BMC genomic dataPub Date : 2024-03-13DOI: 10.1186/s12863-024-01216-y
Fuhua Liu, Yang Zhao, Xiurong Wang, Biao Wang, Feng Xiao, Kequan He
{"title":"Transcriptome analysis reveals regulatory mechanisms of different drought-tolerant Gleditsia sinensis seedlings under drought stress.","authors":"Fuhua Liu, Yang Zhao, Xiurong Wang, Biao Wang, Feng Xiao, Kequan He","doi":"10.1186/s12863-024-01216-y","DOIUrl":"10.1186/s12863-024-01216-y","url":null,"abstract":"<p><strong>Background: </strong>Gleditsia sinensis is a significant tree species from both ecological and economic perspectives. However, its growth is hampered by temporary droughts during the seedling stage, thereby impeding the development of the G. sinensis industry. Drought stress and rehydration of semi-annual potted seedlings using an artificial simulated water control method. RNA sequencing (RNA-seq) analyses were conducted on leaves collected from highly resistant (HR) and highly susceptible (HS) seedling families at five different stages during the process of drought stress and rehydration to investigate their gene expression patterns.</p><p><strong>Results: </strong>The differentially expressed genes (DEGs) were predominantly enriched in pathways related to \"chloroplast\" (GO:0009507), \"photosynthesis\" (GO:0015979), \"plant hormone signal transduction\" (map04075), \"flavonoid biosynthesis\" (map00941), \"stress response\", \"response to reactive oxygen species (ROS)\" (GO:0000302), \"signal transduction\" (GO:0007165) in G. sinensis HR and HS families exposed to mild and severe drought stress. Additionally, the pathways related to \"plant hormone signal transduction\" (map04075), and osmoregulation were also enriched. The difference in drought tolerance between the two families of G. sinensis may be associated with \"transmembrane transporter activity\" (GO:0022857), \"stress response\", \"hormones and signal transduction\" (GO:0007165), \"cutin, suberine and wax biosynthesis\" (map00073), \"ribosome\" (map03010), \"photosynthesis\" (map00195), \"sugar metabolism\", and others. An enrichment analysis of DEGs under severe drought stress suggests that the drought tolerance of both families may be related to \"water-soluble vitamin metabolic process\" (GO:0006767), \"photosynthesis\" (map00195), \"plant hormone signal transduction\" (map04075), \"starch and sucrose metabolism\" (map00500), and \"galactose metabolism\" (map00052). Osmoregulation-related genes such as delta-1-pyrroline-5-carboxylate synthase (P5CS), Amino acid permease (AAP), Amino acid permease 2 (AAP2) and Trehalose-phosphate synthase (TPS), as well as the antioxidant enzyme L-ascorbate peroxidase 6 (APX6), may be significant genes involved in drought tolerance in G. sinensis. Five genes were selected randomly to validate the RNA-seq results using quantitative real-time PCR (RT-qPCR) and they indicated that the transcriptome data were reliable.</p><p><strong>Conclusions: </strong>The study presents information on the molecular regulation of the drought tolerance mechanism in G. sinensis and provides a reference for further research on the molecular mechanisms involved in drought tolerance breeding of G. sinensis.</p>","PeriodicalId":72427,"journal":{"name":"BMC genomic data","volume":"25 1","pages":"29"},"PeriodicalIF":0.0,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10935782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140121519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BMC genomic dataPub Date : 2024-03-08DOI: 10.1186/s12863-024-01211-3
Youfang Sun, Yi Lan, Nils Rädecker, Huaxia Sheng, Guillermo Diaz-Pulido, Pei-Yuan Qian, Hui Huang
{"title":"Gene expression of Pocillopora damicornis coral larvae in response to acidification and ocean warming.","authors":"Youfang Sun, Yi Lan, Nils Rädecker, Huaxia Sheng, Guillermo Diaz-Pulido, Pei-Yuan Qian, Hui Huang","doi":"10.1186/s12863-024-01211-3","DOIUrl":"10.1186/s12863-024-01211-3","url":null,"abstract":"<p><strong>Objectives: </strong>The endosymbiosis with Symbiodiniaceae is key to the ecological success of reef-building corals. However, climate change is threatening to destabilize this symbiosis on a global scale. Most studies looking into the response of corals to heat stress and ocean acidification focus on coral colonies. As such, our knowledge of symbiotic interactions and stress response in other stages of the coral lifecycle remains limited. Establishing transcriptomic resources for coral larvae under stress can thus provide a foundation for understanding the genomic basis of symbiosis, and its susceptibility to climate change. Here, we present a gene expression dataset generated from larvae of the coral Pocillopora damicornis in response to exposure to acidification and elevated temperature conditions below the bleaching threshold of the symbiosis.</p><p><strong>Data description: </strong>This dataset is comprised of 16 samples (30 larvae per sample) collected from four treatments (Control, High pCO<sub>2</sub>, High Temperature, and Combined pCO<sub>2</sub> and Temperature treatments). Freshly collected larvae were exposed to treatment conditions for five days, providing valuable insights into gene expression in this vulnerable stage of the lifecycle. In combination with previously published datasets, this transcriptomic resource will facilitate the in-depth investigation of the effects of ocean acidification and elevated temperature on coral larvae and its implication for symbiosis.</p>","PeriodicalId":72427,"journal":{"name":"BMC genomic data","volume":"25 1","pages":"28"},"PeriodicalIF":0.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10924396/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140066345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BMC genomic dataPub Date : 2024-03-05DOI: 10.1186/s12863-024-01214-0
Ken Kraaijeveld, Koen Bossers, Nikola Petrusevski, Stef Pieterman, Linda G R Bruins-van Sonsbeek, Floyd Wittink
{"title":"ONT read assembly of the black rhino genome.","authors":"Ken Kraaijeveld, Koen Bossers, Nikola Petrusevski, Stef Pieterman, Linda G R Bruins-van Sonsbeek, Floyd Wittink","doi":"10.1186/s12863-024-01214-0","DOIUrl":"10.1186/s12863-024-01214-0","url":null,"abstract":"<p><strong>Objectives: </strong>The black rhinoceros (Diceros bicornis) is an endangered mammal for which a captive breeding program is part of the conservation effort. Black rhinos in zoo's often suffer from chronic infections and heamochromatosis. Furthermore, breeding is hampered by low male fertility. To aid a research project studying these topics, we sequenced and assembled the genome of a captive male black rhino using ONT sequencing data only.</p><p><strong>Data description: </strong>This work produced over 100 Gb whole genome sequencing reads from whole blood. These were assembled into a 2.47 Gb draft genome consisting of 834 contigs with an N50 of 29.53 Mb. The genome annotation was lifted over from an available genome annotation for black rhino, which resulted in the retrieval of over 99% of gene features. This new genome assembly will be a valuable resource in for conservation genetic research in this species.</p>","PeriodicalId":72427,"journal":{"name":"BMC genomic data","volume":"25 1","pages":"27"},"PeriodicalIF":0.0,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10916078/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140041069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BMC genomic dataPub Date : 2024-03-05DOI: 10.1186/s12863-024-01210-4
Mujahid Hussain, Muhammad Mubashar Javed, Adnan Sami, Muhammad Shafiq, Qurban Ali, Hafiz Sabah-Ud-Din Mazhar, Javaria Tabassum, Muhammad Arshad Javed, Muhammad Zeeshan Haider, Muhammad Hussain, Irfan Ali Sabir, Daoud Ali
{"title":"Genome-wide analysis of plant specific YABBY transcription factor gene family in carrot (Dacus carota) and its comparison with Arabidopsis.","authors":"Mujahid Hussain, Muhammad Mubashar Javed, Adnan Sami, Muhammad Shafiq, Qurban Ali, Hafiz Sabah-Ud-Din Mazhar, Javaria Tabassum, Muhammad Arshad Javed, Muhammad Zeeshan Haider, Muhammad Hussain, Irfan Ali Sabir, Daoud Ali","doi":"10.1186/s12863-024-01210-4","DOIUrl":"10.1186/s12863-024-01210-4","url":null,"abstract":"<p><p>YABBY gene family is a plant-specific transcription factor with DNA binding domain involved in various functions i.e. regulation of style, length of flowers, and polarity development of lateral organs in flowering plants. Computational methods were utilized to identify members of the YABBY gene family, with Carrot (Daucus carota) 's genome as a foundational reference. The structure of genes, location of the chromosomes, protein motifs and phylogenetic investigation, syntony and transcriptomic analysis, and miRNA targets were analyzed to unmask the hidden structural and functional characteristics YABBY gene family in Carrots. In the following research, it has been concluded that 11 specific YABBY genes irregularly dispersed on all 9 chromosomes and proteins assembled into five subgroups i.e. AtINO, AtCRC, AtYAB5, AtAFO, and AtYAB2, which were created on the well-known classification of Arabidopsis. The wide ranges of YABBY genes in carrots were dispersed due to segmental duplication, which was detected as prevalent when equated to tandem duplication. Transcriptomic analysis showed that one of the DcYABBY genes was highly expressed during anthocyanin pigmentation in carrot taproots. The cis-regulatory elements (CREs) analysis unveiled elements that particularly respond to light, cell cycle regulation, drought induce ability, ABA hormone, seed, and meristem expression. Furthermore, a relative study among Carrot and Arabidopsis genes of the YABBY family indicated 5 sub-families sharing common characteristics. The comprehensive evaluation of YABBY genes in the genome provides a direction for the cloning and understanding of their functional properties in carrots. Our investigations revealed genome-wide distribution and role of YABBY genes in the carrots with best-fit comparison to Arabidopsis thaliana.</p>","PeriodicalId":72427,"journal":{"name":"BMC genomic data","volume":"25 1","pages":"26"},"PeriodicalIF":1.9,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10916311/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140041068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BMC genomic dataPub Date : 2024-03-04DOI: 10.1186/s12863-024-01212-2
Lin-Fang Wu, Wei-Guang Zhu, En-Ping Yu, Hong-Lin Cao, Zheng-Feng Wang
{"title":"Draft genome of Brasenia schreberi, a worldwide distributed and endangered aquatic plant.","authors":"Lin-Fang Wu, Wei-Guang Zhu, En-Ping Yu, Hong-Lin Cao, Zheng-Feng Wang","doi":"10.1186/s12863-024-01212-2","DOIUrl":"10.1186/s12863-024-01212-2","url":null,"abstract":"<p><strong>Objectives: </strong>Brasenia is a monotypic genus in the family of Cabombaceae. The only species, B. schreberi, is a macrophyte distributed worldwide. Because it requires good water quality, it is endangered in China and other countries due to the deterioration of aquatic habitats. The young leaves and stems of B. schreberi are covered by thick mucilage, which has high medical value. As an allelopathic aquatic plant, it can also be used in the management of aquatic weeds. Here, we present its assembled and annotated genome to help shed light on medial and allelopathic substrates and facilitate their conservation.</p><p><strong>Data description: </strong>Genomic DNA and RNA extracted from B. schreberi leaf tissues were used for whole genome and RNA sequencing using a Nanopore and/or MGI sequencer. The assembly was 1,055,148,839 bp in length, with 92 contigs and an N50 of 22,379,495 bp. The repetitive elements in the assembly were 555,442,205 bp. A completeness assessment of the assembly with BUSCO and compleasm indicated 88.4 and 90.9% completeness in the Eudicots database and 95.4 and 96.6% completeness in the Embryphyta database. Gene annotation revealed 67,747 genes that coded for 73,344 proteins.</p>","PeriodicalId":72427,"journal":{"name":"BMC genomic data","volume":"25 1","pages":"24"},"PeriodicalIF":1.9,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10913576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140029670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BMC genomic dataPub Date : 2024-03-04DOI: 10.1186/s12863-024-01213-1
Zhi Liu, Qing Yang, Bingqiang Liu, Chenhui Li, Xiaolei Shi, Yu Wei, Yuefeng Guan, Chunyan Yang, Mengchen Zhang, Long Yan
{"title":"De novo genome assembly of a high-protein soybean variety HJ117.","authors":"Zhi Liu, Qing Yang, Bingqiang Liu, Chenhui Li, Xiaolei Shi, Yu Wei, Yuefeng Guan, Chunyan Yang, Mengchen Zhang, Long Yan","doi":"10.1186/s12863-024-01213-1","DOIUrl":"10.1186/s12863-024-01213-1","url":null,"abstract":"<p><strong>Objectives: </strong>Soybean is an important feed and oil crop in the world due to its high protein and oil content. China has a collection of more than 43,000 soybean germplasm resources, which provides a rich genetic diversity for soybean breeding. However, the rich genetic diversity poses great challenges to the genetic improvement of soybean. This study reports on the de novo genome assembly of HJ117, a soybean variety with high protein content of 52.99%. These data will prove to be valuable resources for further soybean quality improvement research, and will aid in the elucidation of regulatory mechanisms underlying soybean protein content.</p><p><strong>Data description: </strong>We generated a contiguous reference genome of 1041.94 Mb for HJ117 using a combination of Illumina short reads (23.38 Gb) and PacBio long reads (25.58 Gb), with high-quality sequence coverage of approximately 22.44× and 24.55×, respectively. HJ117 was developed through backcross breeding, using Jidou 12 as the recurrent parent and Chamoshidou as the donor parent. The assembly was further assisted by 114.5 Gb Hi-C data (109.9×), resulting in a contig N50 of 19.32 Mb and scaffold N50 of 51.43 Mb. Notably, Core Eukaryotic Genes Mapping Approach (CEGMA) assessment and Benchmarking Universal Single-Copy Orthologs (BUSCO) assessment results indicated that most core eukaryotic genes (97.18%) and genes in the BUSCO dataset (99.4%) were identified, and 96.44% of the genomic sequences were anchored onto twenty pseudochromosomes.</p>","PeriodicalId":72427,"journal":{"name":"BMC genomic data","volume":"25 1","pages":"25"},"PeriodicalIF":0.0,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10913422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140029669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BMC genomic dataPub Date : 2024-02-26DOI: 10.1186/s12863-024-01208-y
Fei Zhu, Jing Lu, Ke Sun, Cao Deng, Yu Xu
{"title":"Polyploidization of Indotyphlops braminus: evidence from isoform-sequencing.","authors":"Fei Zhu, Jing Lu, Ke Sun, Cao Deng, Yu Xu","doi":"10.1186/s12863-024-01208-y","DOIUrl":"10.1186/s12863-024-01208-y","url":null,"abstract":"<p><strong>Background: </strong>Indotyphlops braminus, the only known triploid parthenogenetic snake, is a compelling species for revealing the mechanism of polyploid emergence in vertebrates.</p><p><strong>Methods: </strong>In this study, we applied PacBio isoform sequencing technology to generate the first full-length transcriptome of I. braminus, aiming to improve the understanding of the molecular characteristics of this species.</p><p><strong>Results: </strong>A total of 51,849 nonredundant full-length transcript assemblies (with an N50 length of 2980 bp) from I. braminus were generated and fully annotated using various gene function databases. Our analysis provides preliminary evidence supporting a recent genome duplication event in I. braminus. Phylogenetic analysis indicated that the divergence of I. braminus subgenomes occurred approximately 11.5 ~ 15 million years ago (Mya). The full-length transcript resource generated as part of this research will facilitate transcriptome analysis and genomic evolution studies in the future.</p>","PeriodicalId":72427,"journal":{"name":"BMC genomic data","volume":"25 1","pages":"23"},"PeriodicalIF":0.0,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10895795/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139974785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BMC genomic dataPub Date : 2024-02-21DOI: 10.1186/s12863-024-01205-1
Jun Dai, Yingyu Zhang, Yunpeng Zhang, Yan Wang, Xiaoyuan Ding, Cheng Song
{"title":"Data notes on the proteomics of Dendrobium huoshanense under pb treatment.","authors":"Jun Dai, Yingyu Zhang, Yunpeng Zhang, Yan Wang, Xiaoyuan Ding, Cheng Song","doi":"10.1186/s12863-024-01205-1","DOIUrl":"10.1186/s12863-024-01205-1","url":null,"abstract":"<p><strong>Objectives: </strong>Pb stress has a negative impact on plant growth by interfering with photosynthesis and releasing reactive oxygen species, causing major risks such as heavy metal ion accumulation in the soil matrix. A proteomics experiment was conducted to determine whether protein levels of Dendrobium huoshanense changed in response to Pb stress seven to fifteen days after being sprayed with a 200 mg/L Pb (NO<sub>3</sub>)<sub>2</sub> solution. The proteomic data we gathered provides a model for investigations into the mechanisms underlying Dendrobium plant resistance to heavy metal stress.</p><p><strong>Data description: </strong>A label-free quantitative proteomics approach was employed to examine the variations in protein expression levels of D. huoshanense at different times of Pb(NO<sub>3</sub>)<sub>2</sub> treatment. We submitted the raw data obtained from these proteomics sequencing experiments to the ProteomeXchange database with the accession number PXD047050. 63,194 mass spectra in total were compared after being imported into the Proteome Discoverer software for database search. A total of 12,402 spectral peptides were identified with a confidence level exceeding 99%, which resulted in the identification of 2,449 significantly differential proteins. These proteins can be utilized for screening, functional annotation, and enrichment analysis of differentially expressed proteins before and after heavy metal treatment experiments.</p>","PeriodicalId":72427,"journal":{"name":"BMC genomic data","volume":"25 1","pages":"22"},"PeriodicalIF":0.0,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10882729/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139934536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BMC genomic dataPub Date : 2024-02-21DOI: 10.1186/s12863-024-01207-z
M Arabfard, N Tajeddin, S Alizadeh, M Salesi, H Bayat, H R Khorram Khorshid, S Khamse, A Delbari, M Ohadi
{"title":"Dyads of GGC and GCC form hotspot colonies that coincide with the evolution of human and other great apes.","authors":"M Arabfard, N Tajeddin, S Alizadeh, M Salesi, H Bayat, H R Khorram Khorshid, S Khamse, A Delbari, M Ohadi","doi":"10.1186/s12863-024-01207-z","DOIUrl":"10.1186/s12863-024-01207-z","url":null,"abstract":"<p><strong>Background: </strong>GGC and GCC short tandem repeats (STRs) are of various evolutionary, biological, and pathological implications. However, the fundamental two-repeats (dyads) of these STRs are widely unexplored.</p><p><strong>Results: </strong>On a genome-wide scale, we mapped (GGC)2 and (GCC)2 dyads in human, and found monumental colonies (distance between each dyad < 500 bp) of extraordinary density, and in some instances periodicity. The largest (GCC)2 and (GGC)2 colonies were intergenic, homogeneous, and human-specific, consisting of 219 (GCC)2 on chromosome 2 (probability < 1.545E-219) and 70 (GGC)2 on chromosome 9 (probability = 1.809E-148). We also found that several colonies were shared in other great apes, and directionally increased in density and complexity in human, such as a colony of 99 (GCC)2 on chromosome 20, that specifically expanded in great apes, and reached maximum complexity in human (probability 1.545E-220). Numerous other colonies of evolutionary relevance in human were detected in other largely overlooked regions of the genome, such as chromosome Y and pseudogenes. Several of the genes containing or nearest to those colonies were divergently expressed in human.</p><p><strong>Conclusion: </strong>In conclusion, (GCC)2 and (GGC)2 form unprecedented genomic colonies that coincide with the evolution of human and other great apes. The extent of the genomic rearrangements leading to those colonies support overlooked recombination hotspots, shared across great apes. The identified colonies deserve to be studied in mechanistic, evolutionary, and functional platforms.</p>","PeriodicalId":72427,"journal":{"name":"BMC genomic data","volume":"25 1","pages":"21"},"PeriodicalIF":0.0,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10880355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139934537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BMC genomic dataPub Date : 2024-02-20DOI: 10.1186/s12863-024-01201-5
Harleen Kaur, Pooja Manchanda, Gurupkar S Sidhu, Parveen Chhuneja
{"title":"Genome-wide identification and characterization of flowering genes in Citrus sinensis (L.) Osbeck: a comparison among C. Medica L., C. Reticulata Blanco, C. Grandis (L.) Osbeck and C. Clementina.","authors":"Harleen Kaur, Pooja Manchanda, Gurupkar S Sidhu, Parveen Chhuneja","doi":"10.1186/s12863-024-01201-5","DOIUrl":"10.1186/s12863-024-01201-5","url":null,"abstract":"<p><strong>Background: </strong>Flowering plays an important role in completing the reproductive cycle of plants and obtaining next generation of plants. In case of citrus, it may take more than a year to achieve progeny. Therefore, in order to fasten the breeding processes, the juvenility period needs to be reduced. The juvenility in plants is regulated by set of various flowering genes. The citrus fruit and leaves possess various medicinal properties and are subjected to intensive breeding programs to produce hybrids with improved quality traits. In order to break juvenility in Citrus, it is important to study the role of flowering genes. The present study involved identification of genes regulating flowering in Citrus sinensis L. Osbeck via homology based approach. The structural and functional characterization of these genes would help in targeting genome editing techniques to induce mutations in these genes for producing desirable results.</p><p><strong>Results: </strong>A total of 43 genes were identified which were located on all the 9 chromosomes of citrus. The in-silico analysis was performed to determine the genetic structure, conserved motifs, cis-regulatory elements (CREs) and phylogenetic relationship of the genes. A total of 10 CREs responsible for flowering were detected in 33 genes and 8 conserved motifs were identified in all the genes. The protein structure, protein-protein interaction network and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was performed to study the functioning of these genes which revealed the involvement of flowering proteins in circadian rhythm pathways. The gene ontology (GO) and gene function analysis was performed to functionally annotate the genes. The structure of the genes and proteins were also compared among other Citrus species to study the evolutionary relationship among them. The expression study revealed the expression of flowering genes in floral buds and ovaries. The qRT-PCR analysis revealed that the flowering genes were highly expressed in bud stage, fully grown flower and early stage of fruit development.</p><p><strong>Conclusions: </strong>The findings suggested that the flowering genes were highly conserved in citrus species. The qRT-PCR analysis revealed the tissue specific expression of flowering genes (CsFT, CsCO, CsSOC, CsAP, CsSEP and CsLFY) which would help in easy detection and targeting of genes through various forward and reverse genetic approaches.</p>","PeriodicalId":72427,"journal":{"name":"BMC genomic data","volume":"25 1","pages":"20"},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10880302/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139914204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}