Brain cell biology最新文献

O-GlcNAc modification of radial glial vimentin filaments in the developing chick brain O-GlcNAc对发育中的鸡脑径向胶质蛋白丝的修饰

Brain cell biology Pub Date : 2008-12-01 DOI: 10.1007/s11068-008-9036-5

A. Farach, D. Galileo

引用次数: 23

Brain cell biology Pub Date : 2008-12-01 DOI: 10.1007/s11068-009-9042-2

George J Augustine

引用次数: 0

Increased expression of β-catenin in brain microvessels of a segmentally trisomic (Ts65Dn) mouse model of Down syndrome 部分三体(Ts65Dn)唐氏综合征小鼠脑微血管中β-连环蛋白的表达增加

Brain cell biology Pub Date : 2008-12-01 DOI: 10.1007/s11068-008-9038-3

A. Vorbrodt, Shuyun Li, W. Brown, N. Ramakrishna

引用次数: 11

Differences in c-jun and nNOS expression levels in motoneurons following different kinds of axonal injury in adult rats. 成年大鼠不同类型轴突损伤后运动神经元c-jun和nNOS表达水平的差异

Brain cell biology Pub Date : 2008-12-01 Epub Date: 2009-02-24 DOI: 10.1007/s11068-009-9040-4

Li-Hua Zhou, Shu Han, Yuan-Yun Xie, Lin-Lin Wang, Zhi-Bin Yao

{"title":"Differences in c-jun and nNOS expression levels in motoneurons following different kinds of axonal injury in adult rats.","authors":"Li-Hua Zhou, Shu Han, Yuan-Yun Xie, Lin-Lin Wang, Zhi-Bin Yao","doi":"10.1007/s11068-009-9040-4","DOIUrl":"https://doi.org/10.1007/s11068-009-9040-4","url":null,"abstract":"In the peripheral nervous system (PNS), root avulsion causes motoneuron degeneration, but the majority of motoneurons can survive axotomy. In order to study the mechanism of motoneuron degeneration, we compared the expression patterns of c-jun and neuronal nitric oxide synthase (nNOS), the well-known molecular players in PNS regeneration and degeneration, among adult rats having undergone axotomy (Ax), avulsion (Av), or pre-axotomy plus secondary avulsion (Ax + Av) of the brachial plexus. Our results showed that the highest and longest-lasting c-jun activation occurred in Ax, which was much stronger than those in Av and Ax + Av. The time course and intensity of c-jun expression in Ax + Av were similar to those in Av except on day 1, while the pre-axotomy condition resulted in a transient up-regulation of c-jun to a level comparable to that in Ax. Axotomy alone did not induce nNOS expression in motoneurons. Pre-axotomy left-shifted the time course of nNOS induction in Ax + Av compared to that in Av. Motoneuron loss was not evident in Ax, while it was 70% in Av and more than 85% in Ax + Av at 8 weeks postinjury. The survival of motoneurons was positively correlated with c-jun induction, but not with nNOS expression in motoneurons. Moreover, c-jun induction was negatively correlated with nNOS induction in injured motoneurons. Our results indicate that functional crosstalk between c-jun and nNOS might play an important role in avulsion-induced motoneuron degeneration, while c-jun might act as a prerequisite survival factor and nNOS might act as a predictor for the onset of motoneuron degeneration.","PeriodicalId":72445,"journal":{"name":"Brain cell biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11068-009-9040-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28002414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

Imaging activity of neuronal populations with new long-wavelength voltage-sensitive dyes. 新型长波长电压敏感染料对神经元群成像活性的影响。

Brain cell biology Pub Date : 2008-12-01 Epub Date: 2009-02-14 DOI: 10.1007/s11068-009-9039-x

Michelle Z L Kee, Joseph P Wuskell, Leslie M Loew, George J Augustine, Yuko Sekino

引用次数: 23

Direct interaction of SNARE complex binding protein synaphin/complexin with calcium sensor synaptotagmin 1 SNARE复合物结合蛋白synaphin/complexin与钙传感器synaptotagin 1的直接相互作用

Brain cell biology Pub Date : 2008-12-01 DOI: 10.1007/s11068-008-9032-9

H. Tokumaru, C. Shimizu-Okabe, T. Abe

引用次数: 25

Development of microscopic systems for high-speed dual-excitation ratiometric Ca2+ imaging. 高速双激发比例Ca2+成像显微系统的发展。

Brain cell biology Pub Date : 2008-08-01 Epub Date: 2008-10-22 DOI: 10.1007/s11068-008-9033-8

Takashi Fukano, Satoshi Shimozono, Atsushi Miyawaki

{"title":"Development of microscopic systems for high-speed dual-excitation ratiometric Ca2+ imaging.","authors":"Takashi Fukano, Satoshi Shimozono, Atsushi Miyawaki","doi":"10.1007/s11068-008-9033-8","DOIUrl":"https://doi.org/10.1007/s11068-008-9033-8","url":null,"abstract":"For quantitative measurements of Ca(2+) concentration ([Ca(2+)]), ratiometric dyes are preferable, because the use of such dyes allows for correction of uneven loading or partitioning of dye within the cell as well as variations in cell thickness. Although dual-excitation ratiometric dyes for measuring [Ca(2+)], such as Fura-2, Fura-Red, and ratiometric-pericam, are widely used for a variety of applications, it has been difficult to use them for monitoring very fast Ca(2+) dynamics or Ca(2+) changes in highly motile cells. To overcome this problem, we have developed three new dual-excitation ratiometry systems. (1) A system in which two laser beams are alternated on every scanning line, allowing us to obtain confocal images using dual-excitation ratiometric dyes. This system increases the rate at which ratio measurements can be made to 200 Hz and provides confocal images at 1-10 Hz depending on the image size. (2) A truly simultaneous dual-excitation ratiometry system that used linearly polarized excitation light and polarization detection, allowing us to obtain ratiometric images without any time lag. This system, however, is based on statistical features of the fluorescence polarization and is limited to samples that contain a large number of fluorophores. In addition, this method requires complicated calculations. (3) An efficient, nearly simultaneous dual-excitation ratiometry system that allows us to rapidly switch between two synchronized excitation-detection components by employing two high-power light-emitting diodes (LEDs) and two high-speed liquid crystal shutters. The open/close operation of the two shutters is synchronized with the on/off switching of the two LEDs. This system increases the rate at which ratio measurements are made to 1 kHz, and provides ratio images at 10-100 Hz depending on the signal intensity.","PeriodicalId":72445,"journal":{"name":"Brain cell biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11068-008-9033-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27810243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Improved expression of halorhodopsin for light-induced silencing of neuronal activity. 改善光视紫红质在光诱导神经元活动沉默中的表达。

Brain cell biology Pub Date : 2008-08-01 Epub Date: 2008-10-17 DOI: 10.1007/s11068-008-9034-7

Shengli Zhao, Catarina Cunha, Feng Zhang, Qun Liu, Bernd Gloss, Karl Deisseroth, George J Augustine, Guoping Feng

{"title":"Improved expression of halorhodopsin for light-induced silencing of neuronal activity.","authors":"Shengli Zhao, Catarina Cunha, Feng Zhang, Qun Liu, Bernd Gloss, Karl Deisseroth, George J Augustine, Guoping Feng","doi":"10.1007/s11068-008-9034-7","DOIUrl":"https://doi.org/10.1007/s11068-008-9034-7","url":null,"abstract":"The ability to control and manipulate neuronal activity within an intact mammalian brain is of key importance for mapping functional connectivity and for dissecting the neural circuitry underlying behaviors. We have previously generated transgenic mice that express channelrhodopsin-2 for light-induced activation of neurons and mapping of neural circuits. Here we describe transgenic mice that express halorhodopsin (NpHR), a light-driven chloride pump that can be used to silence neuronal activity via light. Using the Thy-1 promoter to target NpHR expression to neurons, we found that neurons in these mice expressed high levels of NpHR-YFP and that illumination of cortical pyramidal neurons expressing NpHR-YFP led to rapid, reversible photoinhibition of action potential firing in these cells. However, NpHR-YFP expression led to the formation of numerous intracellular blebs, which may disrupt neuronal function. Labeling of various subcellular markers indicated that the blebs arise from retention of NpHR-YFP in the endoplasmic reticulum. By improving the signal peptide sequence and adding an ER export signal to NpHR-YFP, we eliminated the formation of blebs and dramatically increased the membrane expression of NpHR-YFP. Thus, the improved version of NpHR should serve as an excellent tool for neuronal silencing in vitro and in vivo.","PeriodicalId":72445,"journal":{"name":"Brain cell biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2008-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s11068-008-9034-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27801344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 199

FRET imaging and in silico simulation: analysis of the signaling network of nerve growth factor-induced neuritogenesis. FRET成像和计算机模拟:神经生长因子诱导神经新生的信号网络分析。

Brain cell biology Pub Date : 2008-08-01 Epub Date: 2008-07-25 DOI: 10.1007/s11068-008-9028-5

Takeshi Nakamura, Kazuhiro Aoki, Michiyuki Matsuda

引用次数: 9

Genetically encoded fluorescent sensors of membrane potential. 基因编码的膜电位荧光传感器。

Brain cell biology Pub Date : 2008-08-01 Epub Date: 2008-08-05 DOI: 10.1007/s11068-008-9026-7

B J Baker, H Mutoh, D Dimitrov, W Akemann, A Perron, Y Iwamoto, L Jin, L B Cohen, E Y Isacoff, V A Pieribone, T Hughes, T Knöpfel

引用次数: 0