in milk samples collected from the clinoptilolite-treated group, only the Pb value (0.076 ± 0.01) was lower than the 0-day value of the clinoptilolite-treated group (0.104 ± 0.01) and the 30th-day value of the control group (0.105 ± 0.01) was found to be statistically lower. Changes determined at the end of clinoptilolite application in serum superoxide dismutase (SOD), malondialdehyde (MDA), albumin, glucose, urea and urine creatinine/urine total protein (uCr/uTP) values, which were interpreted as the effect of lead exposure before the trial, were evaluated as the positive effect of clinoptilolite. It was concluded that the addition of clinoptilolite to the feed in dairy cows caused a significant decrease in the amount of Pb in milk, and positive changes in the parameters related to oxidative stress in serum and in parameters related to renal function.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":"70 4","pages":"313-320"},"PeriodicalIF":0.9,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10623589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}