Acta veterinaria Hungarica最新文献_第8页

Partial genetic characterisation of a novel alloherpesvirus detected by PCR in a farmed wels catfish (Silurus glanis). 用聚合酶链反应(PCR)在养殖鲇鱼(Silurus glanis)中检测到的一种新型异体疱疹病毒的部分遗传特征。

IF 0.9 4区农林科学

Acta veterinaria Hungarica Pub Date : 2022-12-15 DOI: 10.1556/004.2022.00038

Zoltán László Tarján, Andor Doszpoly, Edit Eszterbauer, Mária Benkő

{"title":"Partial genetic characterisation of a novel alloherpesvirus detected by PCR in a farmed wels catfish (Silurus glanis).","authors":"Zoltán László Tarján, Andor Doszpoly, Edit Eszterbauer, Mária Benkő","doi":"10.1556/004.2022.00038","DOIUrl":"https://doi.org/10.1556/004.2022.00038","url":null,"abstract":"By a broad-range PCR, we detected a novel herpesvirus (HV) in the specimen of a wels catfish (Silurus glanis) presenting disseminated, carp pox-like dermal lesions all over its body. The sequence analysis of the 463-bp PCR product from the viral DNA polymerase gene indicated the presence of a hitherto unknown virus, a putative member of the family Alloherpesviridae in the sample. Another PCR, targeting the terminase gene of fish HVs, provided an additional genomic fragment of over 1,000 bp. Surprisingly, the sequence of a co-amplified, off-target PCR product revealed its origin from a putative gene homologous to ORF87 and ORF45 of cyprinid HVs and anguillid herpesvirus 1 (AngHV-1), respectively. With specific primers, designed according to the genomic maps of the cyprinid and anguillid HVs, a genomic fragment of 15 kb was also amplified and sequenced by primer walking. In phylogeny inferences, based on several genes, the putative wels catfish HV clustered closest to various cyprinid HVs or to AngHV-1. The novel virus, named as silurid herpesvirus 2, represents a distinct species in the genus Cyprinivirus. However, its association with the skin disease remains unclear.","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":"70 4","pages":"321-327"},"PeriodicalIF":0.9,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9193397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Na+/K+-ATPase and bone morphogenetic protein-2 expressions in parenchymal and microenvironmental cells of canine mammary tumours. Na+/K+- atp酶和骨形态发生蛋白2在犬乳腺肿瘤实质细胞和微环境细胞中的表达。

IF 0.9 4区农林科学

Acta veterinaria Hungarica Pub Date : 2022-12-15 DOI: 10.1556/004.2022.00037

Ozlem Ozmen

{"title":"Na+/K+-ATPase and bone morphogenetic protein-2 expressions in parenchymal and microenvironmental cells of canine mammary tumours.","authors":"Ozlem Ozmen","doi":"10.1556/004.2022.00037","DOIUrl":"https://doi.org/10.1556/004.2022.00037","url":null,"abstract":"The most common canine tumour is mammary tumour, which resembles breast cancer in humans. Microenvironment is a crucial factor in the formation of breast cancers. In order to distinguish between benign and malignant canine mammary tumours, this study looked at the immunohistochemical expression of Na+/K+-ATPase and bone morphogenetic protein-2 (BMP-2) in tumour and microenvironmental cells. The aim of this study was to evaluate the expression of Na+/K+-ATPase and BMP-2 in canine mammary tumours and their relationship with malignancy. In this investigation, 10 normal breast tissues were used as controls, and 28 benign and 46 malignant mammary tumours were taken from the archives of the Department of Pathology. The findings showed that malignant tumours expressed more Na+/K+-ATPase and BMP-2 than did normal breast tissue. Both markers had a negative or slight expression in benign tumours, whereas they considerably increased in malignant tumours. Both tumour parenchymal and microenvironmental cells in malignancies expressed Na+/K+-ATPase and BMP-2. Na+/K+-ATPase expression was observed to be more prominent in cells when compared to BMP-2. These findings also suggest that Na+/K+-ATPase and BMP-2 could be employed in the future to help diagnose canine and possibly human breast cancers earlier or as possible targets for treatment.","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":"70 4","pages":"287-295"},"PeriodicalIF":0.9,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10623590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Annual and seasonal trends in cow's milk quality determined by FT-MIR spectroscopy in Hungary between 2011 and 2020. 2011 年至 2020 年匈牙利通过傅立叶变换红外光谱测定的牛奶质量的年度和季节趋势。

IF 0.9 4区农林科学

Acta veterinaria Hungarica Pub Date : 2022-08-29 DOI: 10.1556/004.2022.00019

Róbert Kocsis, Judit Süle, Péter Nagy, Judit Gál, Emília Tardy, Gábor Császár, Bence Rácz

{"title":"Annual and seasonal trends in cow's milk quality determined by FT-MIR spectroscopy in Hungary between 2011 and 2020.","authors":"Róbert Kocsis, Judit Süle, Péter Nagy, Judit Gál, Emília Tardy, Gábor Császár, Bence Rácz","doi":"10.1556/004.2022.00019","DOIUrl":"10.1556/004.2022.00019","url":null,"abstract":"We analysed and monitored the major chemical composition of cow's bulk milk by Fourier transform mid-infrared (FT-MIR) spectroscopy over a 10-year period in the whole territory of Hungary. In addition, the two most important key parameters for milk quality assessment, total bacterial count (TBC) and somatic cell count (SCC) were also followed. Production parameters showed significant seasonal and yearly changes. The overall mean fat, protein, lactose and solids-non-fat (SNF) contents of cow's milk were 3.81%, 3.32%, 4.74% and 8.76%, respectively. A circannual variation was observed in the chemical composition and yield of milk components of samples examined between 2011 and 2020. Concerning milk fat, milk protein and SNF, the values were the lowest in summer and the highest in winter. In the case of lactose, the minimum values were measured in autumn and the maximum values in spring. An obvious trend of long-term elevation of lactose and SNF was found in the raw cow milk samples over the observed period. The overall mean TBC and SCC of cow's milk were 52 × 103 CFU ml-1 and 270 × 103 cells/ml, respectively. Although there were differences in the monthly average values, no seasonal cyclicality was observed.","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2022-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33445499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomechanical testing of canine tibiae: Changes resulting from different tibial tuberosity advancement techniques - Pilot study. 犬胫骨的生物力学测试：不同的胫骨结节推进技术带来的变化 - 试验研究。

IF 0.9 4区农林科学

Acta veterinaria Hungarica Pub Date : 2022-08-29 DOI: 10.1556/004.2022.00023

Dorottya Zólyomi, Tamás Ipolyi, Péter Molnár, Tibor Németh, Dénes Faragó, Rita Kiss, Ferenc Szalay

引用次数: 0

Mucopolysaccharidosis VI in a European Shorthair cat: Neurological presentation, computed tomography findings and genetic investigation. 欧洲短毛猫粘多糖病 VI：神经系统表现、计算机断层扫描结果和基因调查。

IF 0.9 4区农林科学

Acta veterinaria Hungarica Pub Date : 2022-08-29 DOI: 10.1556/004.2022.00024

Beatrice Bravaccini, Valentina Buffagni, Linda Negro, Giovanna Bertolini, Evelina Burbaite, Marika Menchetti

引用次数: 0

Zoonotic ecotype-I of Anaplasma phagocytophilum in sympatric wildcat, pine marten and red squirrel - Short communication. 同栖野猫、松貂和红松鼠中的噬细胞嗜血杆菌人畜共患病生态型--短篇通讯。

IF 0.9 4区农林科学

Acta veterinaria Hungarica Pub Date : 2022-08-26 DOI: 10.1556/004.2022.00021

Sándor Hornok, Sándor A Boldogh, Nóra Takács, Attila D Sándor, Barbara Tuska-Szalay

{"title":"Zoonotic ecotype-I of Anaplasma phagocytophilum in sympatric wildcat, pine marten and red squirrel - Short communication.","authors":"Sándor Hornok, Sándor A Boldogh, Nóra Takács, Attila D Sándor, Barbara Tuska-Szalay","doi":"10.1556/004.2022.00021","DOIUrl":"10.1556/004.2022.00021","url":null,"abstract":"Anaplasma phagocytophilum is the causative agent of granulocytic anaplasmosis in humans, dogs, cats, horses and tick-borne fever in ruminants. In Europe, its main vector is the tick species Ixodes ricinus. In this study, spleen and liver samples, as well as ticks from 18 wild-living mammals (belonging to seven species) were analysed for the presence of A. phagocytophilum with molecular methods. The zoonotic ecotype-I of A. phagocytophilum was identified in a European wildcat (Felis silvestris) and its tick, a European pine marten (Martes martes) and a Eurasian red squirrel (Sciurus vulgaris). All PCR-positive samples were collected in 2019 and originated in the same geographic area. These results indicate that taxonomically diverse mammalian species can maintain the local enzootic cycle of the same genotype of A. phagocytophilum. To the best of our knowledge, this is the first report of the zoonotic variant of A. phagocytophilum in the wildcat and in the European pine marten in a broad geographical context, as well as in the red squirrel in Hungary. Since all these host species are well known for their urban and peri-urban presence, the results of this study verify their role in the synanthropic enzootic cycle of granulocytic anaplasmosis and tick-borne fever.","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2022-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33440154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paraquat modulates immunological function in bone marrow-derived macrophages. 百草枯能调节骨髓巨噬细胞的免疫功能

IF 0.9 4区农林科学

Acta veterinaria Hungarica Pub Date : 2022-03-09 DOI: 10.1556/004.2022.00003

Piyarat Srinontong, Jaroon Wandee, Worapol Aengwanich

{"title":"Paraquat modulates immunological function in bone marrow-derived macrophages.","authors":"Piyarat Srinontong, Jaroon Wandee, Worapol Aengwanich","doi":"10.1556/004.2022.00003","DOIUrl":"https://doi.org/10.1556/004.2022.00003","url":null,"abstract":"The herbicide paraquat (PQ) is known to affect the immune system. Many reports have indicated that PQ impacts on the viability and functions of the immune cells, however, the underlying mechanism in detail is still unknown. The aim of this study was to evaluate the effects of PQ on the free radical production, oxidative stress, cell death and pro-inflammatory gene expression of murine bone marrow-derived macrophages (BMDMs) from C57BL/6NJcl mice in vitro. BMDMs were incubated with PQ at 0, 200 and 400 µM concentrations for 24 h. Intracellular reactive oxygen species (ROS) production, apoptosis, cell viability, nitric oxide, inducible nitric oxide synthase (iNOS), and IL-6 expression levels were measured. The results revealed that PQ treatments led to a decrease in the cell viability and induced apoptotic cell death in a dose-dependent manner. Additionally, PQ also induced the generation of ROS. The mRNA level of the pro-inflammatory mediator genes iNOS and IL-6 were also elevated, while the level of lipid peroxide (malondialdehyde) production remained unaltered. Interestingly, the PQ treatment led to a decrease in the nitric oxide production. These results indicate that the increased cellular ROS production, due to the PQ treatment, induces apoptosis and the herbicide triggers production of iNOS and IL-6 in BMDMs.","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2022-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139904777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Are owned dogs or stray dogs more prepared to diseases? A comparative study of immune system gene expression of perforin and granzymes. 养狗和流浪狗对疾病的抵抗力更强吗?穿孔素与颗粒酶免疫系统基因表达的比较研究。

IF 0.9 4区农林科学

Acta veterinaria Hungarica Pub Date : 2022-03-02 DOI: 10.1556/004.2022.00005

Mehmet Cevat Temizkan, Gonca Sonmez

引用次数: 0

Corrigendum: Determination and molecular analysis of antibiotic resistance in Gram-negative enteric bacteria isolated from Pelophylax sp. in the Eastern Black Sea Region. 更正：从东黑海地区的黄颡鱼中分离出的革兰氏阴性肠道细菌的抗生素耐药性测定和分子分析。

IF 0.9 4区农林科学

Acta veterinaria Hungarica Pub Date : 2022-02-07 DOI: 10.1556/004.2022.10039

Erva Rakici, Abdullah Altunisik, Kazim Sahin, Osman Birol Ozgumus

引用次数: 0

Ecology and prevalence of Borrelia burgdorferi s.l. in Ixodes ricinus (Acari: Ixodidae) ticks. Ixodes ricinus (Acari: Ixodidae) 蜱中 Borrelia burgdorferi s.l. 的生态学和流行率。

IF 0.9 4区农林科学

Acta veterinaria Hungarica Pub Date : 2022-02-07 DOI: 10.1556/004.2021.00056

Ivana Ivanović, Marina Žekić Stošić, Eva Ružić Sabljić, Tjaša Cerar Kišek, Vesna Cvitković Špik, Aleksandra Popović, Sara Savić

{"title":"Ecology and prevalence of Borrelia burgdorferi s.l. in Ixodes ricinus (Acari: Ixodidae) ticks.","authors":"Ivana Ivanović, Marina Žekić Stošić, Eva Ružić Sabljić, Tjaša Cerar Kišek, Vesna Cvitković Špik, Aleksandra Popović, Sara Savić","doi":"10.1556/004.2021.00056","DOIUrl":"10.1556/004.2021.00056","url":null,"abstract":"Weather conditions greatly affect tick population densities and activity, on which depends the occurrence of tick-borne diseases (TBDs). During the spring months from 2017 to 2019, 1,357 specimens of Ixodes ricinus ticks were collected at 9 localities in the vicinity of Novi Sad (Serbia). The number of collected ticks varied considerably among the different sampling sites and years. Also, a statistically significant difference was found between months and observed number of ticks for each stadium. By statistical analysis of tick activity depending on microclimatic conditions, a positive and statistically significant relationship between temperature and the number of ticks for each life stage was established, but not for humidity. Dew had a statistically significant impact only on nymphs but not on adults. The infection rate of Borrelia burgdorferi s.l. was the highest in March (46.5-51.2%) and the lowest in May (32.9-34.8%). The highest prevalence was detected in males and the lowest in nymphs. Since there is a positive and statistically significant correlation between tick number and prevalence, the ability to provide weather-based predictions of the seasonal patterns of current tick activity is important for the risk assessment of TBDs such as Lyme borreliosis.","PeriodicalId":7247,"journal":{"name":"Acta veterinaria Hungarica","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2022-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39769256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0