Acta veterinaria Hungarica最新文献

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New record of Tylodelphys metacercariae (Diplostomidae) from Perccottus glenii (Odontobutidae) and their phylogenetic assessment. 刺毛蝗科叶藻囊蚴新记录及其系统发育评价。
IF 0.9 4区 农林科学
Acta veterinaria Hungarica Pub Date : 2022-12-15 DOI: 10.1556/004.2022.00031
Sergey G Sokolov, Peimin Yang, Daria I Lebedeva
{"title":"New record of Tylodelphys metacercariae (Diplostomidae) from Perccottus glenii (Odontobutidae) and their phylogenetic assessment.","authors":"Sergey G Sokolov,&nbsp;Peimin Yang,&nbsp;Daria I Lebedeva","doi":"10.1556/004.2022.00031","DOIUrl":"https://doi.org/10.1556/004.2022.00031","url":null,"abstract":"<p><p>Metacercariae of Tylodelphys sp. were found in the abdominal cavity of the Chinese sleeper (Perccottus glenii) collected in Liaoning Province and Inner Mongolia Autonomous Region of China. The sequences of the mitochondrial cox1 gene and ribosomal ITS1-5.8S rDNA-ITS2 region were obtained and used for molecular identification and phylogenetic assessment of this parasite species. Results of phylogenetic analyses based on ITS and cox1 markers showed that the metacercariae of Tylodelphys sp. ex P. glenii from China were conspecific with specimens of Tylodelphys sp. collected by Sokolov et al. (2013) from the same fish-host species captured earlier in West Siberia, Russia. The examined Tylodelphys sp. ex. P. glenii is the only member of the genus whose metacercariae parasitise the abdominal cavity of fish in northern Eurasia. Tylodelphys sp. ex P. glenii clustered with T. darbyi, T. immer, T. podicipina, and Tylodelphys sp. of Soldánová et al., 2017 based on mitochondrial DNA markers, and with T. darbyi, T. immer, T. kuerepus, and T. schreuringi using nuclear DNA markers.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":"70 4","pages":"274-281"},"PeriodicalIF":0.9,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10617665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Comparison of the short-term complications of TTA-rapid and modified cTTA procedures. 快速和改良cTTA手术短期并发症的比较。
IF 0.9 4区 农林科学
Acta veterinaria Hungarica Pub Date : 2022-12-15 DOI: 10.1556/004.2022.00033
Dorottya Zólyomi, Tamás Ipolyi, Péter Molnár, Márton Papp, Ferenc Szalay, Tibor Németh
{"title":"Comparison of the short-term complications of TTA-rapid and modified cTTA procedures.","authors":"Dorottya Zólyomi,&nbsp;Tamás Ipolyi,&nbsp;Péter Molnár,&nbsp;Márton Papp,&nbsp;Ferenc Szalay,&nbsp;Tibor Németh","doi":"10.1556/004.2022.00033","DOIUrl":"https://doi.org/10.1556/004.2022.00033","url":null,"abstract":"<p><p>The objective of this retrospective study was to determine the complications of the first 30 tibial tuberosity advancement rapid (TTA-rapid) and 30 modified circular tibial tuberosity advancement (mcTTA) procedures performed by our team, and to compare the results with the findings reported in the literature. Our research was based on 30 procedures in each group. All dogs were client-owned. Data were collected only for the study of cases that had a minimum follow-up period of 3 months. Intraoperative (IO) and postoperative (PO) complications were assessed, with the latter divided into two subgroups: major and minor. Results obtained for the TTA-rapid group: IO complications 23.3% (7/30), major PO complications 13.3% (4/30), minor PO complications 16.7% (5/30). Results of the mcTTA group: IO complications 0% (0/30), major PO complications 3.3% (1/30), minor PO complications 20% (6/30). Comparing the complication rates, we found that there was a significant difference between the two groups in the occurrence of IO complications (P = 0.01054); however, there was no significant difference in the incidence of major (P = 0.3533) and minor (P > 0.9999) PO complications between groups. Our results are consistent with the findings reported in the literature and suggest that both techniques are efficient and carry a relatively low complication rate.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":"70 4","pages":"305-312"},"PeriodicalIF":0.9,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10680024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Partial genetic characterisation of a novel alloherpesvirus detected by PCR in a farmed wels catfish (Silurus glanis). 用聚合酶链反应(PCR)在养殖鲇鱼(Silurus glanis)中检测到的一种新型异体疱疹病毒的部分遗传特征。
IF 0.9 4区 农林科学
Acta veterinaria Hungarica Pub Date : 2022-12-15 DOI: 10.1556/004.2022.00038
Zoltán László Tarján, Andor Doszpoly, Edit Eszterbauer, Mária Benkő
{"title":"Partial genetic characterisation of a novel alloherpesvirus detected by PCR in a farmed wels catfish (Silurus glanis).","authors":"Zoltán László Tarján,&nbsp;Andor Doszpoly,&nbsp;Edit Eszterbauer,&nbsp;Mária Benkő","doi":"10.1556/004.2022.00038","DOIUrl":"https://doi.org/10.1556/004.2022.00038","url":null,"abstract":"<p><p>By a broad-range PCR, we detected a novel herpesvirus (HV) in the specimen of a wels catfish (Silurus glanis) presenting disseminated, carp pox-like dermal lesions all over its body. The sequence analysis of the 463-bp PCR product from the viral DNA polymerase gene indicated the presence of a hitherto unknown virus, a putative member of the family Alloherpesviridae in the sample. Another PCR, targeting the terminase gene of fish HVs, provided an additional genomic fragment of over 1,000 bp. Surprisingly, the sequence of a co-amplified, off-target PCR product revealed its origin from a putative gene homologous to ORF87 and ORF45 of cyprinid HVs and anguillid herpesvirus 1 (AngHV-1), respectively. With specific primers, designed according to the genomic maps of the cyprinid and anguillid HVs, a genomic fragment of 15 kb was also amplified and sequenced by primer walking. In phylogeny inferences, based on several genes, the putative wels catfish HV clustered closest to various cyprinid HVs or to AngHV-1. The novel virus, named as silurid herpesvirus 2, represents a distinct species in the genus Cyprinivirus. However, its association with the skin disease remains unclear.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":"70 4","pages":"321-327"},"PeriodicalIF":0.9,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9193397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Na+/K+-ATPase and bone morphogenetic protein-2 expressions in parenchymal and microenvironmental cells of canine mammary tumours. Na+/K+- atp酶和骨形态发生蛋白2在犬乳腺肿瘤实质细胞和微环境细胞中的表达。
IF 0.9 4区 农林科学
Acta veterinaria Hungarica Pub Date : 2022-12-15 DOI: 10.1556/004.2022.00037
Ozlem Ozmen
{"title":"Na+/K+-ATPase and bone morphogenetic protein-2 expressions in parenchymal and microenvironmental cells of canine mammary tumours.","authors":"Ozlem Ozmen","doi":"10.1556/004.2022.00037","DOIUrl":"https://doi.org/10.1556/004.2022.00037","url":null,"abstract":"<p><p>The most common canine tumour is mammary tumour, which resembles breast cancer in humans. Microenvironment is a crucial factor in the formation of breast cancers. In order to distinguish between benign and malignant canine mammary tumours, this study looked at the immunohistochemical expression of Na+/K+-ATPase and bone morphogenetic protein-2 (BMP-2) in tumour and microenvironmental cells. The aim of this study was to evaluate the expression of Na+/K+-ATPase and BMP-2 in canine mammary tumours and their relationship with malignancy. In this investigation, 10 normal breast tissues were used as controls, and 28 benign and 46 malignant mammary tumours were taken from the archives of the Department of Pathology. The findings showed that malignant tumours expressed more Na+/K+-ATPase and BMP-2 than did normal breast tissue. Both markers had a negative or slight expression in benign tumours, whereas they considerably increased in malignant tumours. Both tumour parenchymal and microenvironmental cells in malignancies expressed Na+/K+-ATPase and BMP-2. Na+/K+-ATPase expression was observed to be more prominent in cells when compared to BMP-2. These findings also suggest that Na+/K+-ATPase and BMP-2 could be employed in the future to help diagnose canine and possibly human breast cancers earlier or as possible targets for treatment.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":"70 4","pages":"287-295"},"PeriodicalIF":0.9,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10623590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Annual and seasonal trends in cow's milk quality determined by FT-MIR spectroscopy in Hungary between 2011 and 2020. 2011 年至 2020 年匈牙利通过傅立叶变换红外光谱测定的牛奶质量的年度和季节趋势。
IF 0.9 4区 农林科学
Acta veterinaria Hungarica Pub Date : 2022-08-29 DOI: 10.1556/004.2022.00019
Róbert Kocsis, Judit Süle, Péter Nagy, Judit Gál, Emília Tardy, Gábor Császár, Bence Rácz
{"title":"Annual and seasonal trends in cow's milk quality determined by FT-MIR spectroscopy in Hungary between 2011 and 2020.","authors":"Róbert Kocsis, Judit Süle, Péter Nagy, Judit Gál, Emília Tardy, Gábor Császár, Bence Rácz","doi":"10.1556/004.2022.00019","DOIUrl":"10.1556/004.2022.00019","url":null,"abstract":"<p><p>We analysed and monitored the major chemical composition of cow's bulk milk by Fourier transform mid-infrared (FT-MIR) spectroscopy over a 10-year period in the whole territory of Hungary. In addition, the two most important key parameters for milk quality assessment, total bacterial count (TBC) and somatic cell count (SCC) were also followed. Production parameters showed significant seasonal and yearly changes. The overall mean fat, protein, lactose and solids-non-fat (SNF) contents of cow's milk were 3.81%, 3.32%, 4.74% and 8.76%, respectively. A circannual variation was observed in the chemical composition and yield of milk components of samples examined between 2011 and 2020. Concerning milk fat, milk protein and SNF, the values were the lowest in summer and the highest in winter. In the case of lactose, the minimum values were measured in autumn and the maximum values in spring. An obvious trend of long-term elevation of lactose and SNF was found in the raw cow milk samples over the observed period. The overall mean TBC and SCC of cow's milk were 52 × 103 CFU ml-1 and 270 × 103 cells/ml, respectively. Although there were differences in the monthly average values, no seasonal cyclicality was observed.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2022-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33445499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biomechanical testing of canine tibiae: Changes resulting from different tibial tuberosity advancement techniques - Pilot study. 犬胫骨的生物力学测试:不同的胫骨结节推进技术带来的变化 - 试验研究。
IF 0.9 4区 农林科学
Acta veterinaria Hungarica Pub Date : 2022-08-29 DOI: 10.1556/004.2022.00023
Dorottya Zólyomi, Tamás Ipolyi, Péter Molnár, Tibor Németh, Dénes Faragó, Rita Kiss, Ferenc Szalay
{"title":"Biomechanical testing of canine tibiae: Changes resulting from different tibial tuberosity advancement techniques - Pilot study.","authors":"Dorottya Zólyomi, Tamás Ipolyi, Péter Molnár, Tibor Németh, Dénes Faragó, Rita Kiss, Ferenc Szalay","doi":"10.1556/004.2022.00023","DOIUrl":"10.1556/004.2022.00023","url":null,"abstract":"<p><p>The objective of the present pilot study was to determine the force required to break (a) intact canine tibiae, (b) tibiae following the osteotomy of the tibial tuberosity and (c) tibiae following Tibial Tuberosity Advancement- (TTA-) rapid surgery. Six pairs of tibiae of dogs between 15 and 35 kg body weight were used in a cadaver study. Three groups were created with four tibiae in each group; intact (Group 1), osteotomy of the tibial tuberosity and tibial crest (Group 2) and TTA-rapid (Group 3). The tibiae were put under static axial compressive load, applied until failure. The force required to break the tibiae was termed maximal force (F max). The mean of F max was 8193.25 ± 2082.84 N in Group 1, 6868.58 ± 1950.44 N in Group 2 and 7169.71 ± 4450.39 N in Group 3. The sample size was small for a statistical analysis but as a preliminary result, we have determined the force (F max) required to break canine tibiae. Furthermore, we hypothesise that osteotomies result in weakening of the tibial structure.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2022-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33445500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mucopolysaccharidosis VI in a European Shorthair cat: Neurological presentation, computed tomography findings and genetic investigation. 欧洲短毛猫粘多糖病 VI:神经系统表现、计算机断层扫描结果和基因调查。
IF 0.9 4区 农林科学
Acta veterinaria Hungarica Pub Date : 2022-08-29 DOI: 10.1556/004.2022.00024
Beatrice Bravaccini, Valentina Buffagni, Linda Negro, Giovanna Bertolini, Evelina Burbaite, Marika Menchetti
{"title":"Mucopolysaccharidosis VI in a European Shorthair cat: Neurological presentation, computed tomography findings and genetic investigation.","authors":"Beatrice Bravaccini, Valentina Buffagni, Linda Negro, Giovanna Bertolini, Evelina Burbaite, Marika Menchetti","doi":"10.1556/004.2022.00024","DOIUrl":"10.1556/004.2022.00024","url":null,"abstract":"<p><p>The present case report describes the clinical signs of a 10-month-old, intact female, Domestic Shorthair cat presented with a history of chronic progressive difficulty to walk with the four limbs. The physical and neurological examinations revealed skeletal deformities, corneal opacity and a severe spastic non-ambulatory tetraparesis. Complete blood count and biochemistry profiles were unremarkable. Diffuse bone rarefaction, hyperostosis and an apparent fusion of the vertebral bodies were observed on spinal radiographs. A non-contrast computed tomography (CT) exam of the whole body of the patient was performed. Based on the medical history, clinical findings, laboratory analysis, spinal radiographs and CT findings, a lysosomal storage disorder was suspected. Genetic testing for mucopolysaccharidosis VI and VII revealed a genetic mutation, ARSB variant L476P, confirming the diagnosis of mucopolysaccharidosis type VI.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2022-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33445565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Zoonotic ecotype-I of Anaplasma phagocytophilum in sympatric wildcat, pine marten and red squirrel - Short communication. 同栖野猫、松貂和红松鼠中的噬细胞嗜血杆菌人畜共患病生态型--短篇通讯。
IF 0.9 4区 农林科学
Acta veterinaria Hungarica Pub Date : 2022-08-26 DOI: 10.1556/004.2022.00021
Sándor Hornok, Sándor A Boldogh, Nóra Takács, Attila D Sándor, Barbara Tuska-Szalay
{"title":"Zoonotic ecotype-I of Anaplasma phagocytophilum in sympatric wildcat, pine marten and red squirrel - Short communication.","authors":"Sándor Hornok, Sándor A Boldogh, Nóra Takács, Attila D Sándor, Barbara Tuska-Szalay","doi":"10.1556/004.2022.00021","DOIUrl":"10.1556/004.2022.00021","url":null,"abstract":"<p><p>Anaplasma phagocytophilum is the causative agent of granulocytic anaplasmosis in humans, dogs, cats, horses and tick-borne fever in ruminants. In Europe, its main vector is the tick species Ixodes ricinus. In this study, spleen and liver samples, as well as ticks from 18 wild-living mammals (belonging to seven species) were analysed for the presence of A. phagocytophilum with molecular methods. The zoonotic ecotype-I of A. phagocytophilum was identified in a European wildcat (Felis silvestris) and its tick, a European pine marten (Martes martes) and a Eurasian red squirrel (Sciurus vulgaris). All PCR-positive samples were collected in 2019 and originated in the same geographic area. These results indicate that taxonomically diverse mammalian species can maintain the local enzootic cycle of the same genotype of A. phagocytophilum. To the best of our knowledge, this is the first report of the zoonotic variant of A. phagocytophilum in the wildcat and in the European pine marten in a broad geographical context, as well as in the red squirrel in Hungary. Since all these host species are well known for their urban and peri-urban presence, the results of this study verify their role in the synanthropic enzootic cycle of granulocytic anaplasmosis and tick-borne fever.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2022-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33440154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Paraquat modulates immunological function in bone marrow-derived macrophages. 百草枯能调节骨髓巨噬细胞的免疫功能
IF 0.9 4区 农林科学
Acta veterinaria Hungarica Pub Date : 2022-03-09 DOI: 10.1556/004.2022.00003
Piyarat Srinontong, Jaroon Wandee, Worapol Aengwanich
{"title":"Paraquat modulates immunological function in bone marrow-derived macrophages.","authors":"Piyarat Srinontong, Jaroon Wandee, Worapol Aengwanich","doi":"10.1556/004.2022.00003","DOIUrl":"https://doi.org/10.1556/004.2022.00003","url":null,"abstract":"<p><p>The herbicide paraquat (PQ) is known to affect the immune system. Many reports have indicated that PQ impacts on the viability and functions of the immune cells, however, the underlying mechanism in detail is still unknown. The aim of this study was to evaluate the effects of PQ on the free radical production, oxidative stress, cell death and pro-inflammatory gene expression of murine bone marrow-derived macrophages (BMDMs) from C57BL/6NJcl mice in vitro. BMDMs were incubated with PQ at 0, 200 and 400 µM concentrations for 24 h. Intracellular reactive oxygen species (ROS) production, apoptosis, cell viability, nitric oxide, inducible nitric oxide synthase (iNOS), and IL-6 expression levels were measured. The results revealed that PQ treatments led to a decrease in the cell viability and induced apoptotic cell death in a dose-dependent manner. Additionally, PQ also induced the generation of ROS. The mRNA level of the pro-inflammatory mediator genes iNOS and IL-6 were also elevated, while the level of lipid peroxide (malondialdehyde) production remained unaltered. Interestingly, the PQ treatment led to a decrease in the nitric oxide production. These results indicate that the increased cellular ROS production, due to the PQ treatment, induces apoptosis and the herbicide triggers production of iNOS and IL-6 in BMDMs.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2022-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139904777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Are owned dogs or stray dogs more prepared to diseases? A comparative study of immune system gene expression of perforin and granzymes. 养狗和流浪狗对疾病的抵抗力更强吗?穿孔素与颗粒酶免疫系统基因表达的比较研究。
IF 0.9 4区 农林科学
Acta veterinaria Hungarica Pub Date : 2022-03-02 DOI: 10.1556/004.2022.00005
Mehmet Cevat Temizkan, Gonca Sonmez
{"title":"Are owned dogs or stray dogs more prepared to diseases? A comparative study of immune system gene expression of perforin and granzymes.","authors":"Mehmet Cevat Temizkan, Gonca Sonmez","doi":"10.1556/004.2022.00005","DOIUrl":"10.1556/004.2022.00005","url":null,"abstract":"<p><p>Stray dogs are inevitably exposed to more infections than owned dogs living indoor. However, no studies have investigated whether the immune system gene expression differs between owned dogs kept in better care conditions and stray dogs living outside. To investigate this, blood samples were taken from 90 dogs (45 owned and 45 stray dogs) that were checked and confirmed as healthy. By using qPCR, the amples were analyzed for the expression of the perforin, granzyme A and granzyme B genes, which are associated with the activation of apoptotic pathways in the immune system. We found that the perforin and granzyme A gene expression levels were higher in stray dogs although the differences were not statistically significant. On the other hand, a 2.81 times higher and a notable difference (P < 0.001) was found in the expression level of granzyme B gene in stray dogs. These results indicate that the immune system in stray dogs might be more prepared for diseases than that of the owned dogs and the granzyme B gene plays a more dominant role in the immune response than granzyme A and perforin.</p>","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":"1 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2022-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42879125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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