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De novo transcriptome assembly of the oak processionary moth Thaumetopoea processionea. 橡树游蛾 Thaumetopoea processionea 的全新转录组组装。
BMC genomic data Pub Date : 2024-06-08 DOI: 10.1186/s12863-024-01237-7
Johan Zicola, Prasad Dasari, Katharina Klara Hahn, Katharina Ziese-Kubon, Armin Meurer, Timo Buhl, Stefan Scholten
{"title":"De novo transcriptome assembly of the oak processionary moth Thaumetopoea processionea.","authors":"Johan Zicola, Prasad Dasari, Katharina Klara Hahn, Katharina Ziese-Kubon, Armin Meurer, Timo Buhl, Stefan Scholten","doi":"10.1186/s12863-024-01237-7","DOIUrl":"10.1186/s12863-024-01237-7","url":null,"abstract":"<p><strong>Objectives: </strong>The oak processionary moth (OPM) (Thaumetopoea processionea) is a species of moth (order: Lepidoptera) native to parts of central Europe. However, in recent years, it has become an invasive species in various countries, particularly in the United Kingdom and the Netherlands. The larvae of the OPM are covered with urticating barbed hairs (setae) causing irritating and allergic reactions at the three last larval stages (L3-L5). The aim of our study was to generate a de novo transcriptomic assembly for OPM larvae by including one non-allergenic stage (L2) and two allergenic stages (L4 and L5). A transcriptomic assembly will help identify potential allergenic peptides produced by OPM larvae, providing valuable information for developing novel therapeutic strategies and allergic immunodiagnostic assays.</p><p><strong>Data: </strong>Transcriptomes of three larval stages of the OPM were de novo assembled and annotated using Trinity and Trinotate, respectively. A total of 145,251 transcripts from 99,868 genes were identified. Bench-marking universal single-copy orthologues analysis indicated high completeness of the assembly. About 19,600 genes are differentially expressed between the non-allergenic and allergenic larval stages. The data provided here contribute to the characterization of OPM, which is both an invasive species and a health hazard.</p>","PeriodicalId":72427,"journal":{"name":"BMC genomic data","volume":"25 1","pages":"55"},"PeriodicalIF":0.0,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11161914/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141294002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transcriptome analysis revealed differences in gene expression in sheep muscle tissue at different developmental stages. 转录组分析显示,绵羊肌肉组织在不同发育阶段的基因表达存在差异。
BMC genomic data Pub Date : 2024-06-07 DOI: 10.1186/s12863-024-01235-9
Sailuo Wan, Mengyu Lou, Sihuan Zhang, Shuang Li, Yinghui Ling
{"title":"Transcriptome analysis revealed differences in gene expression in sheep muscle tissue at different developmental stages.","authors":"Sailuo Wan, Mengyu Lou, Sihuan Zhang, Shuang Li, Yinghui Ling","doi":"10.1186/s12863-024-01235-9","DOIUrl":"10.1186/s12863-024-01235-9","url":null,"abstract":"<p><strong>Background: </strong>The analysis of differentially expressed genes in muscle tissues of sheep at different ages is helpful to analyze the gene expression trends during muscle development. In this study, the longissimus dorsi muscle of pure breeding Hu sheep (H), Suffolk sheep and Hu sheep hybrid F1 generation (SH) and East Friesian and Hu sheep hybrid sheep (EHH) three strains of sheep born 2 days (B2) and 8 months (M8) was used as the research object, and transcriptome sequencing technology was used to identify the differentially expressed genes of sheep longissimus dorsi muscle in these two stages. Subsequently, GO and KEGG enrichment analysis were performed on the differential genes. Nine differentially expressed genes were randomly selected and their expression levels were verified by qRT-PCR.</p><p><strong>Results: </strong>The results showed that 842, 1301 and 1137 differentially expressed genes were identified in H group, SH group and EHH group, respectively. Among them, 191 differential genes were enriched in these three strains, including pre-folding protein subunit 6 (PFDN6), DnaJ heat shock protein family member A4 (DNAJA4), myosin heavy chain 8 (MYH8) and so on. GO and KEGG enrichment analysis was performed on 191 differentially expressed genes shared by the three strains to determine common biological pathways. The results showed that the differentially expressed genes were significantly enriched in ribosomes, unfolded protein binding, FoxO signaling pathway, glycolysis / glycogen generation and glutathione signaling pathway that regulate muscle protein synthesis and energy metabolism. The results of qRT-PCR were consistent with transcriptome sequencing, which proved that the sequencing results were reliable.</p><p><strong>Conclusions: </strong>Overall, this study revealed the important genes and signaling pathways related to sheep skeletal muscle development, and the result laid a foundation for further understanding the mechanism of sheep skeletal muscle development.</p>","PeriodicalId":72427,"journal":{"name":"BMC genomic data","volume":"25 1","pages":"54"},"PeriodicalIF":0.0,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11162047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141289012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome sequencing of captive white tigers from Bangladesh. 孟加拉国圈养白虎的基因组测序。
BMC genomic data Pub Date : 2024-06-06 DOI: 10.1186/s12863-024-01239-5
Ashutosh Das, Md Shahadat Hossain Suvo, Mishuk Shaha, Mukta Das Gupta
{"title":"Genome sequencing of captive white tigers from Bangladesh.","authors":"Ashutosh Das, Md Shahadat Hossain Suvo, Mishuk Shaha, Mukta Das Gupta","doi":"10.1186/s12863-024-01239-5","DOIUrl":"10.1186/s12863-024-01239-5","url":null,"abstract":"<p><strong>Objectives: </strong>The Bengal tiger Panthera tigris tigris, is an emblematic animal for Bangladesh. Despite being the apex predator in the wild, their number is decreasing due to anthropogenic activities such as hunting, urbanization, expansion of agriculture and deforestation. By contrast, captive tigers are flourishing due to practical conservation efforts. Breeding within the small captive population can produce inbreeding depression and genetic bottlenecks, which may limit the success of conservation efforts. Despite past decades of research, a comprehensive database on genetic variation in the captive and wild Bengal tigers in Bangladesh still needs to be included. Therefore, this research aimed to investigate the White Bengal tiger genome to create a resource for future studies to understand variation underlying important functional traits.</p><p><strong>Data description: </strong>Blood samples from Chattogram Zoo were collected for three white Bengal tigers. Genomic DNA for all collected samples were extracted using a commercial DNA extraction kit. Whole genome sequencing was performed using a DNBseq platform. We generated 77 Gb of whole-genome sequencing (WGS) data for three white Bengal tigers (Average 11X coverage/sample). The data we generated will establish a paradigm for tiger research in Bangladesh by providing a genomic resource for future functional studies on the Bengal white tiger.</p>","PeriodicalId":72427,"journal":{"name":"BMC genomic data","volume":"25 1","pages":"52"},"PeriodicalIF":0.0,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11155014/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141285540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Draft assembly and annotation of the Cuban crocodile (Crocodylus rhombifer) genome. 古巴鳄(Crocodylus rhombifer)基因组的组装和注释草案。
BMC genomic data Pub Date : 2024-06-06 DOI: 10.1186/s12863-024-01240-y
Robert W Meredith, Yoamel Milián-García, John Gatesy, Michael A Russello, George Amato
{"title":"Draft assembly and annotation of the Cuban crocodile (Crocodylus rhombifer) genome.","authors":"Robert W Meredith, Yoamel Milián-García, John Gatesy, Michael A Russello, George Amato","doi":"10.1186/s12863-024-01240-y","DOIUrl":"10.1186/s12863-024-01240-y","url":null,"abstract":"<p><strong>Objectives: </strong>The new data provide an important genomic resource for the Critically Endangered Cuban crocodile (Crocodylus rhombifer). Cuban crocodiles are restricted to the Zapata Swamp in southern Matanzas Province, Cuba, and readily hybridize with the widespread American crocodile (Crocodylus acutus) in areas of sympatry. The reported de novo assembly will contribute to studies of crocodylian evolutionary history and provide a resource for informing Cuban crocodile conservation.</p><p><strong>Data description: </strong>The final 2.2 Gb draft genome for C. rhombifer consists of 41,387 scaffolds (contigs: N50 = 104.67 Kb; scaffold: N50-518.55 Kb). Benchmarking Universal Single-Copy Orthologs (BUSCO) identified 92.3% of the 3,354 genes in the vertebrata_odb10 database. Approximately 42% of the genome (960Mbp) comprises repeat elements. We predicted 30,138 unique protein-coding sequences (17,737 unique genes) in the genome assembly. Functional annotation found the top Gene Ontology annotations for Biological Processes, Molecular Function, and Cellular Component were regulation, protein, and intracellular, respectively. This assembly will support future macroevolutionary, conservation, and molecular studies of the Cuban crocodile.</p>","PeriodicalId":72427,"journal":{"name":"BMC genomic data","volume":"25 1","pages":"53"},"PeriodicalIF":0.0,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11157745/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141285539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome-wide association study of BNT162b2 vaccine-related myocarditis identifies potential predisposing functional areas in Hong Kong adolescents. BNT162b2 疫苗相关心肌炎的全基因组关联研究确定了香港青少年的潜在易感功能区。
BMC genomic data Pub Date : 2024-06-06 DOI: 10.1186/s12863-024-01238-6
Chun Hing She, Hing Wai Tsang, Xingtian Yang, Sabrina Sl Tsao, Clara Sm Tang, Sophelia Hs Chan, Mike Yw Kwan, Gilbert T Chua, Wanling Yang, Patrick Ip
{"title":"Genome-wide association study of BNT162b2 vaccine-related myocarditis identifies potential predisposing functional areas in Hong Kong adolescents.","authors":"Chun Hing She, Hing Wai Tsang, Xingtian Yang, Sabrina Sl Tsao, Clara Sm Tang, Sophelia Hs Chan, Mike Yw Kwan, Gilbert T Chua, Wanling Yang, Patrick Ip","doi":"10.1186/s12863-024-01238-6","DOIUrl":"10.1186/s12863-024-01238-6","url":null,"abstract":"<p><p>Vaccine-related myocarditis associated with the BNT162b2 vaccine is a rare complication, with a higher risk observed in male adolescents. However, the contribution of genetic factors to this condition remains uncertain. In this study, we conducted a comprehensive genetic association analysis in a cohort of 43 Hong Kong Chinese adolescents who were diagnosed with myocarditis shortly after receiving the BNT162b2 mRNA COVID-19 vaccine. A comparison of whole-genome sequencing data was performed between the confirmed myocarditis cases and a control group of 481 healthy individuals. To narrow down potential genomic regions of interest, we employed a novel clustering approach called ClusterAnalyzer, which prioritised 2,182 genomic regions overlapping with 1,499 genes for further investigation. Our pathway analysis revealed significant enrichment of these genes in functions related to cardiac conduction, ion channel activity, plasma membrane adhesion, and axonogenesis. These findings suggest a potential genetic predisposition in these specific functional areas that may contribute to the observed side effect of the vaccine. Nevertheless, further validation through larger-scale studies is imperative to confirm these findings. Given the increasing prominence of mRNA vaccines as a promising strategy for disease prevention and treatment, understanding the genetic factors associated with vaccine-related myocarditis assumes paramount importance. Our study provides valuable insights that significantly advance our understanding in this regard and serve as a valuable foundation for future research endeavours in this field.</p>","PeriodicalId":72427,"journal":{"name":"BMC genomic data","volume":"25 1","pages":"51"},"PeriodicalIF":0.0,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11155081/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141285541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quantifying variations associated with dental caries reveals disparity in effect allele frequencies across diverse populations. 通过量化与龋齿相关的变异,可以发现不同人群中影响等位基因频率的差异。
IF 1.9
BMC genomic data Pub Date : 2024-06-03 DOI: 10.1186/s12863-024-01215-z
Sangram Sandhu, Varun Sharma, Sachin Kumar, Niraj Rai, Pooran Chand
{"title":"Quantifying variations associated with dental caries reveals disparity in effect allele frequencies across diverse populations.","authors":"Sangram Sandhu, Varun Sharma, Sachin Kumar, Niraj Rai, Pooran Chand","doi":"10.1186/s12863-024-01215-z","DOIUrl":"10.1186/s12863-024-01215-z","url":null,"abstract":"<p><strong>Background: </strong>Dental caries (DC) is a multifaceted oral condition influenced by genetic and environmental factors. Recent advancements in genotyping and sequencing technologies, such as Genome-Wide Association Studies (GWAS) have helped researchers to identify numerous genetic variants associated with DC, but their prevalence and significance across diverse global populations remain poorly understood as most of the studies were conducted in European populations, and very few were conducted in Asians specifically in Indians.</p><p><strong>Aim: </strong>This study aimed to evaluate the genetic affinity of effect alleles associated with DC to understand the genetic relationship between global populations with respect to the Indian context.</p><p><strong>Methodology: </strong>This present study used an empirical approach in which variants associated with DC susceptibility were selected. These variants were identified and annotated using the GWAS summary. The genetic affinity was evaluated using Fst.</p><p><strong>Results: </strong>The effect of allele frequencies among different populations was examined, revealing variations in allele distribution. African populations exhibited higher frequencies of specific risk alleles, whereas East Asian and European populations displayed distinct profiles. South Asian populations showed a unique genetic cluster.</p><p><strong>Conclusion: </strong>Our study emphasises the complex genetic landscape of DC and highlights the need for population-specific research as well as validation of GWAS-identified markers in Indians before defining them as established candidate genes.</p>","PeriodicalId":72427,"journal":{"name":"BMC genomic data","volume":"25 1","pages":"50"},"PeriodicalIF":1.9,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11149341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141238857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative chloroplast genomics and phylogenetic analysis of Oreomecon nudicaulis (Papaveraceae). Oreomecon nudicaulis(木瓜科)叶绿体比较基因组学和系统发育分析。
BMC genomic data Pub Date : 2024-05-30 DOI: 10.1186/s12863-024-01236-8
Qingbin Zhan, Yalin Huang, Xiaoming Xue, Yunxia Chen
{"title":"Comparative chloroplast genomics and phylogenetic analysis of Oreomecon nudicaulis (Papaveraceae).","authors":"Qingbin Zhan, Yalin Huang, Xiaoming Xue, Yunxia Chen","doi":"10.1186/s12863-024-01236-8","DOIUrl":"10.1186/s12863-024-01236-8","url":null,"abstract":"<p><p>Oreomecon nudicaulis, commonly known as mountain poppy, is a significant perennial herb. In 2022, the species O. nudicaulis, which was previously classified under the genus Papaver, was reclassified within the genus Oreomecon. Nevertheless, the phylogenetic status and chloroplast genome within the genus Oreomecon have not yet been reported. This study elucidates the chloroplast genome sequence and structural features of O. nudicaulis and explores its evolutionary relationships within Papaveraceae. Using Illumina sequencing technology, the chloroplast genome of O. nudicaulis was sequenced, assembled, and annotated. The results indicate that the chloroplast genome of O. nudicaulis exhibits a typical circular quadripartite structure. The chloroplast genome is 153,903 bp in length, with a GC content of 38.87%, containing 84 protein-coding genes, 8 rRNA genes, 38 tRNA genes, and 2 pseudogenes. The genome encodes 25,815 codons, with leucine (Leu) being the most abundant codon, and the most frequently used codon is AUU. Additionally, 129 microsatellite markers were identified, with mononucleotide repeats being the most abundant (53.49%). Our phylogenetic analysis revealed that O. nudicaulis has a relatively close relationship with the genus Meconopsis within the Papaveraceae family. The phylogenetic analysis supported the taxonomic status of O. nudicaulis, as it did not form a clade with other Papaver species, consistent with the revised taxonomy of Papaveraceae. This is the first report of a phylogenomic study of the complete chloroplast genome in the genus Oreomecon, which is a significant genus worldwide. This analysis of the O. nudicaulis chloroplast genome provides a theoretical basis for research on genetic diversity, molecular marker development, and species identification, enriching genetic information and supporting the evolutionary relationships among Papaveraceae.</p>","PeriodicalId":72427,"journal":{"name":"BMC genomic data","volume":"25 1","pages":"49"},"PeriodicalIF":0.0,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11141030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141181657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome sequence of two novel virulent clinical strains of Burkholderia pseudomallei isolated from acute melioidosis cases imported to Israel from India and Thailand. 从印度和泰国输入以色列的急性瓜虫病病例中分离出的两株新型假马利伯克霍尔德氏菌临床毒株的基因组序列。
BMC genomic data Pub Date : 2024-05-23 DOI: 10.1186/s12863-024-01225-x
Inbar Cohen-Gihon, Galia Zaide, Sharon Amit, Iris Zohar, Orna Schwartz, Yasmin Maor, Ofir Israeli, Gal Bilinsky, Ma'ayan Israeli, Shirley Lazar, David Gur, Moshe Aftalion, Anat Zvi, Adi Beth-Din, Erez Bar-Haim, Uri Elia, Ofer Cohen, Emanuelle Mamroud, Theodor Chitlaru
{"title":"Genome sequence of two novel virulent clinical strains of Burkholderia pseudomallei isolated from acute melioidosis cases imported to Israel from India and Thailand.","authors":"Inbar Cohen-Gihon, Galia Zaide, Sharon Amit, Iris Zohar, Orna Schwartz, Yasmin Maor, Ofir Israeli, Gal Bilinsky, Ma'ayan Israeli, Shirley Lazar, David Gur, Moshe Aftalion, Anat Zvi, Adi Beth-Din, Erez Bar-Haim, Uri Elia, Ofer Cohen, Emanuelle Mamroud, Theodor Chitlaru","doi":"10.1186/s12863-024-01225-x","DOIUrl":"10.1186/s12863-024-01225-x","url":null,"abstract":"<p><strong>Objective: </strong>Burkholderia pseudomallei, the etiological cause of melioidosis, is a soil saprophyte endemic in South-East Asia, where it constitutes a public health concern of high-priority. Melioidosis cases are sporadically identified in nonendemic areas, usually associated with travelers or import of goods from endemic regions. Due to extensive intercontinental traveling and the anticipated climate change-associated alterations of the soil bacterial flora, there is an increasing concern for inadvertent establishment of novel endemic areas, which may expand the global burden of melioidosis. Rapid diagnosis, isolation and characterization of B. pseudomallei isolates is therefore of utmost importance particularly in non-endemic locations.</p><p><strong>Data description: </strong>We report the genome sequences of two novel clinical isolates (MWH2021 and MST2022) of B. pseudomallei identified in distinct acute cases of melioidosis diagnosed in two individuals arriving to Israel from India and Thailand, respectively. The data includes preliminary genetic analysis of the genomes determining their phylogenetic classification in rapport to the genomes of 131 B. pseudomallei strains documented in the NCBI database. Inspection of the genomic data revealed the presence or absence of loci encoding for several documented virulence determinants involved in the molecular pathogenesis of melioidosis. Virulence analysis in murine models of acute or chronic melioidosis established that both strains belong to the highly virulent class of B. pseudomalleii.</p>","PeriodicalId":72427,"journal":{"name":"BMC genomic data","volume":"25 1","pages":"47"},"PeriodicalIF":0.0,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11118722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141089366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Combined full-length transcriptomic and metabolomic analysis reveals the molecular mechanisms underlying nutrients and taste components development in Primulina juliae. 结合全长转录组学和代谢组学分析,揭示茱莉叶报春花营养成分和味觉成分发育的分子机制。
BMC genomic data Pub Date : 2024-05-23 DOI: 10.1186/s12863-024-01231-z
Yi Zhang, Endian Yang, Qin Liu, Jie Zhang, Chen Feng
{"title":"Combined full-length transcriptomic and metabolomic analysis reveals the molecular mechanisms underlying nutrients and taste components development in Primulina juliae.","authors":"Yi Zhang, Endian Yang, Qin Liu, Jie Zhang, Chen Feng","doi":"10.1186/s12863-024-01231-z","DOIUrl":"10.1186/s12863-024-01231-z","url":null,"abstract":"<p><strong>Background: </strong>Primulina juliae has recently emerged as a novel functional vegetable, boasting a significant biomass and high calcium content. Various breeding strategies have been employed to the domestication of P. juliae. However, the absence of genome and transcriptome information has hindered the research of mechanisms governing the taste and nutrients in this plant. In this study, we conducted a comprehensive analysis, combining the full-length transcriptomics and metabolomics, to unveil the molecular mechanisms responsible for the development of nutrients and taste components in P. juliae.</p><p><strong>Results: </strong>We obtain a high-quality reference transcriptome of P. juliae by combing the PacBio Iso-seq and Illumina sequencing technologies. A total of 58,536 cluster consensus sequences were obtained, including 28,168 complete protein coding transcripts and 8,021 Long Non-coding RNAs. Significant differences were observed in the composition and content of compounds related to nutrients and taste, particularly flavonoids, during the leaf development. Our results showed a decrease in the content of most flavonoids as leaves develop. Malate and succinate accumulated with leaf development, while some sugar metabolites were decreased. Furthermore, we identified the different accumulation of amino acids and fatty acids, which are associated with taste traits. Moreover, our transcriptomic analysis provided a molecular basis for understanding the metabolic variations during leaf development. We identified 4,689 differentially expressed genes in the two developmental stages, and through a comprehensive transcriptome and metabolome analysis, we discovered the key structure genes and transcription factors involved in the pathways.</p><p><strong>Conclusions: </strong>This study provides a high-quality reference transcriptome and reveals molecular mechanisms associated with the development of nutrients and taste components in P. juliae. These findings will enhance our understanding of the breeding and utilization of P. juliae as a vegetable.</p>","PeriodicalId":72427,"journal":{"name":"BMC genomic data","volume":"25 1","pages":"46"},"PeriodicalIF":0.0,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11112898/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141089363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome assembly of Ottelia alismoides, a multiple-carbon utilisation aquatic plant. 多种碳利用水生植物 Ottelia alismoides 的基因组组装。
IF 1.9
BMC genomic data Pub Date : 2024-05-23 DOI: 10.1186/s12863-024-01230-0
Zheng-Feng Wang, Lin-Fang Wu, Lei Chen, Wei-Guang Zhu, En-Ping Yu, Feng-Xia Xu, Hong-Lin Cao
{"title":"Genome assembly of Ottelia alismoides, a multiple-carbon utilisation aquatic plant.","authors":"Zheng-Feng Wang, Lin-Fang Wu, Lei Chen, Wei-Guang Zhu, En-Ping Yu, Feng-Xia Xu, Hong-Lin Cao","doi":"10.1186/s12863-024-01230-0","DOIUrl":"10.1186/s12863-024-01230-0","url":null,"abstract":"<p><strong>Objectives: </strong>Ottelia Pers. is in the Hydrocharitaceae family. Species in the genus are aquatic, and China is their centre of origin in Asia. Ottelia alismoides (L.) Pers., which is distributed worldwide, is a distinguishing element in China, while other species of this genus are endemic to China. However, O. alismoides is also considered endangered due to habitat loss and pollution in some Asian countries. Ottelia alismoides is the only submerged macrophyte that contains three carbon dioxide-concentrating mechanisms, i.e. bicarbonate (HCO<sub>3</sub><sup>-</sup>) use, crassulacean acid metabolism and the C4 pathway. In this study, we present its first genome assembly to help illustrate the various carbon metabolism mechanisms and to enable genetic conservation in the future.</p><p><strong>Data description: </strong>Using DNA and RNA extracted from one O. alismoides leaf, this work produced ∼ 73.4 Gb HiFi reads, ∼ 126.4 Gb whole genome sequencing short reads and ∼ 21.9 Gb RNA-seq reads. The de novo genome assembly was 6,455,939,835 bp in length, with 11,923 scaffolds/contigs and an N50 of 790,733 bp. Genome assembly completeness assessment with Benchmarking Universal Single-Copy Orthologs revealed a score of 94.4%. The repetitive sequence in the assembly was 4,875,817,144 bp (75.5%). A total of 116,176 genes were predicted. The protein sequences were functionally annotated against multiple databases, facilitating comparative genomic analysis.</p>","PeriodicalId":72427,"journal":{"name":"BMC genomic data","volume":"25 1","pages":"48"},"PeriodicalIF":1.9,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11118731/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141089365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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